Abstract：
:A freeze-etch replica method combined with biochemical analyses was used to investigate the ultrastructural organization of the bovine Descemet's membrane. The freeze-etch replica observations revealed that the intact Descemet's membranes were composed of stacks of two-dimensionally arranged hexagonal lattices, in which four components were resolved; (1) round densities as nodes, (2) rod-like structures connecting the densities, (3) randomly oriented fine filaments within the lattices, and (4) amorphous materials covering the lattices. When the membranes were treated with sodium dodecyl sulfate (SDS) and mercaptoethanol, only the amorphous materials were solubilized. However, both the amorphous materials and rod-like structures disappeared in SDS-mercaptoethanol-urea-treated membranes. When the membranes were treated with a very low concentration (0.0005%) of collagenase, rod-like structures and round densities remained insoluble. If the concentration was raised to 0.01%, only the round densities persisted. Comparing these data with the amino acid analysis of each fraction, the following conclusions may be drawn: rod-like structures and fine filaments contain collagenous proteins of different solubility, while round densities and amorphous materials are non-collagenous in nature.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Sawada Hdoi
10.1007/BF00218356subject
Has Abstractpub_date
1982-01-01 00:00:00pages
241-55issue
2eissn
0302-766Xissn
1432-0878journal_volume
226pub_type
杂志文章abstract：:The fine structure of luminal surface of clearly identified portions of uriniferous tubules has been studied by scanning electron microscopy to elucidate some controversies concerning the topography of certain surface formations. The results show a characteristic pattern of the luminal surface in the region of Henle's...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215128
更新日期：1976-02-06 00:00:00
abstract：:Scanning electron microscopy was used to investigate the morphological changes of the tail musculature of the metamorphosing anuran tadpole, attention being focused on phagocytosis by macrophages. Muscle fibers were stained en bloc with silver and freeze-fractured during dehydration, or torn after drying. Samples were...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214574
更新日期：1985-01-01 00:00:00
abstract：:The purpose of this study was to determine the cellular distribution and degradation in rat liver following intravenous injection of superparamagnetic iron oxide nanoparticles used for magnetic resonance imaging (NC100150 Injection). Relaxometric and spectrophotometric methods were used to determine the concentration ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0884-8
更新日期：2004-06-01 00:00:00
abstract：:A quantitative evaluation of putative amino acid neurotransmitters in sea pansy polyps by high-performance liquid chromatography indicates that the taurine content exceeds that of other amino acids by a 100-fold. The cellular source of this taurine was investigated by immunohistochemistry with two polyclonal antisera ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050800
更新日期：1997-04-01 00:00:00
abstract：:Expression of the non-muscle myosin heavy chain-B (NM-MHC-B, also denoted as the embryonic smooth muscle myosin heavy chain, SMemb) was examined in rat urinary bladder during growth in response to a partial urinary outflow obstruction. Following obstruction, the weight of the urinary bladder increased more than five-f...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000262
更新日期：2001-05-01 00:00:00
abstract：:Dysfunction of the retinal pigmented epithelium (RPE) is one of the first effects of dry age-related macular degeneration (AMD) with consequent blindness. Hence, patients affected by this retinal disorder could benefit from a cell-based transplantation strategy for RPE. Actually, an effective protocol to approach this...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-015-2185-9
更新日期：2015-10-01 00:00:00
abstract：:Their strategic location between blood and tissue and their constitutive properties allow endothelial cells (EC) to monitor the transport of plasma molecules, by employing bidirectional receptor-mediated and receptor-independent transcytosis and endocytosis, and to regulate vascular tone, cellular cholesterol and lipi...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-008-0678-5
更新日期：2009-01-01 00:00:00
abstract：:Gap junctions in the phylum Brachiopoda are shown to be of the P type as has been previously reported in invertebrates only in molluscs and possibly bryozoans. However, the mollusc gap junctions appear identical to vertebrae gap junctions in the fracture face on which particles remain, in particle spacing and in parti...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00206345
更新日期：1982-01-01 00:00:00
abstract：:S-antigen-immunoreactive pinealocytes located in the deep portion of the pineal organ of inbred and wild pigmented mice give rise to long, beaded processes penetrating into the habenular and pretectal regions. In addition, the medial habenular nuclei and the pretectal area contain S-antigen-immunoreactive perikarya, w...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00313528
更新日期：1990-09-01 00:00:00
abstract：:Kidneys of 2 to 10 day-old rats of Wistar and Sprague-Dawley strains were fixed with glutaraldehyde by retrograde vascular perfusion and then prepared for observation in TEM and SEM. In addition methacrylate casts of differentiating glomerular capillaries were examined by SEM. Although the glomerular vascular pattern ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233959
更新日期：1980-01-01 00:00:00
abstract：:The prolactin-producing cells are the first hormone-producing cells of the pars distalis to be differentiated within Ambystoma gracile. They first appear when the larvae attain a length of approximately 3.0 cm snout to vent length (SVL). Thyrotropes are observed as the next chromophilic cells to appear, and they occur...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00223121
更新日期：1978-05-18 00:00:00
abstract：:Previous studies have indicated that 19-day-old fetal long bones of the rat contain an adenylyl cyclase-stimulating activity antigenically related to parathyroid hormone-related peptide. To ascertain its origin, Northern blotting and in situ hybridization histochemistry were performed. Results demonstrate that mRNA of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00645063
更新日期：1992-12-01 00:00:00
abstract：:The otoconia of Amphibia, especially of the frog Rana esculenta, were studied by X-ray diffraction and scanning electron microscopy (SEM). The SEM studies showed that the membranous labyrinth of Amphibia contains two populations of crystals, which can easily be distinguished by their forms. The X-ray diffraction data ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222230
更新日期：1983-01-01 00:00:00
abstract：:While brain-derived neurotrophic factor (BDNF) messenger RNA (mRNA) has been localized in the developing gustatory epithelium, little information is available about neurotrophin-3 (NT-3) mRNA expression pattern in the prenatal developing gustatory and lingual epithelium. In the present study, using in situ hybridizati...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051092
更新日期：1998-06-01 00:00:00
abstract：:Previous studies have revealed a remarkable capacity of intracerebral grafts of embryonic brain tissue to establish extensive axonal connections with denervated areas in the brains of adult rats. In the present study we have explored the possibilities of using grafts in the spinal cord to substitute for the loss of no...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216024
更新日期：1983-01-01 00:00:00
abstract：:Subsurface cisterns (SSC's) and less frequent lamellar bodies (LB's) were identified in the granule cells of the guinea-pig fascia dentata. Both structures, composed of flattened or collapsed agranular cisterns, are continuous with the regular rough endoplasmic reticulum and occasionally connected with each other form...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF01258502
更新日期：1982-01-01 00:00:00
abstract：:The innervation of the rat anococcygeus muscle has been investigated ultrastructurally following fixation with a modified chromaffin reaction for the demonstration of biogenic amines (Tranzer and Richards, 1976). Three types of nerve profiles were revealed: (1) 60-70% of the profiles are adrenergic; (2) less than 5% o...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236418
更新日期：1979-08-01 00:00:00
abstract：:The structure of the bulbus arteriosus of a wide range of teleost fish is described with particular reference to the vascularization and innervation. The adventitia of the organ consists of blood vessels and large nerve bundles in a collagen matrix. The nerve bundles contain monoamines, and fluorescence studies show s...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00240106
更新日期：1979-02-15 00:00:00
abstract：:The attachment of the body of the snail Lymnaea stagnalis to the shell was studied by histochemistry and light and electron microscopy. Muscles of the body wall insert into the connective tissue by way of long thin projections of sarcolemma. The muscle cells end under the basement membrane of a specialised area of the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00224663
更新日期：1976-08-26 00:00:00
abstract：:Colorectal adenocarcinomas were induced in male Wistar rats, by weekly subcutaneous administration of 1,2-dimethylhydrazine, classified according to the degree of differentiation and submitted to immunocytochemistry for the peptides cholecystokinin (CCK), gastrin, gastric inhibitory polypeptide (GIP), glucagon, neurot...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219019
更新日期：1986-01-01 00:00:00
abstract：:The desert locust Schistocerca gregaria is a major agricultural pest in North Africa and the Middle East. As such, it has been intensely studied, in particular with respect to population dynamics, sensory processing, feeding behavior flight and locomotor control, migratory behavior, and its neuroendocrine system. Bein...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2844-8
更新日期：2018-10-01 00:00:00
abstract：:Double-labeling immunofluorescent histochemistry demonstrates that calretinin, a calcium-binding protein, coexists with calcitonin gene-related peptide, vasoactive intestinal peptide, and substance P in the fibers innervating the lamina propria of the rat intestinal villi. An acetylcholinesterase histochemical stain r...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318366
更新日期：1995-06-01 00:00:00
abstract：:This work describes the origin and development of about 200 tension receptor cells located around the anterior attachment site of the locust ovipositor muscle and their migration to their final position on the muscle fibres. The locust ovipositor muscle is the only insect system in which more than 100 tension receptor...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0498-z
更新日期：2007-12-01 00:00:00
abstract：:In developing bioassays for estrogenic effects, vitellogenin (Vtg) induction and zona radiata protein (Zr-protein) induction in males and juveniles of oviparous vertebrates have been used as sensitive biomarkers for estrogenicity. Nonylphenol (NP) produces similar and parallel expression patterns of Vtg and Zr-protein...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0543-y
更新日期：2008-03-01 00:00:00
abstract：:Peeling skin syndrome is a heterogeneous group of rare disorders. Peeling skin, leukonychia, acral punctate keratoses, cheilitis and knuckle pads (PLACK syndrome, OMIM616295) is a newly described form of PSS with an autosomal recessive mode of inheritance. We report a 5.5-year-old boy with features of PLACK syndrome. ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-03077-9
更新日期：2019-11-01 00:00:00
abstract：:Fibres growing from neurons of explanted dorsal root ganglia from 10 day chick embryos were transected and subsequently observed by light and electron microscopy after periods of a few to fifty minutes. Changes immediately proximal and distal to the cut together with alterations further away from the site of injury on...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219562
更新日期：1978-07-10 00:00:00
abstract：:Besides its function at cholinergic synapses, acetylcholinesterase (AChE) exerts structural functions on neural differentiation, independent of its enzymatic activity. To elucidate such functions, we have previously heterologously expressed AChE in histotypic retinal reaggregates, revealing strong effects on their his...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100444
更新日期：2001-11-01 00:00:00
abstract：:Periodontal regeneration requires the coordinated movement and differentiation of several cell types in order to re-establish the cementum, periodontal ligament (PDL), and alveolar bone. Cells in culture are often used as model systems for mature tissues, although they may represent expanded progenitor cell population...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0282-5
更新日期：2007-01-01 00:00:00
abstract：:The axon hillock (AH) and initial segment (IS) of 10 Golgi neurons and 6 basket cells in the cerebellar cortex of the rat were investigated by electron microscopy using serial sections. An average of 10.4 and 11.3 synaptic terminals were observed to establish synaptic contact with the axon hillock region of Golgi and ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219363
更新日期：1981-01-01 00:00:00
abstract：:Immunocytochemistry using antibodies against Met-enkephalin and Leu-enkephalin has demonstrated a group of large enkephalin-immunoreactive neurons in the nucleus of the rostral mesencephalic tegmentum (mRMT) of two teleost fish. Salmo gairdneri and Salmo salar. Injections of cobalt-lysine in the medial optic tectum re...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00305731
更新日期：1991-04-01 00:00:00
