Abstract：
:Besides its function at cholinergic synapses, acetylcholinesterase (AChE) exerts structural functions on neural differentiation, independent of its enzymatic activity. To elucidate such functions, we have previously heterologously expressed AChE in histotypic retinal reaggregates, revealing strong effects on their histogenesis, particularly on Müller glia processes. To further resolve these findings at a less complex cellular level, in this study we transfected adherent retinal cells of the chick embryo after 2 days i.c. with a sense pSVK3-AChErab-cDNA expression vector encoding for the entire rabbit AChE gene by calcium phosphate precipitation. Northern blots using digoxigenin (DIG)-labeled rabbit cDNA revealed a pronounced level of rabbit AChE mRNA in AChE-transfected cells. Western blot analysis established an increase in the endogenous AChE protein in transfected cells. Noticeably, AChE activity was not much affected, indicating a post-translational regulation of overall AChE activity. As a corollary, 5'-bromo-2'-deoxyuridine (BrdU) studies showed a decrease in cell proliferation. Exploring changes of the Müller glia, the cytoskeletal protein vimentin was found to be increased in transfected cells. Vimentin-stained processes are longer, thicker and more orderly arranged. In conclusion, exogenous expression of rabbit AChE in chicken retinal monolayers exerts a structural function on glial cytoskeletal organization, independent of AChE activity.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Keller M,Robitzki A,Layer PGdoi
10.1007/s004410100444keywords:
subject
Has Abstractpub_date
2001-11-01 00:00:00pages
187-98issue
2eissn
0302-766Xissn
1432-0878journal_volume
306pub_type
杂志文章abstract：:Modulation of the actin cytoskeleton in chondrocytes has been used to prevent or reverse dedifferentiation and to enhance protein synthesis. We have hypothesized that an actin-modifying agent, staurosporine, could be used with fibrochondrocytes to increase the gene expression and synthesis of critical fibrocartilage p...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0705-6
更新日期：2008-12-01 00:00:00
abstract：:The localization of neuropeptide Y (NPY) and atrial natriuretic peptide (ANP) in the endothelial cells of human umbilical blood vessels was studied using the pre-embedding peroxidase-antiperoxidase (PAP) technique for electron microscopy and avidin-biotin-complex (ABC) immunostaining for endothelial cells cultured fro...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00323584
更新日期：1993-04-01 00:00:00
abstract：:We demonstrate the existence of nerve fibers possessing substance P (SP) and calcitonin gene-related peptide (CGRP) immunoreactivity in the mouse cervical ventral roots. The distribution of the SP and CGRP fibers was similar, but CGRP fibers were generally more numerous. Both types entered the ventral pia mater or for...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327774
更新日期：1994-08-01 00:00:00
abstract：:The published online version contains mistake in Title and in Acknowledgement section. Please find below for the needed corrections. ...
journal_title：Cell and tissue research
pub_type： 杂志文章,已发布勘误
doi：10.1007/s00441-018-2865-3
更新日期：2018-10-01 00:00:00
abstract：:Adrenomedullin (AM) is a peptide hormone involved in the modulation of cellular growth, migration, apoptosis, and angiogenesis. These characteristics suggest that AM is involved in the control of neural stem/progenitor cell (NSPC) biology. To explore this hypothesis, we have obtained NSPC from the olfactory bulb of ad...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-010-0934-3
更新日期：2010-04-01 00:00:00
abstract：:Young rats were injected with 3H-fucose and killed at 10 min, 1, 4 and 24 h thereafter. Samples of the pancreas were processed for light- and electron-microscopic radioautography and then analyzed quantitatively. Fucose label was taken up in the supranuclear region of acinar pancreatic cells that were maximally labele...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222243
更新日期：1983-01-01 00:00:00
abstract：:The formaldehyde-induced fluorescence technique had shown 5-hydroxytryptamine-containing enteric neurons in the intestine of the teleost Platycephalus bassensis, but did not reveal such neurons in the intestine of Tetractenos glaber or Anguilla australis. Re-examination of these animals with 5-hydroxytryptamine immuno...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00226505
更新日期：1988-01-01 00:00:00
abstract：:The neurotensin-cell is identified immunohistochemically and ultrastructurally by differential counting of endocrine cells in the gut of a primate (Tupaia belangeri). Utilizing light microscopy, the EC-cells are identified by the Masson-Fontana silver stain; with the same method the neurotensin cells are not stained. ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220968
更新日期：1977-11-23 00:00:00
abstract：:The lymphatic organization and its relationship to the vascular system in the rat small intestine was studied by scanning electron microscopy of corrosion casts and freeze-fractured tissues, and by light microscopy of injected preparations. The villus possessed 3-10 or more central lacteals depending upon the villous ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218204
更新日期：1987-05-01 00:00:00
abstract：:The LIM homeodomain transcription factor Lmx1a shows a dynamic expression in the developing mouse ear that stabilizes in the non-sensory epithelium. Previous work showed that Lmx1a functional null mutants have an additional sensory hair cell patch in the posterior wall of a cochlear duct and have a mix of vestibular a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-03163-y
更新日期：2020-06-01 00:00:00
abstract：:This study aimed to characterize porcine Achilles tendon (PAT) in terms of its structural components, vascularity, and resident tendon cells. We found that PAT is composed of a paratenon sheath, a core of fascicles, and an endotenon/interfascicular matrix (IFM) that encases the fascicle bundles. We analyzed each of th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03379-3
更新日期：2021-01-26 00:00:00
abstract：:Since their introduction to biological imaging, quantum dots (QDs) have progressed from a little known, but attractive, technology to one that has gained broad application in many areas of biology. The versatile properties of these fluorescent nanoparticles have allowed investigators to conduct biological studies with...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-014-2087-2
更新日期：2015-04-01 00:00:00
abstract：:We chose synaptic terminals of rat rod bipolar cells as a model system to study activity-related changes in the overall morphology and the fine structure of synaptic sites. Using confocal laser scanning microscopy in conjunction with three-dimensional reconstruction and electron microscopy, we examined the effect of l...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051174
更新日期：1998-11-01 00:00:00
abstract：:Maternal immunoglobulin-G (IgG) is known to be transported across the placental syncytiotrophoblast during the period when the human fetus is incapable of manufacturing these defensive molecules. In this study we investigated the possible role of the amniochorion, that surrounds the amniotic cavity in which the fetus ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00583405
更新日期：1995-08-01 00:00:00
abstract：:Previous immunochemical investigations have demonstrated various opioid peptides in the pancreas. However, controversies exist related to the cellular localization of these peptides in the endocrine pancreas. Therefore, the guinea pig endocrine pancreas was immunohistochemically investigated for the presence of opioid...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318454
更新日期：1990-02-01 00:00:00
abstract：:A technique for organotypic in vitro culture with serum-free medium was tested for its appropriateness to mimic normal odontogenesis in the cichlid fish Hemichromis bimaculatus and the zebrafish Danio rerio. Serial semithin sections were observed by light microscopy to collect data on tooth patterning and transmission...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-1036-x
更新日期：2005-09-01 00:00:00
abstract：:Whether environmental exposure to bisphenol A (BPA) may induce reproductive disorders is still controversial but certain studies have reported that BPA may cause meiotic abnormalities in C. elegans and female mice. However, little is known about the effect of BPA on meiosis in adult males. To determine whether BPA exp...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1723-6
更新日期：2014-01-01 00:00:00
abstract：:While brain-derived neurotrophic factor (BDNF) messenger RNA (mRNA) has been localized in the developing gustatory epithelium, little information is available about neurotrophin-3 (NT-3) mRNA expression pattern in the prenatal developing gustatory and lingual epithelium. In the present study, using in situ hybridizati...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051092
更新日期：1998-06-01 00:00:00
abstract：:Semaphorin 3A (Sema3A) axon repellant serves multiple developmental functions. Sema3A mRNAs are expressed in epithelial and mesenchymal components of the developing incisor in a dynamic manner. Here, we investigate the functions of Sema3A during development of incisors using Sema3A-deficient mice. We analyze histomorp...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-1839-3
更新日期：2014-07-01 00:00:00
abstract：:The present study describes substance P-like immunoreactivity in the ciliary ganglia of monkey (Macaca fascicularis) and cat. About 60% of neurons in the monkey ciliary ganglion and 40% in the cat ciliary ganglion were substance P-like immunoreactive, ranging from faint to moderate staining. Substance P-like immunorea...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00354796
更新日期：1994-04-01 00:00:00
abstract：:The spatial localisation of insulin-like growth-factor-binding protein-2 (IGFBP-2) and its mRNA was investigated during larval and post-larval developmental stages of the gilthead seabream ( Sparus aurata) by immunohistochemistry and in situ hybridisation with specific antisera and riboprobes. During larval developmen...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0952-0
更新日期：2005-01-01 00:00:00
abstract：:The ability of levamisole (LVS) to correct the effect of thymectomy on splenic T-cell population in Bufo bufo larvae was tested. Using the nonspecific acid alpha-naphthyl acetate esterase (ANAE) technique, the T-lymphocytes displayed a characteristic reddish brown spot (T-pattern). The total number of white cells per ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00204789
更新日期：1982-01-01 00:00:00
abstract：:The occurrence and distribution of peptidergic nerves in the guinea pig uterus was studied by means of immunocytochemistry using numerous neuropeptide anti-sera. Neuropeptide Y (NPY)-immunoreactive (IR) nerves were the most abundant, whereas substance P (SP)-, calcitonin gene-related peptide (CGRP)-, and neurokinin A ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00226501
更新日期：1988-01-01 00:00:00
abstract：:The ultrastructure of the dermal papulae of a starfish (Asterias rubens) is consistent with a respiratory function. The present study has shown no regions specialized for excretory mechanisms. The papulae consist of an outer ectodermal epithelium of sensory, support and gland cells and a small basiepithelial nerve ple...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00229616
更新日期：1978-03-13 00:00:00
abstract：:Employing electron-microscopic methods that help retain polyanionic materials, we describe the extracellular coverings of a sea urchin (Lytechinus variegatus) throughout ontogeny. The surface of the embryo is covered by a two-layered cuticle (commonly called the hyaline layer), which in turn is covered by a granular l...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218028
更新日期：1985-01-01 00:00:00
abstract：:Somatostatin-like immunoreactivity was localized in nerve cell bodies and nerve terminals in the cat coeliac ganglion. Two types of somatostatin-immunoreactive cell bodies were revealed, the first being large (diameter 35 microns), numerous and weakly labelled, whereas the second was considerably smaller (diameter 10....
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327290
更新日期：1991-03-01 00:00:00
abstract：:The fine structure of one of the cellular components of the nucleus recesses lateralis of trout is described. These cells have an enormously developed smooth endoplasmic reticulum which makes their identification easy. The relationship of this cell type with the ventricular cavity, neighbouring cells and the presence ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00206343
更新日期：1982-01-01 00:00:00
abstract：:The present review describes several lines of recent evidence providing new insights into the basic principles and mechanisms of axon projection from the olfactory epithelium to the olfactory bulb. Olfactory sensory neurons are classified into approximately 1000 subtypes according to the expression of specific odorant...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410050953
更新日期：1997-11-01 00:00:00
abstract：:Duck muscle spindles show a large outer capsule enclosing a number of intrafusal muscle fibres which are individually encircled by an inner capsule. The outer capsule consists of a multilayer of 4-6 flattened cells with elongated nuclei, and are usually lined by a basement membrane. They resemble and are linked with p...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234400
更新日期：1980-01-01 00:00:00
abstract：:The extracellular matrix is involved in various morphogenetic processes which are accompanied by changes in its physicochemical properties and spatial organization. In the adult brain it contributes to cellular communication and the regulation of neuronal activity. The present study deals with the postnatal appearance...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050790
更新日期：1997-04-01 00:00:00
