Abstract：
:Since their introduction to biological imaging, quantum dots (QDs) have progressed from a little known, but attractive, technology to one that has gained broad application in many areas of biology. The versatile properties of these fluorescent nanoparticles have allowed investigators to conduct biological studies with extended spatiotemporal capabilities that were previously not possible. In this review, we focus on QD applications that provide enhanced quantitative information concerning protein dynamics and localization, including single particle tracking and immunohistochemistry, and finish by examining the prospects of upcoming applications, such as correlative light and electron microscopy and super-resolution. Advances in single molecule imaging, including multi-color and three-dimensional QD tracking, have provided new insights into the mechanisms of cell signaling and protein trafficking. New forms of QD tracking in vivo have allowed the observation of biological processes at molecular level resolution in the physiological context of the whole animal. Further methodological development of multiplexed QD-based immunohistochemistry assays should enable more quantitative analysis of key proteins in tissue samples. These advances highlight the unique quantitative data sets that QDs can provide to further our understanding of biological and disease processes.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Vu TQ,Lam WY,Hatch EW,Lidke DSdoi
10.1007/s00441-014-2087-2subject
Has Abstractpub_date
2015-04-01 00:00:00pages
71-86issue
1eissn
0302-766Xissn
1432-0878journal_volume
360pub_type
杂志文章,评审abstract：:Using isoenzyme-specific antisera, we have studied the distribution of protein kinase C isoforms in fetal mouse organs at the developmental age of 17 days. Two different sets of antibodies, produced by different manufacturers, were employed in this study. The specificity of the antisera was tested by immunoblotting ex...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318364
更新日期：1995-06-01 00:00:00
abstract：:The livers of Xenopus laevis, grouped by chronological age (0.5, 2 and 3 yrs), were studied electron microscopically. Ultrastructurally most of the melanin granules in the mature female liver showed an internal structure similar to the melanin granules of the oocytes. The hepatic melanin granules of immature females a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220293
更新日期：1977-12-19 00:00:00
abstract：:The present study attempts to elucidate the role of TRPV1 cation channel receptor on primary repair in an incision-wounded mouse cornea in vivo. Previous study revealed that blocking TRPV1 suppressed myofibroblast formation and expression of transforming growth factor β1 (TGFβ1) in cultured keratocytes or ocular fibro...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2878-y
更新日期：2018-11-01 00:00:00
abstract：:Kidneys of 2 to 10 day-old rats of Wistar and Sprague-Dawley strains were fixed with glutaraldehyde by retrograde vascular perfusion and then prepared for observation in TEM and SEM. In addition methacrylate casts of differentiating glomerular capillaries were examined by SEM. Although the glomerular vascular pattern ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233959
更新日期：1980-01-01 00:00:00
abstract：:Coincident with an increase in the water permeability of toad urinary bladder induced by serosal hypertonicity, a transformation of the ridge-like surface structures of the granular cells into individual microvillous structures occurs. This study was initiated to establish whether the transformation is mediated by the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217226
更新日期：1984-01-01 00:00:00
abstract：:Electron-microscopic evidence indicates that during conditions of high hormone demand such as lactation there is a dramatic reduction in the number of fine glial processes which are normally interposed between magnocellular neuroendocrine cell somata in the supraoptic nucleus (SON). The purpose of this study was to co...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00225557
更新日期：1985-01-01 00:00:00
abstract：:In this immunocytochemical study we investigated the distribution of nervous structures in the lower lip of adult rats. The region is characterized by a rich cutaneous and mucosal sensory innervation originating from terminal branches of the trigeminal system. Lower lip innervation was investigated by detection of the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051348
更新日期：1999-08-01 00:00:00
abstract：:The distribution and nature of 125I-atrial natriuretic peptide binding sites have been examined in the brain and pituitary gland of the toad, Bufo marinus, using tissue section autoradiography, affinity cross-linking and electrophoresis, guanylyl cyclase assays and molecular analysis of natriuretic peptide receptor C ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000266
更新日期：2000-10-01 00:00:00
abstract：:Although the cerebrospinal fluid-contacting neurons of the avian paraventricular organ exhibit considerable amounts of catecholamines, they show no tyrosine hydroxylase immunoreactivity. In the quail embryo, the development of these neurons has been studied using the paraformaldeyde-glutaraldeyde method for the fluore...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00307970
更新日期：1995-07-01 00:00:00
abstract：:By a regular immunoperoxidase method, alpha-1-proteinase inhibitor (Api) was demonstrated in pancreatic islets in individuals with and without genetic deficiency of Api. Subsequently a double immunoperoxidase method, with two different chromogens (diaminobenzidine-brown and 4-chloro-1-naphthol-blue), was applied on th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218078
更新日期：1986-01-01 00:00:00
abstract：:The postnatal maturation of regions of the epididymis and intragonadal segment of the deferens duct was studied in the rat by light- and transmission electron microscopy. Maturation of the genital duct starts in the distal cauda epididymidis and ductus deferens after one week of life, and one week later, in the more c...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215508
更新日期：1987-08-01 00:00:00
abstract：:Gravid females of the parasitoid fly species Ormia ochracea (Diptera: Tachinidae) rely on their sense of hearing to detect and localize their hosts, field crickets of the genus Gryllus. As in any hearing insect species, the fly's auditory system consists of a rather complex cascade of mechanical and physiological mech...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000257
更新日期：2000-09-01 00:00:00
abstract：:We used immunohistochemistry to identify cells present in pituitary allografts in the hamster. Hypophyses removed from neonatal hamsters or adenohypophyses removed from adult females were placed beneath renal capsules of hypophysectomized adult females. Serum PRL, LH, and GH concentrations were measured at two, five a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215467
更新日期：1988-01-01 00:00:00
abstract：:Antithrombin (AT), which is mainly synthesized in the liver, is an acute-phase plasma protein in mammalian species. Here, we demonstrated that sheep anti-human AT antibody cross-reacted with the humoral fluids in amphioxus Branchiostoma belcheri tsingtauense as well as human serum. The concentration of AT in the humor...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0088-x
更新日期：2006-03-01 00:00:00
abstract：:This study aimed to characterize porcine Achilles tendon (PAT) in terms of its structural components, vascularity, and resident tendon cells. We found that PAT is composed of a paratenon sheath, a core of fascicles, and an endotenon/interfascicular matrix (IFM) that encases the fascicle bundles. We analyzed each of th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03379-3
更新日期：2021-01-26 00:00:00
abstract：:The organization of the system of perivascular space around the capillaries in the neurohypophysis was studied in the adult and developing laboratory mouse by the use of histological silver impregnation and electron microscopical techniques. In the median eminence short and long extensions, arising mainly from the sha...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00237730
更新日期：1979-06-08 00:00:00
abstract：:Boar spermadhesin (AWN) is a 14-kDa multifunctional protein, attached to the surface of the spermatozoa and involved in sperm capacitation and zona pellucida binding. The cellular origin of AWN was previously unknown. Moreover, the region of the male genital tract in which AWN becomes attached to the surface of sperma...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00319144
更新日期：1995-10-01 00:00:00
abstract：:Cytochemical methods for the demonstration of p-nitrophenylphosphatase (p-NPPase) at the electron-microscopic level were applied to avian osteoclasts to elucidate some of the functional differences among ruffled border, other membrane systems, and cellular organelles. The localization of p-NPPase activity occurred in ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218381
更新日期：1986-01-01 00:00:00
abstract：:Type 1 diabetes is an autoimmune disorder characterized by a lack of insulin production by the beta cells of the pancreas. This lack of insulin causes a variety of systemic effects on whole-body metabolism. Poorly managed type 1 diabetes can lead to cardiovascular disease, diabetic neuropathy, and diabetic retinopathy...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-012-1387-7
更新日期：2012-09-01 00:00:00
abstract：:The amniotic epithelium constitutes a diffusion barrier controlling the passage of solutes and water between the amniotic cavity and maternal circulation. With the present immunocytochemical approach, we have shown that several major components of the cyto-skeleton, i.e., actin, alpha-actinin, spectrin and ezrin, are ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318194
更新日期：1991-11-01 00:00:00
abstract：:Calcitonin gene-related peptide (CGRP) was found extensively in the small intestine of both non-mammalian and mammalian vertebrates using radioimmunoassay and immunocytochemistry. By radioimmunoassay, the levels of CGRP in rats, mice, chickens, bullfrogs and rainbow trout were found to range from 91.5 to 419.1 ng/g ti...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00223142
更新日期：1989-10-01 00:00:00
abstract：:Neural lobes of control and "Jimpy" mice were examined electron microscopically. The ultrastructure and incidence of pituicytes was examined following reports of reductions in astrocyte and oligodendrocyte populations in areas of the CNS of Jimpy mice. The failure to demonstrate any modification of structure or number...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220162
更新日期：1976-03-05 00:00:00
abstract：:The dorsal root ganglion (DRG) and dorsal horn of the spinal cord are areas through which primary afferent information passes enroute to the brain. Previous studies have reported that, during normal neuronal activity, the regional distribution of a second messenger, diacylglycerol (DG), which is derived from phosphoin...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0219-z
更新日期：2006-10-01 00:00:00
abstract：:The antioxidant enzymes superoxide dismutase, glutathione peroxidase, and catalase were measured in the rete mirabile and gas gland epithelium area of the swim bladder of the toadfish Opsanus tau. When the concentration of enzyme in the swim bladder was compared with the concentration in other organs (kidney, heart, g...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210458
更新日期：1981-01-01 00:00:00
abstract：:Numerous nerve fibres containing acetylcholinesterase and noradrenaline, as well as avian pancreatic polypeptide-, vasoactive intestinal peptide-, or substance P-like immunoreactivity are observed around arteries in the external carotid rete of the cat. The nerves are located in the adventitial layer close to the medi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218360
更新日期：1982-01-01 00:00:00
abstract：:In the central nervous system, serotonergic and dopaminergic signaling is terminated by the activity of specialized transporter proteins for serotonin (SERT) and dopamine (DAT). These transporter proteins are found both on the cell surface and in intracellular transport vesicles. Trafficking between these subcellular ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1256-9
更新日期：2011-11-01 00:00:00
abstract：:Back-labeling of regenerated electromotor neurons in the teleost Sternarchus albifrons was performed to test the hypothesis that, in regenerated spinal cord, incorrectly located electromotor neurons are eliminated because their axons do not reach the correct target area (electric organ). In each cross section examined...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217166
更新日期：1985-01-01 00:00:00
abstract：:Antibodies to smooth muscle and non-muscle myosin allow the development of smooth muscle and its capillary system in the embryonic chicken gizzard to be followed by immunofluorescent techniques. Although smooth muscle development proceeds in a serosal to luminal direction, angiogenetic cell clusters develop independen...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00306123
更新日期：1994-05-01 00:00:00
abstract：:Ionizing irradiation is an effective treatment for intracranial tumors but is limited by the potential adverse effects induced in surrounding normal brain. These effects can include cognitive impairments, and whereas the pathogenesis of such injury has not yet been definitively established, it may involve injury to th...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-007-0480-9
更新日期：2008-01-01 00:00:00
abstract：:The localization of inhibin alpha-subunit in the human testis was studied at the light- and electron-microscope level with immunostaining techniques. Antibodies against specific fragments of porcine and human inhibin alpha-subunits were utilized. At light microscopy, inhibin alpha-subunit immunoreactivity was detected...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00319612
更新日期：1992-08-01 00:00:00
