Abstract：
:Previous immunochemical investigations have demonstrated various opioid peptides in the pancreas. However, controversies exist related to the cellular localization of these peptides in the endocrine pancreas. Therefore, the guinea pig endocrine pancreas was immunohistochemically investigated for the presence of opioid peptides derived from pro-dynorphin, pro-enkephalin or pro-opiomelano-cortin. Immunoreactivities were demonstrated on serial semithin sections by the peroxidase anti-peroxidase technique. In routinely immunostained sections, immunoreactivities for dynorphin A and alpha-neo-endorphin were localized in pancreatic enterochromaffin cells, but not in islet cells. Immunoreactivity for Met-enkephalin was confined exclusively to B-cells and was localized only in some secretory granules. However, pre-treatment of semi-thin sections with trypsin and carboxypeptidase B led to a marked increase of Met-enkephalin immunoreactivity in B-cells. In addition, immunoreactivities for Met-enkephalin-Arg-Gly-Leu and bovine adrenal medulla dodecapeptide could be demonstrated in B- and A-cells, and beta-endorphin immunoreactivity was localized in A-cells. In no case, however, were immunoreactivities detected for bovine adrenal medulla docosapeptide, peptide F, corticotropin, melanotropin or dynorphin 1-32. The immunohistochemical findings indicate that opioids of different peptide families are present in the guinea pig endocrine pancreas. Since several opioid peptides of the corresponding pro-hormones could be demonstrated in the reference organs but not in the pancreas, it is concluded that the biosynthetic pathways of the respective precursors are different from those in the adrenal medulla or in the pituitary.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Cetin Ydoi
10.1007/BF00318454subject
Has Abstractpub_date
1990-02-01 00:00:00pages
313-9issue
2eissn
0302-766Xissn
1432-0878journal_volume
259pub_type
杂志文章abstract：:In the present study, we characterized intra-epithelial leukocytes in the digestive tract of chickens during postnatal development. Their phenotype was characterized by monoclonal antibodies in cryostat sections and the numbers of the different cell-types were counted in the epithelium of the esophagus, proventriculus...
journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0200-x
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000342
更新日期：2001-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0024-0
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209963
更新日期：1981-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216347
更新日期：1985-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0004-4
更新日期：2005-11-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 临床试验,杂志文章
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215830
更新日期：1988-02-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234184
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2012-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
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更新日期：2006-11-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1977-10-26 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1978-01-17 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215882
更新日期：1976-05-06 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1994-05-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213851
更新日期：1982-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1517-2
更新日期：2013-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00224328
更新日期：1976-03-16 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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