Abstract：
:Induction of HIF-1α by oxygen limitation promotes increased phosphorylation and catalytic depression of mitochondrial pyruvate dehydrogenase (PDH) and an enhanced glycolytic poise in cells. Cobalt chloride and desferrioxamine are widely used as mimics for hypoxia because they increase the levels of HIF-1α. We evaluated the ability of these agents to elicit selected physiological responses to hypoxia as a means to metabolically precondition mammalian cells, but without the detrimental effects of hypoxia. We show that, while CoCl(2) does increase HIF-1α in a dose-dependent manner, it unexpectedly and strikingly decreases PDH phosphorylation at E1α sites 1, 2, and 3 (Ser(293), Ser(300), and Ser(232), respectively) in HepG2 cells. This same effect is also observed for site 1 in mouse NIH/3T3 fibroblasts and J774 macrophages. CoCl(2) unexpectedly decreases the mRNA expression for PDH kinase-2 in HepG2 cells, which likely explains the dephosphorylation of PDH observed. And nor does desferrioxamine promote the expected increase in PDH phosphorylation. Dimethyloxaloylglycine (a prolyl hydroxylase inhibitor) performs better in this regard, but failed to promote the stronger effects seen with hypoxia. Consequently, CoCl(2) and desferrioxamine are unreliable mimics of hypoxia for physiological events downstream of HIF-1α stabilization. Our study demonstrates that mimetic chemicals must be chosen with caution and evaluated thoroughly if bona fide cellular outcomes are to be promoted with fidelity.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Borcar A,Menze MA,Toner M,Hand SCdoi
10.1007/s00441-012-1517-2subject
Has Abstractpub_date
2013-01-01 00:00:00pages
99-106issue
1eissn
0302-766Xissn
1432-0878journal_volume
351pub_type
杂志文章abstract：:The organization of the neuropeptide Y (NPY)-immunoreactive system in the forebrain, pineal organ and retina of a biweekly spawning fish (Fundulus heteroclitus) was investigated. Immunoreactivity was encountered in neurons of the nucleus olfactoretinalis, in the large population of neurons in the floor of the telencep...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050541
更新日期：1996-02-01 00:00:00
abstract：:iASPP (inhibitory member of the ASPP family), the conserved key inhibitor of p53, is overexpressed and plays an important oncogenetic role in many human cancers. However, its function in cervical cancer remains unknown. The objective of this study was to investigate the expression and clinical relevance of iASPP in ce...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1569-y
更新日期：2013-05-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00226501
更新日期：1988-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217235
更新日期：1984-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：1987-07-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2001-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0979-2
更新日期：2005-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00239453
更新日期：1989-11-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1477-6
更新日期：2012-11-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004419900071
更新日期：1999-10-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050641
更新日期：1996-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-010-1049-6
更新日期：2011-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0188-2
更新日期：2006-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF01740771
更新日期：1990-03-01 00:00:00
abstract：:The effects of the administration of thyroxine (T4) on the postnatal cytodifferentiation of granular convoluted tubule (GCT) cells of the submandibular gland (SMG) of Lewiss-Webster mice were studied by light and electron microscopy. From birth, mice of both sexes were injected daily with T4 (sc 0.4 micrograms/g BW) a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218202
更新日期：1987-05-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217273
更新日期：1982-01-01 00:00:00
abstract：:Early in postnatal life, the first wave of spermatogenesis is accompanied by an initial wave of germ cell apoptosis. This may reflect an adjustment in the number of germ cells that can be adequately maintained by Sertoli cells. Two major pathways (intrinsic and extrinsic) are involved in the process of caspase activat...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0186-4
更新日期：2006-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-014-2029-z
更新日期：2015-06-01 00:00:00
abstract：:The rejection of skin allografts by the larval lamprey, Lampetra reissneri, was studied by light- and electron-microscopy, with particular attention to the cell types involved in the reaction. In all allografts, melanophores were destroyed within 20-60 days (the mean survival time, 36 +/- 12 days). Neither the epiderm...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222182
更新日期：1983-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218715
更新日期：1976-12-10 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215908
更新日期：1986-01-01 00:00:00
abstract：:Appearance of immunoreactive corticotropin-releasing factor (CRF)-containing neurons was studied in developing hypothalamus of the rat by use of antisera against rat- and ovine CRF. These neurons were first recognized in the lateral and paraventricular nuclei on days 15.5 and 16.5 of gestation, respectively, when anti...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219870
更新日期：1984-01-01 00:00:00
abstract：:The blood-brain barrier (BBB) protects the brain from toxic substances within the bloodstream and keeps the brain's homeostasis stable. On the other hand, it also represents the main obstacle in the treatment of many CNS diseases. Among different techniques, nanoparticles have emerged as promising tools to enhance bra...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
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更新日期：2014-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209040
更新日期：1978-10-17 00:00:00
abstract：:Electron microscopy of the rat lymph node disclosed reticular cells in close association with bundles of collagenous fibrils 30-45 nm in diameter, and electron lucent "amorphous" substances surrounded by microfibrils 11 nm in diameter, with junctional complexes. The amorphous substances, distinctly revealed by orcein ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210522
更新日期：1981-01-01 00:00:00
abstract：:It is clear that the well-described phenomenon of epithelial-mesenchymal transition (EMT) plays a pivotal role in embryonic development, wound healing, tissue regeneration, organ fibrosis and cancer progression. EMTs have been classified into three subtypes based on the functional consequences and biomarker context in...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-011-1227-1
更新日期：2012-01-01 00:00:00
abstract：:Comparing the distribution of cytoplasmic particles and organelles between different experimental conditions can be challenging due to the heterogeneous nature of cell morphologies. The border-to-border distribution method was created to enable the quantitative analysis of fluorescently labeled cytoplasmic particles a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-015-2265-x
更新日期：2016-02-01 00:00:00
abstract：:The olfactory epithelium of mice after axotomy was investigated to clarify the stem cells of olfactory cells by double immunostaining using antikeratin (MA903) and anti-bromodeoxyuridine (BrdU) antibodies and by conventional electron microscopy. When a single dose of BrdU was given to mice 9 days after axotomy, immuno...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318179
更新日期：1991-11-01 00:00:00
