Colocalization of oxidized low-density lipoprotein, caspase-3, cyclooxygenase-2, and macrophage migration inhibitory factor in arteriosclerotic human carotid arteries.

Abstract:

:Apoptotic and inflammatory processes occur in human arteriosclerotic lesions. We examined the hypothesis whether both processes are possibly associated by studying the colocalization of corresponding markers. In 11 human arteriosclerotic carotid arteries, proapoptotic markers (CPP32 (caspase-3), poly(ADP-ribose) polymerase, apoptosis-inducing factor, c-Jun/AP-1, and p53) and proinflammatory markers (macrophage migration inhibitory factor (MIF) and cyclooxygenase-2) were found in macrophages (MPhi) evaluated by computer-assisted immunohistomorphometry. Double-labeling studies demonstrated a colocalization of, both, proapoptotic and proinflammatory markers in these MPhi. Moreover, these MPhi also contained oxidized low-density lipoproteins (oxLDL). Exposure of cultured human MPhi to oxLDL, C6-ceramide, and tumor necrosis factor-alpha or H2O2 resulted in a significant increase of the apoptosis rate as well as of the MIF protein expression. Our study of MPhi in arteriosclerotic carotid arteries and in vitro experiments provide evidence that markers of apoptosis and inflammation are not only significantly increased but are also coexpressed. We conclude there are reciprocal modulatory interactions between apoptotic and inflammatory pathways in human plaque MPhi, which might importantly modify plaque progression or stability.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Schlittenhardt D,Schmiedt W,Bonaterra GA,Metz J,Kinscherf R

doi

10.1007/s00441-005-0024-0

keywords:

subject

Has Abstract

pub_date

2005-12-01 00:00:00

pages

425-35

issue

3

eissn

0302-766X

issn

1432-0878

journal_volume

322

pub_type

杂志文章
  • Nerve fibers immunoreactive for substance P and calcitonin gene-related peptide in the cervical spinal ventral roots of the mouse.

    abstract::We demonstrate the existence of nerve fibers possessing substance P (SP) and calcitonin gene-related peptide (CGRP) immunoreactivity in the mouse cervical ventral roots. The distribution of the SP and CGRP fibers was similar, but CGRP fibers were generally more numerous. Both types entered the ventral pia mater or for...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00327774

    authors: Kimura M,Kishida R,Abe T,Goris RC,Kawai S

    更新日期:1994-08-01 00:00:00

  • Enterochromaffin (EC-) cells of the mammalian gastro-entero-pancreatic (GEP) endocrine system: cellular source of pro-dynorphin-derived peptides.

    abstract::It has long been disputed whether mammalian enterochromaffin (EC-) cells contain a peptide in addition to serotonin. Previous immunohistochemical studies have provided evidence for the presence of enkephalins in EC-cells. These findings, however, are equivocal. Therefore, the problem of opioid peptides in EC-cells has...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00221752

    authors: Cetin Y

    更新日期:1988-07-01 00:00:00

  • Subsurface cisterns and lamellar bodies in the granule cells of the guinea-pig fascia dentata.

    abstract::Subsurface cisterns (SSC's) and less frequent lamellar bodies (LB's) were identified in the granule cells of the guinea-pig fascia dentata. Both structures, composed of flattened or collapsed agranular cisterns, are continuous with the regular rough endoplasmic reticulum and occasionally connected with each other form...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF01258502

    authors: Kolde G,Themann H

    更新日期:1982-01-01 00:00:00

  • Two-color double-labeling in situ hybridization of whole-mount Hydra using RNA probes for five different Hydra neuropeptide preprohormones: evidence for colocalization.

    abstract::The freshwater polyp Hydra magnipapillata has a primitive nervous system that produces at least three distinct classes of neuropeptides: various peptides having the C-terminal sequence Arg-Phe-NH2 (the Hydra-RFamide family), Leu-Trp-NH2 (the Hydra-LWamide family), and a single peptide having the C-terminal sequence Ly...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410000240

    authors: Hansen GN,Williamson M,Grimmelikhuijzen CJ

    更新日期:2000-08-01 00:00:00

  • Antral G-cell in gastrin and gastrin-cholecystokinin knockout animals.

    abstract::The antral hormone gastrin is the key regulator of gastric acid secretion, mucosal growth and differentiation. Gastrin is synthesized in the endocrine G-cells in the antroduodenal mucosa. We have now examined the way in which the loss of gastrin alone or gastrin plus cholecystokinin (CCK) affects the antral G-cell. Im...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-005-1110-z

    authors: Friis-Hansen L,Wierup N,Rehfeld JF,Sundler F

    更新日期:2005-07-01 00:00:00

  • Neurofilament dynamics and involvement in neurological disorders.

    abstract::Neurons are extremely polarised cells in which the cytoskeleton, composed of microtubules, microfilaments and neurofilaments, plays a crucial role in maintaining structure and function. Neurofilaments, the 10-nm intermediate filaments of neurons, provide structure and mechanoresistance but also provide a scaffolding f...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s00441-014-2082-7

    authors: Gentil BJ,Tibshirani M,Durham HD

    更新日期:2015-06-01 00:00:00

  • β Integrin-like protein-mediated adhesion and its disturbances during cell cultivation of the mussel Mytilus trossulus.

    abstract::In this study, we focus on the specific contribution of β integrin-like protein to adhesion-mediated events in molluscan larval cells in culture that could not have been investigated within the whole animal. An analysis of disturbances to cell-substratum adhesion, caused by the integrin receptor inhibiting Arg-Gly-Asp...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-015-2122-y

    authors: Maiorova MA,Odintsova NA

    更新日期:2015-08-01 00:00:00

  • Immunohistochemical study of 5-HT-containing neurons in the teleost intestine: relationship to the presence of enterochromaffin cells.

    abstract::The formaldehyde-induced fluorescence technique had shown 5-hydroxytryptamine-containing enteric neurons in the intestine of the teleost Platycephalus bassensis, but did not reveal such neurons in the intestine of Tetractenos glaber or Anguilla australis. Re-examination of these animals with 5-hydroxytryptamine immuno...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00226505

    authors: Anderson C,Campbell G

    更新日期:1988-01-01 00:00:00

  • Thyroid hormone activates Wnt/β-catenin signaling involved in adult epithelial development during intestinal remodeling in Xenopus laevis.

    abstract::During amphibian intestinal remodeling, thyroid hormone (TH) induces some larval epithelial cells to dedifferentiate into adult stem cells, which newly generate the absorptive epithelium analogous to the mammalian epithelium. To clarify molecular mechanisms underlying adult epithelial development, we here focus on TH ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-016-2396-8

    authors: Hasebe T,Fujimoto K,Kajita M,Ishizuya-Oka A

    更新日期:2016-08-01 00:00:00

  • Electron-cytochemical localization of alkaline phosphatase to G cells of Necturus maculosus antrum.

    abstract::Electron-cytochemical localization of alkaline phosphatase activity was performed on G cells of Necturus maculosus antral mucosa. Alkaline phosphatase activity was localized to the nuclear membrane, the Golgi/endoplasmic reticulum, and the limiting membranes of G cell peptide-secretion vesicles. There was no specific ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00218867

    authors: Rutten MJ,Garrison RG,Moore CD,Fiskin AM,Cheung LY

    更新日期:1989-12-01 00:00:00

  • Architecture of associations of minor salivary gland ducts and lymphoid follicles in Macaca fascicularis. An ultrastructural study.

    abstract::Previous light-microscopic and ultra-immunohistochemical tracer studies revealed the existence of an independent local immune response of the simian oral mucosa. This local response is attributed to the presence of minor salivary gland (MSG) duct-related lymphoid tissue. Semithin sections from a total of 263 Epon-embe...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00217578

    authors: Nair PN,Schroeder HE

    更新日期:1985-01-01 00:00:00

  • Feeding-induced changes of melanin-concentrating hormone (MCH)-like immunoreactivity in goldfish brain.

    abstract::Intracerebroventricular (ICV) injection of melanin-concentrating hormone (MCH) influences feeding behavior in the goldfish and exerts an anorexigenic action in goldfish brain, unlike its orexigenic action in mammals. Despite a growing body of knowledge concerning MCH function in mammals, the role of MCH in appetite ha...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-006-0347-5

    authors: Matsuda K,Shimakura S,Miura T,Maruyama K,Uchiyama M,Kawauchi H,Shioda S,Takahashi A

    更新日期:2007-05-01 00:00:00

  • Significance of the peri-insular extracellular matrix for islet isolation from the pancreas of rat, dog, pig, and man.

    abstract::The presence and distribution in the peri-insular region of extracellular matrix, and in particular basement membrane, was investigated in a comparative study comprising pancreata of rat, dog, pig, and man. Basement membrane markers, collagen type-IV and laminin, were determined immunohistochemically. Additional infor...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00318700

    authors: van Deijnen JH,Hulstaert CE,Wolters GH,van Schilfgaarde R

    更新日期:1992-01-01 00:00:00

  • Ultrastructural changes in the calcium-sensitive (PAS-positive) cells of the pars intermedia of eels kept in deionized water and in normal and concentrated sea water.

    abstract::The ultrastructure of the calcium-sensitive (Ca-s) (PAS-positive) cells of the pars intermedia was investigated in eels kept in hypo- and hyperosmotic environments. Although the cells were moderately active in fresh water (FW), they were highly stimulated in deionized water (DW) and displayed an enlarged Golgi apparat...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00210015

    authors: Olivereau M,Chambolle P,Dubourg P

    更新日期:1981-01-01 00:00:00

  • Ultrastructure of the nongranulated cells and morphology of the extracellular spaces in the pars distalis of adult and pouch-young tammar wallabies (Macropus eugenii).

    abstract::Differentiated nongranulated (folliculo-stellate) cells were observed in the centre and periphery of the cords of cells in the pars distalis of the adult tammar wallaby (Macropus eugenii). The nongranulated cells formed follicles containing a small lumen: the cell apices were joined by junctional complexes including z...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00210898

    authors: Leatherland JF,Renfree MB

    更新日期:1982-01-01 00:00:00

  • Fate of annular gap junctions in the papillary cells of the enamel organ in the rat incisor.

    abstract::To investigate the mechanisms whereby annular gap junctions in the papillary cells of the enamel organ are degraded intracellularly, continuously growing rat incisors were examined by electron microscopy of routine thin sections as well as for the cytochemical localization of inorganic trimetaphosphatase activity. Rou...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00215192

    authors: Sasaki T,Garant PR

    更新日期:1986-01-01 00:00:00

  • Tissue- and species-specific expression patterns of class I, III, and IV Adh and Aldh 1 mRNAs in rodent embryos.

    abstract::Alcohol and aldehyde dehydrogenases (ADHs and ALDHs) may be of interest in the pathology of Parkinson's disease (PD) because of their role in protection against toxins and in retinoid metabolism, which is required for growth and development of the mesencephalic dopamine system. In the present study, the spatial and te...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-005-0038-7

    authors: Westerlund M,Galter D,Carmine A,Olson L

    更新日期:2005-11-01 00:00:00

  • Heterologous expression of acetylcholinesterase affects proliferation and glial cytoskeleton of adherent chicken retinal cells.

    abstract::Besides its function at cholinergic synapses, acetylcholinesterase (AChE) exerts structural functions on neural differentiation, independent of its enzymatic activity. To elucidate such functions, we have previously heterologously expressed AChE in histotypic retinal reaggregates, revealing strong effects on their his...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410100444

    authors: Keller M,Robitzki A,Layer PG

    更新日期:2001-11-01 00:00:00

  • Daily fluctuations of nucleoli in neurosecretory cells of the rat supraoptic nucleus. An ultrastructural and stereological study.

    abstract::Daily fluctuations of nucleoli and nucleolar fibrillar centres in neurosecretory cells from the supraoptic nucleus (SON) were investigated in rats artificially synchronized for 3 weeks to a set 12 h light/12 h dark cycle with free access to food and water. Group of 3 animals were sacrificed by intracardiac perfusion e...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00222351

    authors: Bessone R,Seïte R

    更新日期:1985-01-01 00:00:00

  • Effects of growth rate and cell density on nerve growth factor secretion in cultures of vascular and bladder smooth muscle cells from hypertensive and hyperactive rats.

    abstract::Elevated target-derived smooth muscle nerve growth factor (NGF) and resultant neurogenic plasticity are associated with both hypertension and hyperactive voiding in spontaneously hypertensive rats (SHRs: hypertensive, behaviorally hyperactive). In culture, vascular (VSMCs) and bladder (BSMCs) smooth muscle cells deriv...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410051194

    authors: Clemow DB,Tuttle JB

    更新日期:1998-12-01 00:00:00

  • Immune-complex-trapping cells in the spleen of the oriental fire-bellied toad, Bombina orientalis.

    abstract::The spleen of the oriental fire-bellied toad, Bombina orientalis, consists of well-developed white pulp, separated from the lymphocytic marginal zone by the connective tissue boundary layer. Injection of peroxidase-conjugated rabbit anti-peroxidase revealed that these immune complexes were localized on the surface of ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00297553

    authors: Dulak J,Bauer J,Döpp EA,Van Rooijen N

    更新日期:1993-01-01 00:00:00

  • Effects of colchicine on the ultrastructure of mouse taste buds.

    abstract::Effect of colchicine on the ultrastructure of taste bud cells was studied in the mouse. In untreated mice microtubules were abundant throughout the entire cytoplasm of type-III cells, but only in the apical cytoplasm of type-I cells. After 2 h of colchicine treatment, no microtubules were observed in any taste bud cel...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00214555

    authors: Takeda M,Suzuki Y,Shishido Y

    更新日期:1985-01-01 00:00:00

  • Dominance of parental genomes in embryonic stem cell/fibroblast hybrid cells depends on the ploidy of the somatic partner.

    abstract::Two dozen hybrid clones were produced by fusion of diploid embryonic stem (ES) cells positive for green fluorescent protein (GFP) with tetraploid fibroblasts derived from DD/c and C57BL-I(I)1RK mice. Cytogenetic analysis demonstrated that most cells from these hybrid clones contained near-hexaploid chromosome sets. Ad...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-010-0987-3

    authors: Kruglova AA,Matveeva NM,Gridina MM,Battulin NR,Karpov A,Kiseleva EV,Morozova KN,Serov OL

    更新日期:2010-06-01 00:00:00

  • Rab11, a vesicular trafficking protein, affects endoreplication through Ras-mediated pathway in Drosophila melanogaster.

    abstract::Rab11, a small monomeric GTPase associated with recycling endosomes, is a key molecule in the regulation of vesicular trafficking and is involved in the development and differentiation of many Drosophila tissues through interaction with diverse signaling pathways. In this study, we report for the first time that Rab11...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-016-2500-0

    authors: Choubey PK,Roy JK

    更新日期:2017-02-01 00:00:00

  • Ultrastructural and immunohistochemical studies of microfibril-associated components in the posterior chamber of the eye.

    abstract::Connective tissue microfibrils were observed in tissues prepared with methods believed to minimize the loss of tissue components. The eyes of C57BL/6J mice were fixed with glutaraldehyde followed by either freeze substitution, or embedding in glycol methacrylate, a water-miscible embedding medium, after limited or no ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00318486

    authors: Inoue S

    更新日期:1995-02-01 00:00:00

  • Remodeling of the notochord during development of vertebral fusions in Atlantic salmon (Salmo salar).

    abstract::Histological characterization of spinal fusions in Atlantic salmon (Salmo salar) has demonstrated shape alterations of vertebral body endplates, a reduced intervertebral space, and replacement of intervertebral cells by ectopic bone. However, the significance of the notochord during the fusion process has not been add...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-010-1069-2

    authors: Ytteborg E,Torgersen JS,Pedersen ME,Baeverfjord G,Hannesson KO,Takle H

    更新日期:2010-12-01 00:00:00

  • Effects of bunaftine on morphology, microfilament integrity, and mitotic activity in cultured human fibroblasts and HeLa cells.

    abstract::Human fibroblasts and HeLa cells were treated with bunaftine (N-butyl-N-/2-(diethylamino)ethyl/-1-naphthalenecarboxamide ) in vitro. At concentrations of 0.5-2.0 mM, the drug caused contraction and rounding of the cells with loss of microvilli-like processes. Aggregates of dense, partly granular, partly fibrillar mate...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00216519

    authors: Moskalewski S,Thyberg J

    更新日期:1984-01-01 00:00:00

  • Light and electron microscopic studies on the excretory system of Macrobiotus richtersi Murray, 1911 (Eutardigrada).

    abstract::The excretory system of Macrobiotus richtersi consists of one dorsal and two lateral components and shows a high degree of structural complexity. In each of these a tricellular external lobe and a column can be distinguished, the two parts being connected distally. The surface of the lobe cells is increased by deep ba...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00239339

    authors: Weglarska B

    更新日期:1980-01-01 00:00:00

  • Silicatein-mediated incorporation of titanium into spicules from the demosponge Suberites domuncula.

    abstract::Primmorphs (a three-dimensional sponge primary cell culture system) have been revealed to be a cell/tissue nano-factory for the production of tailor-made hybrid nanostructures. Growth of primmorphs is stimulated by the presence of a titanium alkoxide precursor tolerating titania (TiO2) concentrations up to 250 microM....

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-009-0903-x

    authors: Natalio F,Mugnaioli E,Wiens M,Wang X,Schröder HC,Tahir MN,Tremel W,Kolb U,Müller WE

    更新日期:2010-02-01 00:00:00

  • Immunohistochemical localization of serotonin in intrapulmonary neuro-epithelial bodies.

    abstract::A recently developed immunohistochemical technique for serotonin (Steinbusch et al. 1978) was used in the present investigation to study the occurrence of this indoleamine in the granulated epithelial cells of neuro-epithelial bodies (NEB). Lungs from neonatal rabbits and pigs exhibit immunoreactive cell groups identi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00217095

    authors: Lauweryns JM,de Bock V,Verhofstad AA,Steinbusch HW

    更新日期:1982-01-01 00:00:00