Abstract：
:The presence and distribution in the peri-insular region of extracellular matrix, and in particular basement membrane, was investigated in a comparative study comprising pancreata of rat, dog, pig, and man. Basement membrane markers, collagen type-IV and laminin, were determined immunohistochemically. Additional information pertaining to the structural relationships between endocrine and exocrine pancreas, in particular cell-to-cell and cell-to-matrix contacts, was obtained by electron microscopy. In pig, very little peri-insular capsule is present, and the structural integration of the porcine islet in the exocrine pancreas almost exclusively depends on cell-to-cell adhesion. In the canine pancreas, the islets are almost completely encapsulated with very little direct exocrine-to-endocrine cell-to-cell contact. In rat and man, the situation is intermediate with a tendency towards predominance of cell-to-matrix adhesion. The intra-insular adhesion mechanisms depend largely on cell-to-cell adhesion in all four species. The ultrastructural results suggest that collagenase preparations employed in islet isolation procedures should be of high purity as to preserve the protease-sensitive intra-islet cell-to-cell adhesion. Under these conditions, however, the endocrine-to-exocrine cell-to-cell contacts will be conserved also, resulting in an exocrine-tissue contamination of the islets of Langerhans. Consequently, additional steps for the effective removal of exocrine tissue and the purification of islets are required.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
van Deijnen JH,Hulstaert CE,Wolters GH,van Schilfgaarde Rdoi
10.1007/BF00318700subject
Has Abstractpub_date
1992-01-01 00:00:00pages
139-46issue
1eissn
0302-766Xissn
1432-0878journal_volume
267pub_type
杂志文章abstract：:The presence of melatonin is demonstrated in the pineal gland, the retina and the Harderian gland in some mammalian and non-mammalian vertebrates, using a specific fluorescence labelled antibody technique. Four different potent antibodies against melatonin have been used and compared. In the pineal gland of hamsters, ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233830
更新日期：1981-01-01 00:00:00
abstract：:Seven isoforms of tachykinin-related peptides (TRPs) have been isolated from the brain of the cockroach Leucophaea maderae. These peptides (LemTRP-1, 2, and 5-9) share the C-terminal sequence GFX(1)GX(2)Ramide (where X(1) and X(2) are variable residues). In order to determine the neuronal distribution of several of th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0538-7
更新日期：2002-05-01 00:00:00
abstract：:Two different fixative procedures (immersion and perfusion) and four different fixative solutions were used in order to obtain the best preservation of the brush cells of the common bile duct of the rat. The results indicate that only perfusion fixation through the common bile duct is suitable, independent of the fixa...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210353
更新日期：1981-01-01 00:00:00
abstract：:Chronological changes in renal glomerular morphology and plasma renin activity were investigated during active and hibernating periods in the golden-mantled ground squirrel Spermophilus lateralis. The objective of this study was to determine whether the glomerular endothelium, visceral epithelium (podocytes), basement...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327750
更新日期：1990-10-01 00:00:00
abstract：:The ultrastructure of the calcium-sensitive (Ca-s) (PAS-positive) cells of the pars intermedia was investigated in eels kept in hypo- and hyperosmotic environments. Although the cells were moderately active in fresh water (FW), they were highly stimulated in deionized water (DW) and displayed an enlarged Golgi apparat...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210015
更新日期：1981-01-01 00:00:00
abstract：:Triad density relative to sarcomeres, size of T-system tubules, sarcomere length, muscle fiber diameter in native and fixed states, and size of myofibrils were measured in four straited muscle fiber types in Atlantic hagfishes (Myxine glutinosa, L.) of different sizes. Traids occur at A/I-injunctions in all fiber type...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00223227
更新日期：1975-01-01 00:00:00
abstract：:Electron-dense granules (EDGs) are singular structures found in the tissues of several vertebrate and invertebrate organisms. Two types of EDGs were observed in hepatopancreatic cells of the crab Ucides cordatus: (1) a non-mineralized EDG, found mainly inside vacuoles, which reacted positively to acid phosphatase and ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0646-4
更新日期：2003-01-01 00:00:00
abstract：:The ultrastructural changes induced by the topical application of retinol acetate on hamster cheek pouch epithelium were evaluated using stereological analysis. Electron micrographs were prepared of the basal and superficial regions of the nucleated cell layer of the epithelium obtained from 3 treated and 3 control an...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214783
更新日期：1982-01-01 00:00:00
abstract：:Myogenesis is a complex process in which committed myogenic cells differentiate and fuse into myotubes that mature into the muscle fibres of adult organisms. This process is initiated by a cascade of myogenic regulatory factors expressed upon entry of the cells into the myogenic differentiation programme. However, ext...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1364-1
更新日期：2012-06-01 00:00:00
abstract：:The presence of immunoreactive enkephalin, dynorphin, vasoactive intestinal polypeptide, cholecystokinin, substance P and neuropeptide Y in nerve fibers that project to the guinea-pig inferior mesenteric ganglion was analysed, after different denervation and ligation procedures. A quantitative analysis demonstrates th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219741
更新日期：1988-09-01 00:00:00
abstract：:Cobaltous-lysine is transported anterogradely from the optic nerve of the teleost, Lethrinus chrysostomus (Lethrinidae, Perciformes). The marginal optic tract is labelled in longitudinal bands of light and dark staining fibres which persists caudally within the ventral division but not in the dorsal division. This spe...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218889
更新日期：1989-01-01 00:00:00
abstract：:We used a combined biochemical and histocytochemical approach to study ecto-ATPase in the rat cardiac muscle. The reaction medium employed for histocytochemical detection was optimized in biochemical assays to achieve the highest enzyme activity and lowest inhibition by the capture agent used for visualization of the ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004419900105
更新日期：1999-12-01 00:00:00
abstract：:Affinity of lymphoid cells for the microenvironment of germinal centers (GC), as detectable in transfer experiments by rapid homing in spleen GC from the blood, is a capacity expressed by only a subset of lymphoid cells, in particular by those constituting a GC. However, when introduced into the blood stream, these ce...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215160
更新日期：1984-01-01 00:00:00
abstract：:The lymphatic organization and its relationship to the vascular system in the rat small intestine was studied by scanning electron microscopy of corrosion casts and freeze-fractured tissues, and by light microscopy of injected preparations. The villus possessed 3-10 or more central lacteals depending upon the villous ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218204
更新日期：1987-05-01 00:00:00
abstract：:The retina of the distal and proximal lens-bearing complex ocelli are composed of pigmented sensory cells and long pigmented cells. A ciliary sheath from each sensory cell, together with the processes of long pigmented cells, extends through the vitreous layer as far as the capsule that envelops the lens. Each ciliary...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219415
更新日期：1976-07-30 00:00:00
abstract：:Captopril [1-(D-3-mercapto-2-methyl-1-oxopropyl)-L-proline (S,S)] is now known to be an inhibitor of angiotensin-converting enzyme. In male mice, the juxtaglomerular cells were examined by electron microscopy 42 h after administration of captopril. Numerous dense granules appeared in the rough endoplasmic reticulum of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209980
更新日期：1981-01-01 00:00:00
abstract：:Two-month-old female Swiss mice that had come into estrus were injected intravenously with L-3H-fucose and killed at 5, 15, 40 min, and 4 h after injection. Pieces of the isthmus and of the ampulla of the uterine tubes were processed for light- and electron-microscopic radioautography. Incorporation of 3H-fucose was m...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220036
更新日期：1988-10-01 00:00:00
abstract：:Fibrosis is the result of a wound-healing response that fails to restore normal tissue structure function. One of the critical hallmarks of fibrosis is disrupted collagen remodeling. In tissue homeostasis, the production, deposition and organization of collagen is balanced by the degradation and remodeling of collagen...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-016-2440-8
更新日期：2016-09-01 00:00:00
abstract：:We develop "autologous bone marrow cell infusion (ABMi) therapy" for the treatment of human decompensated liver cirrhosis and confirm the efficacy and safety of this treatment in multicenter clinical studies. With the goal of further expanding the applications of ABMi, we first cultured human bone marrow cells and the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1727-2
更新日期：2013-12-01 00:00:00
abstract：:The occurrence of neurons possessing high-affinity uptake sites for GABA was studied in the myenteric plexus of the guinea-pig ileum, caecum, and proximal and distal colon, the rat proximal colon, and the chicken gizzard with the use of 3H-GABA and autoradiography. Experiments were carried out on plexuses that had bee...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217417
更新日期：1983-01-01 00:00:00
abstract：:The endoneurial collagen sheath around teased nerve fibres following crush injury was studied by scanning electron microscopy and compared with uninjured sciatic nerve fibres and with fibres from the dystonic mutant mouse. Following crush injury the endoneurial collagen became more abundant than seen in untreated nerv...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210469
更新日期：1981-01-01 00:00:00
abstract：:The elaboration and distribution of newly formed proteins in the striated muscle of 21-day-old mice were investigated by quantitative radioautography at intervals between 2 and 240 min after intravenous injection of tritiated leucine. In radioautographs, the localization and the relative label concentration were compa...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209040
更新日期：1978-10-17 00:00:00
abstract：:Activities of the H+, K(+)-ATPase and the Na+, K+, ATPase have been localized in the morphologically heterogeneous oxyntic cell lineage of adult bovine abomasal mucosa, by means of K(+)-dependent paranitrophenylphosphatase (K(+)-pNPPase) histochemistry. At the light- and electron-microscopic level, only members of the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050583
更新日期：1996-05-01 00:00:00
abstract：:Live Merkel cells in the skin and hair follicles are known to incorporate a fluorescence dye, quinacrine, which has been utilized to identify and dissect the cells for experiments. Quinacrine fluorescence of the cells is, however, quickly lost and quinacrine-stained Merkel cells soon become difficult to identify in ti...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0688-7
更新日期：2003-03-01 00:00:00
abstract：:The cardiovascular system is the first functional organ in the vertebrate embryo, and many organs start to develop adjacent to cells of the cardiovascular system. Endothelial cells (EC) form the inner cell lining of blood vessels and represent the major cell type that interacts with developing organs. On the one hand,...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-003-0739-8
更新日期：2003-10-01 00:00:00
abstract：:Loss of wild-type p53 activity is thought to be a major predictor of failure to respond to radiotherapy and chemotherapy in various human cancers. This assumption is largely based on some cell-death studies in p53-knockout mice and on correlations of p53 status assessed by immunochemistry or single-strand conformation...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410051072
更新日期：1998-06-01 00:00:00
abstract：:Myelin formation in cultures of previously dissociated spinal cord from foetal mice is described. In addition to the expected pattern of myelination, in which axons are closely wrapped by myelin lamellae, redundant folds of myelin have been found, as have double sheaths surrounding a single axon. Hypotheses concerning...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00223265
更新日期：1975-09-16 00:00:00
abstract：:It is possible to determine statistically the repartitions of shapes or sizes in a population of particles from the recurrence of shapes or sizes of their outlines in microscopical sections. This method was applied to a population of 1326 follicular profiles randomly sampled from 30 ovaries of pubescent homozygous "Nu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219416
更新日期：1976-07-30 00:00:00
abstract：:Recent studies demonstrated the expression of the insulin receptor (InsR) and its functional interaction with the transient receptor potential vanilloid type 1 receptor (TRPV1) in primary sensory neurons (PSNs). The present study was undertaken to reveal the target-specific expression of the InsR and its co-localizati...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2868-0
更新日期：2018-11-01 00:00:00
abstract：:The membrane systems of cardiac muscle cells of the euphausiacean Meganychtiphanes norvegica are described. Transverse tubules are found both at the Z-band level (TZ-tubules) and at the H-band level (Th-tubules). Within the sarcomere narrow longitudinal tubules branch off from the TZ-tubules. At the H-band level these...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00223109
更新日期：1977-07-15 00:00:00
