Electron-cytochemical localization of alkaline phosphatase to G cells of Necturus maculosus antrum.

Abstract:

:Electron-cytochemical localization of alkaline phosphatase activity was performed on G cells of Necturus maculosus antral mucosa. Alkaline phosphatase activity was localized to the nuclear membrane, the Golgi/endoplasmic reticulum, and the limiting membranes of G cell peptide-secretion vesicles. There was no specific localization of alkaline phosphatase activity to the plasma membrane. Treatment of the tissues with levamisole (an alkaline phosphatase inhibitor) did not markedly reduce the specific alkaline phosphatase activity. Specific lead deposition was reduced by removal of the substrate from the reaction mixture. The results from this study on N. maculosus G cells demonstrate that alkaline phosphatase activity can be found in a non-mammalian gastric endocrine cell and that specific activity was localized primarily to those intracellular structures involved with protein biosynthesis.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Rutten MJ,Garrison RG,Moore CD,Fiskin AM,Cheung LY

doi

10.1007/BF00218867

subject

Has Abstract

pub_date

1989-12-01 00:00:00

pages

555-61

issue

3

eissn

0302-766X

issn

1432-0878

journal_volume

258

pub_type

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