Antral G-cell in gastrin and gastrin-cholecystokinin knockout animals.

Abstract:

:The antral hormone gastrin is the key regulator of gastric acid secretion, mucosal growth and differentiation. Gastrin is synthesized in the endocrine G-cells in the antroduodenal mucosa. We have now examined the way in which the loss of gastrin alone or gastrin plus cholecystokinin (CCK) affects the antral G-cell. Immunohistochemistry, radioimmunoassay and quantitative real-time polymerase chain reaction techniques were employed to examine the expression of genes belonging to the G-cell secretory pathway in gastrin and gastrin-CCK knockout mice. Transmission electron microscopy was used to examine the ultrastructure of the G-cells. The number of G-cells increased but the secretory granules were few and abnormally small in the G-cells of both mouse models compared with wildtypes. Thus, gastrin is not necessary for the formation of G-cells as such but the lack of gastrin reduces the number and size of their secretory granules suggesting that gastrin is vital for the formation and/or maintenance of secretory granules in G-cells.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Friis-Hansen L,Wierup N,Rehfeld JF,Sundler F

doi

10.1007/s00441-005-1110-z

keywords:

subject

Has Abstract

pub_date

2005-07-01 00:00:00

pages

141-6

issue

1

eissn

0302-766X

issn

1432-0878

journal_volume

321

pub_type

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