Abstract：
:This study aimed to characterize porcine Achilles tendon (PAT) in terms of its structural components, vascularity, and resident tendon cells. We found that PAT is composed of a paratenon sheath, a core of fascicles, and an endotenon/interfascicular matrix (IFM) that encases the fascicle bundles. We analyzed each of these three tendon components structurally using tissue sections and by isolating cells from each component and analyzing in vitro. Many blood vessel-like tissues were present in the paratenon and IFM but not in fascicles, and the vessels in the paratenon and IFM appeared to be inter-connected. Cells isolated from the paratenon and IFM displayed characteristics of vascular stem/progenitor cells expressing the markers CD105, CD31, with α-smooth muscle actin (α-SMA) localized surrounding blood vessels. The isolated cells from paratenon and IFM also harbored abundant stem/progenitor cells as evidenced by their ability to form colonies and express stem cell markers including CD73 and CD146. Furthermore, we demonstrate that both paratenon and IFM-isolated cells were capable of undergoing multi-differentiation. In addition, both paratenon and IFM cells expressed elastin, osteocalcin, tubulin polymerization promoting protein (TPPP), and collagen IV, whereas fascicle cells expressed none of these markers, except collagen I. The neurotransmitter substance P (SP) was also found in the paratenon and IFM-localized surrounding blood vessels. The findings of this study will help us to better understand the vascular and cellular mechanisms of tendon homeostasis, injury, healing, and regeneration.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Zhang J,Li F,Williamson KM,Tan S,Scott D,Onishi K,Hogan MV,Wang JHdoi
10.1007/s00441-020-03379-3subject
Has Abstractpub_date
2021-01-26 00:00:00eissn
0302-766Xissn
1432-0878pii
10.1007/s00441-020-03379-3pub_type
杂志文章abstract：:By using an antiserum raised against a crustacean beta-pigment-dispersing hormone (PDH), the distribution and chemical neuroanatomy of PDH-like immunoreactive neurons was investigated in the central nervous system of the gastropod snails, Helix pomatia and Lymnaea stagnalis. The number of immunoreactive cells in the H...
journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216347
更新日期：1985-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1976-07-26 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2017-02-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-1144-2
更新日期：2005-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1137-2
更新日期：2011-05-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00223217
更新日期：1978-07-13 00:00:00
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journal_title：Cell and tissue research
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doi：10.1007/s00441-018-2868-0
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
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pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
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更新日期：2015-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1992-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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