Ultrastructural identification of non-adrenergic, non-cholinergic nerves in the rat anococcygeus muscle.

Abstract:

:The innervation of the rat anococcygeus muscle has been investigated ultrastructurally following fixation with a modified chromaffin reaction for the demonstration of biogenic amines (Tranzer and Richards, 1976). Three types of nerve profiles were revealed: (1) 60-70% of the profiles are adrenergic; (2) less than 5% of the profiles appear to be cholinergic; (3) up to 40% of the profiles are distinguished by the presence of a characteristically high proportion of electron-opaque, chromaffin-negative vesicles, 85-110 nm in diameter. This third type of profile was not affected by 6-OHDA, and is considered to represent the non-adrenergic, non-cholinergic inhibitory innervation of this tissue. Because of the morphological similarity of this nerve type, apart from the smaller vesicle size, to classical peptidergic nerve endings, they have been termed "small p-type" (sp-type). These results are discussed in relation to a previous report describing only two types of nerve profiles in this tissue (Gillespie and Lüllmann-Rauch, 1974).

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Gibbins IL,Haller CJ

doi

10.1007/BF00236418

subject

Has Abstract

pub_date

1979-08-01 00:00:00

pages

257-71

issue

2

eissn

0302-766X

issn

1432-0878

journal_volume

200

pub_type

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