Immune complexes of E. coli antigens and maternal IgG in the bursa of Fabricius.

Abstract:

:The bursa of Fabricius of the chicken is known to be both a primary lymphoid organ and a secondary lymphoid tissue. Bursal follicles are equipped with antigen-trapping follicle-associated epithelium. However, bioactive antigens such as protein and bacteria have not been detected in the bursal parenchyma. By immunoperoxidase staining with a polyspecific antibody (Ab) against Escherichia coli, we detected aggregated E. coli antigens in the medulla of bursal follicles after hatching. The distribution of aggregated E. coli antigens is restricted to the medulla of bursal follicles. The antigens are not found in the spleen or the parenchyma of the caecal tonsil. The bursa is thus a trapping site for E. coli antigens from the external environment. Furthermore, two-color immunostaining clarified that these antigens form immune complexes with maternal IgG (MIgG) and are retained by reticular cells. Additionally, immune complexes in the bursa were shown to induce the rapid development of serum IgM Ab for indigenous E. coli. Our results suggest that immune complexes of MIgG and environmental antigens in the medulla of bursal follicles exert positive effects on B-cell differentiation in the bursa in situ.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Sonoda K,Noguchi K,Ekino S

doi

10.1007/s00441-013-1701-z

subject

Has Abstract

pub_date

2013-12-01 00:00:00

pages

813-21

issue

3

eissn

0302-766X

issn

1432-0878

journal_volume

354

pub_type

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