Abstract：
:Dysfunction of the retinal pigmented epithelium (RPE) is one of the first effects of dry age-related macular degeneration (AMD) with consequent blindness. Hence, patients affected by this retinal disorder could benefit from a cell-based transplantation strategy for RPE. Actually, an effective protocol to approach this problem is lacking, though recently, it has been postulated the existence of a subpopulation of RPE stem cells (RPESCs) derived from adult RPE and able to reconstitute a functional RPE. On the other hand, the evidence related to the differentiative potential of human mesenchymal stem cells (MSCs) is continuously increasing. Among others, amniotic fluid-derived MSCs (AF-MSCs) may be a promising candidate, since these cells are characterized by high proliferation and differentiative potential. In this study, AF-MSCs and RPESCs were isolated, characterized to assay their stemness and induced to neuronal/retinal differentiation; specific RPE markers were then analyzed. Our results indicate that RPESCs are more suitable candidates for RPE replacement than AF-MSCs.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Mariotti C,Lazzarini R,Nicolai M,Saitta A,Orsini E,Orciani M,Di Primio Rdoi
10.1007/s00441-015-2185-9subject
Has Abstractpub_date
2015-10-01 00:00:00pages
21-31issue
1eissn
0302-766Xissn
1432-0878pii
10.1007/s00441-015-2185-9journal_volume
362pub_type
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