Abstract:
:The molecular cloning of a cDNA fragment synthesised from rat duodenal mRNA coding for cholecalcin (calbindin), a 9000 Mr vitamin D-induced calcium-binding protein (CaBP), has been previously described. DNA/RNA hybridisation assays have been used to examine CaBP mRNA production in the uterine horns and duodena of pregnant (21 day) rats using the cloned CaBP cDNA. Northern hybridisation studies showed that the 32P cDNA sequence hybridised to a single 500-600 nucleotide species in both the uterus and the duodenum, thus demonstrating identical CaBP mRNA processing in both tissues. Dot blot hybridisation studies showed that the CaBP mRNA concentration was greatest in the duodenum while that of the uterine horns was about 10% of the duodenal level. The observed differences in CaBP mRNA levels correlate well with the in vivo CaBP concentrations. In situ hybridisation histochemistry using 3H cDNA revealed that CaBP mRNA visualised by silver grains was found in all the parts of the endometrium and the myometrium. However, CaBP mRNA was more concentrated in the outer and inner muscular fibres and in the luminal cells of the endometrium than in the stroma cells. These results demonstrate that the CaBP gene is expressed in specific cells of the rat uterus.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Warembourg M,Perret C,Thomasset Mdoi
10.1007/BF00216546subject
Has Abstractpub_date
1987-01-01 00:00:00pages
51-7issue
1eissn
0302-766Xissn
1432-0878journal_volume
247pub_type
杂志文章abstract::Striated rootlets in ciliated cells are conical banded structures composed of longitudinally aligned filaments. The formation of striated rootlets during ciliognesis in the human oviduct epithelium was studied by electron microscopy. Primitive rootlets appeared at the proximal side of basal bodies before or at the sam...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050905
更新日期:1997-10-01 00:00:00
abstract::The octavo-lateral efferent system of several anuran species was studied by means of retrograde transport of horseradish peroxidase. This system is organized similarly in all larval anurans and in all adult aglossids. All have two groups of efferent neurons in the nucleus reticularis medialis between the VIIIth and th...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214812
更新日期:1982-01-01 00:00:00
abstract::The endothelial layer of blood vessels controls the passage of cells and solutes from the blood into the surrounding tissue. Crucial for this regulation is the integrity of endothelial cell-cell junctions. Various molecular mechanisms control junctional integrity of the endothelial layer including GTPases, modulation ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-014-1812-1
更新日期:2014-03-01 00:00:00
abstract::We have examined radioautographically the protein synthetic and secretory activity of differentiating odontoblasts and ameloblasts, exposed for 9 h in vitro to various concentrations of colchicine in the presence of 3H-proline. Colchicine impairs the cytodifferentiation of the dental epithelium into ameloblasts and of...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216651
更新日期:1988-06-01 00:00:00
abstract::In rats the "fast" fibular nerve was transposed to the "slow" soleus muscle outside the original innervation band. Formation of new neuromuscular junctions was induced by cutting the soleus nerve after different periods of time. The morphological maturation of these junctions was studied by electron microscopy. New ne...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215176
更新日期:1976-04-09 00:00:00
abstract::Ependymal cells and their junctional complexes in the area postrema of the rat were studied in detail by tracer experiments using horseradish peroxidase (HRP) and colloidal lanthanum and by freeze-etch techniques, in addition to routine electron microscopy. The ependyma of the area postrema is characterized as flatten...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00235058
更新日期:1979-09-03 00:00:00
abstract::Mechanisms underlying the unique survival property of human spiral neurons are yet to be explored. P75 (p75(NTR)) is a low affinity receptor for neurotrophins and is known to interact with Trk receptors to modulate ligand binding and signaling. Up-regulation of this receptor was found to be associated with apoptosis a...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-012-1395-7
更新日期:2012-06-01 00:00:00
abstract::Male/female differences in enzyme activity and gene expression in the liver are known to be attenuated with ageing. Nevertheless, the effect of ageing on liver structure and quantitative cell morphology remains unknown. Male and female Wistar rats aged 2, 6, 12 and 18 months were examined by means of stereological tec...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-016-2490-y
更新日期:2016-12-01 00:00:00
abstract::Noses are extremely sophisticated chemical detectors allowing animals to use scents to interpret and navigate their environments. Odor detection starts with the activation of odorant receptors (ORs), expressed in mature olfactory sensory neurons (OSNs) populating the olfactory mucosa. Different odorants, or different ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-020-03327-1
更新日期:2021-01-06 00:00:00
abstract::Changes in the frequency of endocrine cells are evidence of intestinal adaptation to germ-free (GF) status. Not only the distribution of these cells along the intestine, but also the differences in intracellular content of these regulatory peptides may be explored to explain functional and structural aspects of GF int...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410000323
更新日期:2001-03-01 00:00:00
abstract::Rat blood monocytes contain granules of two types, primary and secondary granules, which can be distinguished on morphological grounds. Secondary granules have a halo between the limiting membrane and the matrix; primary granules have no halo and are generally more elongate than the secondary granules. In addition, th...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00226665
更新日期:1977-11-30 00:00:00
abstract::A monoclonal antibody (Mab) developed against a partially purified bursal protein extract was found to bind specifically to a single cell type in the cortico-medullary border region of the chicken bursa of Fabricius. These cells were microscopically similar to the bursal secretory dendritic-like cells. A product with ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-0910-x
更新日期:2004-09-01 00:00:00
abstract::The acrosome is a special organelle that develops from the Golgi apparatus and the endolysosomal compartment in the spermatids. Centromere protein E (CENP-E) is an essential kinesin motor in chromosome congression and alignment. This study is aimed at investigating the roles and mechanisms of kinesin-7 CENP-E in the f...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-020-03341-3
更新日期:2020-11-25 00:00:00
abstract::Transcriptome profiling of the pineal gland has revealed night/day differences in the expression of a major fraction of the genes active in this tissue, with two-thirds of these being nocturnal increases. A set of over 600 transcripts exhibit two-fold to >100-fold daily differences in abundance. These changes appear t...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-010-1094-1
更新日期:2011-04-01 00:00:00
abstract::The distribution of radioactivity in the rat hypothalamus after intraventricular injection of tritiated luteinizing hormone-releasing hormone (LH-RH) was studied by autoradiography and compared with that expected from Fick's second law which defines the character of ordinary diffusion. The results suggest that LH-RH p...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215317
更新日期:1976-05-13 00:00:00
abstract::Horseradish peroxidase (HRP) was injected into the left dorsal column nuclei of adult cats. Large dorsal funiculi axons of the C3, C5, C8 and L7 segments were searched for HRP-activity after 12, 24, 36 and 48h using light and electron microscopy. Accumulations of intra-axonal HRP-positive bodies occurred at nodes of R...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050513
更新日期:1996-01-01 00:00:00
abstract::Thyroid glands of 7 to 21 day-old chick embryos were examined by electron microscopy using freeze-fracture and thin-section preparations. The primitive follicle lumen first appears between two adjacent epithelial cells in 8 day-old embryos, and is formed in the region of a focal tight junction (macula occludens). The ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00234830
更新日期:1979-04-30 00:00:00
abstract::Using histochemical, immunohistochemical and biochemical techniques, noradrenaline-, neuropeptide Y-, vasoactive intestinal polypeptide-, substance P- and calcitonin gene-related peptide-containing nerve fibres were studied in the uterine artery of virgin, progesterone-treated and pregnant guinea-pigs. Morphological c...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF01740777
更新日期:1990-03-01 00:00:00
abstract::The vacuoles occurring in rat hepatocytes after intraportal injection of retinol (33 or 67 micrograms) were examined immunohistochemically using respective antibodies against rat albumin, human retinol-binding protein, human ceruloplasmin, human alpha 1-antitrypsin, human transferrin, and human prealbumin as represent...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00261829
更新日期:1989-08-01 00:00:00
abstract::The occurrence of neurons possessing high-affinity uptake sites for GABA was studied in the myenteric plexus of the guinea-pig ileum, caecum, and proximal and distal colon, the rat proximal colon, and the chicken gizzard with the use of 3H-GABA and autoradiography. Experiments were carried out on plexuses that had bee...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217417
更新日期:1983-01-01 00:00:00
abstract::The problem of the blood-brain barrier in the pineal organ of the rainbow trout, Salmo gairdneri, was investigated following intraperitoneal or intracardial injections of several tracers and dyes with different molecular weights. As demonstrated at the light-microscopic level, repeated injections of trypan blue or hor...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219238
更新日期:1985-01-01 00:00:00
abstract::Fibrosis is the result of a wound-healing response that fails to restore normal tissue structure function. One of the critical hallmarks of fibrosis is disrupted collagen remodeling. In tissue homeostasis, the production, deposition and organization of collagen is balanced by the degradation and remodeling of collagen...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-016-2440-8
更新日期:2016-09-01 00:00:00
abstract::Synaptic ribbons in photoreceptor cells of the goldfish pineal organ undergo significant daily changes in their length, distance from the plasma membrane, and number per unit area of pineal end-vesicle. The rhythms persist in fish exposed to constant darkness. Constant light abolishes the rhythms in length and distanc...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233525
更新日期:1981-01-01 00:00:00
abstract::Whether an axon will regenerate after it is cut depends on the balance between the intrinisic ability of the axon to regrow and the permissiveness of the environment surrounding it. The permissiveness of the environment is determined by the glial cells at the site of injury, and in the CNS both oligodendrocytes and as...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s004410050943
更新日期:1997-11-01 00:00:00
abstract::This work describes the origin and development of about 200 tension receptor cells located around the anterior attachment site of the locust ovipositor muscle and their migration to their final position on the muscle fibres. The locust ovipositor muscle is the only insect system in which more than 100 tension receptor...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-007-0498-z
更新日期:2007-12-01 00:00:00
abstract::The fine structure of the horny teeth of the lamprey, Entosphenus japonicus, was examined by light- and electron-microscopy. Most of the horny teeth consisted of two horny and two nonhorny layers. The primary horny layer was well keratinized, and the cells were closely packed and intensely interdigitated, being joined...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215769
更新日期:1983-01-01 00:00:00
abstract::A culture technique for the in vitro growth and differentiation of antennal cells of Mamestra brassicae is described. The morphology of the growing cells is described. At least five morphological cell types can be distinguished in the cultures. Neuronal cells (type A) were characterized morphologically and immunocytoc...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050884
更新日期:1997-08-01 00:00:00
abstract::Endothelial repair to reestablish structural integrity following wounding is a complex process. Since the actin cytoskeleton undergoes specific changes in distribution as quiescent endothelial cells switch to activated migrating cells over a 6-h period following wounding (Lee et al. 1996), we studied tyrosine phosphor...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051370
更新日期:1999-09-01 00:00:00
abstract::Previous studies have identified Dogiel type II neurons with cell bodies in the myenteric plexus of guinea-pig ileum to be intrinsic primary afferent neurons. These neurons also have distinctive electrophysiological characteristics (they are AH neurons) and 82-84% are immunoreactive for calbindin. They are the only ca...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410100395
更新日期:2001-07-01 00:00:00
abstract::Rainbow trout (Oncorhynchus mykiss) were exposed to ion-poor (soft) water to test the hypothesis that naturally induced proliferation of branchial chloride cells causes a thickening of the blood-to-water diffusion barrier. This was achieved by using a combination of scanning and transmission electron-microscopic techn...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050622
更新日期:1996-07-01 00:00:00