Abstract:
:Scanning electron microscopy was used to investigate the morphological changes of the tail musculature of the metamorphosing anuran tadpole, attention being focused on phagocytosis by macrophages. Muscle fibers were stained en bloc with silver and freeze-fractured during dehydration, or torn after drying. Samples were sputter-coated with gold-palladium and observed in both secondary electron- and back-scattered electron modes with a scanning electron microscope. Various cells were identified by the methods of secondary electron- and back-scattered electron images. Some macrophages lying between muscle fibers at prometamorphic stages possessed numerous finger-like projections and well-developed ruffles. During degeneration of muscle fibers macrophages collected in the degenerating region and invaded the space between the disordering myofibrils. In advanced stages the numbers of macrophages clearly increased on or around the degenerating muscle fibers. At the climactic stage fragmented muscles were entrapped and then engulfed by the macrophages. With the completion of phagocytosis, the macrophages became globular with reduction of the ridge-like ruffles. Macrophages may play a role not only in scavenging the fragmented muscle fibers, but also using their long processes in active formation of the fragments.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Watanabe K,Horiguchi T,Sasaki Fdoi
10.1007/BF00214574subject
Has Abstractpub_date
1985-01-01 00:00:00pages
545-50issue
3eissn
0302-766Xissn
1432-0878journal_volume
241pub_type
杂志文章abstract::The medial preoptic nucleus is a sexually dimorphic structure whose cytoarchitecture, afferent and efferent connections, and functions have been previously described. No detailed ultrastructural study has, however, been performed to date. Here we describe the ultrastructural organization of this important preoptic str...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00318164
更新日期:1995-03-01 00:00:00
abstract::The antigen detected by the rat anti-mouse monoclonal antibody (m Ab), anti-BSP-3, has been initially described as a brain cell-surface protein. Evidence is presented that this m Ab recognizes mouse (Na+ + K+)-ATPase (ATP phosphohydrolase, E.C.3.6.1.3). The antigen, purified from mouse brain by means of affinity chrom...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218655
更新日期:1983-01-01 00:00:00
abstract::With regard to the protein content, as analysed cytophotometrically, of hepatocytes from rats kept under a 12L 12D photoperiod (photophase 7:00-19:00), the following facts have been established: 1) Hepatocytes of different classes of ploidy all demonstrate, more or less equally, daily variations in protein content and...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221865
更新日期:1986-01-01 00:00:00
abstract::In developing bioassays for estrogenic effects, vitellogenin (Vtg) induction and zona radiata protein (Zr-protein) induction in males and juveniles of oviparous vertebrates have been used as sensitive biomarkers for estrogenicity. Nonylphenol (NP) produces similar and parallel expression patterns of Vtg and Zr-protein...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-007-0543-y
更新日期:2008-03-01 00:00:00
abstract::The effects of dexamethasone on the ability of cells enzymatically isolated from 21-day fetal rat calvaria to produce cartilage in vitro has been investigated. Primary cultures of single-cell suspensions of rat calvaria were grown for up to 28 days in vitro in alpha-minimal essential medium containing 15% fetal bovine...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224728
更新日期:1989-04-01 00:00:00
abstract::Testicular interstitial cells, from rats aged 35 days, were dispersed with collagenase and separated through Percoll into 5 fractions (I-V); fraction I being the least dense. Measurement of basal testosterone production, histo-enzymological staining for 3 beta-hydroxysteroid dehydrogenase activity and electron microsc...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222304
更新日期:1988-08-01 00:00:00
abstract::Pax genes are expressed in specific patterns in the nervous system during development and in the adult. Recent findings suggest a link between the expression of Pax-2 and axonal guidance. Mice with a targeted deletion of Pax-2 are an excellent tool for studying axonal pathfinding at the molecular level, especially wit...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s004410050923
更新日期:1997-11-01 00:00:00
abstract::The presence, location and degree of immunoexpression of various microfilament (MF) and intermediate filament (IF) systems (actin, cytokeratins, desmin, vimentin) were studied in the excurrent ducts of the testis in sexually mature and active galliform (Japanese quail, domestic fowl, turkey) and anseriform (duck) bird...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-008-0631-7
更新日期:2008-08-01 00:00:00
abstract::To investigate the mechanisms whereby bone cells absorb organic bone-matrix components during endochondral bone development, rat humeri were examined, employing horseradish peroxidase as a soluble protein tracer. Intravenously-injected peroxidase filled the osteoid layer and penetrated into the osteocyte lacunae and c...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219233
更新日期:1985-01-01 00:00:00
abstract::Although the pharmacological properties and distribution of inositol 1,4,5-trisphosphate (IP3)-sensitive calcium stores vary considerably among tissues, studies of its localization have been devoted mostly to the central nervous system. In this report we have analyzed the localization of IP3 receptors in diverse non-n...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050641
更新日期:1996-08-01 00:00:00
abstract::The papillary muscle of the cat heart's right ventricle has not been studied previously with quantitative ultrastructural techniques despite its wide use for functional studies. This tissue was perfusion-fixed, processed for electron microscopy, and morphometric techniques were used to assess the ultrastructural chara...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216200
更新日期:1983-01-01 00:00:00
abstract::The amniotic epithelium constitutes a diffusion barrier controlling the passage of solutes and water between the amniotic cavity and maternal circulation. With the present immunocytochemical approach, we have shown that several major components of the cyto-skeleton, i.e., actin, alpha-actinin, spectrin and ezrin, are ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00318194
更新日期:1991-11-01 00:00:00
abstract::The thyroid cells of the cream hamster, characterized by abundance of microtubules and stratification of the organelles, undergo a particular evolution when the animals grow older. These changes are characterized by an increase of the number of lysosomes which in extreme cases become so prominent that they occupy the ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215124
更新日期:1976-02-06 00:00:00
abstract::Lysozyme is one of the most prominent antimicrobial peptides and has been identified from many mammalian species. However, this enzyme has not been studied in the order Insectivora, which includes the most primitive placental mammals. Here, we done the lysozyme cDNA from Suncus murinus (referred to as suncus, its labo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-019-02991-2
更新日期:2019-06-01 00:00:00
abstract::Electron-microscopic evidence indicates that during conditions of high hormone demand such as lactation there is a dramatic reduction in the number of fine glial processes which are normally interposed between magnocellular neuroendocrine cell somata in the supraoptic nucleus (SON). The purpose of this study was to co...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00225557
更新日期:1985-01-01 00:00:00
abstract::1,25-Dihydroxyvitamin D3 and 24,25-dihydroxyvitamin D3 have antagonistic effects on the acellular bone of the tilapia Sarotherodon mossambicus. 1,25-Dihydroxyvitamin D3 inhibits the activity of the lining osteoblasts. Prolonged administration leads to demineralization of the bone matrix. Injection of 24,25-dihydroxyvi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00206270
更新日期:1983-01-01 00:00:00
abstract::A histological examination of 205 fish representing four cyprinid species from a site 2.5 miles north of Wheeling, West Virginia, on the Ohio River revealed large (2--4 micron) cuboidal intranuclear inclusion bodies (NIB's) within neurons in the cranial and spinal ganglia of three species. Because the minnows had been...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00210112
更新日期:1981-01-01 00:00:00
abstract::In plants, intercellular structures named plasmodesmata (PD) form a continuous cytoplasmic network between neighboring cells. PD pores provide channels for intercellular symplasmic (cell-to-cell) transport throughout most tissues of the plant body. Cell-defining proteins, such as transcription factors, and regulatory ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-012-1550-1
更新日期:2013-04-01 00:00:00
abstract::Antithrombin (AT), which is mainly synthesized in the liver, is an acute-phase plasma protein in mammalian species. Here, we demonstrated that sheep anti-human AT antibody cross-reacted with the humoral fluids in amphioxus Branchiostoma belcheri tsingtauense as well as human serum. The concentration of AT in the humor...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-0088-x
更新日期:2006-03-01 00:00:00
abstract::Growth hormone, prolactin and somatostatin-like immunoreactivities were demonstrated in the brains of larval, young adult (parasitic) and upstream migrant adult sea lampreys, Petromyzon marinus, by means of immunoperoxidase techniques. Growth hormone (GH) and prolactin (PRL) were observed within separate perikarya in ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218994
更新日期:1986-01-01 00:00:00
abstract::Adenosine 5'-triphosphate is known to function as a potent extracellular messenger, producing its effects via a distinct family of cell surface receptors. Different receptor subtypes have been shown to modulate different cellular functions such as proliferation, differentiation and apoptosis. We have investigated the ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-1149-x
更新日期:2005-09-01 00:00:00
abstract::The ultrastructural immunohistochemical localization of gamma aminobutyric acid (GABA) and its regulating enzymes, L-glutamate decarboxylase (GAD) and gamma aminobutyrate-alpha-ketoglutarate transaminase, was determined utilizing an immunogold post-embedding protocol in pancreatic exocrine tissue. Within the acinar ce...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00213841
更新日期:1988-04-01 00:00:00
abstract::Hepatic peroxisomes in human embryos with a menstrual age of 6 and 7 weeks have been examined via catalase cytochemistry. In the younger sample, the organelles show no catalase activity, their matrix being pale and coarsely reticular. In the 7-week specimen, the peroxisome population consists of catalase-positive and ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00323574
更新日期:1993-04-01 00:00:00
abstract::Endothelins (ET) are a family of vasoactive peptides that act via two subtypes of receptors, named ETA and ETB. ET-1 binds to both ETA and ETB, whereas the isopeptide ET-3 preferentially binds to ETB. The localization of ETA and ETB receptors in the rat adrenal gland and their involvement in the adrenal secretagogue e...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050820
更新日期:1997-05-01 00:00:00
abstract::With an indirect immunofluorescence technique and an antiserum raised against synthetic LHRH, immunofluorescent granules were observed in fine radially oriented fibres in the palisade layer of the zoma externa in both the anterior and the posterior divisions of the median eminence in the greenfinch. There was no speci...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00223264
更新日期:1975-09-16 00:00:00
abstract::A technique has been developed to obtain viable, isolated and enriched populations of gastrin cells (G-cells) from the rat stomach. Restricted tissue samples from a small area of the pyloric antrum known to be particularly rich in G-cells, were sequentially digested with pronase followed by mechanical agitation, to re...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00236409
更新日期:1979-08-01 00:00:00
abstract::In an organ culture system under a three-dimensional microenvironment that provides the conditions needed for odontoblast differentiation, a row of odontoblasts can be induced (Kikuchi et al. 1996, 2001). Therefore, in a newly designed three-dimensional cell culture system that fulfils the conditions necessary for odo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-0882-x
更新日期:2004-08-01 00:00:00
abstract::Large granular lymphocytes (LGL) comprise a natural defense system in the liver and exert an inhibitory effect on tumor cell metastasis. In order to demonstrate the maturation of LGL in the liver from the morphological aspect, we evaluated electron-microscopically the frequency of 0.2 micron vesicles (rod-cored and "e...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00354799
更新日期:1994-04-01 00:00:00
abstract::Using anti-carp-gonadotropic-gamma-globulin, the indirect immunofluorescence technique was applied to sections of the pituitary of 12 teleost species. From the investigated species, the Poeciliinae, and especially the black molly, showed a distinct localization of fluorescent cells in the mesoadenohypophysis. Strong f...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224328
更新日期:1976-03-16 00:00:00
abstract::Ependymal cells and their junctional complexes in the area postrema of the rat were studied in detail by tracer experiments using horseradish peroxidase (HRP) and colloidal lanthanum and by freeze-etch techniques, in addition to routine electron microscopy. The ependyma of the area postrema is characterized as flatten...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00235058
更新日期:1979-09-03 00:00:00