Abstract：
:Adenosine 5'-triphosphate is known to function as a potent extracellular messenger, producing its effects via a distinct family of cell surface receptors. Different receptor subtypes have been shown to modulate different cellular functions such as proliferation, differentiation and apoptosis. We have investigated the functional expression and apoptotic action of the P2 X (7) receptor in human malignant melanoma tissue and cells. Incubation of cells with the potent P2 X (7) receptor agonist 2'-3'-O-(4-benzoyl-benzoyl) adenosine 5'-triphosphate leads to a decrease in cell number, which is dose-dependent and reversible by the antagonist 1-N,O-bis-[5-isoquinoline-sulfonyl]-N-methyl-L-tyrosyl)-4-phenyl-piperazine. Synthesis of the P2 X(7) receptor by these cells has been established by reverse transcriptase-polymerase chain reaction, immunohistochemistry, immunocytochemistry and cellular accumulation of the fluorescent DNA-binding dye YO-PRO-1. The P2 X(7) receptors have been shown to mediate apoptotic actions of extracellular nucleotides and represent a novel target for melanoma therapy.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
White N,Butler PE,Burnstock Gdoi
10.1007/s00441-005-1149-xkeywords:
subject
Has Abstractpub_date
2005-09-01 00:00:00pages
411-8issue
3eissn
0302-766Xissn
1432-0878journal_volume
321pub_type
杂志文章abstract：:Rat quiescin/sulphydryl oxidase (rQSOX) introduces disulphide bridges into peptides and proteins with the reduction of molecular oxygen to hydrogen peroxide. Its occurrence has been previously highlighted in a wide range of organs by reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot analyses, ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0043-x
更新日期：2006-01-01 00:00:00
abstract：:In the rostral hypothalamus of the domestic fowl, the magnocellular neurosecretory nuclei show a peculiar differentiation. Golgi studies of the supraoptic and paraventricular nuclei of the fowl reveal at least two major cell types: 1) large multipolar neurons, and 2) small interneurons. Golgi impregnations provide a d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236253
更新日期：1981-01-01 00:00:00
abstract：:The postnatal maturation of regions of the epididymis and intragonadal segment of the deferens duct was studied in the rat by light- and transmission electron microscopy. Maturation of the genital duct starts in the distal cauda epididymidis and ductus deferens after one week of life, and one week later, in the more c...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215508
更新日期：1987-08-01 00:00:00
abstract：:Osmotic stimulation activates both estivated and inactivated specimens of Helix pomatia and increases their central arousal. High-pressure liquid chromatography has shown that, during activation, the level of both serotonin and dopamine decreases in the central nervous system (CNS) but increases in the foot and heart,...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0522-3
更新日期：2008-02-01 00:00:00
abstract：:Sox2-expressing stem/progenitor cells in the anterior lobe of the pituitary gland form two types of micro-environments (niches): the marginal cell layer and dense cell clusters in the parenchyma. In relation to the mechanism of regulation of niches, juxtacrine signaling via ephrin and its receptor Eph is known to play...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-017-2646-4
更新日期：2017-10-01 00:00:00
abstract：:Call-Exner bodies are present in ovarian follicles of a range of species including human and rabbit, and in a range of human ovarian tumors. We have also found structures resembling Call-Exner bodies in bovine preantral and small antral follicles. Hematoxylin and eosin staining of single sections of bovine ovaries has...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051298
更新日期：1999-05-01 00:00:00
abstract：:Human endothelial progenitor cells (EPCs) were isolated from cord blood samples and enriched by magnetic activated cell sorting method based on the CD133 marker. Cells were incubated with different doses of bacterial lipopolysaccharide, ranging from 2, 5, 10, 50, 100, 200, 250, 500, to 1000 µg/ml, for 48 h. The cell s...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03405-4
更新日期：2021-01-18 00:00:00
abstract：:To investigate the mechanisms whereby bone cells absorb organic bone-matrix components during endochondral bone development, rat humeri were examined, employing horseradish peroxidase as a soluble protein tracer. Intravenously-injected peroxidase filled the osteoid layer and penetrated into the osteocyte lacunae and c...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219233
更新日期：1985-01-01 00:00:00
abstract：:Changes in the ultrastructure of the toad epidermis during the moulting cycle are described on the basis of 17 skin preparations fixed in consecutive phases of the cycle. Our previous light microscopical findings that morphological changes are mainly restricted to a short period prior to and after shedding are confirm...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221703
更新日期：1975-06-24 00:00:00
abstract：:Progenitor stem cells have been identified, isolated and characterized in numerous tissues and organs. However, their therapeutic potential and the use of these stem cells remain elusive except for a few progenitor cells from bone marrow, umbilical cord blood, eyes and dental pulp. The use of bone marrow-derived hemat...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-012-1471-z
更新日期：2012-10-01 00:00:00
abstract：:The mole has a single pair of accessory sex organs with features of both the prostate and the seminal vesicle, for which the term prostate gland is not appropriate. Seasonal changes occuring in this gland were related to four periods: a) the quiescence period, b) the maturation period, c) the active period and d) the ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218718
更新日期：1976-12-10 00:00:00
abstract：:Endothelial repair to reestablish structural integrity following wounding is a complex process. Since the actin cytoskeleton undergoes specific changes in distribution as quiescent endothelial cells switch to activated migrating cells over a 6-h period following wounding (Lee et al. 1996), we studied tyrosine phosphor...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051370
更新日期：1999-09-01 00:00:00
abstract：:Trichoplax, a member of the phylum Placozoa, is a tiny ciliated marine animal that glides on surfaces feeding on algae and cyanobacteria. It stands out from other animals in that it lacks an internal digestive system and, instead, digests food trapped under its lower surface. Here we review recent work on the phenotyp...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-019-03057-z
更新日期：2019-09-01 00:00:00
abstract：:Neurosecretory pathways were examined in normal male rats by the use of the immunoperoxidase technique for the localization of neurophysin in Bouin-fixed, deparaffinized sections. Using this technique two projections of extrahypothalamic neuorosecretory fibers can be traced to the sites of origin of the choid plexuses...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00232829
更新日期：1977-03-01 00:00:00
abstract：:The distributions within the coeliac ganglion of different chemically coded subgroups of noradrenaline neurons, and the relationships between these neurons and nerve fibres projecting to the ganglion from the intestine, have been assessed quantitatively by use of an immunohistochemical double-staining method. Noradren...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218395
更新日期：1986-01-01 00:00:00
abstract：:An antiserum against the octapeptide Pea-CAH-I, a member of the adipokinetic hormone/red pigment-concentrating hormone family, has been produced for immunocytochemical staining in insects and various other invertebrate species. The anti-Pea-CAH-I serum stains the glandular corpora cardiaca cells of those insect specie...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050601
更新日期：1996-06-01 00:00:00
abstract：:In the pond snail Lymnaea stagnalis, the growth regulating system consists of (1) about 200 neuroendocrine light green cells, located in four clusters in the cerebral ganglia, and (2) the paired canopy cells, located in the lateral lobes. These cells express genes encoding the molluscan insulin-related peptides (MIPs)...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00384739
更新日期：1992-07-01 00:00:00
abstract：:Osteoblasts are specialized mesenchyme-derived cells accountable for bone synthesis, remodelling and healing. Differentiation of osteoblasts from mesenchymal stem cells (MSC) towards osteocytes is a multi-step process strictly controlled by various genes, transcription factors and signalling proteins. The aim of this ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-013-1750-3
更新日期：2014-01-01 00:00:00
abstract：:Long-non-coding RNA (lncRNA) regulates gene expression through transcriptional and epigenetic regulation as well as alternative splicing in the nucleus. In addition, regulation is achieved at the levels of mRNA translation, storage and degradation in the cytoplasm. During recent years, several studies have described t...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-014-1885-x
更新日期：2014-06-01 00:00:00
abstract：:We conducted an immunoelectron-microscopic study for histamine (HA) in the enterochromaffin-like (ECL) cells of normal rats and rats given alpha-fluoromethylhistidine (alpha-FMH, 3 mg/kg per hour) via osmotic minipumps over a period of 24 h. The indirect immunoperoxidase procedure utilized a mouse monoclonal antibody ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100448
更新日期：2001-11-01 00:00:00
abstract：:MN9, a monoclonal antibody raised against mouse spermatozoa, specifically recognizes the equatorial segment of sperm head in several mammalian species, including humans. Colloidal gold-immuno-electron microscopy of mouse spermatozoa has shown that the antigen is localized in the space between the outer and inner acros...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00645047
更新日期：1992-12-01 00:00:00
abstract：:Transcription factor p53 regulates the cell cycle and apoptosis and may impair angiogenesis by the deregulation of pro-angiogenic factors and the activation of anti-angiogenic factors. Our aim has been to elucidate further the role of p53 in physiological angiogenesis. By treating hamsters with the wildtype p53 inhibi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-1078-8
更新日期：2005-06-01 00:00:00
abstract：:Cells binding anti-bovine TASH beta serum were found exclusively in the rostral lobe of the adenohypophysis of the drake using the peroxidase-antiperoxidase complex unlabelled antibody method. The specificity of the binding of the anti-serum to TSH cells was established by relating the morphology and relative abundanc...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234834
更新日期：1979-04-30 00:00:00
abstract：:Unfertilized eggs commonly lack centrioles, which are usually provided by the male gamete at fertilization, and are unable to assemble functional reproducing centrosomes. However, some insect species lay eggs that develop to adulthood without a contribution from sperm. We report that the oocyte of the parthenogenetic ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0253-x
更新日期：2006-12-01 00:00:00
abstract：:The distribution of the molluscan cardioexcitatory tetrapeptide FMRFamide (Phe-Met-Arg-Phe-NH2) in the brain of the cloudy dogfish, Scyliorhinus torazame, was examined by immunocytochemistry. FMRFamide-like immunoreactivity was demonstrated to occur extensively in various regions of the dogfish brain, except for the c...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00398072
更新日期：1991-08-01 00:00:00
abstract：:While brain-derived neurotrophic factor (BDNF) messenger RNA (mRNA) has been localized in the developing gustatory epithelium, little information is available about neurotrophin-3 (NT-3) mRNA expression pattern in the prenatal developing gustatory and lingual epithelium. In the present study, using in situ hybridizati...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051092
更新日期：1998-06-01 00:00:00
abstract：:Tenidap is a structurally novel antirheumatic agent with anti-inflammatory and analgesic properties. Previous studies have shown that tenidap is able to inhibit the production and action of cytokines such as interleukin-1, interleukin-6 (IL-6) and tumour necrosis factor alpha. However, the mechanisms by which tenidap ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050810
更新日期：1997-05-01 00:00:00
abstract：:Research in mammals has established the existence of a neuronal network that lies within the hypothalamus and that regulates energy homeostasis. However, it is unknown whether this system has been evolutionarily conserved. The objective of the present study was therefore to examine the influence of the agouti-related ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-003-0755-8
更新日期：2003-08-01 00:00:00
abstract：:Ionizing irradiation is an effective treatment for intracranial tumors but is limited by the potential adverse effects induced in surrounding normal brain. These effects can include cognitive impairments, and whereas the pathogenesis of such injury has not yet been definitively established, it may involve injury to th...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-007-0480-9
更新日期：2008-01-01 00:00:00
abstract：:The protein synthesis inhibitor cycloheximide (Chx) suppresses prolactin-induced beta-casein gene expression in the mammary epithelial cell line COMMA-D. As the mechanism underlying this effect is unclear, the effects of protein synthesis inhibitors on interactions of transcription factors with the beta-casein promote...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0672-2
更新日期：2003-02-01 00:00:00
