Abstract：
:A technique has been developed to obtain viable, isolated and enriched populations of gastrin cells (G-cells) from the rat stomach. Restricted tissue samples from a small area of the pyloric antrum known to be particularly rich in G-cells, were sequentially digested with pronase followed by mechanical agitation, to remove the epithelial cells. This technique resulted in a significant enrichment of G-cells (3-4 fold) since the surface epithelial cells and upper portions of the glands were discarded before the initial G-cell fraction was collected. These cells in suspension were then isolated from each other by gentle pipetting in a DNase containing solution and designated the crude preparation (CP). The G-cells were then purified further by separating the cells according to size by velocity sedimentation. The greatest concentration of G-cells (15-25%) was found in the fraction containing cells with diameters of 10 to 12 micrometer. The effectiveness of the technique was evaluated by counting G-cells as identified by electron microscopy and immunofluorescence and assessing gastrin activity by radioimmunoassay. All three methods indicated that cell separation by gravity velocity sedimentation enriched the G-cell population 15-20 fold over their concentration in the CP. The combined techniques of selective pronase digestion followed by gravity velocity sedimentation resulted in an isolated cell preparation containing a 50-100 fold increase of G-cells over their normal distribution in the intact gastric mucosa. Since these isolated G-cells retain features indicating viability, their usefulness for in vitro studies is suggested.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Forssmann WG,Lichtenberger LM,Helmstaedter V,Ito Sdoi
10.1007/BF00236409subject
Has Abstractpub_date
1979-08-01 00:00:00pages
163-77issue
2eissn
0302-766Xissn
1432-0878journal_volume
200pub_type
杂志文章abstract：:The distribution of somatostatin and cocaine and amphetamine-regulated transcript (CART) was investigated in rat intracardiac ganglia. Somatostatin immunoreactivity was only present in nerve terminals, always colocalised with choline acetyltransferase immunoreactivity, surrounding approximately 10% of intracardiac neu...
journal_title：Cell and tissue research
pub_type： 杂志文章
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abstract：:The Sgcb-null mouse, with knocked-down β-sarcoglycan, develops severe muscular dystrophy as in type 2E human limb girdle muscular dystrophy. The mdx mouse, lacking dystrophin, is the most used model for Duchenne muscular dystrophy (DMD). Unlike DMD, the mdx mouse has mild clinical features and shows little fibrosis in...
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：1980-01-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1979-06-27 00:00:00
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更新日期：1994-04-01 00:00:00
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更新日期：1999-09-01 00:00:00
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：1978-10-06 00:00:00
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journal_title：Cell and tissue research
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更新日期：1981-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：1981-01-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1997-01-01 00:00:00
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更新日期：2013-06-01 00:00:00
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