Recognition of sodium- and potassium-dependent adenosine triphosphatase in organs of the mouse by means of a monoclonal antibody.

Abstract:

:The antigen detected by the rat anti-mouse monoclonal antibody (m Ab), anti-BSP-3, has been initially described as a brain cell-surface protein. Evidence is presented that this m Ab recognizes mouse (Na+ + K+)-ATPase (ATP phosphohydrolase, E.C.3.6.1.3). The antigen, purified from mouse brain by means of affinity chromatography, migrated in SDS-polyacrylamide gels in the form of two polypeptide chains of 100 000 and 48 000 molecular weight, which could be shown to react with subunit-specific polyclonal antisera against ATPase in immunoblotting experiments. Purified BSP-3 antigen was bound to the specific (Na+ + K+)-ATPase inhibitor ouabain. Finally, the anti-BSP-3 m Ab was capable of immunoprecipitating the ATPase activity of a microsomal fraction from mouse kidney. The m Ab was used to study the localization of (Na+ + K+)-ATPase in different organs of the mouse. It stained the basolateral plasma membranes of polarized cells in immunofluorescence experiments, while the entire cell surface of unpolarized cells was labeled. Interestingly, several cell types did not react with the m Ab, indicating a possible heterogeneity of ATPases. Such a m Ab could prove to be a useful tool for studying localization, structure and function of (Na+ + K+)-ATPase.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Gorvel JP,Liabeuf A,Massey D,Liot D,Goridis C,Maroux S

doi

10.1007/BF00218655

subject

Has Abstract

pub_date

1983-01-01 00:00:00

pages

619-32

issue

3

eissn

0302-766X

issn

1432-0878

journal_volume

234

pub_type

杂志文章
  • Galanin-immunoreactive neurons in the guinea-pig small intestine: their projections and relationships to other enteric neurons.

    abstract::Galanin immunoreactivity was observed in nerve cell bodies and nerve fibres, but not in enteroendocrine cells, in the small intestine of the guinea-pig. Nerve terminals were found in the myenteric plexus, in the circular muscle, in submucous ganglia, around submucous arterioles, and in the mucosa. Lesion studies showe...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00218954

    authors: Furness JB,Costa M,Rökaeus A,McDonald TJ,Brooks B

    更新日期:1987-12-01 00:00:00

  • Interdigitating reticulum cells in the popliteal lymph node of the rat. An ultrastructural and cytochemical study.

    abstract::Electronmicroscopic and cytochemical studies were performed to localize interdigitating reticulum cells (IDC) in the popliteal lymph node of the rat. The morphological features of the IDC of the rat correspond to those described for other species, but also show similarities to normal macrophages in the rat. This is co...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00220109

    authors: Friess A

    更新日期:1976-07-20 00:00:00

  • Impaired healing of cornea incision injury in a TRPV1-deficient mouse.

    abstract::The present study attempts to elucidate the role of TRPV1 cation channel receptor on primary repair in an incision-wounded mouse cornea in vivo. Previous study revealed that blocking TRPV1 suppressed myofibroblast formation and expression of transforming growth factor β1 (TGFβ1) in cultured keratocytes or ocular fibro...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-018-2878-y

    authors: Nidegawa-Saitoh Y,Sumioka T,Okada Y,Reinach PS,Flanders KC,Liu CY,Yamanaka O,Kao WW,Saika S

    更新日期:2018-11-01 00:00:00

  • Immunocytochemical demonstration of met-enkephalin in the central nervous system of the domestic fowl.

    abstract::In the central nervous system of the male domestic flow, metenkephalin (ENK) immunoreactive perikarya and fiber tracts as well as extensive but sharply delimited fiber networks were visualized by means of the PAP technique. The most striking results were: (1) The demonstration of an association of ENK-containing struc...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00236920

    authors: Blähser S,Dubois MP

    更新日期:1980-01-01 00:00:00

  • Expression and cell-specific localization of the cholecystokinin B/gastrin receptor in the human stomach.

    abstract::Gastrin stimulates gastric acid secretion by acting on the cholecystokinin B/gastrin receptor (CCK-BR). The localization of this receptor at the cellular level showed conflicting results in animal studies and has not been described in man by immunohistochemistry. The aim of the present study is to characterize the pre...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004419900114

    authors: Kulaksiz H,Arnold R,Göke B,Maronde E,Meyer M,Fahrenholz F,Forssmann WG,Eissele R

    更新日期:2000-02-01 00:00:00

  • The major ganglion in the pelvic plexus of the male rat: a histochemical and ultrastructural study.

    abstract::To further evaluate the role of autonomic ganglia in the regulation of pelvic visceral activity, the neural elements in the major pelvic ganglion of the male rat have been studied with histochemixal and electron microscopic techniques. The principal findings are that the ganglion is composed of cholinergic and adrener...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00231994

    authors: Dail WG,Evan AP,Eason HR

    更新日期:1975-05-27 00:00:00

  • Neural pathways of olfactory kin imprinting and kin recognition in zebrafish.

    abstract::Teleost fish exhibit extraordinary cognitive skills that are comparable to those of mammals and birds. Kin recognition based on olfactory and visual imprinting requires neuronal circuits that were assumed to be necessarily dependent on the interaction of mammalian amygdala, hippocampus, and isocortex, the latter being...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s00441-020-03378-4

    authors: Gerlach G,Wullimann MF

    更新日期:2021-01-30 00:00:00

  • Trilayered tissue construct mimicking the orientations of three layers of a native heart valve leaflet.

    abstract::A tissue-engineered heart valve can be an alternative to a prosthetic valve in heart valve replacement; however, it is not fully efficient in terms of long-lasting functionality, as leaflets in engineered valves do not possess the trilayered native leaflet structure. Previously, we developed a flat, trilayered, orient...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-020-03241-6

    authors: Jana S,Lerman A

    更新日期:2020-11-01 00:00:00

  • The lack of terminal tubule cells in the submandibular gland of mice deficient in submandibular gland protein C.

    abstract::The submandibular gland (SMG) of newborn mice has no mature acini but has the rudiments of acini called terminal tubules (TT). The TT are composed of TT cells with dark secretory granules and proacinar cells with lighter secretory granules, the latter being considered the immediate precursor of mature acinar cells. TT...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-020-03205-w

    authors: Nakata H,Terakawa J,Horike SI,Daikoku T,Iseki S

    更新日期:2020-08-01 00:00:00

  • Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle.

    abstract::Mesenchymal stem cells (MSCs) are increasingly being reported as occurring in a variety of tissues. Although MSCs from human bone marrow are relatively easy to harvest, the isolation of rodent MSCs is more difficult, thereby limiting the number of experiments in vivo. To determine a suitable cell source, we isolated r...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-006-0308-z

    authors: Yoshimura H,Muneta T,Nimura A,Yokoyama A,Koga H,Sekiya I

    更新日期:2007-03-01 00:00:00

  • Wnt signalling in neuronal differentiation and development.

    abstract::Wnts are secreted glycoproteins that play multiple roles in early development, including the differentiation of precursor cells. During this period, gradients of Wnts and other morphogens are formed and regulate the differentiation and migration of neural progenitor cells. Afterwards, Wnt signalling cascades participa...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s00441-014-1996-4

    authors: Inestrosa NC,Varela-Nallar L

    更新日期:2015-01-01 00:00:00

  • The aminergic innervation of the pituitary gland in the roach Leuciscus rutilus.

    abstract::Fluorescent (aminergic) fibers were demonstrated by the Falck-Hillarp technique in all parts of the neurohypophysis (NH) in the roach (Leuciscus rutilus). The fibers are very thin and few in number in the rostral (RNH) and proximal neurohypophysis (PNH) but slightly more numerous in the NH adjacent to the pars interme...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00219959

    authors: Ekengren B

    更新日期:1975-01-01 00:00:00

  • Effects of light and dark on photoreceptors in the polychaete annelid Nereis limnicola.

    abstract::The effects of light and dark on photoreceptors of the brackish-water polychaete annelid Nereis limnicola were studied by electron microscopy. Animals dark-adapted for one or two days exhibited well-formed straight microvilli (rhabdomeres) on the sensory cell processes. Continuous illumination of worms for one or two ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00225427

    authors: Eakin RM,Brandenburger JL

    更新日期:1985-01-01 00:00:00

  • A study of scanning (SEM) and transmission (TEM) electron microscopy of the glomerular capillaries in developing rat kidney.

    abstract::Kidneys of 2 to 10 day-old rats of Wistar and Sprague-Dawley strains were fixed with glutaraldehyde by retrograde vascular perfusion and then prepared for observation in TEM and SEM. In addition methacrylate casts of differentiating glomerular capillaries were examined by SEM. Although the glomerular vascular pattern ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00233959

    authors: Kazimierczak J

    更新日期:1980-01-01 00:00:00

  • MyD88 hypermethylation mediated by DNMT1 is associated with LTA-induced inflammatory response in human odontoblast-like cells.

    abstract::Dental caries is a chronic, infectious, and destructive disease that allows bacteria to break into the dental pulp tissue. As caries-related bacteria invade the human dentinal tubules, odontoblasts are the first line of dental pulp that trigger the initial inflammatory and immune responses. DNA methylation is a key ep...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-019-02993-0

    authors: Meng R,Li D,Feng Z,Xu Q

    更新日期:2019-06-01 00:00:00

  • Ultrastructure of the new neuromuscular junctions formed during reinnervation of rat soleus muscle by a "foreign" nerve.

    abstract::In rats the "fast" fibular nerve was transposed to the "slow" soleus muscle outside the original innervation band. Formation of new neuromuscular junctions was induced by cutting the soleus nerve after different periods of time. The morphological maturation of these junctions was studied by electron microscopy. New ne...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00215176

    authors: Korneliussen H,Sommerschild H

    更新日期:1976-04-09 00:00:00

  • Enzyme histochemical characteristics of osteoblasts and mononucleated osteoclasts in a teleost fish with acellular bone (Oreochromis niloticus, Cichlidae).

    abstract::Bone resorption by mononucleated cells was studied in the acellular bone of a teleost fish (Oreochromis niloticus) by histological and enzyme histochemical observations and by transmission electron microscopy. Bone resorbing cells (osteoclasts) were identified by their location at the sites of bone resorption, their f...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410050782

    authors: Witten PE,Bendahmane M,Abou-Haila A

    更新日期:1997-02-01 00:00:00

  • Wnt5a plays a crucial role in determining tooth size during murine tooth development.

    abstract::We have previously demonstrated that tooth size is determined by dental mesenchymal factors. Exogenous bone morphogenetic protein (BMP)4, Noggin, fibroblast growth factor (FGF)3 and FGF10 have no effect on tooth size, despite the expressions of Bmp2, Bmp4, Fgf3, Fgf10 and Lef1 in the dental mesenchyme. Among the wingl...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-011-1224-4

    authors: Cai J,Mutoh N,Shin JO,Tani-Ishii N,Ohshima H,Cho SW,Jung HS

    更新日期:2011-09-01 00:00:00

  • Epithelial differentiation and taste buds in the soft palate of the monkey, Macaca irus.

    abstract::A combination of light, transmission and scanning electron microscopy was employed to demonstrate the occurrence, arrangement and structure of taste buds in the oral mucosa of the soft palate of monkeys (Macaca irus). Taste buds are found in aggregates confined to 0.15 to 0.3 mm wide, round islands of keratinizing epi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00236359

    authors: Klein PB,Schroeder HE

    更新日期:1979-01-30 00:00:00

  • Absence of the myelin-associated glycoprotein (MAG) and the neural cell adhesion molecule (N-CAM) interferes with the maintenance, but not with the formation of peripheral myelin.

    abstract::We have previously shown that mice deficient in the gene for the myelin-associated glycoprotein (MAG) develop normal myelin in the peripheral nerves, but show axon and myelin degeneration at eight months of age, suggesting that MAG is involved in the maintenance of axon-Schwann cell integrity. The search for molecules...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410050727

    authors: Carenini S,Montag D,Cremer H,Schachner M,Martini R

    更新日期:1997-01-01 00:00:00

  • Specialized contacts between sarcolemma and sarcoplasmic reticulum at the ends of muscle fibers in the diaphragm of the rat.

    abstract::An electron-microscopic study of the myotendinous portion of the diaphragm in the Wistar rat has shown that at the ends of muscle fibers, longitudinally oriented invaginations and peripheral furrows of the sarcolemma establish specialized contacts with individual sacs of the sarcoplasmic reticulum. The construction of...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00251058

    authors: Andreev DP,Wassilev WA

    更新日期:1986-01-01 00:00:00

  • Morphology and distribution of supraependymal cells in the third ventricle of the albino rat.

    abstract::The supraependymal cells (SEC) are a normal component of the wall of the cerebral ventricles. In the hypothalamic area of the third ventricle they are restricted, in healthy animals, to the ependymal projection of the hypophyseotropic area. Here the SEC show great polymorphism. In addition to bipolar, multipolar and s...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00215308

    authors: Mestres P,Breipohl W

    更新日期:1976-05-13 00:00:00

  • The contractile apparatus of striated muscle in the course of atrophy and regeneration. I. Myosin and actin filaments in the denervated rat soleus.

    abstract::The ultrastructure of the contractile apparatus of the rat soleus muscle during the course of denervation atrophy was investigated. It was found that the ratio of thin the thick filaments increased in myofibrils of atrophying muscle fibers. Elevation of the ratio was observed as early as the second day after denervati...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00216767

    authors: Jakubiec-Puka A,Kulesza-Lipka D,Krajewski K

    更新日期:1981-01-01 00:00:00

  • Uptake and degradation of radioactively labelled albumin microspheres as markers for Kupffer cell phagocytosis.

    abstract::Rats were injected with liposomes containing iodixanol (CTP10 Injection; 100 mg iodine per kg body weight) followed by a second injection of 125I-tyramine-cellobiose-albumin microspheres. The amounts of phagocytosed and degraded labelled albumin in liver were measured. A reduced uptake and degradation of albumin micro...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410000229

    authors: Kindberg GM,Tolleshaug H,Skotland T

    更新日期:2000-06-01 00:00:00

  • Localization of myosin and actin in ocular nonmuscle cells. Immunofluorescence-microscopic, biochemical, and electron-microscopic studies.

    abstract::Myosin and actin were localized by indirect immunofluorescence microscopy using specific antibodies prepared in rabbits against highly purified gizzard myosin and actin. A strong fluorescence staining with both antibodies was observed in rat corneal epithelial cells, anterior lens epithelial cells, rod inner segments,...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00221771

    authors: Drenckhahn D,Gröschel-Stewart U

    更新日期:1977-07-19 00:00:00

  • Long-term staining of live Merkel cells with FM dyes.

    abstract::Live Merkel cells in the skin and hair follicles are known to incorporate a fluorescence dye, quinacrine, which has been utilized to identify and dissect the cells for experiments. Quinacrine fluorescence of the cells is, however, quickly lost and quinacrine-stained Merkel cells soon become difficult to identify in ti...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-002-0688-7

    authors: Fukuda J,Ishimine H,Masaki Y

    更新日期:2003-03-01 00:00:00

  • Distribution of LHRH in the rat and mouse brain with special reference to the tanycytes.

    abstract::The distribution of luteinizing hormone-releasing hormone (LHRH) was studied in the rat and mouse brain by means of light and electron microscopic immunohistochemistry using the peroxidase-antiperoxidase method. An immunoreactive product to LHRH antiserum was found near the blood vessels of the vascular organ of the l...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00233914

    authors: Nozaki M,Taketani Y,Minaguchi H,Kigawa T,Kobayashi H

    更新日期:1979-03-19 00:00:00

  • Adrenocortical atrophy of hypophysectomized rats can be reduced by corticotropin-releasing hormone (CRH).

    abstract::The effects of high dose injections of corticotropin-releasing hormone (CRH) on the adrenal cortex of hypophysectomized rats were studied at the light- and electron-microscopical levels. Adrenocortical atrophy induced by hypophysectomy could be reduced by daily i.p. injection of 10 micrograms (3 nmol) CRH given for 3 ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00297501

    authors: Bornstein SR,Ehrhart M,Scherbaum WA,Pfeiffer EF

    更新日期:1990-04-01 00:00:00

  • Peripheral and central autonomic nervous system: does the sympathetic or parasympathetic nervous system bear the brunt of the pathology during the course of sporadic PD?

    abstract::It is a well-established fact that the sympathetic, parasympathetic and enteric nervous systems are affected at early stages in Parkinson's disease (PD). However, it is not yet clarified whether the earliest pathological events preferentially occur in any of these three divisions of the autonomic nervous system (ANS)....

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s00441-018-2851-9

    authors: Orimo S,Ghebremedhin E,Gelpi E

    更新日期:2018-07-01 00:00:00

  • Quantitative analysis of enteric neurons containing choline acetyltransferase and nitric oxide synthase immunoreactivities in the submucosal and myenteric plexuses of the porcine colon.

    abstract::The enteric nervous system (ENS) controls gastrointestinal functions. In large mammals' intestine, it comprises an inner (ISP) and outer (OSP) submucous plexus and a myenteric plexus (MP). This study quantifies enteric neurons in the ISP, OSP, and MP of the pig ascending (AC) and descending colon (DC) using the HuC/D,...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-020-03286-7

    authors: Mazzoni M,Caremoli F,Cabanillas L,de Los Santos J,Million M,Larauche M,Clavenzani P,De Giorgio R,Sternini C

    更新日期:2020-09-23 00:00:00