Oxytocin in Leydig cells: an immunocytochemical study of Percoll-purified cells from rat testes.

Abstract:

:Testicular interstitial cells, from rats aged 35 days, were dispersed with collagenase and separated through Percoll into 5 fractions (I-V); fraction I being the least dense. Measurement of basal testosterone production, histo-enzymological staining for 3 beta-hydroxysteroid dehydrogenase activity and electron microscopy indicated that the majority of Leydig cells were found in fraction IV (corresponding to a density of 1.076-1.097 g/ml). In addition, cells from this fraction responded to hCG treatment in a dose-dependent manner on day 0 and remained responsive after being cultured for 1 day. Immunostaining for oxytocin indicated that this fraction also contained the majority of the oxytocin-immunoreactive cells. On day 1 of culture, 56% of the cell population from fraction IV were positively stained for the steroidogenic enzyme and 75% immunoreactive for oxytocin. This overlap indicates that the Leydig cells were also the oxytocin immunoreactive cells.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Yeung WS,Guldenaar SE,Worley RT,Humphrys J,Pickering BT

doi

10.1007/BF00222304

subject

Has Abstract

pub_date

1988-08-01 00:00:00

pages

463-8

issue

2

eissn

0302-766X

issn

1432-0878

journal_volume

253

pub_type

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