Abstract：
:Pituitaries from normal, young and adult male rats were fixed either in sublimate-formalin or in glutaraldehyde-osmium. In adjacent Paraplast sections, almost all the gonadotrophs were immunostained with both LH and FSH antisera. The rat LH beta and FSH antisera used were shown to be highly specific by the absorption test and by double antibody radioimmunoassay. Thin and thick adjacent Epon sections were prepared for EM and immunohistochemical examination. Cells stained with the rat LH beta antiserum were identified by LM, and the observed in detail by EM. On the basis of these observations we suggest that the LH cells are arranged in a sequence of basophils, i.e., Types II/III, III, III/IV and IV: Type II/III basophils are elongate with a cytoplasmic process and less vesiculated. They have morphological features of Type II (classical thyrotrophs) and also of Type III basophils. Type III basophils are oval in shape and moderately vesiculated. Both Types II/III and III basophils can be divided into two classes of cell characterized mainly by the existence of only small secretory granules (150-220 nm in diameter) (Type A) or by the coexistence of small and large (350-500 nm) (Type B). Type III/IV basophils are cells intermediate between types III and IV basophils, and moderately vesiculated with an abundance of secretory granules (150-300 nm in diameter). Type IV basophils are large, spherical or oval cells whose RER cisternae are conspicuously dilated; they contain less numerous secretory granules (150-300 nm in diameter). It is concluded that LH cells are not a single cell type, but include a wide range of subtypes.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Yoshimura F,Nogami H,Shirasawa N,Yashiro Tdoi
10.1007/BF00233820subject
Has Abstractpub_date
1981-01-01 00:00:00pages
1-10issue
1eissn
0302-766Xissn
1432-0878journal_volume
217pub_type
杂志文章abstract：:Eosinophils are innate immune leukocytes found in relatively low numbers within the blood. Terminal effector functions of eosinophils, deriving from their capacity to release their content of tissue-destructive cationic proteins, have historically been considered primary effector mechanisms against specific parasites,...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-010-1049-6
更新日期：2011-01-01 00:00:00
abstract：:The two calcium antagonistic agents lanthanum and tetracaine cause severe disturbances in the secretory process of the exocrine pancreas, including inhibition of the rate of protein synthesis and exocytosis. The former effect resulted mainly from the inhibition of amino acid transport. Lanthanum in a concentration up ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00227029
更新日期：1977-05-10 00:00:00
abstract：:The medial preoptic nucleus of the Japanese quail is a testosterone-sensitive structure that is involved in the control of male copulatory behavior. The full understanding of the role played by this nucleus in the control of reproduction requires the identification of its afferent and efferent connections. In order to...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00343944
更新日期：1994-06-01 00:00:00
abstract：:We report here on cholinergic neurons in the rat hippocampal formation that were identified by immunocytochemistry employing a monoclonal antibody against choline acetyltransferase (ChAT), the acetylcholine-synthesizing enzyme. In general, ChAT-immunoreactive cells were rare, but were observed in all layers of the hip...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215891
更新日期：1986-01-01 00:00:00
abstract：:Electronmicroscopic and cytochemical studies were performed to localize interdigitating reticulum cells (IDC) in the popliteal lymph node of the rat. The morphological features of the IDC of the rat correspond to those described for other species, but also show similarities to normal macrophages in the rat. This is co...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220109
更新日期：1976-07-20 00:00:00
abstract：:Honeybees are extensively used to study olfactory learning and memory processes thanks to their ability to discriminate and remember odors and because of their advantages for optophysiological recordings of the circuits involved in memory and odor perception. There are evidences that the encoding of odors in areas of ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-020-03396-2
更新日期：2021-01-29 00:00:00
abstract：:Hepatic stellate cells become activated and aquire contractility on being cultured. In order to characterize the morphology of contracted and relaxed stellate cells, we performed light- and electron-microscopic analyses of cultured stellate cells on collagen gels. Incubation of stellate cells with medium alone, 10 nM ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050717
更新日期：1996-12-01 00:00:00
abstract：:This study describes the morphology and distribution of setae on the lateral and medial flagella of the antennules of the spiny lobster Panulirus argus in an effort to identify antennular chemoreceptors in addition to the well-studied aesthetasc chemosensilla. Setae were examined using light and electron microscopy, a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100377
更新日期：2001-06-01 00:00:00
abstract：:Periodontal ligament (PDL) stem cell properties are critical in the periodontal tissue regeneration for periodontitis. Previously, we have demonstrated that cigarette smoking attenuates PDL-derived stem cell (PDLSC) regenerative properties. Here, we report the findings on the regenerative properties of human PDLSCs wi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03176-y
更新日期：2020-07-01 00:00:00
abstract：:Thyroid glands of 7 to 21 day-old chick embryos were examined by electron microscopy using freeze-fracture and thin-section preparations. The primitive follicle lumen first appears between two adjacent epithelial cells in 8 day-old embryos, and is formed in the region of a focal tight junction (macula occludens). The ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234830
更新日期：1979-04-30 00:00:00
abstract：:In contrast to the vitellolysis zone which is involved in the degradation of the yolk, the cytoplasmic zone of the yolk syncytial layer, composed of many cytoplasmic organelles, is implicated in a secretory process. The granular endoplasmic reticulum of the latter is extremely well developed and organized into trabecu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236349
更新日期：1979-01-30 00:00:00
abstract：:The gene encoding H1t, a testicular variant of histone H1, is expressed in mammals during spermatogenesis. Northern blot and in situ hybridization has detected H1t mRNA only at the stage of pachytene spermatocytes. We have extended this analysis to more sensitive approaches and demonstrate, by RNase protection and ele...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050986
更新日期：1998-01-01 00:00:00
abstract：:Taste buds and the peripheral nerves innervating them are two important components of the peripheral gustatory system. They require appropriate connections for the taste system to function. Neurotrophic factors play crucial roles in the innervation of peripheral sensory organs and tissues. Both brain-derived neurotrop...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-009-0833-7
更新日期：2009-09-01 00:00:00
abstract：:Neurofilaments, which are exclusively found in nerve cells, are one of the earliest recognizable features of the maturing nervous system. The differential distribution of neurofilament proteins in varying degrees of phosphorylation within a neuron provides the possibility of selectively demonstrating either somata and...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051013
更新日期：1998-03-01 00:00:00
abstract：:This is the first detailed report about the collar enamel of the teeth of Polypterus senegalus. We have examined the fine structure of the collar enamel and enamel organ of Polypterus during amelogenesis by light and transmission electron microscopy. An immunohistochemical analysis with an antibody against bovine amel...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1305-4
更新日期：2012-02-01 00:00:00
abstract：:Chorionic binucleate cells (BNC) occur in several ruminants including cow, deer, goat and sheep. They migrate through the chorionic tight junction to fuse with uterine epithelial cells and discharge their granules into maternal connective tissue. We have compared the BNC of near-term, resin-embedded, ovine and bovine ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00331380
更新日期：1994-12-01 00:00:00
abstract：:We have tested the suggestion that the reported increase, in hypoxic rats, in the number of lung endocrine cells immunoreactive for the regulatory peptide CGRP is caused by an accumulation of peptide within the cells which renders them more detectable, rather than by a real increase in proliferation. The incorporation...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00338049
更新日期：1992-04-01 00:00:00
abstract：:Nervous elements in the periodontal ligament and dental pulp of rat incisors were investigated by means of immunohistochemistry for neurofilament protein (NFP) and glia-specific S-100 protein. The periodontal ligament in the incisors was densely innervated by NFP-immunoreactive nerve fibers; the distribution of the ne...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215442
更新日期：1988-01-01 00:00:00
abstract：:We have previously shown that mice deficient in the gene for the myelin-associated glycoprotein (MAG) develop normal myelin in the peripheral nerves, but show axon and myelin degeneration at eight months of age, suggesting that MAG is involved in the maintenance of axon-Schwann cell integrity. The search for molecules...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050727
更新日期：1997-01-01 00:00:00
abstract：:A somatostatin-like substance is demonstrated by light microscopic immunohistochemistry (PAP-method) in perikarya and cell processes of the retina of adult and infant rats. These perikarya are identified according to their size, arrangement and distribution. Each of the first two neuronal orders (receptors, bipolar ce...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00235169
更新日期：1979-01-01 00:00:00
abstract：:Digital dermatitis (DD), a common ulcerative disease of the bovine foot causing lameness and reducing productivity and animal welfare, is associated with infection by spirochete Treponema bacteria. Topical tetracycline, the most common treatment, has inconsistent cure rates; therefore, new therapeutic options are need...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-03082-y
更新日期：2020-02-01 00:00:00
abstract：:Corneal fibroblasts, major cellular components of the corneal stroma, are loosely arrayed between collagen lamellae. They play an important role in the metabolic and physiological homeostasis mechanisms by which the cornea is kept transparent. This paper deals with the demonstration of the gap junctions between the co...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215533
更新日期：1987-08-01 00:00:00
abstract：:With the aid of electron microscopic immunocytochemistry following the application of antisera against somatostatin and luliberin (LRF), a labeling of the intercellular clefts in different areas of the brain was observed. This labeling is especially conspicuous near the basal pose of the cuboidal ependymal cells, but ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236448
更新日期：1980-01-01 00:00:00
abstract：:The purpose of this study was to determine the cellular distribution and degradation in rat liver following intravenous injection of superparamagnetic iron oxide nanoparticles used for magnetic resonance imaging (NC100150 Injection). Relaxometric and spectrophotometric methods were used to determine the concentration ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0884-8
更新日期：2004-06-01 00:00:00
abstract：:Rat blood monocytes contain granules of two types, primary and secondary granules, which can be distinguished on morphological grounds. Secondary granules have a halo between the limiting membrane and the matrix; primary granules have no halo and are generally more elongate than the secondary granules. In addition, th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00226665
更新日期：1977-11-30 00:00:00
abstract：:In marine cartilaginous fish, reabsorption of filtered urea by the kidney is essential for retaining a large amount of urea in their body. However, the mechanism for urea reabsorption is poorly understood due to the complexity of the kidney. To address this problem, we focused on elephant fish (Callorhinchus milii) fo...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-015-2234-4
更新日期：2015-12-01 00:00:00
abstract：:The dissociation and maintenance in culture of cells derived from the mushroom bodies of adult crickets (Acheta domesticus) are described. This primary culture was developed in order to investigate maturation and differentiation of mushroom-body cells including Kenyon cells, the major intrinsic interneurons of mushroo...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051023
更新日期：1998-03-01 00:00:00
abstract：:Epidermal septate junctions of Nereis sp. and Cirriformia sp. fixed with OsO4 or glutaraldehyde/OsO4 display variable structure in electron micrographs. In transverse section the septa are often indistinct and obscured by opaque material that fills the junctional cleft. Septa (spaced at 180--280 A) are more clearly de...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222150
更新日期：1976-10-22 00:00:00
abstract：:We compare the ultrastructure of the gerbil sublingual gland as seen after cryofixation followed by substitution with osmium tetroxide, with the more familiar appearance of material processed by glutaraldehyde-osmium chemical fixation. After primary cryofixation of fresh salivary glands, the nuclei of the mucous cells...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219075
更新日期：1987-11-01 00:00:00
abstract：:Polysaccharides in the vacuolar system of an established line of monkey kidney epithelial cells, were investigated by high resolution radioautography following incorporation of 3H-fucose and by the periodic acid-thiosemicarbazide-silver proteinate staining method. Significant amounts of glycogen and glycoproteins were...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00225345
更新日期：1978-10-30 00:00:00
