Abstract：
:Neurofilaments, which are exclusively found in nerve cells, are one of the earliest recognizable features of the maturing nervous system. The differential distribution of neurofilament proteins in varying degrees of phosphorylation within a neuron provides the possibility of selectively demonstrating either somata and dendrites or axons. Non-phosphorylated neurofilaments typical of somata and dendrites can be visualized with the aid of monoclonal antibody SMI 311, whereas antibody SMI 312 is directed against highly phosphorylated axonal epitopes of neurofilaments. The maturation of neuronal types, the development of area-specific axonal networks, and the gradients of maturation can thus be demonstrated. Optimal immunostaining with SMI 311 and SMI 312 is achieved when specimens are fixed in a mixture of paraformaldehyde and picric acid for up to 3 days and sections are incubated free-floating. Neurons, with their dendritic domains immunostained by SMI 311 in a Golgi-like manner, can be completely visualized in relatively thick sections. The limitations of Golgi-preparations, such as glia-labeling, artifacts, and the staining of only a small non-representative percentage of existing neurons, are not apparent in SMI preparations, which additionally provide the possibility of selectively staining axonal networks. The results achieved in normal fetal brain provide the basis for studies of developmental disturbances.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Ulfig N,Nickel J,Bohl Jdoi
10.1007/s004410051013subject
Has Abstractpub_date
1998-03-01 00:00:00pages
433-43issue
3eissn
0302-766Xissn
1432-0878journal_volume
291pub_type
杂志文章abstract：:Nitric oxide and various neuropeptides in the myenteric plexus regulate esophageal motility. We sought colocalization of nitric oxide synthase and neuropeptides in frozen sections of mid-portion of smooth-muscled opossum esophagus using NADPH-diaphorase activity to mark the synthase and immunoreactivity to detect pept...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00331374
更新日期：1994-12-01 00:00:00
abstract：:Our previous findings have demonstrated that the rat autosomal-recessive mutation, whitish chalk-like teeth (wct), induces enamel defects resembling those of human amelogenesis imperfecta (AI) in continuously growing incisor teeth. The present study clarifies the effect of the wct mutation on the morphogenesis and cal...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0452-0
更新日期：2007-10-01 00:00:00
abstract：:Nucleoside di-phosphate kinase enzyme (NDPK) isoforms, encoded by the nm23 family of genes, may be involved in various cellular differentiation and proliferation processes. We have therefore analyzed the expression of nm23-M1, -M2, -M3, and -M4 during embryonic mouse development. In situ hybridization data has reveale...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0036-9
更新日期：2005-12-01 00:00:00
abstract：:We describe the histological basis of color metamorphosis in the polychromatic Midas cichlid, Cichlasoma citrinellum. Eight percent of the individuals in a natural population transform from gray with black markings to orange, simultaneously losing their ability to adjust coloration in response to background and social...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221733
更新日期：1988-07-01 00:00:00
abstract：:Semaphorin 3A (Sema3A) axon repellant serves multiple developmental functions. Sema3A mRNAs are expressed in epithelial and mesenchymal components of the developing incisor in a dynamic manner. Here, we investigate the functions of Sema3A during development of incisors using Sema3A-deficient mice. We analyze histomorp...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-1839-3
更新日期：2014-07-01 00:00:00
abstract：:The formation of secretory granules in chronically hypersecretory melanotrophs in the rat pituitary was studied. Hypersecretion was induced by treatment with the dopamine antagonist haloperidol (1.5 mg/kg daily for 7 days), which releases the normal neural dopaminergic inhibition of secretion from the melanotroph. Mor...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00319432
更新日期：1994-02-01 00:00:00
abstract：:Interstitial cells of Cajal (ICCs) are believed to be a major element in generating the spontaneous rhythm of the gastrointestinal tract. A prominent problem in the study of these cells has been the difficulty in observing them in intact tissues. We used the lipophilic dye DiI to stain ICCs in the submucosal-circular ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051058
更新日期：1998-05-01 00:00:00
abstract：:Kidneys of 2 to 10 day-old rats of Wistar and Sprague-Dawley strains were fixed with glutaraldehyde by retrograde vascular perfusion and then prepared for observation in TEM and SEM. In addition methacrylate casts of differentiating glomerular capillaries were examined by SEM. Although the glomerular vascular pattern ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233959
更新日期：1980-01-01 00:00:00
abstract：:The effect of phenylthiourea (PTU) on the pigment epithelium and the photoreceptor cells in the developing retina of Haplochromis burtoni was studied by electron microscopy. In the retinal pigment epithelium of 6-day old embryos, both types of melanin granule (spindle-shaped and rod-shaped) are already found. PTU inhi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219054
更新日期：1977-03-09 00:00:00
abstract：:Thin sections after bile duct ligation showed that the depth of tight junctions appeared to increase and that the distance between individual punctate contacts appeared to become irregular and wider than in controls. The freeze fracture replicas clearly demonstrated these changes in the tight junction morphology. Chan...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236724
更新日期：1978-12-28 00:00:00
abstract：:New genome-editing approaches, such as the CRISPR/Cas system, have opened up great opportunities to insert or delete genes at targeted loci and have revolutionized genetics in model organisms like the zebrafish. The Cre-loxp recombination system is widely used to activate or inactivate genes with high spatial and temp...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2798-x
更新日期：2018-04-01 00:00:00
abstract：:Hypothalamic slices containing the lateral hypothalamic area (LHA) were prepared from 6- to 8-day-old rats and maintained in stationary culture for up to 35 days in order to analyse how well the melanin-concentrating hormone (MCH) neurons survived. As previously reported for other brain areas, this method yielded a lo...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051330
更新日期：1999-07-01 00:00:00
abstract：:Enterochromaffin (EC) cells regulate gut motility and secretion in response to luminal stimuli, via the release of serotonin (5-HT). Inflammatory bowel disease and other gastrointestinal disorders are associated with increased numbers of EC cells and 5-HT availability. Our aim was to determine whether proliferation of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0430-6
更新日期：2007-09-01 00:00:00
abstract：:Early diplotene oocytes from Necturus maculosus ranging from approximately 0.2 to 0.5 mm in diameter were examined by electron microscopy. In the smallest oocytes of this range, the cytoplasm is largely devoid of membranes, but contains primarily ribosomes and mitochondria. In slightly larger oocytes, smooth-surfaced ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218087
更新日期：1986-01-01 00:00:00
abstract：:In the corpuscles of Stannius of sticklebacks and eels two cell types are described of presumably endocrine nature. The predominating type, comparable to the cells observed in other species, responds to variation in calcium content of the medium and possibly produces a hypocalcemic hormone. The second cell type is unr...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219163
更新日期：1975-06-09 00:00:00
abstract：:Pituitary adenylate cyclase-activating polypeptide (PACAP) and helospectin are two vasoactive intestinal polypeptide (VIP)-related neuropeptides that have recently been demonstrated in the mammalian gut; the aim of this study was to reveal their occurrence and localisation in the gastrointestinal tract, swimbladder, u...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00300227
更新日期：1994-09-01 00:00:00
abstract：:This is the first detailed report about the collar enamel of the teeth of Polypterus senegalus. We have examined the fine structure of the collar enamel and enamel organ of Polypterus during amelogenesis by light and transmission electron microscopy. An immunohistochemical analysis with an antibody against bovine amel...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1305-4
更新日期：2012-02-01 00:00:00
abstract：:Obesity induces metabolic disorders such as type 2 diabetes, hypertension, and cardiovascular diseases and has become a global health concern. Recent studies imply that fat accumulation in nonadipose tissue correlates with metabolic disorders. However, there are no suitable animal models to evaluate this phenomenon. T...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2908-9
更新日期：2019-02-01 00:00:00
abstract：:The nine-banded armadillo possesses a salivary bladder which is a dilated portion of the main duct of the submandibular gland at its origin. The wall of the bladder is composed of an epithelium, a submucosa and a thick coat of skeletal muscle. The ultrastructure of the epithelium reveals that it is complex and consist...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219609
更新日期：1976-06-28 00:00:00
abstract：:Calbindin is a calcium-binding protein, commonly found in certain subpopulations of the enteric nervous system in mammals. Recently, calbindin-immunoreactive enteric neurons have also been demonstrated in shorthorn sculpin (Myoxocephalus scorpius). In the present study, calbindin immunoreactivity has been investigated...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1135-4
更新日期：2011-04-01 00:00:00
abstract：:This report demonstrates that the administration of testosterone (T) or 11-ketotestosterone (11-KT) to sexually immature (8 wks old) male platyfish (Xiphophorus maculatus) of early- and late-maturing genotypes affects the synthesis and/or release of luteinizing hormone-releasing hormone (LHRH), as assessed by immunocy...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218552
更新日期：1986-01-01 00:00:00
abstract：:In the central nervous system of the male domestic flow, metenkephalin (ENK) immunoreactive perikarya and fiber tracts as well as extensive but sharply delimited fiber networks were visualized by means of the PAP technique. The most striking results were: (1) The demonstration of an association of ENK-containing struc...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236920
更新日期：1980-01-01 00:00:00
abstract：:In this study, we demonstrate the role of M cells in uptake of poly(D-L-lactic-co-glycolic acid) (PLGA) microspheres and transport into rabbit Peyer's patches. Microspheres 1 to 10 microns in diameter composed of 50:50 lactic acid:glycolic acid were instilled into intestinal segments containing jejunal or ileal Peyer'...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318501
更新日期：1995-02-01 00:00:00
abstract：:The pineal organ of the blind, cave-dwelling fish, Typhlichythyes subterraneous, was examined with both light and electron microscopes. Like the eyes, the pineal in this troglobytic species was found to be regressed. Two cell types, photoreceptor and supportive cells, were described in the pineal epithelium. Although ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233895
更新日期：1978-12-29 00:00:00
abstract：:The passage of cells across the lymphatic endothelium of rat lacteals in both normal and non-pathological experimental conditions (fasting, lymphatic, stasis) was studied by means of serial thin sections and three-dimensional models. Two different pathways of transendothelial migration were observed: (1) macrophages e...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327761
更新日期：1990-10-01 00:00:00
abstract：:The blood-brain barrier (BBB) protects the brain from toxic substances within the bloodstream and keeps the brain's homeostasis stable. On the other hand, it also represents the main obstacle in the treatment of many CNS diseases. Among different techniques, nanoparticles have emerged as promising tools to enhance bra...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-014-1819-7
更新日期：2014-03-01 00:00:00
abstract：:In the present study, we used ultrastructural cytochemistry to analyze the distribution of nuclear and cytoplasmic nucleic acids and polysaccharides, and electron spectroscopic imaging to map the element phosphorus in immature erythroid cells taken from two amphibians, the diploid Bufo ictericus and the tetraploid Odo...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00305791
更新日期：1994-10-01 00:00:00
abstract：:The endoneurial collagen sheath around teased nerve fibres following crush injury was studied by scanning electron microscopy and compared with uninjured sciatic nerve fibres and with fibres from the dystonic mutant mouse. Following crush injury the endoneurial collagen became more abundant than seen in untreated nerv...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210469
更新日期：1981-01-01 00:00:00
abstract：:The thyroid cells of the cream hamster, characterized by abundance of microtubules and stratification of the organelles, undergo a particular evolution when the animals grow older. These changes are characterized by an increase of the number of lysosomes which in extreme cases become so prominent that they occupy the ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215124
更新日期：1976-02-06 00:00:00
abstract：:A study of the organisation of the locust dorsal ocellus shows that the structure is designed to provide the maximum possible effective aperture. The condenser-like cuticular lens and the dispersal of the rhabdome over a large proportion of the circumferential area of the retinula cells increases the light gathering p...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234742
更新日期：1979-02-28 00:00:00
