Abstract：
:Nitric oxide and various neuropeptides in the myenteric plexus regulate esophageal motility. We sought colocalization of nitric oxide synthase and neuropeptides in frozen sections of mid-portion of smooth-muscled opossum esophagus using NADPH-diaphorase activity to mark the synthase and immunoreactivity to detect peptides. The peptides, all with demonstrated physiological activity in this organ, were calcitonin gene-related peptide, galanin, neuropeptide Y, substance P, and vasoactive intestinal polypeptide. The ExtrAvidin Peroxidase immunostain for each peptide was carried up to the final peroxidase reaction with 3-amino-9-ethyl-carbazole. The NADPH-diaphorase reaction was applied with short incubation to provide light staining just before the peroxidase reaction was performed. We examined sections for the proportions of singly and dually labeled nerve cells in the myenteric plexus. NADPH-diaphorase activity was highly colocalized with calcitonin gene-related peptide (59%), galanin (54%), and vasoactive intestinal polypeptide (53%). It showed little colocalization with neuropeptide Y (10%) and substance P (8%). The proportions of all nerve cells containing each of the substances were: NADPH-diaphorase--33%, calcitonin gene-related peptide--30%, galanin--55%, neuropeptide Y--16%, substance P--35%, and vasoactive intestinal polypeptide--58%. We conclude that the nerves responsible for peristalsis in the esophagus may act by releasing nitric oxide along with other inhibitory substances, calcitonin gene-related peptide, galanin, and vasoactive intestinal polypeptide, but not excitatory substances, neuropeptide Y and substance P.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Christensen J,Fang Sdoi
10.1007/BF00331374subject
Has Abstractpub_date
1994-12-01 00:00:00pages
557-62issue
3eissn
0302-766Xissn
1432-0878journal_volume
278pub_type
杂志文章abstract：:The so-called 'basement membrane' of arthropod compound eyes is known to be of heterogeneous origin (Odselius and Eloffson 1981). A major contribution in Diptera with open rhabdoms is provided by a pigmented component which lies at the basal end of the extracellular space of each ommatidium and fills it, the glial plu...
journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：1982-01-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：2018-11-01 00:00:00
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：1995-06-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1996-05-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1985-01-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：2001-07-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1981-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
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更新日期：2006-11-01 00:00:00
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：1991-05-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1990-09-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1982-01-01 00:00:00
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journal_title：Cell and tissue research
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