Abstract:
:New genome-editing approaches, such as the CRISPR/Cas system, have opened up great opportunities to insert or delete genes at targeted loci and have revolutionized genetics in model organisms like the zebrafish. The Cre-loxp recombination system is widely used to activate or inactivate genes with high spatial and temporal specificity. Using a CRISPR/Cas9-mediated knock-in strategy, we inserted a zebrafish codon-optimized CreER T2 transgene at the otx2 gene locus to generate a conditional Cre-driver line. We chose otx2 as it is a patterning gene of the anterior neural plate that is expressed during early development. By knocking in CreER T2 upstream of the endogenous ATG of otx2, we utilized this gene's native promoter and enhancer elements to perfectly match CreER T2 and endogenous otx2 expression patterns. Next, by combining this novel driver line with a Cre-dependent reporter line, we show that only in the presence of tamoxifen can efficient Cre-loxp-mediated recombination be achieved in the anterior neural plate-derived tissues like the telencephalon, the eye and the optic tectum. Our results imply that the otx2:CreER T2 transgenic fish will be a valuable tool for lineage tracing and conditional mutant studies in larval and adult zebrafish.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Kesavan G,Hammer J,Hans S,Brand Mdoi
10.1007/s00441-018-2798-xsubject
Has Abstractpub_date
2018-04-01 00:00:00pages
41-50issue
1eissn
0302-766Xissn
1432-0878pii
10.1007/s00441-018-2798-xjournal_volume
372pub_type
杂志文章abstract::In three mature monkeys (Macaca fascicularis; 3.5 +/- 0.3 kg in weight), the labial, buccal and soft-palate mucosae were examined morphologically and stereologically. Using fixation by perfusion, standardized methods of tissue preparation and morphometric analysis at the light-microscopic level, the gross dimensions (...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00213740
更新日期:1984-01-01 00:00:00
abstract::We have developed a primary cell culture system of antennal mechanoreceptor neurons from early-stage pupal sphinx moth Manduca sexta. Dissociated neurons from the moth antennae differentiated, grew and survived for several weeks in a conditioned culture medium. Bipolar neurons with soma diameters of 10-25 microns from...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051358
更新日期:1999-08-01 00:00:00
abstract::Enzyme-histochemical methods were used to study the metabolic activity of specialized ependyma of the ventrolateral walls and floor of the third ventricle in young male and female rats during the "critical period" of sexual differentiation of the hypothalamus (one week after birth). Histochemical tests were conducted ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220328
更新日期:1976-10-13 00:00:00
abstract::We present an experimental method allowing the production of three-dimensional organ-like structures, namely microtissues (MTs), in vitro without the need for exogenous extracellular matrix (ECM) or growth factors. Submandibular salivary glands (embryonic day ED14), kidneys (ED13) and lungs (ED13) were harvested from ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-016-2487-6
更新日期:2016-12-01 00:00:00
abstract::Enterochromaffin (EC) cells regulate gut motility and secretion in response to luminal stimuli, via the release of serotonin (5-HT). Inflammatory bowel disease and other gastrointestinal disorders are associated with increased numbers of EC cells and 5-HT availability. Our aim was to determine whether proliferation of...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-007-0430-6
更新日期:2007-09-01 00:00:00
abstract::The ultrastructural localization of dipeptidyl peptidase IV (DPP IV) (EC 3.4.14.5) in rat submandibular and parotid glands was studied immunocytochemically by the peroxidase-antiperoxidase (PAP) method, using a monospecific antiserum against rat kidney DPP IV. There were no differences in the immunocytochemical locali...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217869
更新日期:1984-01-01 00:00:00
abstract::During normal development of many vertebrate species, substantial numbers of neurons in the central and peripheral nervous system undergo naturally occurring (or programmed) cell death. For example, approximately 50% of spinal motoneurons degenerate and die at a time when these cells are establishing synaptic connecti...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050690
更新日期:1996-11-01 00:00:00
abstract::The development of the rat adrenal medulla was studied at the ultrastructural level with particular emphasis placed on early discrimination of different catecholamine-storing cells. The first granule-containing cells, phaeochromoblasts, were seen at day 15 of gestation migrating into the anlage of the cortex. These ce...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233834
更新日期:1981-01-01 00:00:00
abstract::The neuropeptide content of nerve fibers associated with submucosal arteries in the small intestine of guinea pigs was studied in whole-mount preparations using immunohistochemical methods. Tissues were obtained from normal animals or animals in which the small intestine had been extrinsically denervated. In normal an...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219756
更新日期:1988-09-01 00:00:00
abstract::The fine structural organization of a cortical filament layer in normal locomoting Amoeba proteus was demonstrated using improved fixation and embedding techniques. Best results were obtained after application of PIPES-buffered glutaraldehyde in connection with substances known to prevent the depolymerization of F-act...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215699
更新日期:1982-01-01 00:00:00
abstract::The localization of inhibin alpha-subunit in the human testis was studied at the light- and electron-microscope level with immunostaining techniques. Antibodies against specific fragments of porcine and human inhibin alpha-subunits were utilized. At light microscopy, inhibin alpha-subunit immunoreactivity was detected...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00319612
更新日期:1992-08-01 00:00:00
abstract::The structure of the bulbus arteriosus of a wide range of teleost fish is described with particular reference to the vascularization and innervation. The adventitia of the organ consists of blood vessels and large nerve bundles in a collagen matrix. The nerve bundles contain monoamines, and fluorescence studies show s...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00240106
更新日期:1979-02-15 00:00:00
abstract::Estradiol injections increase serum level of calcium, amino acid, glucose, protein, ammonia and creatinine in immature Epinephelus akaara, and also increase levels of total lipid, cholesterol, phospholipid and esterified fatty acids. Hepatic protein, glycogen and lipid concentrations also rise after estradiol treatmen...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217235
更新日期:1984-01-01 00:00:00
abstract::Somatostatin-like immunoreactivity was localized in nerve cell bodies and nerve terminals in the cat coeliac ganglion. Two types of somatostatin-immunoreactive cell bodies were revealed, the first being large (diameter 35 microns), numerous and weakly labelled, whereas the second was considerably smaller (diameter 10....
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00327290
更新日期:1991-03-01 00:00:00
abstract::The muscle cells of the ventricle, the branchial heart and the branchial heart appendages of Rossia macrosoma (Delle Chiaje) are studied. The ventricle myocardium has three muscle layers, while the other two organs exhibited a loose arrangement of muscle cells. The muscle cells of the ventricle, the branchial heart an...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:
更新日期:1977-12-13 00:00:00
abstract::Antithrombin (AT), which is mainly synthesized in the liver, is an acute-phase plasma protein in mammalian species. Here, we demonstrated that sheep anti-human AT antibody cross-reacted with the humoral fluids in amphioxus Branchiostoma belcheri tsingtauense as well as human serum. The concentration of AT in the humor...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-0088-x
更新日期:2006-03-01 00:00:00
abstract::Surface features of the ependymal linings of the fourth ventricle in the fowl were analyzed employing the scanning electron microscope (SEM). On the floor of the median sulcus, each ependymal cell has a solitary cilium, whereas on both sides of the sulcus, cilia are so densely distributed that the details of the under...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233562
更新日期:1979-03-09 00:00:00
abstract::Bone resorption by mononucleated cells was studied in the acellular bone of a teleost fish (Oreochromis niloticus) by histological and enzyme histochemical observations and by transmission electron microscopy. Bone resorbing cells (osteoclasts) were identified by their location at the sites of bone resorption, their f...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050782
更新日期:1997-02-01 00:00:00
abstract::Previous studies have identified Dogiel type II neurons with cell bodies in the myenteric plexus of guinea-pig ileum to be intrinsic primary afferent neurons. These neurons also have distinctive electrophysiological characteristics (they are AH neurons) and 82-84% are immunoreactive for calbindin. They are the only ca...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410100395
更新日期:2001-07-01 00:00:00
abstract::The glucose transporter 8 (GLUT8) is a recently identified member of the family of sugar transport facilitators. In human tissues GLUT8 is predominantly expressed in testis in a gonadotropin-dependent manner. It is shown here that the onset of mRNA synthesis of GLUT8 during the maturation of mouse testis coincides wit...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-001-0499-2
更新日期:2002-02-01 00:00:00
abstract::The ultrastructure of the optic and trigeminal nerves of the rat, cryofixed by use of a liquid nitrogen-propane jet, was examined, paying special attention to the myelin sheath and the cytoskeleton of the axoplasm. The cytoskeleton of the axoplasm is formed by a meshwork of neurofilaments and microtubules connected bo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214541
更新日期:1985-01-01 00:00:00
abstract::Epidermal septate junctions of Nereis sp. and Cirriformia sp. fixed with OsO4 or glutaraldehyde/OsO4 display variable structure in electron micrographs. In transverse section the septa are often indistinct and obscured by opaque material that fills the junctional cleft. Septa (spaced at 180--280 A) are more clearly de...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222150
更新日期:1976-10-22 00:00:00
abstract::The effect of pilocarpine and food uptake on the rate of incorporation of [3H]-leucine in vivo was measured by means of quantitative radioautography in three exocrine cells of the rat: the acinar and the granular duct cells of the submandibular and the acinar cells of the parotid gland. The three cell types react diff...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219936
更新日期:1975-12-18 00:00:00
abstract::Voltage-gated Na+ channels are expressed by highly metastatic MAT-LyLu cells, but not by poorly metastatic AT-2 cells, derived from the rodent Dunning model of prostatic cancer. We have investigated the possible involvement of these channels in the morphological development of the cells. Incubation of both the MAT-LyL...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051256
更新日期:1999-03-01 00:00:00
abstract::We report here on cholinergic neurons in the rat hippocampal formation that were identified by immunocytochemistry employing a monoclonal antibody against choline acetyltransferase (ChAT), the acetylcholine-synthesizing enzyme. In general, ChAT-immunoreactive cells were rare, but were observed in all layers of the hip...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215891
更新日期:1986-01-01 00:00:00
abstract::In the present study, we used ultrastructural cytochemistry to analyze the distribution of nuclear and cytoplasmic nucleic acids and polysaccharides, and electron spectroscopic imaging to map the element phosphorus in immature erythroid cells taken from two amphibians, the diploid Bufo ictericus and the tetraploid Odo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00305791
更新日期:1994-10-01 00:00:00
abstract::Intercellular bridges have been detected in ovarian follicle cells of Drosophila melanogaster. These bridges occur widely between follicle cells of previtellogenic chambers, while, in vitellogenic chambers, they become restricted to the columnar follicle cells. Usually, only one bridge is detectable between adjacent f...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224931
更新日期:1978-01-31 00:00:00
abstract::In model experiments with the use of horseradish peroxidase (HRP), two pathways of transport of substances to the adenohypophysis were studied, as well as the distribution of the tracer in the latter organ. The first pathway allows the tracer to penetrate from the intercellular milieu of the median eminence below the ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214248
更新日期:1984-01-01 00:00:00
abstract::With the use of immunocytochemistry, it was shown that both the supraoptic and paraventricular hypothalamic nuclei in humans contain at least two different neurophysins. These two human neurophysins are immunologically related to bovine neurophysin I and neurophysin II, respectively. One human neurophysin is associate...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00240096
更新日期:1979-02-15 00:00:00
abstract::Cells binding anti-bovine TASH beta serum were found exclusively in the rostral lobe of the adenohypophysis of the drake using the peroxidase-antiperoxidase complex unlabelled antibody method. The specificity of the binding of the anti-serum to TSH cells was established by relating the morphology and relative abundanc...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00234834
更新日期:1979-04-30 00:00:00