Abstract：
:We report here on cholinergic neurons in the rat hippocampal formation that were identified by immunocytochemistry employing a monoclonal antibody against choline acetyltransferase (ChAT), the acetylcholine-synthesizing enzyme. In general, ChAT-immunoreactive cells were rare, but were observed in all layers of the hippocampus proper and fascia dentata with a preponderance in zones adjacent to the hippocampal fissure and in the part of CA1 bordering the subiculum. All immunoreactive cells found were non-pyramidal neurons. They were relatively small with round or ovoid perikarya, which gave rise to thin spine-free dendrites. These hippocampal neurons were very similar to ChAT-immunoreactive cells in the neocortex of the same animals but were quite different from cholinergic neurons in the basal forebrain, medial septal nucleus, and neostriatum, which were larger and more intensely immunostained. Electron-microscopic analysis of ChAT-immunoreactive cells in the hippocampus and fascia dentata revealed synaptic contacts, mainly of the asymmetric type, on cell bodies and smooth proximal dendrites. The nuclei of the immunoreactive cells exhibited deep indentations, which are characteristic for non-pyramidal neurons. Our results provide evidence for an intrinsic source of the hippocampal cholinergic innervation in addition to the well-established septo-hippocampal cholinergic projection.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Frotscher M,Schlander M,Léránth Cdoi
10.1007/BF00215891subject
Has Abstractpub_date
1986-01-01 00:00:00pages
293-301issue
2eissn
0302-766Xissn
1432-0878journal_volume
246pub_type
杂志文章abstract：:This is the first detailed report about the collar enamel of the teeth of Polypterus senegalus. We have examined the fine structure of the collar enamel and enamel organ of Polypterus during amelogenesis by light and transmission electron microscopy. An immunohistochemical analysis with an antibody against bovine amel...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1305-4
更新日期：2012-02-01 00:00:00
abstract：:The endoneurial collagen sheath around teased nerve fibres following crush injury was studied by scanning electron microscopy and compared with uninjured sciatic nerve fibres and with fibres from the dystonic mutant mouse. Following crush injury the endoneurial collagen became more abundant than seen in untreated nerv...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210469
更新日期：1981-01-01 00:00:00
abstract：:We develop "autologous bone marrow cell infusion (ABMi) therapy" for the treatment of human decompensated liver cirrhosis and confirm the efficacy and safety of this treatment in multicenter clinical studies. With the goal of further expanding the applications of ABMi, we first cultured human bone marrow cells and the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1727-2
更新日期：2013-12-01 00:00:00
abstract：:MN9, a monoclonal antibody raised against mouse spermatozoa, specifically recognizes the equatorial segment of sperm head in several mammalian species, including humans. Colloidal gold-immuno-electron microscopy of mouse spermatozoa has shown that the antigen is localized in the space between the outer and inner acros...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00645047
更新日期：1992-12-01 00:00:00
abstract：:Nitric oxide and various neuropeptides in the myenteric plexus regulate esophageal motility. We sought colocalization of nitric oxide synthase and neuropeptides in frozen sections of mid-portion of smooth-muscled opossum esophagus using NADPH-diaphorase activity to mark the synthase and immunoreactivity to detect pept...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00331374
更新日期：1994-12-01 00:00:00
abstract：:We investigated the binding characteristics of two monoclonal antibodies, 4F3 and 3F8, which in the retina specifically stain Müller cells, both with protein blots and immunohistochemically in sections of various regions of the central nervous system of neonatal and adult cats and rats. Clear differences emerged betwe...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050887
更新日期：1997-09-01 00:00:00
abstract：:We have recently identified a tachykinin-related peptide (AmTRP) from the mushroom bodies (MBs) of the brain of the honeybee Apis mellifera L. by using direct matrix-assisted laser desorption/ionization with time-of-flight mass spectometry and have isolated its cDNA. Here, we have examined prepro-AmTRP gene expression...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0865-y
更新日期：2004-05-01 00:00:00
abstract：:In Rana temporaria tadpoles, fluorescent fibres appear in the prospective eminentia mediana, the pars intermedia and the pars distalis at Gosner's stage 25. During prometamorphosis the amount of fluorescent material increases around the developing primary capillary plexus in the eminentia mediana. In the pars intermed...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220236
更新日期：1976-09-01 00:00:00
abstract：:The etiologic basis of Parkinson's disease (PD), the second most common age-related neurodegenerative disorder, is unknown. Recent epidemiological and experimental studies indicate that exposure to environmental agents, including a number of agricultural chemicals, may contribute to the pathogenesis of this disorder. ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-004-0937-z
更新日期：2004-10-01 00:00:00
abstract：:Human fibroblasts and HeLa cells were treated with bunaftine (N-butyl-N-/2-(diethylamino)ethyl/-1-naphthalenecarboxamide ) in vitro. At concentrations of 0.5-2.0 mM, the drug caused contraction and rounding of the cells with loss of microvilli-like processes. Aggregates of dense, partly granular, partly fibrillar mate...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216519
更新日期：1984-01-01 00:00:00
abstract：:The attachment of the body of the snail Lymnaea stagnalis to the shell was studied by histochemistry and light and electron microscopy. Muscles of the body wall insert into the connective tissue by way of long thin projections of sarcolemma. The muscle cells end under the basement membrane of a specialised area of the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00224663
更新日期：1976-08-26 00:00:00
abstract：:Long-non-coding RNA (lncRNA) regulates gene expression through transcriptional and epigenetic regulation as well as alternative splicing in the nucleus. In addition, regulation is achieved at the levels of mRNA translation, storage and degradation in the cytoplasm. During recent years, several studies have described t...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-014-1885-x
更新日期：2014-06-01 00:00:00
abstract：:Carotid bodies of ducks show no detectable changes in ultrastructure up to periods of four weeks following the removal of 2-3 cm of the cervical vagus (decentralization). This indicates that the majority, and possibily all the nerves terminating on the glomus cells are afferent (sensory) in nature. These nerve endings...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218494
更新日期：1975-11-19 00:00:00
abstract：:The ultrastructure and distribution of adherens junctions in the intact adult lens of human, chicken, dove, rat, and rainbow trout were studied with thin-section electron microscopy, using an improved fixation containing a mixture of glutaraldehyde, lysine, and tannic acid. The nature of adherens junctions in the fibe...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220014
更新日期：1988-10-01 00:00:00
abstract：:Interstitial cells in the myenteric plexus and the deep muscular plexus of the small intestine of the c-kit mutant rats (Ws/Ws) and their normal siblings (+/+) were studied. c-Kit immunoreactivity was detected in two regions corresponding to the myenteric plexus and the deep muscular plexus in the jejunum of +/+ rats,...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051119
更新日期：1998-08-01 00:00:00
abstract：:The posterior rhombencephalic tela choroidea of the bullfrog was examined by electron microscopy. This membrane, the pia-ependymal roof of the caudal hindbrain, contains a large central region characterized by cuboidal ependymal cells which surround sizable microscopic apertures -- the interependymal pores. Ultrastruc...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220439
更新日期：1979-11-01 00:00:00
abstract：:In the compound eye of Notonecta glauca, the backswimmer, there is a small ventral region in which the rhabdoms differ in structure from those in the other parts of the eye. Here, among other unusual features, there is a special orientation of the microvilli of the central rhabdomeres, i.e., in most of the median eye ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222373
更新日期：1983-01-01 00:00:00
abstract：:Serial sections of human vaginal and keratinized oral-gingival epithelia were investigated for ciliary structures. Most melanocytes of the gingival epithelium lacked cilia, whereas almost all basal keratinocytes of the deeper portion of the epithelial ridges possessed one cilium each. In the suprabasal layers of the r...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215830
更新日期：1988-02-01 00:00:00
abstract：:Rhabdomeres of the Drosophila melanogaster canoemisl mutant ommatidia were twisted, branched, and often fused to each other. A considerable proportion of rhabdomeres were found to have fallen below the retinal basement membrane. Electron-microscopic observations of the mutant ommatidia revealed that microvilli, the su...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004419900107
更新日期：1999-12-01 00:00:00
abstract：:The elements Na, Cl and K are distributed heterogeneously in the larval cells of the four Malpighian tubules of Drosophila hydei. Elemental gradients are steeper when they are related to the dry weight in comparison to the weight of water. The high contents of Na, Cl and K in the cytoplasm close to the basal plasma me...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318555
更新日期：1993-06-01 00:00:00
abstract：:Considerable evidence indicates that the state of ocular connective tissues and their response in glaucomatous disease affect the degree of glaucoma damage. Both experimental and clinical data suggest that improved diagnostic and prognostic information can be derived from the assessment of the mechanical responsivenes...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-013-1603-0
更新日期：2013-08-01 00:00:00
abstract：:The postnatal maturation of regions of the epididymis and intragonadal segment of the deferens duct was studied in the rat by light- and transmission electron microscopy. Maturation of the genital duct starts in the distal cauda epididymidis and ductus deferens after one week of life, and one week later, in the more c...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215508
更新日期：1987-08-01 00:00:00
abstract：:The enteric nervous system appears to play a pivotal role in the functional recovery of the gastrointestinal tract after partial resection and reanastomosis, but the structural changes following surgery are not fully understood. The present study was designed to clarify the processes of myenteric plexus regeneration u...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327773
更新日期：1994-08-01 00:00:00
abstract：:Effects of steroids on the accumulation of glycoprotein gonadotropin (GTH) in pituitaries of juvenile trout were investigated by means of scanning cytophotometry applied to immunocytochemical preparations, and with the use of a radioimmunoassay. Effects on other aspects of GTH-cell activity were analyzed by measuring ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00226962
更新日期：1984-01-01 00:00:00
abstract：:Dexamethasone (Dex) regulates osteoblastic and adipocytic differentiation in mesenchymal progenitor cells through regulation of Wnt/β-catenin signaling. To elucidate the regulatory mechanisms underlying the effects of Dex, we examine the expression of Axin2, which is an intracellular inhibitor of Wnt/β-catenin signali...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1696-5
更新日期：2013-12-01 00:00:00
abstract：:The chemical nature of the thickened plasma membrane of cornified cells in stratified squamous epithelium was investigated in comparison with that in noncornified epithelium. Localizations of transglutaminase, molecular weight 92,000 daltons, and detection of epidermal cysteine proteinase inhibitor were effected with ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215516
更新日期：1987-08-01 00:00:00
abstract：:The objectives of these studies were to determine if (1) hypertrophy-stimulated myogenic regulatory factor (MRF) mRNA increases occur in the absence of proliferating satellite cells, and (2) acute hypertrophy occurs without satellite cell proliferation. Adult and aged quails were exposed to 0 or 2500 Rads gamma irradi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051314
更新日期：1999-06-01 00:00:00
abstract：:The papillary muscle of the cat heart's right ventricle has not been studied previously with quantitative ultrastructural techniques despite its wide use for functional studies. This tissue was perfusion-fixed, processed for electron microscopy, and morphometric techniques were used to assess the ultrastructural chara...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216200
更新日期：1983-01-01 00:00:00
abstract：:The electric organs of embryonic Torpedo marmorata have been reacted with three cationic stains to evaluate the appearance and distribution of anionic sites. Ruthenium red, alcian blue and lysozyme were used at different pHs and found to react in a time-related manner to anionic components within the interelectrocyte ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214662
更新日期：1987-10-01 00:00:00
abstract：:Nervous elements in the periodontal ligament and dental pulp of rat incisors were investigated by means of immunohistochemistry for neurofilament protein (NFP) and glia-specific S-100 protein. The periodontal ligament in the incisors was densely innervated by NFP-immunoreactive nerve fibers; the distribution of the ne...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215442
更新日期：1988-01-01 00:00:00
