Abstract：
:The chemical nature of the thickened plasma membrane of cornified cells in stratified squamous epithelium was investigated in comparison with that in noncornified epithelium. Localizations of transglutaminase, molecular weight 92,000 daltons, and detection of epidermal cysteine proteinase inhibitor were effected with a monoclonal antibody and a monospecific rabbit anti-inhibitor immunoglobulin, respectively, directed to the antigens. N-(7-dimethylamino-4-methylcoumarinyl)maleimide was used to demonstrate S-S cross-linking. In all keratinizing epithelia, the enzyme and inhibitor were deposited on membranes of granular cells. S-S bonds were formed in cornification with the appearance of electron-dense material by the inner leaflet. Both enzyme and inhibitors occurred on the corneal epithelium, but S-S linkage and the thickened plasma membrane did not form even at the last stage of maturation. On the other hand, the internal vaginal epithelium in the proestrous stage without keratinization contained the enzyme, but neither inhibitor nor S-S linkage. Both antigens and S-S bonds were detected when keratinization proceeded during estrus. The staining patterns in the epithelium near the vaginal introitus were identical to those in the skin. Cuboidal and simple epithelia exhibited none of those constituents. The findings indicated that heterogeneous components contribute to modification of the plasma membrane of cornified cells, but S-S cross-linkages are associated exclusively with formation of the ultrastructurally unique membrane structure. In addition, findings suggested hormonal regulation in the chemical modification of the membrane in estrogen-sensitive internal vaginal epithelium.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Nakano S,Fukuyama K,Epstein WLdoi
10.1007/BF00215516subject
Has Abstractpub_date
1987-08-01 00:00:00pages
331-6issue
2eissn
0302-766Xissn
1432-0878journal_volume
249pub_type
杂志文章abstract：:There is general consensus that enteroendocrine cells, EEC, containing the enteric hormone cholecystokinin (CCK) are confined to the small intestine and predominate in the duodenum and jejunum. Contrary to this, EEC that express the gene for CCK have been isolated from the large intestine of the mouse and there is evi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-017-2612-1
更新日期：2017-08-01 00:00:00
abstract：:Sipunculans, a small phylum of coelomated marine worms closely related to polychaete annelids, lack a true circulatory system. We have previously shown that the sipunculan Themiste petricola can form a cellular clot, without congealing, of cell-free coelomic fluid. The clot is formed by the aggregation of large granul...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-009-0912-9
更新日期：2010-03-01 00:00:00
abstract：:S-antigen-immunoreactive pinealocytes located in the deep portion of the pineal organ of inbred and wild pigmented mice give rise to long, beaded processes penetrating into the habenular and pretectal regions. In addition, the medial habenular nuclei and the pretectal area contain S-antigen-immunoreactive perikarya, w...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00313528
更新日期：1990-09-01 00:00:00
abstract：:Ontogenesis comprises a series of events including cell proliferation and apoptosis and resulting in the normal development of the embryo. Protein p53 has been described as being involved in the development of several animal species. The aim of this study was to analyze the expression of protein p53 during the morphog...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1720-9
更新日期：2014-01-01 00:00:00
abstract：:The colocalization of immunoreactivities to substance P and calcitonin gene-related peptide (CGRP) in nervous structures and their correlation with other peptidergic structures were studied in the stellate ganglion of the guinea pig by the application of double-labelling immunofluorescence. Three types of fibre were d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318563
更新日期：1993-06-01 00:00:00
abstract：:Gap junctions exist in the septa between the segments of the lateral giant axons in the ventral nerve cord of the crayfish Procambarus. A large increase in the resistance (uncoupling) of these gap junctions was brought about by mechanical injury to the axonal segments. Both thin sections and freeze-fracture preparatio...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217847
更新日期：1984-01-01 00:00:00
abstract：:To investigate the role of RhoA on the intracellular membrane dynamics of lysosomes in rat hepatoma cells (MM1), we analyzed the localization of lysosomal aspartic proteinase cathepsin D by confocal immunofluorescence microscopy in the dominant active RhoA-transfected cells. Here we show that the transfection of the d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000243
更新日期：2000-09-01 00:00:00
abstract：:The distribution of immunoreactive thyrotropin-releasing hormone (TRH) in the forebrain and hypophysis of Rana catesbeiana was studied by means of specific radioimmunoassay and immunohistochemistry based on peroxidase-antiperoxidase (PAP) techniques. A relatively high concentration of immunoassayable TRH is present in...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00212220
更新日期：1983-01-01 00:00:00
abstract：:Thyroid hormone (TH) plays an important role in regulating ovarian development. However, the mechanism involved remains unclear. Evidence suggests that glucose is essential for ovarian development, and its uptake is mediated by several glucose transporter proteins (Glut). We have investigated the effects of TH on Glut...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-016-2453-3
更新日期：2016-11-01 00:00:00
abstract：:Glycoproteins rich in mannosyl or glucosyl residues were analyzed in the subcommissural organ (SCO) and the pineal organ of the sheep (Ovis aries). By use of concanavalin A labelled with fluorescein isothiocyanate, fluorescent material was found both in ependymal and hypendymal cells of the SCO. In the pineal organ, e...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218562
更新日期：1986-01-01 00:00:00
abstract：:Estradiol injections increase serum level of calcium, amino acid, glucose, protein, ammonia and creatinine in immature Epinephelus akaara, and also increase levels of total lipid, cholesterol, phospholipid and esterified fatty acids. Hepatic protein, glycogen and lipid concentrations also rise after estradiol treatmen...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217235
更新日期：1984-01-01 00:00:00
abstract：:Transformation of the gastrula to the pluteus includes development of the ability of the larva to control the direction of ciliary beat and coordinate activities of the ciliary band with activities of the esophageal muscles (48-60 h, 15 degrees C). Glyoxylic acid-induced fluorescence shows several cells of the animal ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217887
更新日期：1983-01-01 00:00:00
abstract：:The development of the egg envelope and its incorporation into the larval cuticle of the polychaete Phragmatopoma lapidosa, was studied by correlative scanning and transmission electron microscopy. The mature egg possesses an envelope composed of five zones including an outer granular zone formed by the tips of the eg...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00225530
更新日期：1978-01-17 00:00:00
abstract：:To determine whether lipid-secreting cells have cytosolic Ca2+ concentration ([Ca2+]c)-related secretory mechanisms, morphological changes and intracellular calcium dynamics of Harderian glands of guinea pigs stimulated by secretagogue were studied by electron microscopy and Fura-2/AM digital image analysis. Control g...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327979
更新日期：1993-10-01 00:00:00
abstract：:Voltage-gated Na+ channels are expressed by highly metastatic MAT-LyLu cells, but not by poorly metastatic AT-2 cells, derived from the rodent Dunning model of prostatic cancer. We have investigated the possible involvement of these channels in the morphological development of the cells. Incubation of both the MAT-LyL...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051256
更新日期：1999-03-01 00:00:00
abstract：:Vitrification of biological specimens in liquid nitrogen can be achieved under high pressure (2,100 bars). This procedure obviates the use of aldehyde fixation and cryoprotection (glycerol). The present work demonstrates its applicability to the freeze-etching of mammalian brain tissue. Freeze-fracture repicas from ra...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00237626
更新日期：1980-01-01 00:00:00
abstract：:The so-called 'basement membrane' of arthropod compound eyes is known to be of heterogeneous origin (Odselius and Eloffson 1981). A major contribution in Diptera with open rhabdoms is provided by a pigmented component which lies at the basal end of the extracellular space of each ommatidium and fills it, the glial plu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222184
更新日期：1983-01-01 00:00:00
abstract：:The morphology of the antennal hair-sensilla of Periplaneta americana, their distribution and frequency on the antennal flagellum have been examined by transmission- and scanning-electron microscopy. The types of sensilla were distinguished with respect to physiologically relevant criteria such as wall structure and n...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00223221
更新日期：1978-07-13 00:00:00
abstract：:A recently developed immunohistochemical technique for serotonin (Steinbusch et al. 1978) was used in the present investigation to study the occurrence of this indoleamine in the granulated epithelial cells of neuro-epithelial bodies (NEB). Lungs from neonatal rabbits and pigs exhibit immunoreactive cell groups identi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217095
更新日期：1982-01-01 00:00:00
abstract：:By use of the immunofluorescence technique TSH-, LH- and ACTH-cells were localized in the hypophysis of tadpoles of Xenopus laevis. The first signs of the activity of these cells were observed in early stages of the development, i.e., stage 39 for ACTH, and stage 42 for TSH and LH. ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216218
更新日期：1982-01-01 00:00:00
abstract：:The ultrastructure of the contractile apparatus of the rat soleus muscle during the course of denervation atrophy was investigated. It was found that the ratio of thin the thick filaments increased in myofibrils of atrophying muscle fibers. Elevation of the ratio was observed as early as the second day after denervati...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216767
更新日期：1981-01-01 00:00:00
abstract：:Dysfunction of the retinal pigmented epithelium (RPE) is one of the first effects of dry age-related macular degeneration (AMD) with consequent blindness. Hence, patients affected by this retinal disorder could benefit from a cell-based transplantation strategy for RPE. Actually, an effective protocol to approach this...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-015-2185-9
更新日期：2015-10-01 00:00:00
abstract：:The processes involved in the regulation of bone cell metabolism are complex, including those implicated in bone cell coupling. This study was undertaken to develop a model that would permit real-time interaction between osteoclastic cells and osteoblasts in vitro. Osteogenic bone marrow stromal cells were isolated fr...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051160
更新日期：1998-10-01 00:00:00
abstract：:Effect of colchicine on the ultrastructure of taste bud cells was studied in the mouse. In untreated mice microtubules were abundant throughout the entire cytoplasm of type-III cells, but only in the apical cytoplasm of type-I cells. After 2 h of colchicine treatment, no microtubules were observed in any taste bud cel...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214555
更新日期：1985-01-01 00:00:00
abstract：:From its inception, tissue engineering has had three tenets: cells, biomaterial scaffolds and signaling molecules. Among the triad, cells are the center piece, because cells are the building blocks of tissues. For decades, cell therapies have focused on the procurement, manipulation and delivery of healthy cells for t...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-012-1339-2
更新日期：2012-03-01 00:00:00
abstract：:Back-labeling of regenerated electromotor neurons in the teleost Sternarchus albifrons was performed to test the hypothesis that, in regenerated spinal cord, incorrectly located electromotor neurons are eliminated because their axons do not reach the correct target area (electric organ). In each cross section examined...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217166
更新日期：1985-01-01 00:00:00
abstract：:In contrast to the vitellolysis zone which is involved in the degradation of the yolk, the cytoplasmic zone of the yolk syncytial layer, composed of many cytoplasmic organelles, is implicated in a secretory process. The granular endoplasmic reticulum of the latter is extremely well developed and organized into trabecu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236349
更新日期：1979-01-30 00:00:00
abstract：:To identify and describe ovarian follicles committed to undergo follicular degeneration (atresia), immature rats were primed with pregnant mare serum gonadotropin (PMSG). After PMSG treatment, preovulatory follicles develop but subsequently degenerate. Prior to the appearance of pyknotic nuclei (Stage I of atresia), d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233727
更新日期：1980-01-01 00:00:00
abstract：:Myosin and actin were localized by indirect immunofluorescence microscopy using specific antibodies prepared in rabbits against highly purified gizzard myosin and actin. A strong fluorescence staining with both antibodies was observed in rat corneal epithelial cells, anterior lens epithelial cells, rod inner segments,...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221771
更新日期：1977-07-19 00:00:00
abstract：:Somatostatin-like immunoreactivity was localized in nerve cell bodies and nerve terminals in the cat coeliac ganglion. Two types of somatostatin-immunoreactive cell bodies were revealed, the first being large (diameter 35 microns), numerous and weakly labelled, whereas the second was considerably smaller (diameter 10....
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327290
更新日期：1991-03-01 00:00:00
