Abstract:
:Previous studies have identified Dogiel type II neurons with cell bodies in the myenteric plexus of guinea-pig ileum to be intrinsic primary afferent neurons. These neurons also have distinctive electrophysiological characteristics (they are AH neurons) and 82-84% are immunoreactive for calbindin. They are the only calbindin-immunoreactive neurons in the plexus. Neurons with analogous shape and electrophysiology are found in submucosal ganglia, but, with antibodies used in previous studies, they lack calbindin immunoreactivity. An antiserum that is more effective in revealing calbindin in the guinea-pig enteric nervous system has been reported recently. In the present work, we found that this antiserum reveals the same population that was previously identified in myenteric ganglia, and does not reveal any further population of myenteric nerve cells. In submucosal ganglia, 9-10% of nerve cells were calbindin immunoreactive with this antiserum. The submucosal neurons with calbindin immunoreactivity were also immunoreactive for choline acetyltransferase, but not for neuropeptide Y (NPY) or vasoactive intestinal peptide (VIP). Small calbindin-immunoreactive neurons (average profile 130 microm2) were calretinin immunoreactive, whereas the large calbindin-immunoreactive neurons (average profile 330 microm2) had tachykinin (substance P) immunoreactivity. Calbindin immunoreactivity was seen in about 50% of the calretinin neurons and 40% of the tachykinin-immunoreactive submucosal neurons. It is concluded that, in the guinea-pig ileum, only one class of myenteric neuron, the AH/Dogiel type II neuron, is calbindin immunoreactive, but, in the submucosal ganglia, calbindin immunoreactivity occurs in cholinergic, calretinin-immunoreactive, secretomotor/vasodilator neurons and AH/Dogiel type II neurons.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Quinson N,Robbins HL,Clark MJ,Furness JBdoi
10.1007/s004410100395keywords:
subject
Has Abstractpub_date
2001-07-01 00:00:00pages
3-9issue
1eissn
0302-766Xissn
1432-0878journal_volume
305pub_type
杂志文章abstract::Expression of the non-muscle myosin heavy chain-B (NM-MHC-B, also denoted as the embryonic smooth muscle myosin heavy chain, SMemb) was examined in rat urinary bladder during growth in response to a partial urinary outflow obstruction. Following obstruction, the weight of the urinary bladder increased more than five-f...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410000262
更新日期:2001-05-01 00:00:00
abstract::The electric organs of embryonic Torpedo marmorata have been reacted with three cationic stains to evaluate the appearance and distribution of anionic sites. Ruthenium red, alcian blue and lysozyme were used at different pHs and found to react in a time-related manner to anionic components within the interelectrocyte ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214662
更新日期:1987-10-01 00:00:00
abstract::This study describes the morphology and distribution of setae on the lateral and medial flagella of the antennules of the spiny lobster Panulirus argus in an effort to identify antennular chemoreceptors in addition to the well-studied aesthetasc chemosensilla. Setae were examined using light and electron microscopy, a...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410100377
更新日期:2001-06-01 00:00:00
abstract::Call-Exner bodies are present in ovarian follicles of a range of species including human and rabbit, and in a range of human ovarian tumors. We have also found structures resembling Call-Exner bodies in bovine preantral and small antral follicles. Hematoxylin and eosin staining of single sections of bovine ovaries has...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051298
更新日期:1999-05-01 00:00:00
abstract::Electronmicroscopic and cytochemical studies were performed to localize interdigitating reticulum cells (IDC) in the popliteal lymph node of the rat. The morphological features of the IDC of the rat correspond to those described for other species, but also show similarities to normal macrophages in the rat. This is co...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220109
更新日期:1976-07-20 00:00:00
abstract::alpha-Smooth muscle (alpha SM) actin of endothermic vertebrates is selectively recognized by the monoclonal antibody anti-alpha SM-1. Immunoreactivity to this antibody has been shown to be localized in the NH2-terminal sequence Ac-EEED (Chaponnier et al. 1994). Among terrestrial ectothermic vertebrates, two amphibian ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00417867
更新日期:1995-09-01 00:00:00
abstract::The present communication describes an investigation of stimulation and inhibition of delta 5-3 beta-hydroxysteroid dehydrogenase in interrenal glands of tadpoles of Rana catesbeiana. Frozen sections of interrenal glands, together with kidneys, were prepared histochemically for assay of delta 5-3 beta-HSD activity. Co...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218950
更新日期:1987-12-01 00:00:00
abstract::Post-translational glycosylation of proteins with O-linked β-N-acetylglucosamine (O-GlcNAcylation) and changes of galectin expression profiles are essential in many cellular stress responses. We examine this regulation in the liver tissue of hibernating thirteen-lined ground squirrels (Ictidomys tridecemlineatus) repr...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-020-03185-x
更新日期:2020-07-01 00:00:00
abstract::The innervation of the anococcygeus muscle of the rat was investigated with regard to the histochemical features of nerve fibers within the muscle and to the location of the postganglionic autonomic neurons which are the source of these fibers. Acetylcholinesterase-positive fibers and catecholaminergic fibers are abun...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00571438
更新日期:1990-01-01 00:00:00
abstract::The surface ultrastructure of Kupffer cells in the rat liver has been studied by scanning electron microscopy (SEM). The results demonstrate that Kupffer cells are both significantly different and clearly distinct from endothelial cells. Kupffer cells have neither pores (and/or "sieve plates") nor fenestrations, all o...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00223015
更新日期:1975-12-10 00:00:00
abstract::Rainbow trout (Oncorhynchus mykiss) were exposed to ion-poor (soft) water to test the hypothesis that naturally induced proliferation of branchial chloride cells causes a thickening of the blood-to-water diffusion barrier. This was achieved by using a combination of scanning and transmission electron-microscopic techn...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050622
更新日期:1996-07-01 00:00:00
abstract::Nervous elements in the periodontal ligament and dental pulp of rat incisors were investigated by means of immunohistochemistry for neurofilament protein (NFP) and glia-specific S-100 protein. The periodontal ligament in the incisors was densely innervated by NFP-immunoreactive nerve fibers; the distribution of the ne...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215442
更新日期:1988-01-01 00:00:00
abstract::Neurotrophins NGF, BDNF, NT-3, and NT-4/5 are members of the neurotrophin family of proteins, which support the survival and induce differentiation of vertebrate neurons. We have studied the effects of neurotrophins on growth cones of embryonic sensory neurons. BDNF and NT-4/5 cause growth cone collapse and transient ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s004410050933
更新日期:1997-11-01 00:00:00
abstract::The structure of guppy (Poecilia reticulata) spermatogonia and spermatocytes has been studied using electron microscopy. The spermatogonia, situated at the apex of the seminiferous tubule, are almost all surrounded by a network of Sertoli cells; they have very diffuse chromatin and one or two large nucleoli. The cytop...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217139
更新日期:1984-01-01 00:00:00
abstract::Large conductance calcium-activated potassium channels (BK(ca)) are crucial for the regulation of cerebral vascular basal tone and might be involved in cerebral vasodilation relevant to migraine and stroke. We studied the differential gene expression of mRNA transcript levels and protein expression of the BK(Ca) chann...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-008-0701-x
更新日期:2008-12-01 00:00:00
abstract::The distribution and abundance of nitric oxide synthase (NOS)-containing neurons and their terminals in the gastrointestinal tract of the guinea-pig were examined in detail using NADPH diaphorase histochemistry and NOS immunohistochemistry. NOS-containing cell bodies were found in the myenteric plexus throughout the g...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00303090
更新日期:1994-07-01 00:00:00
abstract::Thy-1, a glycophosphatidylinositol-linked glycoprotein of the outer membrane leaflet, has been described in myofibroblasts of several organs. Previous studies have shown that, in fetal liver, Thy-1 is expressed in a subpopulation of ductular/progenitor cells. The aim of this study has been to investigate whether the l...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-007-0437-z
更新日期:2007-09-01 00:00:00
abstract::Pancreatic acinar cells of euthermic, hibernating and arousing individuals of the hazel dormouse Muscardinus avellanarius (Gliridae) have been observed at the electron-microscopic level and analysed by means of ultrastructural morphometry and immunocytochemistry in order to investigate possible fine structural changes...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051082
更新日期:1998-06-01 00:00:00
abstract::The chemical composition of intermediate filaments (IF's) in the ependyma of the subcommissural organ (SCO) of the Mongolian gerbil (Meriones unguiculatus) was investigated immunohistochemically in paraffin-embedded tissue. Antibodies against glial fibrillary acidic protein (GFAP), vimentin, neurofilament proteins and...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218796
更新日期:1989-03-01 00:00:00
abstract::This is the first report on the ultrastructural pattern of distribution of nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) in endothelial cells, using the rabbit aorta, and its colocalization with the neuronal isoform (type I) of nitric oxide synthase. About 30% of the endothelial cells showed a posit...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00314551
更新日期:1993-12-01 00:00:00
abstract::Dome epithelium (DE), the tissue covering lymphoid domes of gut-associated lymphoid tissues, was examined in both adult and neonatal rabbit appendix or sacculus rotundus to determine if dome epithelial cells matured earlier than epithelial cells covering adjacent villi. The localization of well-differentiated epitheli...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216473
更新日期:1987-06-01 00:00:00
abstract::Golgi- and fluorescence-histochemical studies in the chicken shown the presence of a sharply delimited group of aminergic neurons beneath the floor of the fourth ventricle at the mesen-metencephalic boundary. According to the observations reported in other avian species a homology can be established between the mammal...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216221
更新日期:1982-01-01 00:00:00
abstract::Pituitary adenylate cyclase-activating polypeptide (PACAP) and helospectin are two vasoactive intestinal polypeptide (VIP)-related neuropeptides that have recently been demonstrated in the mammalian gut; the aim of this study was to reveal their occurrence and localisation in the gastrointestinal tract, swimbladder, u...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00300227
更新日期:1994-09-01 00:00:00
abstract::The pinealocytes of the pig contain conspicuous dense bodies, the nature and role of which are not yet fully elucidated. The aim of this study was to demonstrate whether or not these structures are involved in the secretion process. The tannic acid-Ringer incubation (TARI)-method, which allows a clear-cut ultrastructu...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00313977
更新日期:1991-05-01 00:00:00
abstract::The accessory medulla with its associated pigment-dispersing hormone-immunoreactive neurons appears to be the pacemaker that controls the circadian locomotor activity rhythm of the cockroach Leucophaea maderae. To permit studies at the level of individual, identified, pacemaker neurons, we developed specific long-term...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051324
更新日期:1999-06-01 00:00:00
abstract::This investigation provides histochemical evidence for lysosomal storage of sulfated glycosaminoglycans (GAGs) in the interstitial cells of the renal cortex and in macrophage-like cells of the renal medullary zones of rats chronically treated with the drug tilorone. This compound is known to interfere with lysosomal d...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218958
更新日期:1987-12-01 00:00:00
abstract::The distribution of serotonin-immunoreactive cells in the lung of 4 species of reptiles was investigated. Serotonin-containing cells were found forming groups in the interconnecting septa in all 4 species studied, and also as solitary cells in Testudo graeca and Mauremys caspica. Serotonin-containing cells were also l...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216504
更新日期:1987-06-01 00:00:00
abstract::The notion that teeth contain stem cells is based on the well-known repairing ability of dentin after injury. Dental stem cells have been isolated according to their anatomical locations, colony-forming ability, expression of stem cell markers, and regeneration of pulp/dentin structures in vivo. These dental-derived s...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-007-0467-6
更新日期:2008-01-01 00:00:00
abstract::The termination of intrafusal muscle fibres was studied in muscle spindles of the tenuissimus muscle of the Chinese hamster. Most nuclear chain fibres terminated within the spindle capsule. The nuclear bag- and nuclear chain fibres that extended beyond the limit of the spindle capsule terminated in the endomysial conn...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218663
更新日期:1983-01-01 00:00:00
abstract::We conducted an immunoelectron-microscopic study for histamine (HA) in the enterochromaffin-like (ECL) cells of normal rats and rats given alpha-fluoromethylhistidine (alpha-FMH, 3 mg/kg per hour) via osmotic minipumps over a period of 24 h. The indirect immunoperoxidase procedure utilized a mouse monoclonal antibody ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410100448
更新日期:2001-11-01 00:00:00