Abstract:
:The present communication describes an investigation of stimulation and inhibition of delta 5-3 beta-hydroxysteroid dehydrogenase in interrenal glands of tadpoles of Rana catesbeiana. Frozen sections of interrenal glands, together with kidneys, were prepared histochemically for assay of delta 5-3 beta-HSD activity. Concentrations of 0.01, 0.1, 1, and 10 IU/ml of ACTH or of 0.01, 0.1, 1, and 10 micrograms/ml of cyanoketone were added to the incubation media. The reaction products of the histochemically prepared slides, in terms of absorbance, were scanned at a defined area with a computerized microscope spectrophotometer. The results indicate that ACTH causes a significant dose-response stimulation of delta 5-3 beta-HSD activity in tadpole interrenals; cyanoketone, on the other hand, causes significant dose-dependent inhibition.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Yu NW,Ku HH,Chang LT,Hsu CYdoi
10.1007/BF00218950subject
Has Abstractpub_date
1987-12-01 00:00:00pages
585-8issue
3eissn
0302-766Xissn
1432-0878journal_volume
250pub_type
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