Abstract：
:Human gingival fibroblasts were cultured with collagen fibrils. The precise process of collagen phagocytosis and the relationship between acid phosphatase activity and intracellular degradation of collagen were investigated by cytochemical methods at the ultrastructural level. The collagen fibrils were first engulfed at one end by cellular processes, or the cell membrane wrapped itself around the middle of the fibrils. Collagen phagocytosis induced acid phosphatase activity in the fibroblast Golgi-endoplasmic reticulum-lysosome system. By application of the tracer lanthanum, deposits were observed in the intercellular spaces and along the fibrils being phagocytosed. At this stage, primary lysosomes were seen in close proximity to the collagen being engulfed, but no signs of fusion were observed. When the fibrils had been interiorized in whole or in part, they ultimately became enclosed within phagosomes, and no tracer was observed along the interiorized portion of the fibrils. Primary lysosomes then fused with these collagen-containing phagosomes to form phagolysosomes. Collagen degradation occurred within these bodies even though the end of a fibril might have protruded outside of the cell. These results suggest that selective and controlled phagocytosis of collagen and intracellular digestion of it may play a central role in the physiological remodeling and metabolic breakdown of the collagen of connective tissues.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Yajima Tdoi
10.1007/BF00213929subject
Has Abstractpub_date
1986-01-01 00:00:00pages
253-60issue
2eissn
0302-766Xissn
1432-0878journal_volume
245pub_type
杂志文章abstract：:Previous investigations have demonstrated that peripheral insulin has a significant influence on brain function and that the interaction of insulin with neuropeptides in neuroendocrine regions may be pivotal for the regulation of body metabolism and energy balance. Since the various levels of interactions are only inc...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050833
更新日期：1997-06-01 00:00:00
abstract：:Brains and subesophageal ganglia from day 3.5 fifth stadium larvae of Manduca sexta were incubated in vitro with 4 nM tritiated ponasterone A, a 20-hydroxyecdysone analog, to determine whether uptake and specific binding of ecdysteroids occur at a cellular level. These tissues, which were taken just prior to the commi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318414
更新日期：1991-01-01 00:00:00
abstract：:Unique rod-shaped secretory granules were observed among oval or spherical secretory granules in GH cells of the anterior pituitary gland of musk shrew using the protein A-gold procedure combined with electron microscopy. The rod-shaped and spherical secretory granules were both immunoreactive by the immuno-gold metho...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215454
更新日期：1988-01-01 00:00:00
abstract：:By a regular immunoperoxidase method, alpha-1-proteinase inhibitor (Api) was demonstrated in pancreatic islets in individuals with and without genetic deficiency of Api. Subsequently a double immunoperoxidase method, with two different chromogens (diaminobenzidine-brown and 4-chloro-1-naphthol-blue), was applied on th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218078
更新日期：1986-01-01 00:00:00
abstract：:Interstitial cells in the myenteric plexus and the deep muscular plexus of the small intestine of the c-kit mutant rats (Ws/Ws) and their normal siblings (+/+) were studied. c-Kit immunoreactivity was detected in two regions corresponding to the myenteric plexus and the deep muscular plexus in the jejunum of +/+ rats,...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051119
更新日期：1998-08-01 00:00:00
abstract：:Haemophilic arthropathy is the major cause of disability in patients with haemophilia and, despite prophylaxis with coagulation factor concentrates, some patients still develop articular complications. We evaluate the feasibility of a tissue engineering approach to improve current clinical strategies for cartilage reg...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-016-2408-8
更新日期：2016-10-01 00:00:00
abstract：:We have previously shown that mice deficient in the gene for the myelin-associated glycoprotein (MAG) develop normal myelin in the peripheral nerves, but show axon and myelin degeneration at eight months of age, suggesting that MAG is involved in the maintenance of axon-Schwann cell integrity. The search for molecules...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050727
更新日期：1997-01-01 00:00:00
abstract：:A total of 20 Golgi-impregnated cone horizontal cells of Nannacara anomala (Cichlidae) were studied in alternating semi- and ultrathin sections in order to examine their connections with the overlying square mosaic of equal double and central single cones. Cone horizontal cells exhibiting three types of processes: (a)...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220825
更新日期：1976-11-24 00:00:00
abstract：:Histological, histochemical, and electron microscopical investigations of the rat pancreas, one to five hours after pilocarpine injection (4 mg in 1 ml normal saline solution), showed different secretory responses in periinsular and apoinsular acinar cells. The responsiveness to parasympathetic stimulation is markedly...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222119
更新日期：1975-08-01 00:00:00
abstract：:A study of the organisation of the locust dorsal ocellus shows that the structure is designed to provide the maximum possible effective aperture. The condenser-like cuticular lens and the dispersal of the rhabdome over a large proportion of the circumferential area of the retinula cells increases the light gathering p...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234742
更新日期：1979-02-28 00:00:00
abstract：:The expression of nicotinic acetylcholine receptors (nAChR) in fetal lung suggests maternal smoking during pregnancy effects newborn lung structure and function by the direct interaction of nicotine with nAChR in the developing lung. The recent identification of the lynx1 nAChR modulator protein in nicotinic neurons i...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-1077-9
更新日期：2005-05-01 00:00:00
abstract：:Morphogenesis of the arcuate nucleus of the rat from the 15th fetal day to the 6th postnatal day was investigated light and electron microscopically. The arcuate neurons exhibit a gradual development after the 15th fetal day. All cytoplasmic constituents are present in these nerve cells already during the last days of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236893
更新日期：1979-08-03 00:00:00
abstract：:The haemomonochorial placenta of the guinea-pig undergoes several quantitative changes between the 49th and 64th days of gestation, all of which are in such a direction as to increase the efficiency of transplacental transport. The fetal vessels become larger, the maternal vessels increase in surface area by prolifera...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220974
更新日期：1977-11-23 00:00:00
abstract：:The neurotensin-cell is identified immunohistochemically and ultrastructurally by differential counting of endocrine cells in the gut of a primate (Tupaia belangeri). Utilizing light microscopy, the EC-cells are identified by the Masson-Fontana silver stain; with the same method the neurotensin cells are not stained. ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220968
更新日期：1977-11-23 00:00:00
abstract：:The cutaneous glands of the forehead and the metatarsus were studied by histological and histochemical methods and electron microscopy in adult male and female impalas in various seasons of the year. All glandular areas consist of apocrine and holocrine glands, which, however, occur in different proportions. Our findi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051068
更新日期：1998-05-01 00:00:00
abstract：:For the study of the hypothalamo-hypophysial system of Xenopus laevis tadpoles, hypothalamic lesions were made by means of the electrocoagulation technique. Lesioning of the ventral region of the preoptic nucleus resulted in a decrease of the number of ACTH cells in the pars distalis of the pituitary gland and in a di...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219304
更新日期：1976-06-11 00:00:00
abstract：:Noses are extremely sophisticated chemical detectors allowing animals to use scents to interpret and navigate their environments. Odor detection starts with the activation of odorant receptors (ORs), expressed in mature olfactory sensory neurons (OSNs) populating the olfactory mucosa. Different odorants, or different ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-020-03327-1
更新日期：2021-01-06 00:00:00
abstract：:Adenosine 5'-triphosphate is known to function as a potent extracellular messenger, producing its effects via a distinct family of cell surface receptors. Different receptor subtypes have been shown to modulate different cellular functions such as proliferation, differentiation and apoptosis. We have investigated the ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-1149-x
更新日期：2005-09-01 00:00:00
abstract：:Although several studies have shown a dysregulation of microRNA (miRNA) expression profiles in cutaneous melanoma, there has been little research on the miRNA machinery itself. In this study, we investigated the mRNA expression profiles of different miRNA machinery components in primary cutaneous malignant melanoma (P...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1446-0
更新日期：2012-10-01 00:00:00
abstract：:Cytochemical methods for the demonstration of p-nitrophenylphosphatase (p-NPPase) at the electron-microscopic level were applied to avian osteoclasts to elucidate some of the functional differences among ruffled border, other membrane systems, and cellular organelles. The localization of p-NPPase activity occurred in ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218381
更新日期：1986-01-01 00:00:00
abstract：:Osteoarthritis (OA) is the most common joint disease with an unsatisfactory therapy outcome and characterized by the degradation of articular cartilage and synovial inflammation. Here, we isolated bone marrow mesenchymal stem cells (BMSCs) from rat's bone marrow and BMSC-derived exosome (BMSCs-Exo) from BMSCs successf...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03319-1
更新日期：2021-01-06 00:00:00
abstract：:The distribution of the glycoprotein, mucin 1 (MUC1), was determined in lactating guinea-pig mammary tissue at the resolution of the electron microscope. MUC1 was detected on the apical plasma membrane of secretory epithelial cells, the surface of secreted milk-fat globules, the limiting membranes of secretory vesicle...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100351
更新日期：2001-04-01 00:00:00
abstract：:Neuromuscular terminals of a single motoneuron to four muscles (CPV7a, GM5a, CV2, and CV3) in the stomach of the blue crab Callinectes sapidus showed structural evidence for the exocytotic release of dense-core vesicles exclusively at synapses. The primary evidence was the appearance of dense cores in the synaptic cle...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050897
更新日期：1997-09-01 00:00:00
abstract：:It is possible to determine statistically the repartitions of shapes or sizes in a population of particles from the recurrence of shapes or sizes of their outlines in microscopical sections. This method was applied to a population of 1326 follicular profiles randomly sampled from 30 ovaries of pubescent homozygous "Nu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219416
更新日期：1976-07-30 00:00:00
abstract：:In developing bioassays for estrogenic effects, vitellogenin (Vtg) induction and zona radiata protein (Zr-protein) induction in males and juveniles of oviparous vertebrates have been used as sensitive biomarkers for estrogenicity. Nonylphenol (NP) produces similar and parallel expression patterns of Vtg and Zr-protein...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0543-y
更新日期：2008-03-01 00:00:00
abstract：:The octavo-lateral efferent system of several anuran species was studied by means of retrograde transport of horseradish peroxidase. This system is organized similarly in all larval anurans and in all adult aglossids. All have two groups of efferent neurons in the nucleus reticularis medialis between the VIIIth and th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214812
更新日期：1982-01-01 00:00:00
abstract：:Scanning electron microscopy was used to investigate the morphological changes of the tail musculature of the metamorphosing anuran tadpole, attention being focused on phagocytosis by macrophages. Muscle fibers were stained en bloc with silver and freeze-fractured during dehydration, or torn after drying. Samples were...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214574
更新日期：1985-01-01 00:00:00
abstract：:Appearance of immunoreactive corticotropin-releasing factor (CRF)-containing neurons was studied in developing hypothalamus of the rat by use of antisera against rat- and ovine CRF. These neurons were first recognized in the lateral and paraventricular nuclei on days 15.5 and 16.5 of gestation, respectively, when anti...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219870
更新日期：1984-01-01 00:00:00
abstract：:Neurons displaying FMRFamide(Phe - Met - Arg - Phe - NH2)-like immunoreactivity have recently been implicated in neural plasticity in salmon. We now extend these findings by describing the extent of the FMRF-like immunoreactive (FMRF-IR) system in the brain, retina and olfactory system of sockeye salmon parr using the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00313518
更新日期：1990-09-01 00:00:00
abstract：:Generally, the CRF-like activity of vasopressin is studied in experiments involving adrenalectomy and corticosteroid replacement. In order to avoid this complex type of stress, male and female (diestrus, estrus) rats were exposed to 5 min to immobilization stress and sacrificed 5, 15, and 30 min thereafter. After a su...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209276
更新日期：1978-05-29 00:00:00
