Abstract:
:Thy-1, a glycophosphatidylinositol-linked glycoprotein of the outer membrane leaflet, has been described in myofibroblasts of several organs. Previous studies have shown that, in fetal liver, Thy-1 is expressed in a subpopulation of ductular/progenitor cells. The aim of this study has been to investigate whether the liver myofibroblasts belong to the Thy-1-positive subpopulation of the adult liver. The expression of Thy-1 has been studied in normal rat liver, in the rat liver regeneration model following 2-acetylaminofluorene treatment and partial hepatectomy (AAF/PH), and in isolated rat liver cells, at the mRNA and protein levels. In normal rat liver, Thy-1 is detected in sparse cells of the periportal area, whereas 7 days after PH in the AAF/PH model, a marked increase of the number of Thy-1-positive cells is detectable by immunohistochemistry. Comparative immunohistochemical analysis has revealed the co-localization of Thy-1 and smooth muscle actin, but not of Thy-1 and cytokeratin-19, both in normal rat liver and in the AAF/PH model. Investigation of isolated rat liver cell populations has confirmed that liver myofibroblasts are Thy-1-positive cells, whereas hepatocytes, hepatic stellate cells, and liver macrophages are not. Thy-1 is the first cell surface marker for identifying liver myofibroblasts in vivo and in vitro.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Dudas J,Mansuroglu T,Batusic D,Saile B,Ramadori Gdoi
10.1007/s00441-007-0437-zsubject
Has Abstractpub_date
2007-09-01 00:00:00pages
503-14issue
3eissn
0302-766Xissn
1432-0878journal_volume
329pub_type
杂志文章abstract::Survival of mesenchymal stem cells (MSCs) against oxidative stress and inflammation is vital for effective stem cell therapy. The reactive oxygen species (ROS) result in apoptosis and release of inflammatory mediators. Adipose-derived stem cells (ASCs) have shown promise for stem cell therapy owing to their anti-infla...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-018-2857-3
更新日期:2018-10-01 00:00:00
abstract::Dorsal root ganglia of chick embryos were cultured for one to four weeks on Maximow-slides. Puromycin was added to cultures for a pulse of 30' in a dose of 100 mug/ml medium. Particular interest was given to the ultrastructural features of the glia-neuron relations. Puromycin caused a shrinkage of the glial processes ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00225762
更新日期:1975-07-23 00:00:00
abstract::The membranes of the microvilli of UV- and green-photoreceptors of the ant Myrmecia gulosa have been studied with the freeze-fracture technique. Both inner fracture faces, the cytoplasmic P-face and the extracellular E-face, are covered by globular particles. The P-face particles appear to be randomly distributed, occ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218715
更新日期:1976-12-10 00:00:00
abstract::The distribution of immunoreactive thyrotropin-releasing hormone (TRH) in the forebrain and hypophysis of Rana catesbeiana was studied by means of specific radioimmunoassay and immunohistochemistry based on peroxidase-antiperoxidase (PAP) techniques. A relatively high concentration of immunoassayable TRH is present in...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00212220
更新日期:1983-01-01 00:00:00
abstract::Filamentous intranuclear rodlets were found in a majority of cells in a bronchiolar neuroepithelial body of a rabbit. These rodlets appear to be similar to structures that have been described primarily in neural tissues. They are composed of large bundles of microfibrils made up of smaller subbundles. No close associa...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00210521
更新日期:1981-01-01 00:00:00
abstract::Experimental autoimmune orchitis (EAO) in the rat is the primary chronic animal model for the investigation of one of the main causes of male infertility, viz., testicular inflammation. Dendritic cells (DC) are potent antigen-presenting cells that play a fundamental role in autoimmune disease. We investigated the numb...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-0129-5
更新日期:2006-05-01 00:00:00
abstract::To examine the selective permeability of the nephrons of lower vertebrates, the permeability of the glomerulus in the kidney of an arctic lamprey, Entosphenus japonicus (Martens), to native anionic ferritin or cationized ferritin was studied by observing the distribution of ionized anionic groups in renal tissues. The...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00226944
更新日期:1984-01-01 00:00:00
abstract::VIP-containing neurons were localized in lungs from dogs, cat, and human subjects by means of the indirect immunofluorescence technique. Nerve fibers and terminals were observed in the smooth muscle layer and glands of airways, and within the walls of pulmonary and bronchial vessels, especially at the medial-adventiti...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00210505
更新日期:1981-01-01 00:00:00
abstract::Nerve fibers connecting the brain with the pineal gland of the Mongolian gerbil (central pinealopetal fibers) were investigated by means of light and electron microscopy. Several myelinated fibers penetrate from the brain into the deep pineal gland, extend further into the pineal stalk and continue to the superficial ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00213804
更新日期:1983-01-01 00:00:00
abstract::The excretory system of Macrobiotus richtersi consists of one dorsal and two lateral components and shows a high degree of structural complexity. In each of these a tricellular external lobe and a column can be distinguished, the two parts being connected distally. The surface of the lobe cells is increased by deep ba...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00239339
更新日期:1980-01-01 00:00:00
abstract::Young rats were injected with 3H-fucose and killed at 10 min, 1, 4 and 24 h thereafter. Samples of the pancreas were processed for light- and electron-microscopic radioautography and then analyzed quantitatively. Fucose label was taken up in the supranuclear region of acinar pancreatic cells that were maximally labele...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222243
更新日期:1983-01-01 00:00:00
abstract::The treatment of isolated protoplasmic strands of Physarum polycephalum with 2.5% ethanol in a physiological salt solution under isometric conditions induces the formation of a large amount of mostly longitudinally organized actomyosin fibrils in the endoplasmic channel, a region normally free of actomyosin fibrils. T...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218017
更新日期:1985-01-01 00:00:00
abstract::During the reproductive period of the male stickleback, structural and functional changes of the kidney take place, both in the glomeruli and in the renal tubule cells. The structural changes in the glomeruli involve almost all glomerular components and point to a reduction of the glomerular filtration rate. The purpo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00210146
更新日期:1981-01-01 00:00:00
abstract::Adrenal medullary chromaffin cells are derivatives of the neural crest and are widely believed to share a common sympathoadrenal (SA) progenitor with sympathetic neurons. For decades, the adrenal cortical environment was assumed to be essential for channelling SA progenitors towards an endocrine chromaffin cell fate. ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0213-5
更新日期:2006-09-01 00:00:00
abstract::Sacciform cells containing an acidophilic, proteinaceous secretion, were identified in the epidermis of the brown trout and Arctic char. This cell type increased in number during the chronic stages of infestation by the ectoparasitic flagellate, Ichthyobodo sp., in immature brown trout, and decreased during sexual mat...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218307
更新日期:1987-02-01 00:00:00
abstract::The pancreatic endocrine tissue of Fugu rubripes rubripes consists of numerous round principal islets (Brockmann bodies) of various sizes scattered around the gall-bladder. The endocrine cells are divided into A-, B-, D-, and Ff-cells. Each cell type was identified by comparing thick and thin sections in both light an...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222157
更新日期:1976-10-29 00:00:00
abstract::The distribution of MAP2 and actin in dendritic spines of the visual and cerebellar cortices, dentate fascia, and hippocampus was determined by using immunogold electron microscopy. By this approach, we have confirmed the presence of MAP2 in dendritic spines and identified substructures within the spine compartment sh...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00225592
更新日期:1989-06-01 00:00:00
abstract::The membrane systems of cardiac muscle cells of the euphausiacean Meganychtiphanes norvegica are described. Transverse tubules are found both at the Z-band level (TZ-tubules) and at the H-band level (Th-tubules). Within the sarcomere narrow longitudinal tubules branch off from the TZ-tubules. At the H-band level these...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00223109
更新日期:1977-07-15 00:00:00
abstract::(1) The basiconic sensilla on the antennae of Calliphora resemble other insect epidermal sensilla; one or several bipolar sense cells are surrounded by three non-neural cells. (2) The apical cell membrane of the tormogen cell (one of the three accessory cells) forms microvilli coated internally with particles. (3) In ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00209140
更新日期:1978-06-08 00:00:00
abstract::In the central nervous system of the male domestic flow, metenkephalin (ENK) immunoreactive perikarya and fiber tracts as well as extensive but sharply delimited fiber networks were visualized by means of the PAP technique. The most striking results were: (1) The demonstration of an association of ENK-containing struc...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00236920
更新日期:1980-01-01 00:00:00
abstract::Homeostatic cell turnover has been extensively characterized in mammals. In their adult tissues, lost or aging differentiated cells are replenished by a self-renewing cohort of stem cells. The stem cells have been particularly well studied in the intestine and are clearly identified by the expression of marker genes i...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-017-2692-y
更新日期:2017-12-01 00:00:00
abstract::Pax genes are expressed in specific patterns in the nervous system during development and in the adult. Recent findings suggest a link between the expression of Pax-2 and axonal guidance. Mice with a targeted deletion of Pax-2 are an excellent tool for studying axonal pathfinding at the molecular level, especially wit...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s004410050923
更新日期:1997-11-01 00:00:00
abstract::The intrapancreatic localization and the effects on basal and stimulated insulin secretion of neuropeptide Y (NPY) were investigated in the mouse. Immunocytochemistry showed NPY to be confined to intrapancreatic nerve fibers mainly associated with blood vessels. Fine varicose NPY fibers were also detected in the exocr...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF01239960
更新日期:1987-04-01 00:00:00
abstract::An ectodermal network of nerve cells has been observed in the echinopluteus. It seems to connect the ectodermal cell bodies with each other and with deeper parts of the nerve system. Together with the neurohumour containing parts of the ventral and oral ciliated bands, the nerve cells probably provide the larva with t...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219793
更新日期:1977-04-07 00:00:00
abstract::The distribution of the glycoprotein, mucin 1 (MUC1), was determined in lactating guinea-pig mammary tissue at the resolution of the electron microscope. MUC1 was detected on the apical plasma membrane of secretory epithelial cells, the surface of secreted milk-fat globules, the limiting membranes of secretory vesicle...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410100351
更新日期:2001-04-01 00:00:00
abstract::Affinity of lymphoid cells for the microenvironment of germinal centers (GC), as detectable in transfer experiments by rapid homing in spleen GC from the blood, is a capacity expressed by only a subset of lymphoid cells, in particular by those constituting a GC. However, when introduced into the blood stream, these ce...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215160
更新日期:1984-01-01 00:00:00
abstract::A total of 20 Golgi-impregnated cone horizontal cells of Nannacara anomala (Cichlidae) were studied in alternating semi- and ultrathin sections in order to examine their connections with the overlying square mosaic of equal double and central single cones. Cone horizontal cells exhibiting three types of processes: (a)...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220825
更新日期:1976-11-24 00:00:00
abstract::In Nassarius reticulatus the nuclei and nucleoli undergo important morphological changes from the zygote to the 16-cell stage. In the zygote and in the trefoil (2-cell) and 4-cell stage, several agranular, fibrillar nucleolus-like bodies 1 mum diameter are present in the interphase nucleus. Granular nucleoli first app...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215313
更新日期:1976-05-13 00:00:00
abstract::The pineal gland structure and ultrastructure in the Northern (Blarina brevicauda) and Southern short-tailed shrew (Blarina carolinensis) are described by light and electron microscopy. Results observed were similar to other mammals of Insectivora described previously, specifically, the hedgehog (Erinaceus europaeus) ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-018-2897-8
更新日期:2018-12-01 00:00:00
abstract::In the current experiment, the combined regime of resveratrol and a Wnt-3a inhibitor, sulindac, were examined on the angiogenic potential of cancer stem cells from human colon adenocarcinoma cell line HT-29 during 7 days. Cancer stem cells were enriched via a magnetic-activated cell sorter technique and cultured in en...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-019-02998-9
更新日期:2019-06-01 00:00:00