Abstract:
:This is the first report on the ultrastructural pattern of distribution of nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) in endothelial cells, using the rabbit aorta, and its colocalization with the neuronal isoform (type I) of nitric oxide synthase. About 30% of the endothelial cells showed a positive reaction for NADPH-d compared to about 6% for nitric oxide synthase immunoreactivity. Simultaneous double histochemical-immunocytochemical labelling procedures indicate that all of the cells displaying nitric oxide synthase-positive reactivity also contained NADPH-d; the remainder of NADPH-d-positive endothelial cells were negative for this isoform of nitric oxide synthase. Nitric oxide synthase-immunogold labelling was mostly associated with free ribosomes, while NADPH-d activity was distributed largely in patches in the cytoplasm and in association with the cell membrane.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Loesch A,Belai A,Burnstock Gdoi
10.1007/BF00314551subject
Has Abstractpub_date
1993-12-01 00:00:00pages
539-45issue
3eissn
0302-766Xissn
1432-0878journal_volume
274pub_type
杂志文章abstract::The association between adipose tissue and immunity has been established and fat-associated lymphoid clusters (FALCs) are considered as a source of immune cells. We discovered lymphoid clusters (LCs) in mouse mediastinal fat tissues (MFTs). In Th1-biased C57BL/6N (B6), Th2-biased DBA/2Cr (DBA) and autoimmune-prone MRL...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-014-1889-6
更新日期:2014-09-01 00:00:00
abstract::Black tiger shrimps (Penaeus monodon) are able to survive and can be reared under various salinities, possibly by the cellular adaptation of their excretory system, particularly the antennal gland, which is known to regulate body fluid in crustaceans. We have investigated the morphological and biochemical alterations ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-010-0971-y
更新日期:2010-06-01 00:00:00
abstract::The ultrastructural changes induced by the topical application of retinol acetate on hamster cheek pouch epithelium were evaluated using stereological analysis. Electron micrographs were prepared of the basal and superficial regions of the nucleated cell layer of the epithelium obtained from 3 treated and 3 control an...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214783
更新日期:1982-01-01 00:00:00
abstract::Olfactory marker protein (OMP) was first described as a protein expressed in olfactory receptor neurons (ORNs) in the nasal cavity. In particular, OMP, a small cytoplasmic protein, marks mature ORNs and is also expressed in the neurons of other nasal chemosensory systems: the vomeronasal organ, the septal organ of Mas...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-020-03349-9
更新日期:2021-01-15 00:00:00
abstract::Research in mammals has demonstrated a variety of regulatory effects of vasopressin and oxytocin on endocrine functions of the anterior pituitary gland. Less evidence is available regarding the hypophysiotropic action of arginine vasotocin (AVT) comprising vasopressic and oxytocic activities in birds. Some hypophysiot...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-008-0636-2
更新日期:2008-08-01 00:00:00
abstract::The three-dimensional ultrastructure of the pineal body of the rat is described on the basis of freeze-fractured preparations. The pineal capsule consists of irregular cells with very flat and perforated processes. Through these openings, extremely branched canaliculi, extending to almost every pineal cell, communicat...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00238052
更新日期:1979-09-02 00:00:00
abstract::Human gingival fibroblasts were cultured with collagen fibrils. The precise process of collagen phagocytosis and the relationship between acid phosphatase activity and intracellular degradation of collagen were investigated by cytochemical methods at the ultrastructural level. The collagen fibrils were first engulfed ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00213929
更新日期:1986-01-01 00:00:00
abstract::At the sites where a vein penetrates through the dura mater, two aspects deserve particular attention: (i) The delineation of the perivascular cleft, a space belonging to the interstitial cerebrospinal fluid (CSF) compartment, toward the interior hemal milieu of the dura mater. (ii) The relationship between the periva...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214011
更新日期:1988-03-01 00:00:00
abstract::To gain a better understanding of the neuroplasticity of sympathetic neurons during postnatal ontogenesis, the distribution of neuronal nitric oxide synthase (nNOS) immunoreactivity was studied in sympathetic preganglionic neurons (SPN) in the spinal cord (Th2 segment) of female Wistar rats at different ages (newborn,...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-018-2925-8
更新日期:2019-02-01 00:00:00
abstract::The present electron microscopic autoradiographic study includes a quantitative analysis of osteoclasts in vitro using tritiated leucin as a protein tracer. A significant increase in the grain density over the ruffled border and the underlying resportion zone was demonstrated two hours post pulse whereas the grain den...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219726
更新日期:1976-04-28 00:00:00
abstract::Fibroblast growth factors (Fgfs) are proteins with diverse functions in development, repair, and metabolism. The human Fgf gene family with 22 members can be classified into three groups, canonical, intracellular, and hormone-like Fgf genes. In contrast to canonical and intracellular Fgfs identified in invertebrates a...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-010-1024-2
更新日期:2010-10-01 00:00:00
abstract::The medial preoptic nucleus of the Japanese quail is a testosterone-sensitive structure that is involved in the control of male copulatory behavior. The full understanding of the role played by this nucleus in the control of reproduction requires the identification of its afferent and efferent connections. In order to...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00343944
更新日期:1994-06-01 00:00:00
abstract::Pituitary somatotrops were localized in four species from the genus Oncorhynchus using an antiserum to chum salmon (Oncorhynchus keta) growth hormone. The antiserum cross-reacted specifically with the acidophils in the caudal pars distalis of all species tested. Corroborative immunodiffusion studies were also conducte...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218126
更新日期:1983-01-01 00:00:00
abstract::We have examined IgG Fc receptor (FcR) activity of human and rabbit arachnoid granulations and leptomeninges using antibody (IgG)-coated erythrocytes (EIgG), covalently crosslinked IgG dimers, trimers and oligomers, immune complexes, aggregated Fc fragments and a monoclonal anti-human neutrophil Fc receptor antibody, ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216489
更新日期:1987-06-01 00:00:00
abstract::Boar spermadhesin (AWN) is a 14-kDa multifunctional protein, attached to the surface of the spermatozoa and involved in sperm capacitation and zona pellucida binding. The cellular origin of AWN was previously unknown. Moreover, the region of the male genital tract in which AWN becomes attached to the surface of sperma...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00319144
更新日期:1995-10-01 00:00:00
abstract::Prolactin cells were identified by means of immunocytochemistry with protein-A gold as a marker on ultrathin sections of the pituitary gland of young (3-4 months), middle-aged (16-19 months), and aged (26-30 months) male Wistar rats. Point-counting volumetry revealed that the prolactin (PRL) cell-volume density in mid...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215844
更新日期:1988-02-01 00:00:00
abstract::Their strategic location between blood and tissue and their constitutive properties allow endothelial cells (EC) to monitor the transport of plasma molecules, by employing bidirectional receptor-mediated and receptor-independent transcytosis and endocytosis, and to regulate vascular tone, cellular cholesterol and lipi...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-008-0678-5
更新日期:2009-01-01 00:00:00
abstract::Complex carbohydrates in secretory granules and at the apical cell surface of mouse gastric mucoid cells were studied during embryogenesis and in the early postnatal period by various cytochemical methods; the periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) and tannic acid-uranyl acetate (TA-UA) procedu...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214559
更新日期:1985-01-01 00:00:00
abstract::Successful embryo implantation depends on intricate epithelial-stromal cross-talk. However, molecular modulators involved in this cellular communication remain poorly elucidated. Using multiple approaches, we have investigated the spatiotemporal expression and regulation of serine protease inhibitor Kazal type 3 (SPIN...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-010-1013-5
更新日期:2010-09-01 00:00:00
abstract::The gene encoding H1t, a testicular variant of histone H1, is expressed in mammals during spermatogenesis. Northern blot and in situ hybridization has detected H1t mRNA only at the stage of pachytene spermatocytes. We have extended this analysis to more sensitive approaches and demonstrate, by RNase protection and ele...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050986
更新日期:1998-01-01 00:00:00
abstract::Corneal fibroblasts, major cellular components of the corneal stroma, are loosely arrayed between collagen lamellae. They play an important role in the metabolic and physiological homeostasis mechanisms by which the cornea is kept transparent. This paper deals with the demonstration of the gap junctions between the co...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215533
更新日期:1987-08-01 00:00:00
abstract::Dorsal root ganglia of chick embryos were cultured for one to four weeks on Maximow-slides. Puromycin was added to cultures for a pulse of 30' in a dose of 100 mug/ml medium. Particular interest was given to the ultrastructural features of the glia-neuron relations. Puromycin caused a shrinkage of the glial processes ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00225762
更新日期:1975-07-23 00:00:00
abstract::The pituitary gland of the flounder, Pleuronectes flesus, showed several unusual cytological features. Between the RPD and the PPD was a zone of cells that stained purple with Alcian blue--PAS--orange G. Many of these cells were apparently degenerating. In the PPD the strands and coils of presumptive STH cells showed ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00225528
更新日期:1975-01-01 00:00:00
abstract::To investigate the control of the timing in the epithelio-mesenchymal transformation of the neural crest into a migrating population, neural anlagen (neural tube plus crest) were isolated from 2-day quail embryos by proteases in the presence of CA++ and explanted onto substrates favourable for neural crest cell migrat...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218011
更新日期:1985-01-01 00:00:00
abstract::Cortisone-treated Buffalo rats have been parabiosed with untreated controls of the same age. The optical and electron microscopy, including histochemistry, of costal cartilage of these rats has been compared with that in single cortisone treated rats, single controls, and control parabiosed with control rats, at 14 an...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00223122
更新日期:1978-05-18 00:00:00
abstract::The chemical composition of intermediate filaments (IF's) in the ependyma of the subcommissural organ (SCO) of the Mongolian gerbil (Meriones unguiculatus) was investigated immunohistochemically in paraffin-embedded tissue. Antibodies against glial fibrillary acidic protein (GFAP), vimentin, neurofilament proteins and...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218796
更新日期:1989-03-01 00:00:00
abstract::Progenitor stem cells have been identified, isolated and characterized in numerous tissues and organs. However, their therapeutic potential and the use of these stem cells remain elusive except for a few progenitor cells from bone marrow, umbilical cord blood, eyes and dental pulp. The use of bone marrow-derived hemat...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-012-1471-z
更新日期:2012-10-01 00:00:00
abstract::Nitric oxide and various neuropeptides in the myenteric plexus regulate esophageal motility. We sought colocalization of nitric oxide synthase and neuropeptides in frozen sections of mid-portion of smooth-muscled opossum esophagus using NADPH-diaphorase activity to mark the synthase and immunoreactivity to detect pept...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00331374
更新日期:1994-12-01 00:00:00
abstract::Calcitonin gene-related peptide-immunoreactive cells were identified within the epithelium of distal conducting airways in the human fetus and infant. Several peptides and amines, including calcitonin, have been identified previously within a specific population of airway epithelial cells. These cells, referred to as ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218949
更新日期:1987-12-01 00:00:00
abstract::A tissue-engineered heart valve can be an alternative to a prosthetic valve in heart valve replacement; however, it is not fully efficient in terms of long-lasting functionality, as leaflets in engineered valves do not possess the trilayered native leaflet structure. Previously, we developed a flat, trilayered, orient...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-020-03241-6
更新日期:2020-11-01 00:00:00