Abstract：
:To investigate the control of the timing in the epithelio-mesenchymal transformation of the neural crest into a migrating population, neural anlagen (neural tube plus crest) were isolated from 2-day quail embryos by proteases in the presence of CA++ and explanted onto substrates favourable for neural crest cell migration. Explants isolated before normal migration had commenced required 3-8 h in vitro before neural crest cells started migration, but explants obtained at migratory stages showed an immediate onset of migration. The schedule was similar to that expected in vivo. When pre-migratory neural anlagen were isolated by protease in Ca++-and Mg++-free (CMF) medium, or when the protease was followed by a brief (5 min) exposure to CMF medium, neural crest cell migration commenced without delay, and the cohesion of the anlagen was impaired. Ca++-free medium duplicated the effects of CMF, but neither Mg++-free medium nor CMF treatment before treatment with protease stimulated migration and reduced cohesion. Precocious neural crest cell migration and reduced cohesion also followed when neural anlagen of pre-migratory stages were cultured with membrane Ca++-channel antagonists D600 and Nifedipine, without any exernal Ca++-depletion. The decrease of cohesion of these tissues is consistent with results in other systems where protease/Ca++-depletion inactivates Ca++-dependent cell-cell adhesive mechanisms. Therefore, we suggest that Ca++-dependent cell-cell adhesions play a part in preventing neural crest cells from migrating precociously and that the timed inactivation of this adhesion system normally helps trigger the onset of migration. The results with blockers of Ca++-channels suggest that Ca++ levels may be involved in regulating this system.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Newgreen DF,Gooday Ddoi
10.1007/BF00218011subject
Has Abstractpub_date
1985-01-01 00:00:00pages
329-36issue
2eissn
0302-766Xissn
1432-0878journal_volume
239pub_type
杂志文章abstract：:Two types of mitochondria-rich cells were found in the interplatelet areas of the gills of the migrating river lamprey. Both cell types are thought to be responsible for ion-transport across the gills. In the fresh-run migrant the gills are dominated by large, flask-shaped cells which show some ultrastructural similar...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221380
更新日期：1976-10-06 00:00:00
abstract：:The present study continues a previous investigation on the median eminence (EM) (Krisch et al., 1978). In rats with high levels of neurohormones (LHRH, vasopressin) a limited immunohistochemical labeling of perivascular tanycyte processes can be observed surrounding capillaries in the marginal region of the organum v...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233891
更新日期：1978-12-29 00:00:00
abstract：:The present study describes substance P-like immunoreactivity in the ciliary ganglia of monkey (Macaca fascicularis) and cat. About 60% of neurons in the monkey ciliary ganglion and 40% in the cat ciliary ganglion were substance P-like immunoreactive, ranging from faint to moderate staining. Substance P-like immunorea...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00354796
更新日期：1994-04-01 00:00:00
abstract：:This study aims to illustrate potential transport mechanisms behind the divergent approaches to nitrogen excretion seen in the ureotelic toadfish (Opsanus beta) and the ammoniotelic plainfin midshipman (Porichthys notatus). Specifically, we wish to confirm the expression of a urea transporter (UT), which is found in t...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1591-0
更新日期：2013-06-01 00:00:00
abstract：:In the developing mammalian nervous system, common progenitors integrate both cell extrinsic and intrinsic regulatory programs to produce distinct neuronal and glial cell types as development proceeds. This spatiotemporal restriction of neural progenitor differentiation is enforced, in part, by the dynamic reorganizat...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-014-2011-9
更新日期：2015-01-01 00:00:00
abstract：:The presence of melatonin is demonstrated in the pineal gland, the retina and the Harderian gland in some mammalian and non-mammalian vertebrates, using a specific fluorescence labelled antibody technique. Four different potent antibodies against melatonin have been used and compared. In the pineal gland of hamsters, ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233830
更新日期：1981-01-01 00:00:00
abstract：:Human endothelial progenitor cells (EPCs) were isolated from cord blood samples and enriched by magnetic activated cell sorting method based on the CD133 marker. Cells were incubated with different doses of bacterial lipopolysaccharide, ranging from 2, 5, 10, 50, 100, 200, 250, 500, to 1000 µg/ml, for 48 h. The cell s...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03405-4
更新日期：2021-01-18 00:00:00
abstract：:We used a combined biochemical and histocytochemical approach to study ecto-ATPase in the rat cardiac muscle. The reaction medium employed for histocytochemical detection was optimized in biochemical assays to achieve the highest enzyme activity and lowest inhibition by the capture agent used for visualization of the ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004419900105
更新日期：1999-12-01 00:00:00
abstract：:Survival of mesenchymal stem cells (MSCs) against oxidative stress and inflammation is vital for effective stem cell therapy. The reactive oxygen species (ROS) result in apoptosis and release of inflammatory mediators. Adipose-derived stem cells (ASCs) have shown promise for stem cell therapy owing to their anti-infla...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2857-3
更新日期：2018-10-01 00:00:00
abstract：:In contrast to the vitellolysis zone which is involved in the degradation of the yolk, the cytoplasmic zone of the yolk syncytial layer, composed of many cytoplasmic organelles, is implicated in a secretory process. The granular endoplasmic reticulum of the latter is extremely well developed and organized into trabecu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236349
更新日期：1979-01-30 00:00:00
abstract：:The choroid plexus from the lateral ventricles of 18-day chick embryos was cultivated as an organ in medium 199 until the degeneration of the stroma. Selected plexuses forming an empty epithelial sac were then incubated with enzyme-treated human immunoglobulin (5S-antibody) and with native human immunoglobulin (7S-ant...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00237886
更新日期：1980-01-01 00:00:00
abstract：:Loss of wild-type p53 activity is thought to be a major predictor of failure to respond to radiotherapy and chemotherapy in various human cancers. This assumption is largely based on some cell-death studies in p53-knockout mice and on correlations of p53 status assessed by immunochemistry or single-strand conformation...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410051072
更新日期：1998-06-01 00:00:00
abstract：:Nerve fibers connecting the brain with the pineal gland of the Mongolian gerbil (central pinealopetal fibers) were investigated by means of light and electron microscopy. Several myelinated fibers penetrate from the brain into the deep pineal gland, extend further into the pineal stalk and continue to the superficial ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213804
更新日期：1983-01-01 00:00:00
abstract：:Enzymatic activities of calcium-magnesium dependent adenosine triphosphatase (Ca-ATPase) and nonspecific alkaline phosphatase (ALPase) were localized at the initial calcification sites of dentin and enamel of rat incisor teeth using electron-microscopic cytochemistry. Ca-ATPase was localized in the Golgi cisternae, cy...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221856
更新日期：1986-01-01 00:00:00
abstract：:Differentiated nongranulated (folliculo-stellate) cells were observed in the centre and periphery of the cords of cells in the pars distalis of the adult tammar wallaby (Macropus eugenii). The nongranulated cells formed follicles containing a small lumen: the cell apices were joined by junctional complexes including z...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210898
更新日期：1982-01-01 00:00:00
abstract：:More than twenty different enzyme activities of fractions containing dictyosome-like structures (DLS) as a dominant cell component were monitored. Plasma membrane vesicles were a major contaminant of the DLS fractions, which, presumably as a consequence, were enriched somewhat in plasma membrane markers. The lysosomal...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217555
更新日期：1985-01-01 00:00:00
abstract：:Andersen's syndrome (AS) is a rare autosomal disorder that has been defined by the triad of periodic paralysis, cardiac arrhythmia, and developmental anomalies. AS has been directly linked to over 40 different autosomal dominant negative loss-of-function mutations in the KCNJ2 gene, encoding for the tetrameric strong ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-017-2696-7
更新日期：2018-02-01 00:00:00
abstract：:The insect midgut epithelium represents an interface between the internal and the external environment and it is the almost unique epithelial tissue by which these arthropods acquire nutrients. This epithelium is indeed able to produce digestive enzymes and to support vectorial transport of small organic nutrients, io...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-019-03076-w
更新日期：2019-09-01 00:00:00
abstract：:A tumor model system of clones of myeloproliferative sarcoma virus (MPV)-transformed rat fibroblasts (NRK) with different growth properties and metastatic potential was studied. The relationship between metastatic behavior and composition of carbohydrate-binding proteins (lectins) was analyzed by affinity chromatograp...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215191
更新日期：1986-01-01 00:00:00
abstract：:Exposure to maternal diabetes during fetal growth is a risk factor for the development of type II diabetes (T2D) in later life. Discovery of the mechanisms involved in this association should provide valuable background for therapeutic treatments. Early embryogenesis involves epigenetic changes including histone modif...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-017-2583-2
更新日期：2017-06-01 00:00:00
abstract：:The processes involved in the regulation of bone cell metabolism are complex, including those implicated in bone cell coupling. This study was undertaken to develop a model that would permit real-time interaction between osteoclastic cells and osteoblasts in vitro. Osteogenic bone marrow stromal cells were isolated fr...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051160
更新日期：1998-10-01 00:00:00
abstract：:Serial sections of human vaginal and keratinized oral-gingival epithelia were investigated for ciliary structures. Most melanocytes of the gingival epithelium lacked cilia, whereas almost all basal keratinocytes of the deeper portion of the epithelial ridges possessed one cilium each. In the suprabasal layers of the r...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215830
更新日期：1988-02-01 00:00:00
abstract：:Pituitary adenylate cyclase-activating polypeptide (PACAP) and helospectin are two vasoactive intestinal polypeptide (VIP)-related neuropeptides that have recently been demonstrated in the mammalian gut; the aim of this study was to reveal their occurrence and localisation in the gastrointestinal tract, swimbladder, u...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00300227
更新日期：1994-09-01 00:00:00
abstract：:Bullous pemphigoid antigen-1 (BPA1) and alpha(6)beta(4)-integrin colocalize at the hemidesmosomes in basal-layer keratinocytes of normal squamous epithelia. The expression of these genes was analyzed during the process of tumor cell invasion and metastasis on frozen sections of head and neck biopsies, and the structur...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100462
更新日期：2001-12-01 00:00:00
abstract：:The European shore crab Carcinus maenas and the common hermit crab Pagurus bernhardus are members of the sister taxa Brachyura and Anomura (together forming the taxon Meiura) respectively. Both species share similar coastal marine habitats and thus are confronted with similar environmental conditions. This study sets ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1353-4
更新日期：2012-04-01 00:00:00
abstract：:In the present study, immunogold labeling of ultrathin sections of rat small intestine and liver has been used to obtain insights into the ultrastructural localization and possible functions of annexins. In enterocytes, annexins II, IV, and VI are found at the periphery of the core of each microvillus and of the rootl...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00414181
更新日期：1994-11-01 00:00:00
abstract：:Using histochemical, immunohistochemical and biochemical techniques, noradrenaline-, neuropeptide Y-, vasoactive intestinal polypeptide-, substance P- and calcitonin gene-related peptide-containing nerve fibres were studied in the uterine artery of virgin, progesterone-treated and pregnant guinea-pigs. Morphological c...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF01740777
更新日期：1990-03-01 00:00:00
abstract：:The fine structure of the ventrolateral and dorsomedial subdivisions of the ventromedial nucleus (VMN) of the hypothalamus was examined in ovariectomized/control and ovariectomized/estrogen-treated rats to compare neurons of these areas to other neurons (specifically the ventrolateral thalamus), and to determine the e...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219357
更新日期：1981-01-01 00:00:00
abstract：:Captopril [1-(D-3-mercapto-2-methyl-1-oxopropyl)-L-proline (S,S)] is now known to be an inhibitor of angiotensin-converting enzyme. In male mice, the juxtaglomerular cells were examined by electron microscopy 42 h after administration of captopril. Numerous dense granules appeared in the rough endoplasmic reticulum of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209980
更新日期：1981-01-01 00:00:00
abstract：:The ultrastructural localisation of acid phosphatases (AcPhs) during the normal daily breakdown of rhabdomere membrane in Dinopis has been examined using beta-glycerophosphate and p-nitrophenyl phosphate as substrates. Results are related to the classification of organelles in the receptors given by Blest, Powell and ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236082
更新日期：1979-07-17 00:00:00
