Abstract：
:This study aims to illustrate potential transport mechanisms behind the divergent approaches to nitrogen excretion seen in the ureotelic toadfish (Opsanus beta) and the ammoniotelic plainfin midshipman (Porichthys notatus). Specifically, we wish to confirm the expression of a urea transporter (UT), which is found in the gill of the toadfish and which is responsible for the unique "pulsing" nature of urea excretion and to localize the transporter within specific gill cells and at specific cellular locations. Additionally, the localization of ammonia transporters (Rhesus glycoproteins; Rhs) within the gill of both the toadfish and midshipman was explored. Toadfish UT (tUT) was found within Na(+)-K(+)-ATPase (NKA)-enriched cells, i.e., ionocytes (probably mitochondria-rich cells), especially along the basolateral membrane and potentially on the apical membrane. In contrast, midshipman UT (pnUT) immunoreactivity did not colocalize with NKA immunoreactivity and was not found along the filaments but instead within the lamellae. The cellular location of Rh proteins was also dissimilar between the two fish species. In toadfish gills, the Rh isoform Rhcg1 was expressed in both NKA-reactive cells and non-reactive cells, whereas Rhbg and Rhcg2 were only expressed in the latter. In contrast, Rhbg, Rhcg1 and Rhcg2 were expressed in both NKA-reactive and non-reactive cells of midshipman gills. In an additional transport epithelium, namely the intestine, the expression of both UTs and Rhs was similar between the two species, with only subtle differences being observed.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Bucking C,Edwards SL,Tickle P,Smith CP,McDonald MD,Walsh PJdoi
10.1007/s00441-013-1591-0subject
Has Abstractpub_date
2013-06-01 00:00:00pages
623-37issue
3eissn
0302-766Xissn
1432-0878journal_volume
352pub_type
杂志文章abstract：:The fine structure, nature and fate of the components of the nucleolus were studied in young (steps 1, 2), intermediate (steps 3, 4, 5) and mature spermatids (steps 6, 7, 8) of man and monkey, by use of several cytochemical techniques (alcoholic PTA; sodium tungstate: EDTA; HAPTA; nuclease-gold complexes; NOR silver s...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00305735
更新日期：1991-04-01 00:00:00
abstract：:The fine structure of Heterophrys marina (Centrohelidia, Heliozoa) is described with special reference to centroplast structure, morphogenesis and "behavior" of kinetocysts (= axopodial granules which perform saltatory movement), and formation of organic spicules in a new type of organelle located in the plasma membra...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222116
更新日期：1975-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00239339
更新日期：1980-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0660-2
更新日期：2008-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00305236
更新日期：1990-12-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1991-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2003-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00313518
更新日期：1990-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-1148-y
更新日期：2005-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1987-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2004-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
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更新日期：2017-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00204789
更新日期：1982-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220439
更新日期：1979-11-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221710
更新日期：1975-06-24 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1130-9
更新日期：2011-05-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00353897
更新日期：1992-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-1889-6
更新日期：2014-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：2009-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1987-10-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1986-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0668-7
更新日期：2008-10-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-014-1996-4
更新日期：2015-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0894-6
更新日期：2004-07-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00338049
更新日期：1992-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-009-0793-y
更新日期：2009-07-01 00:00:00
abstract：:We have recently examined the electrophysiology and ultrastructure of approximately 100 tactile spines from the metathoracic legs of adult cockroaches. In only one animal the single sensory neuron that innervates the spine was replaced with a pair of apparently identical neurons which we believe were both functional. ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216522
更新日期：1984-01-01 00:00:00
