Abstract：
:Mouse milk fat globule-EGF factor 8, MFG-E8, is the ortholog to the human mammary tumor marker, lactadherin, and comprises two spliced variants, the L and S forms. Recent studies have suggested that MFG-E8-L produced by macrophages and Langerhans cells in the skin serves as a linker between phagocytic cells and apoptotic cells, and that MFG-E8-S, also termed SED1, facilitates sperm-egg interaction for fertilization. However, Mfge8 gene expression occurs in various tissues apparently unrelated to these critical events. Our in situ hybridization study has revealed that Mfge8 is expressed in the periderm (the premature epidermis) on embryonic day-14, well before Langerhans cells begin to grow in the prenatal phase. Mfge8 transcript is detectable in the basal and spinous layers throughout skin development, whereas immunostaining has revealed MFG-E8 protein accumulation in the spinous layer. Cultured keratinocyte stem cells consistently express Mfge8-L and -S mRNAs and produce the L protein, which is primarily detectable in the culture supernatant, and the S protein, which is mostly associated with the cells. Upon Ca(2+)-stimulated differentiation, which is detected by a decrease in keratinocyte stem cell marker p63(p51) and the induction of keratin1, we have observed suppression of Mfge8, and the protein becomes localized to the cell-cell borders. Papillomas and carcinomas caused by chronic UV-B irradiation produce MFG-E8 as determined by immunostaining. Thus, undifferentiated and poorly differentiated keratinocytes produce the L and S forms of MFG-E8 during normal and pathological tissue development, probably to support an as yet unidentified membrane function.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Watanabe T,Totsuka R,Miyatani S,Kurata S,Sato S,Katoh I,Kobayashi S,Ikawa Ydoi
10.1007/s00441-005-1148-ykeywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
185-93issue
2eissn
0302-766Xissn
1432-0878journal_volume
321pub_type
杂志文章abstract：:The ultrastructural localisation of acid phosphatases (AcPhs) during the normal daily breakdown of rhabdomere membrane in Dinopis has been examined using beta-glycerophosphate and p-nitrophenyl phosphate as substrates. Results are related to the classification of organelles in the receptors given by Blest, Powell and ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236082
更新日期：1979-07-17 00:00:00
abstract：:MN9, a monoclonal antibody raised against mouse spermatozoa, specifically recognizes the equatorial segment of sperm head in several mammalian species, including humans. Colloidal gold-immuno-electron microscopy of mouse spermatozoa has shown that the antigen is localized in the space between the outer and inner acros...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00645047
更新日期：1992-12-01 00:00:00
abstract：:The retina of the distal and proximal lens-bearing complex ocelli are composed of pigmented sensory cells and long pigmented cells. A ciliary sheath from each sensory cell, together with the processes of long pigmented cells, extends through the vitreous layer as far as the capsule that envelops the lens. Each ciliary...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219415
更新日期：1976-07-30 00:00:00
abstract：:In recent years, data have accumulated suggesting that the role of neurotrophins and Trk receptors may not be limited to the nervous system, and the presence of these substances has been detected in a variety of vertebrate and invertebrate non-nervous tissues. This study was designed to map the expression of immunorea...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000181
更新日期：2000-04-01 00:00:00
abstract：:Elastic cartilage-derived cells cultured two-dimensionally with FGF2 and corticosteroid produce gel-type masses that become mature cartilage when injected into a subcutaneous pocket. This unique method has previously been clinically applied for treatments of nasal augmentation. However, the components of the gel-type ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0438-y
更新日期：2007-09-01 00:00:00
abstract：:Fueled by the discovery of head regeneration in triclads (planarians) two and a half centuries ago, flatworms have been the focus of regeneration research. But not all flatworms can regenerate equally well and to obtain a better picture of the characteristics and evolution of regeneration in flatworms other than plana...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03302-w
更新日期：2020-11-07 00:00:00
abstract：:Transmission electron micrographs of the mesonephric nephron in 18 day rabbit embryos reveal major cytological structures reappearing in the nephron of the definitive rabbit kidney. The initial segment of the proximal tubule resembles (despite quite different cell proportions) the cell picture of the metanephric S2-se...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210102
更新日期：1981-01-01 00:00:00
abstract：:The distribution and nature of 125I-atrial natriuretic peptide binding sites have been examined in the brain and pituitary gland of the toad, Bufo marinus, using tissue section autoradiography, affinity cross-linking and electrophoresis, guanylyl cyclase assays and molecular analysis of natriuretic peptide receptor C ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000266
更新日期：2000-10-01 00:00:00
abstract：:The vascular architecture of the rat testis and spermatic cord was studied by a corrosion cast technique combined with scanning and transmission electron microscopy, and light microscopy. The casts preserve the endothelial impressions of the vessels and enable them to be differentiated into the various vascular compon...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217176
更新日期：1985-01-01 00:00:00
abstract：:Nitric oxide and various neuropeptides in the myenteric plexus regulate esophageal motility. We sought colocalization of nitric oxide synthase and neuropeptides in frozen sections of mid-portion of smooth-muscled opossum esophagus using NADPH-diaphorase activity to mark the synthase and immunoreactivity to detect pept...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00331374
更新日期：1994-12-01 00:00:00
abstract：:An indirect immunofluorescence study of the frozen sections of the spleen of an anuran amphibian, Xenopus laevis, showed that lymphocytes bearing a small amount of immunoglobulin (Ig) were localized mostly in the white pulp of non-immunized toads. There were fewer fluorescent cells in the red pulp. In the toads hyperi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218363
更新日期：1982-01-01 00:00:00
abstract：:This review summarizes findings concerning the unique developmental characteristics of mouse head muscles (mainly the masticatory and tongue muscles) and compares their characteristics with those of other muscles. The developmental origin of the masticatory muscles is the somitomeres, whereas the tongue and other musc...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-005-0019-x
更新日期：2005-11-01 00:00:00
abstract：:Proteins participating in vesicular docking and fusion have been identified in the nervous system. Such proteins appear to be important for the molecular regulation of exocytosis also in non-neuronal cells. The enterochromaffin-like (ECL) cells of the gastric acid-secreting (oxyntic) mucosa secrete histamine and chrom...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050960
更新日期：1997-12-01 00:00:00
abstract：:Galanin immunoreactivity was observed in nerve cell bodies and nerve fibres, but not in enteroendocrine cells, in the small intestine of the guinea-pig. Nerve terminals were found in the myenteric plexus, in the circular muscle, in submucous ganglia, around submucous arterioles, and in the mucosa. Lesion studies showe...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218954
更新日期：1987-12-01 00:00:00
abstract：:The responses of non-neuronal cells to the degeneration of neuroblasts were studied in the spinal ganglia of the chick embryo. Reactive changes of the non-neuronal cells were not apparent during the early stage of degeneration of the neuroblast, but during the later stages of degeneration evidence was found suggesting...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210032
更新日期：1978-06-26 00:00:00
abstract：:In rats the "fast" fibular nerve was transposed to the "slow" soleus muscle outside the original innervation band. Formation of new neuromuscular junctions was induced by cutting the soleus nerve after different periods of time. The morphological maturation of these junctions was studied by electron microscopy. New ne...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215176
更新日期：1976-04-09 00:00:00
abstract：:Examination of pituitaries from young and adult turtles representing four families, reveals that in addition to the abundant juxtaneural pars tuberalis (juxPT) found in this class of reptiles, there is generally a substantial amount of pars tuberalis (PT) tissue closely associated with the pars distalis (PD). The PT f...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218290
更新日期：1982-01-01 00:00:00
abstract：:Light and electron microscopic studies, including the use of the vital dyes trypan blue and acridine orange, indicate that the topical application of precocene II rapidly triggers degenerative processes in the corpora allata (CA) leading within a few days to the virtual disappearance of the parenchymal cells of these ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234326
更新日期：1979-11-01 00:00:00
abstract：:Substance P (SP) is a neuropeptide that is released from axons of sensory neurons and causes signal transduction through the activation of the neurokinin-1 receptor (NK1-R). The present study demonstrates the distribution of SP-like-immunoreactive (SP-LI) axons and the localization of NK1-Rs in rat bone tissue using t...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051100
更新日期：1998-07-01 00:00:00
abstract：:The vertebrate limb is a powerful model system for studying the cellular and molecular interactions that determine morphological pattern during embryonic development. Recent advances in our understanding of these interactions have shed new light on the molecular mechanisms of vertebrate limb development, evolution and...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410051261
更新日期：1999-04-01 00:00:00
abstract：:The H-Tx rat has fetal-onset hydrocephalus associated with closure of the cerebral aqueduct and a reduction in the secretory cells of the subcommissural organ (SCO), a circumventricular organ situated in the dorsal wall of the cerebral aqueduct. The objective of this study was to determine the role of the SCO in hydro...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-003-0843-9
更新日期：2004-03-01 00:00:00
abstract：:Mast cells (MCs) and airway nerves play an important role in allergic asthma. However, little is known about the MCs and their interaction with airway nerves during allergic airway inflammation. This study aims to investigate the distribution and proliferation of MC populations in different lung compartments, along wi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-017-2597-9
更新日期：2017-08-01 00:00:00
abstract：:The effects of radiotherapy on neuropeptide expression in the rat larynx were studied. Irradiation was given for five days, 6 or 8 Gray daily. Ten days after the end of irradiation, the larynx, the laryngeal nerves and different ganglia related to the larynx were dissected out from irradiated and control animals and p...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00300691
更新日期：1995-01-01 00:00:00
abstract：:Enteroendocrine cells containing glucagon-, substance P-, neurotensin- and VIP-like substances have been demonstrated immunocytochemically in the gut of Barbus conchonius. Mainly based on the distribution of the immunoreactive endocrine cells in this and a previous study, at least eight different enteroendocrine cell ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00229200
更新日期：1984-01-01 00:00:00
abstract：:The globiferous pedicellariae of Psammechinus miliaris are described. Two fixation methods giving minimal distortion and rapid tissue hardening were adapted for soft tissue preparation for scanning electron microscopy. The pedicellarial valves are covered by a microvillous epithelium. The outer valve epithelial microv...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220184
更新日期：1975-10-13 00:00:00
abstract：:Transplantation of a small fragment of the ileum beneath the kidney capsule in syngenic mice results in the formation of a cyst lined with proliferating intestinal epithelium. The duration of the cell cycle in the epithelium was determined (using tritiated thymidine and the FLM method) as 14.5 h, as compared with 11.5...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221125
更新日期：1977-02-02 00:00:00
abstract：:Striated rootlets in ciliated cells are conical banded structures composed of longitudinally aligned filaments. The formation of striated rootlets during ciliognesis in the human oviduct epithelium was studied by electron microscopy. Primitive rootlets appeared at the proximal side of basal bodies before or at the sam...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050905
更新日期：1997-10-01 00:00:00
abstract：:Sites of synthesis and release patterns of crustacean hyperglycaemic hormone precursor-related peptide (CPRP) were investigated with those of crustacean hyperglycaemic hormone (cHH), in order to determine whether this precursor-related peptide satisfies certain criteria necessary for its definition as a secretable, ci...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-001-0469-8
更新日期：2002-01-01 00:00:00
abstract：:The antennal lobe is the primary processing center for olfactory information in insects. To understand further the neural circuitry of this brain area, we have investigated the distribution of gamma-aminobutyric acid (GABA) and its colocalization with neuropeptides in the antennal lobe of the noctuid moth Heliothis vi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0744-z
更新日期：2009-03-01 00:00:00
abstract：:We have developed a primary cell culture system of antennal mechanoreceptor neurons from early-stage pupal sphinx moth Manduca sexta. Dissociated neurons from the moth antennae differentiated, grew and survived for several weeks in a conditioned culture medium. Bipolar neurons with soma diameters of 10-25 microns from...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051358
更新日期：1999-08-01 00:00:00
