Abstract:
:The H-Tx rat has fetal-onset hydrocephalus associated with closure of the cerebral aqueduct and a reduction in the secretory cells of the subcommissural organ (SCO), a circumventricular organ situated in the dorsal wall of the cerebral aqueduct. The objective of this study was to determine the role of the SCO in hydrocephalus pathogenesis. Serial brain sections through aqueduct regions containing the SCO from H-Tx rats, together with non-hydrocephalic Fischer F344 rats, were studied at E16, before hydrocephalus onset, at E17, the beginning of onset, and at P0 when the hydrocephalus was overt. Tissues were immunostained by AFRU, an antibody against the SCO glycoprotein, and for the intermediate filament nestin. The area of SCO cells with AFRU immunostaining and the severity of lateral ventricle dilatation were quantified by image analysis. At E16 all fetuses had distinct SCO ependymal cells, open aqueducts and normal lateral ventricles. The H-Tx fetuses fell into two groups with large areas and small areas of AFRU immunoreactivity, all with a full complement of SCO cells. By E17, fetuses with small areas of immunoreactivity had reduced numbers of tall SCO secretory cells, and most had aqueducts closed posteriorly and dilated ventricles. Three additional fetuses with small areas of immunoreactivity had narrow but patent aqueducts and normal ventricles, and another had an open aqueduct and dilated ventricles. At P0, pups previously identified as hydrocephalic had small areas of AFRU immunoreactivity, an aqueduct that was closed anteriorly but open posteriorly, ventricular dilatation, and an absence of SCO secretory cells. The aqueduct even when closed was lined by typical ependymal cells throughout. Decreased nestin immunostaining accompanied the SCO changes. It is concluded that reduced SCO glycoprotein immunoreactivity precedes both aqueduct closure and expansion of the lateral ventricles in the H-Tx rat.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Somera KC,Jones HCdoi
10.1007/s00441-003-0843-9keywords:
subject
Has Abstractpub_date
2004-03-01 00:00:00pages
361-73issue
3eissn
0302-766Xissn
1432-0878journal_volume
315pub_type
杂志文章abstract::Nitric oxide and various neuropeptides in the myenteric plexus regulate esophageal motility. We sought colocalization of nitric oxide synthase and neuropeptides in frozen sections of mid-portion of smooth-muscled opossum esophagus using NADPH-diaphorase activity to mark the synthase and immunoreactivity to detect pept...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00331374
更新日期:1994-12-01 00:00:00
abstract::The supraependymal cells (SEC) are a normal component of the wall of the cerebral ventricles. In the hypothalamic area of the third ventricle they are restricted, in healthy animals, to the ependymal projection of the hypophyseotropic area. Here the SEC show great polymorphism. In addition to bipolar, multipolar and s...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215308
更新日期:1976-05-13 00:00:00
abstract::The number of cells comprising biological structures represents fundamental information in basic anatomy, development, aging, drug tests, pathology and genetic manipulations. Obtaining unbiased estimates of cell numbers, however, was until recently possible only through stereological techniques, which require specific...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-015-2127-6
更新日期:2015-04-01 00:00:00
abstract::In Nassarius reticulatus the nuclei and nucleoli undergo important morphological changes from the zygote to the 16-cell stage. In the zygote and in the trefoil (2-cell) and 4-cell stage, several agranular, fibrillar nucleolus-like bodies 1 mum diameter are present in the interphase nucleus. Granular nucleoli first app...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215313
更新日期:1976-05-13 00:00:00
abstract::Using a somatostatin-gold conjugate of known biological activity, high affinity binding sites for this neuropeptide were visualized at cellular resolution on cultured diencephalic astrocytes and on frozen sections of the rat diencephalon. Binding could be completely suppressed in competition experiments with surplus u...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00318767
更新日期:1991-02-01 00:00:00
abstract::An investigation of the stereospecificity of beta-adrenergic insulin release, its relation to alpha-adrenergic blockade and the adrenergic innervation of the pancreatic islets was performed in the mouse. It was observed that in vivo beta-adrenergic stimulation of insulin release by isopropylnoradrenaline was stereospe...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221602
更新日期:1978-10-06 00:00:00
abstract::Andersen's syndrome (AS) is a rare autosomal disorder that has been defined by the triad of periodic paralysis, cardiac arrhythmia, and developmental anomalies. AS has been directly linked to over 40 different autosomal dominant negative loss-of-function mutations in the KCNJ2 gene, encoding for the tetrameric strong ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-017-2696-7
更新日期:2018-02-01 00:00:00
abstract::Locust lipoproteins (lipophorins) were localized by indirect immunofluorescence- and immunogold labelling in cryosections of dorsolongitudinal flight muscles. Immunolabelling was performed with monoclonal antibodies against apolipoproteins epitopes that are exposed at the surfaces of the lipophorin particles. Both at ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216654
更新日期:1988-06-01 00:00:00
abstract::The fine structure of spermatids has been examined in Calliactis, Protanthea, Gonactinia and Parazoanthus (Cnidaria, Anthozoa). The sperm cells are relatively simple and lack distinct acrosomes. Their nuclei, spherical in the zoanthid, in the actinians are slendertipped cones. Condensation of the chromatin is interpre...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222065
更新日期:1975-01-01 00:00:00
abstract::The spatial localisation of insulin-like growth-factor-binding protein-2 (IGFBP-2) and its mRNA was investigated during larval and post-larval developmental stages of the gilthead seabream ( Sparus aurata) by immunohistochemistry and in situ hybridisation with specific antisera and riboprobes. During larval developmen...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-0952-0
更新日期:2005-01-01 00:00:00
abstract::Prostatic carcinoma affects 1 in 11 men and targets bone with sclerotic metastases. The study of prostate carcinoma growth in bone has been hampered by the lack of suitable animal models. We have developed an in vivo model of prostate carcinoma growth in bone by inoculating three human prostate carcinoma cell lines (P...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-001-0503-x
更新日期:2002-03-01 00:00:00
abstract::During the reproductive period of the male stickleback, structural and functional changes of the kidney take place, both in the glomeruli and in the renal tubule cells. The structural changes in the glomeruli involve almost all glomerular components and point to a reduction of the glomerular filtration rate. The purpo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00210146
更新日期:1981-01-01 00:00:00
abstract::The orderly arrangement of fibroblasts and collagen in tendons and ligaments suggests that these cells may have precise relationships with one another and with the collagen fibrils. The spatial organization of rat tail tendon was therefore examined using scanning and transmission electron microscopy and by reconstruct...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217304
更新日期:1984-01-01 00:00:00
abstract::Homeobox genes encode transcription factors that regulate embryonic development programs including organogenesis, axis formation and limb development. Previously, we identified and cloned a mouse double homeobox gene, Duxbl, whose homeodomain exhibits the highest identity (67 %) to human DUX4, a candidate gene of faci...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-014-1974-x
更新日期:2014-11-01 00:00:00
abstract::Previous light-microscopic and ultra-immunohistochemical tracer studies revealed the existence of an independent local immune response of the simian oral mucosa. This local response is attributed to the presence of minor salivary gland (MSG) duct-related lymphoid tissue. Semithin sections from a total of 263 Epon-embe...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217578
更新日期:1985-01-01 00:00:00
abstract::We have previously shown that mice deficient in the gene for the myelin-associated glycoprotein (MAG) develop normal myelin in the peripheral nerves, but show axon and myelin degeneration at eight months of age, suggesting that MAG is involved in the maintenance of axon-Schwann cell integrity. The search for molecules...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050727
更新日期:1997-01-01 00:00:00
abstract::Fragments of pituitary tissue obtained from a total of 37 patients with either breast cancer, diabetic retinopathy, galactorrhea, or acromegaly were dissociated into single cell suspensions prior to cell culture. Release of human growth hormone (hGH) and human prolactin (hPRL) into the culture medium was measured by r...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219803
更新日期:1978-08-16 00:00:00
abstract::Alcohol and aldehyde dehydrogenases (ADHs and ALDHs) may be of interest in the pathology of Parkinson's disease (PD) because of their role in protection against toxins and in retinoid metabolism, which is required for growth and development of the mesencephalic dopamine system. In the present study, the spatial and te...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-0038-7
更新日期:2005-11-01 00:00:00
abstract::Tenidap is a structurally novel antirheumatic agent with anti-inflammatory and analgesic properties. Previous studies have shown that tenidap is able to inhibit the production and action of cytokines such as interleukin-1, interleukin-6 (IL-6) and tumour necrosis factor alpha. However, the mechanisms by which tenidap ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050810
更新日期:1997-05-01 00:00:00
abstract::More than twenty different enzyme activities of fractions containing dictyosome-like structures (DLS) as a dominant cell component were monitored. Plasma membrane vesicles were a major contaminant of the DLS fractions, which, presumably as a consequence, were enriched somewhat in plasma membrane markers. The lysosomal...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217555
更新日期:1985-01-01 00:00:00
abstract::The glandular lobe of the corpus cardiacum (CC) of Locusta migratoria contains secretory cells and axon terminals. It is generally accepted that the glandular cells produce an adipokinetic hormone which stimulates the release of diglycerides from the fat body. The axon terminals are supposed to be involved in the regu...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00223069
更新日期:1977-10-26 00:00:00
abstract::The ultrastructure of the dermal papulae of a starfish (Asterias rubens) is consistent with a respiratory function. The present study has shown no regions specialized for excretory mechanisms. The papulae consist of an outer ectodermal epithelium of sensory, support and gland cells and a small basiepithelial nerve ple...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00229616
更新日期:1978-03-13 00:00:00
abstract::In the salmon and trout aminergic cell bodies were found in the nucleus recessus lateralis (NRL) and the nucleus recessus posterioris (NRP), both of which are situated near the third ventricle. Three cell types could be distinguished. Type 1 produces a green and type 2 a yellow fluorescence. The former type probably c...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219556
更新日期:1978-07-10 00:00:00
abstract::An immunohistochemical staining technique with the D2-40 antibody was undertaken to examine the functional and morphological features of lymphatic networks in tissue sections and whole-mount preparations of normal nasal mucosa and ethmoid sinus mucosa. In normal nasal mucosa, most lymphatic vessels were found in the s...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0337-7
更新日期:2007-03-01 00:00:00
abstract::The present study continues a previous investigation on the median eminence (EM) (Krisch et al., 1978). In rats with high levels of neurohormones (LHRH, vasopressin) a limited immunohistochemical labeling of perivascular tanycyte processes can be observed surrounding capillaries in the marginal region of the organum v...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233891
更新日期:1978-12-29 00:00:00
abstract::Serial sections of human vaginal and keratinized oral-gingival epithelia were investigated for ciliary structures. Most melanocytes of the gingival epithelium lacked cilia, whereas almost all basal keratinocytes of the deeper portion of the epithelial ridges possessed one cilium each. In the suprabasal layers of the r...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215830
更新日期:1988-02-01 00:00:00
abstract::During developmental critical periods, external stimuli are crucial for information processing, acquisition of new functions or functional recovery after CNS damage. These phenomena depend on the capability of neurons to modify their functional properties and/or their connections, generally defined as "plasticity". Al...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-011-1293-4
更新日期:2012-07-01 00:00:00
abstract::The distribution of neuropeptide Y (NPY)-like immunoreactivity in the brain of the bichir, Polypterus senegalus, was examined immunohistochemically. NPY-like immunoreactivity was distributed widely in the brain, with the highest density in the diencephalon. NPY-positive perikarya were found in various areas, including...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050874
更新日期:1997-08-01 00:00:00
abstract::Immunocytochemistry was used to determine if photoperiod and/or sex have any effect on the pattern of the luteinizing hormone-releasing hormone (LHRH) system in the brain of the Japanese quail. Immunopositive perikarya were found within three major areas of the brain: the rostral paraolfactory lobe, the preoptic, and ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222289
更新日期:1988-08-01 00:00:00
abstract::The European shore crab Carcinus maenas and the common hermit crab Pagurus bernhardus are members of the sister taxa Brachyura and Anomura (together forming the taxon Meiura) respectively. Both species share similar coastal marine habitats and thus are confronted with similar environmental conditions. This study sets ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-012-1353-4
更新日期:2012-04-01 00:00:00