Abstract：
:Fragments of pituitary tissue obtained from a total of 37 patients with either breast cancer, diabetic retinopathy, galactorrhea, or acromegaly were dissociated into single cell suspensions prior to cell culture. Release of human growth hormone (hGH) and human prolactin (hPRL) into the culture medium was measured by radioimmunoassay. During a 3-week culture period, prolactin cells released 9--13 times the intracellular levels of hPRL at the time of seeding, whereas hGH release from growth hormone cells was only 1--2 times that of their initial intracellular level during this same time. Both growth hormone and prolactin cells retained distinctive ultrastructural features during culture. The prolactin cells responded to TRH stimulation by elevated release of PRL into the medium. No evidence for mitotic division of prolactin cells in vitro was found.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Snyder J,Hymer WC,Wilfinger WWdoi
10.1007/BF00219803subject
Has Abstractpub_date
1978-08-16 00:00:00pages
379-88issue
3eissn
0302-766Xissn
1432-0878journal_volume
191pub_type
杂志文章abstract：:LW13K2 cells, a clone of a spontaneously in vitro transformed derivative of embryonic Lewis rat fibroblastic cells, were studied by phase contrast cine-light microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The ruffles found at the advancing edge of cells grown on glass substr...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219798
更新日期：1977-04-07 00:00:00
abstract：:Synexin (annexin VII) is a Ca(2+)- and phospholipid-binding protein which has been proposed to play a role in Ca(2+)-dependent membrane fusion processes. Using a monoclonal antibody against synexin, Mab 10E7, and immunogold, we carried out a semiquantitative localization study of synexin in bovine adrenal medullary ch...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00319624
更新日期：1992-08-01 00:00:00
abstract：:The distribution of gap junctions (nexuses) in various parts of the small and large intestines of the guinea-pig was studied using the freeze-fracture technique and in thin sections. The percentage area of smooth muscle cell surface occupied by gap junctions varies from 0.50% in the circular muscle of the duodenum to ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209987
更新日期：1981-01-01 00:00:00
abstract：:Granular hormone-producing cells and agranular cells (stellate cells, including giant and pericavitary cells) were electron microscopically studied in the hypophysis of the small spotted dogfish (Scyllium canicula) up to several months after hatching. Comparisons of results obtained in embryos, newly-hatched fish and ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221607
更新日期：1978-10-06 00:00:00
abstract：:Whole pigs lenses were fixed, critical-point dried and fractured, and the internal surfaces examined with a scanning electron microscope. At various locations from the equator to the center of the lens four types of fibers can be distinguished. The superficial fibers have small interdigitations. Cortical lens fibers, ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217281
更新日期：1982-01-01 00:00:00
abstract：:The etiologic basis of Parkinson's disease (PD), the second most common age-related neurodegenerative disorder, is unknown. Recent epidemiological and experimental studies indicate that exposure to environmental agents, including a number of agricultural chemicals, may contribute to the pathogenesis of this disorder. ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-004-0937-z
更新日期：2004-10-01 00:00:00
abstract：:Chorionic binucleate cells (BNC) occur in several ruminants including cow, deer, goat and sheep. They migrate through the chorionic tight junction to fuse with uterine epithelial cells and discharge their granules into maternal connective tissue. We have compared the BNC of near-term, resin-embedded, ovine and bovine ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00331380
更新日期：1994-12-01 00:00:00
abstract：:Immunocytochemical application of the antimuscarinic acetylcholine receptor antibody M35 to pancreas tissue revealed the target areas for the parasympathetic nervous system. Immunoreactivity in the endocrine pancreas was much higher than that in the exocrine part. Moreover, the endocrine cells at the periphery of the ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00384730
更新日期：1992-07-01 00:00:00
abstract：:Apoptotic and inflammatory processes occur in human arteriosclerotic lesions. We examined the hypothesis whether both processes are possibly associated by studying the colocalization of corresponding markers. In 11 human arteriosclerotic carotid arteries, proapoptotic markers (CPP32 (caspase-3), poly(ADP-ribose) polym...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0024-0
更新日期：2005-12-01 00:00:00
abstract：:Previous studies have revealed a remarkable capacity of intracerebral grafts of embryonic brain tissue to establish extensive axonal connections with denervated areas in the brains of adult rats. In the present study we have explored the possibilities of using grafts in the spinal cord to substitute for the loss of no...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216024
更新日期：1983-01-01 00:00:00
abstract：:The present study attempts to elucidate the role of TRPV1 cation channel receptor on primary repair in an incision-wounded mouse cornea in vivo. Previous study revealed that blocking TRPV1 suppressed myofibroblast formation and expression of transforming growth factor β1 (TGFβ1) in cultured keratocytes or ocular fibro...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2878-y
更新日期：2018-11-01 00:00:00
abstract：:Caprine preantral follicles within ovarian fragments were cryopreserved in the absence or presence of 0.5 M sucrose with or without 1 M dimethyl sulfoxide and/or 1 M ethylene glycol (EG). After being thawed, they were washed in minimum essential medium with or without 0.3 M sucrose. Histological analysis of follicle i...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0193-5
更新日期：2006-09-01 00:00:00
abstract：:Larvae of Ascidia malaca, both before and after hatching, were treated with L-thyroxine solutions. The effect of the thyroid hormone was to induce the onset of metamorphosis and then to cause the rate at which body reorganization occurred to increase. In treated larvae the resorption of the tail occurred only few hour...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00249213
更新日期：1981-01-01 00:00:00
abstract：:Steroid hormones intervene in the structural and functional regulation of neuronal processes during development and thus determine brain differentiation. The effects of estrogens are mediated by two transcription factors, namely estrogen receptor α (ER-α) and estrogen receptor β (ER-β), that regulate the expression of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-2023-5
更新日期：2015-02-01 00:00:00
abstract：:The fine structural organization of a cortical filament layer in normal locomoting Amoeba proteus was demonstrated using improved fixation and embedding techniques. Best results were obtained after application of PIPES-buffered glutaraldehyde in connection with substances known to prevent the depolymerization of F-act...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215699
更新日期：1982-01-01 00:00:00
abstract：:Teleost fish exhibit extraordinary cognitive skills that are comparable to those of mammals and birds. Kin recognition based on olfactory and visual imprinting requires neuronal circuits that were assumed to be necessarily dependent on the interaction of mammalian amygdala, hippocampus, and isocortex, the latter being...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-020-03378-4
更新日期：2021-01-30 00:00:00
abstract：:The inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular Ca2+ release channel responsible for mobilizing stored Ca2+. Three different receptor types have been molecularly cloned, and their genes have been classified into a family. The gene for the type 1 receptor (IP3R1) is predominantly expressed in cereb...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00307790
更新日期：1995-05-01 00:00:00
abstract：:A total of 20 Golgi-impregnated cone horizontal cells of Nannacara anomala (Cichlidae) were studied in alternating semi- and ultrathin sections in order to examine their connections with the overlying square mosaic of equal double and central single cones. Cone horizontal cells exhibiting three types of processes: (a)...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220825
更新日期：1976-11-24 00:00:00
abstract：:In early postnatal rats, immunoreactive LHRH perikarya in the preoptic area were studied by light and electron microscopy. Synaptic junctions were found between the immunoreactive perikaryon or its process, and the immunonegative nerve fibers. The significance of these synapses is discussed in relation to possible mec...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216754
更新日期：1981-01-01 00:00:00
abstract：:During amphibian intestinal remodeling, thyroid hormone (TH) induces some larval epithelial cells to dedifferentiate into adult stem cells, which newly generate the absorptive epithelium analogous to the mammalian epithelium. To clarify molecular mechanisms underlying adult epithelial development, we here focus on TH ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-016-2396-8
更新日期：2016-08-01 00:00:00
abstract：:The peritoneal mesothelium of mouse embryos (12 to 18 day of gestation) was studied by freeze-fracture and in sections in order to reveal the initial formation of the tight junctions. Freeze-fracture observations showed three types of tight junctions. Type I consists of belt-like meshworks of elevations on the P face ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00232008
更新日期：1979-05-18 00:00:00
abstract：:Pancreatic acinar cells of euthermic, hibernating and arousing individuals of the hazel dormouse Muscardinus avellanarius (Gliridae) have been observed at the electron-microscopic level and analysed by means of ultrastructural morphometry and immunocytochemistry in order to investigate possible fine structural changes...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051082
更新日期：1998-06-01 00:00:00
abstract：:Electron-microscopic evidence indicates that during conditions of high hormone demand such as lactation there is a dramatic reduction in the number of fine glial processes which are normally interposed between magnocellular neuroendocrine cell somata in the supraoptic nucleus (SON). The purpose of this study was to co...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00225557
更新日期：1985-01-01 00:00:00
abstract：:In amniotes, the developmental potentials of neural crest cells differ between the cranium and the trunk. These differences may be attributable to the different expression patterns of Hox genes between cranial and trunk neural crest cells. However, little is known about the factors that control Hox genes expression in...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-009-0827-5
更新日期：2009-09-01 00:00:00
abstract：:We have developed a simple and reliable method of preserving antigen immunoreactivity with concomitant excellent retention of the cell ultrastructure. Using this method, we have been able to follow the origin and developmental stages of nuage accumulations within the nurse cell/oocyte syncytium in the ovary of the fru...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1145-2
更新日期：2011-04-01 00:00:00
abstract：:Gap junctions exist in the septa between the segments of the lateral giant axons in the ventral nerve cord of the crayfish Procambarus. A large increase in the resistance (uncoupling) of these gap junctions was brought about by mechanical injury to the axonal segments. Both thin sections and freeze-fracture preparatio...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217847
更新日期：1984-01-01 00:00:00
abstract：:Ossification of calvariae from day-21 rat fetuses was reinvestigated by electron microscopy using different fixation techniques (glutaraldehyde/OsO4, tannic acid, ruthenium red, K-pyroantimonate). An osteoid layer with scattered mineral deposits was found at the mineralization front. Directly beyond this layer, a shee...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318693
更新日期：1992-01-01 00:00:00
abstract：:The neuropeptide content of nerve fibers associated with submucosal arteries in the small intestine of guinea pigs was studied in whole-mount preparations using immunohistochemical methods. Tissues were obtained from normal animals or animals in which the small intestine had been extrinsically denervated. In normal an...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219756
更新日期：1988-09-01 00:00:00
abstract：:During metamorphosis, the frog intestine goes through a dramatic shortening with extensive apoptosis and regeneration in the epithelial layer and connective tissue. Our aim was to study changes in the enteric nervous system represented by one inhibitory (vasoactive intestinal polypeptide; VIP) and one excitatory (subs...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0856-z
更新日期：2004-04-01 00:00:00
abstract：:The transient receptor potential (TRP) superfamily comprises a group of non-selective cation channels that have been implicated in both receptor and store-operated channel functions. The family of the classical TRPs (TRPCs) consists of seven members (TRPC1-7). The presence of TRPC1 and TRPC5 mRNA in the brain has prev...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0004-4
更新日期：2005-11-01 00:00:00
