Abstract：
:Synexin (annexin VII) is a Ca(2+)- and phospholipid-binding protein which has been proposed to play a role in Ca(2+)-dependent membrane fusion processes. Using a monoclonal antibody against synexin, Mab 10E7, and immunogold, we carried out a semiquantitative localization study of synexin in bovine adrenal medullary chromaffin granules, and in resting and nicotine-stimulated adrenal chromaffin cells. Isolated chromaffin granules contained very little synexin, whereas chromaffin granules aggregated with synexin (24 micrograms/mg) and Ca2+ (1 mM) clearly showed synexin-associated immunogold particles in the vicinity of the granule membrane (1.88 gold particles per granule profile). In isolated, cultured adrenal chromaffin cells, synexin was present in the nucleus (5.5 particles/microns 2) and in the cytosol (5.3 particles/microns 2), but mainly around the granule membrane in the granular cell area (11.7 particles/microns 2). During the active phase of cholinergically stimulated catecholamine secretion, the amount of synexin label was reduced by 33% in the nucleus, by 23% in the cytosol, and by 51% in the granule area. The plasma membrane contained a small amount of synexin, which did not significantly change upon stimulation of the cells. We conclude that synexin is involved in the secretory process in chromaffin cells.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Kuijpers GA,Lee G,Pollard HBdoi
10.1007/BF00319624keywords:
subject
Has Abstractpub_date
1992-08-01 00:00:00pages
323-30issue
2eissn
0302-766Xissn
1432-0878journal_volume
269pub_type
杂志文章abstract：:The effects of progesterone pretreatment on in vivo 3H-estrogen uptake by five anterior pituitary cell types was analyzed by means of a quantitative autoradiographic-immunocytochemical technique. Male and female rats castrated for 14 days show nuclear concentration of label in all five cell types one h after injection...
journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：1983-01-01 00:00:00
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更新日期：1986-01-01 00:00:00
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：1995-01-01 00:00:00
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doi：10.1007/BF00218114
更新日期：1983-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327750
更新日期：1990-10-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00571438
更新日期：1990-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220136
更新日期：1976-02-25 00:00:00
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journal_title：Cell and tissue research
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更新日期：1995-10-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1992-01-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：2019-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1988-09-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1993-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1995-05-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：2003-10-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2005-05-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2004-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00340875
更新日期：1991-09-01 00:00:00
