Degeneration of osteoblasts involved in intramembranous ossification of fetal rat calvaria.

Abstract:

:Ossification of calvariae from day-21 rat fetuses was reinvestigated by electron microscopy using different fixation techniques (glutaraldehyde/OsO4, tannic acid, ruthenium red, K-pyroantimonate). An osteoid layer with scattered mineral deposits was found at the mineralization front. Directly beyond this layer, a sheet of one to two layers of necrotic and degenerating osteoblasts was present. Above this sheet, normal and healthy cells were seen, formed by six to eight layers of flattened cells, embedded in a collagenous matrix. The osteoblasts on the less mineralizing opposite side of the calcified calvariae and the osteocytes embedded in the calcified calvariae appeared healthy. Closer inspection of the necrotic zone revealed apatite crystals in vesicles which most probably originated from mitochondria of the degenerated cells. Large K-pyroantimonate deposits were found throughout the osteoid and the necrotic zone, whereas only small granules were scattered in the cytoplasm and at the plasma membrane of the healthy cells directly adjacent to the necrotic zone. A concept of intramembranous mineralization is outlined, according to which osteoblasts store enormous amounts of calcium, which are liberated by physiological cell death in the vicinity of the mineralizing front.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Zimmermann B

doi

10.1007/BF00318693

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

75-84

issue

1

eissn

0302-766X

issn

1432-0878

journal_volume

267

pub_type

杂志文章
  • Expression of neuronal markers suggests heterogeneity of chick sympathoadrenal cells prior to invasion of the adrenal anlagen.

    abstract::We have analyzed the distribution of neural crest-derived precursors and the expression of catecholaminergic and neuronal markers in developing adrenal tissue of chick embryos. Undifferentiated neural crest cells are found in presumptive adrenal regions from embryonic day 3 (E3) onward. An increasing proportion of cel...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-004-0996-1

    authors: Ernsberger U,Esposito L,Partimo S,Huber K,Franke A,Bixby JL,Kalcheim C,Unsicker K

    更新日期:2005-01-01 00:00:00

  • Immunocytochemical detection of mitochondria-rich cells in the brood pouch epithelium of the pipefish, Syngnathus schlegeli: structural comparison with mitochondria-rich cells in the gills and larval epidermis.

    abstract::The brood pouch of the male pipefish (Syngnathus schlegeli) is a ventral organ located on the tail, with the anterior region closely associated with the genital pore. The embryos in the pouch are attached to highly vascularized placenta-like tissue which seals the pouch folds from inside during incubation. The epithel...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410051220

    authors: Watanabe S,Kaneko T,Watanabe Y

    更新日期:1999-01-01 00:00:00

  • Immuno-histochemical localization of LH-RH during different phases of estrus cycle of rat, with reference to the preoptic and arcuate neurons, and the ependymal cells.

    abstract::Immunohistochemical localization of luteinizing hormone-releasing hormone (LH-RH), during different phases of the estrus cycle, in the preoptic, suprachiasmatic and arcuate nuclei, and in the OVLT of rats, with special emphasis on the ependymal cells, was studied by light, fluorescent and electron microscopy, by using...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00221371

    authors: Naik DV

    更新日期:1976-10-06 00:00:00

  • The fine structure and innervation of gill lamellae in Anodonta.

    abstract::Gill lamellae of a bivalve Anodonta woodiana lauta (v. Martens) were observed by electron microscopy. The Anodonta gill wall consists of a single layer of epithelial cells, its basal lamina and the underlying connective tissue layer. It was confirmed that there is no true endothelium in the vessels and that the connec...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00222069

    authors: Nakao T

    更新日期:1975-01-01 00:00:00

  • Lack of adrenomedullin affects growth and differentiation of adult neural stem/progenitor cells.

    abstract::Adrenomedullin (AM) is a peptide hormone involved in the modulation of cellular growth, migration, apoptosis, and angiogenesis. These characteristics suggest that AM is involved in the control of neural stem/progenitor cell (NSPC) biology. To explore this hypothesis, we have obtained NSPC from the olfactory bulb of ad...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-010-0934-3

    authors: Vergaño-Vera E,Fernández AP,Hurtado-Chong A,Vicario-Abejón C,Martínez A

    更新日期:2010-04-01 00:00:00

  • Ultrastructural study on hepatic melanin in Xenopus laevis.

    abstract::The livers of Xenopus laevis, grouped by chronological age (0.5, 2 and 3 yrs), were studied electron microscopically. Ultrastructurally most of the melanin granules in the mature female liver showed an internal structure similar to the melanin granules of the oocytes. The hepatic melanin granules of immature females a...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00220293

    authors: Noda K,Nomaguchi T,Tanaka Y

    更新日期:1977-12-19 00:00:00

  • Some evidence for the ampullary organs in the European cave salamander Proteus anguinus (Urodela, Amphibia).

    abstract::The multicellular epithelial organs in Proteus anguinus, which Bugnion (1873) assumed to be developing neuromasts, have been analyzed by light- and electron-microscopy. Their fundamental structure consists of single ampullae with sensory and accessory cells with apical parts that extend into the pit of the ampulla, an...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00217865

    authors: Istenic L,Bulog B

    更新日期:1984-01-01 00:00:00

  • Apoptosis in cardiac development.

    abstract::Cell degeneration, as a phenomenon accompanying developmental processes, was originally described over a century ago. Apoptosis, a term introduced approximately three decades ago, has occupied investigators particularly with respect to cell and tissue kinetics, emphasizing its role in the disposal of supernumerary, ma...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s004410000227

    authors: Poelmann RE,Molin D,Wisse LJ,Gittenberger-de Groot AC

    更新日期:2000-07-01 00:00:00

  • Stem cells and tooth tissue engineering.

    abstract::The notion that teeth contain stem cells is based on the well-known repairing ability of dentin after injury. Dental stem cells have been isolated according to their anatomical locations, colony-forming ability, expression of stem cell markers, and regeneration of pulp/dentin structures in vivo. These dental-derived s...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s00441-007-0467-6

    authors: Yen AH,Sharpe PT

    更新日期:2008-01-01 00:00:00

  • The mouse pubic symphysis as a remodeling system: morphometrical analysis of proliferation and cell death during pregnancy, partus and postpartum.

    abstract::Marked changes in mice pubic symphysis occur by the end of pregnancy. Tissue remodeling involves a dynamic balance between cell proliferation and programmed cell death as well as changes in the extracellular matrix components. Therefore, it is important to consider both of these cellular behaviors when investigating t...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-007-0463-x

    authors: Veridiano AM,Garcia EA,Pinheiro MC,Nishimori FY,Toledo OM,Joazeiro PP

    更新日期:2007-10-01 00:00:00

  • Autologous chondrocytes as a novel source for neo-chondrogenesis in haemophiliacs.

    abstract::Haemophilic arthropathy is the major cause of disability in patients with haemophilia and, despite prophylaxis with coagulation factor concentrates, some patients still develop articular complications. We evaluate the feasibility of a tissue engineering approach to improve current clinical strategies for cartilage reg...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-016-2408-8

    authors: Stocco E,Barbon S,Radossi P,Rajendran S,Dalzoppo D,Bortolami M,Bagno A,Grandi F,Gamba PG,Parnigotto PP,Tagariello G,Grandi C

    更新日期:2016-10-01 00:00:00

  • A study of TSH-synthesis of spontaneous hypertensive rats by electron microscopic morphometry and autoradiography.

    abstract::In order to compare the functional state of the anterior pituitary of spontaneously hypertensive rats (SHR) with that of nonmotensive Wistar Kyoto rats (WKR), the anterior pituitary was examined by morphometry and autoradiography at the level of electron microscopy. The relative number and the relative volume of thyro...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00237005

    authors: Fujiwara T

    更新日期:1979-10-01 00:00:00

  • Expression of PU.1 and terminal differentiation of alveolar macrophages in newborn rats.

    abstract::PU.1, which is a transcription factor, promotes the terminal differentiation of alveolar macrophages (AMs). Its expression is regulated by granulocyte/macrophage colony-stimulating factor (GM-CSF). In this study of AMs in newborn rats, we performed immunohistochemical staining, acid phosphatase staining, reverse trans...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-007-0405-7

    authors: Iwabuchi H,Kawasaki T,Yamamoto T,Uchiyama M,Nakata K,Naito M

    更新日期:2007-07-01 00:00:00

  • Fibronectin and its relation to the basal lamina and to the cell surface in the chicken blastoderm.

    abstract::The ultrastructural distribution of fibronectin immunoreactivity was investigated in the chicken embryo during late gastrulation. Sites of binding of anti-fibronectin antibodies were ascribed to the basal lamina and associated structures, and to the cell surface. The fibronectin-rich basal lamina was resolved into a l...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00217185

    authors: Harrisson F,Vanroelen C,Vakaet L

    更新日期:1985-01-01 00:00:00

  • Centrosome inheritance in the parthenogenetic egg of the collembolan Folsomia candida.

    abstract::Unfertilized eggs commonly lack centrioles, which are usually provided by the male gamete at fertilization, and are unable to assemble functional reproducing centrosomes. However, some insect species lay eggs that develop to adulthood without a contribution from sperm. We report that the oocyte of the parthenogenetic ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-006-0253-x

    authors: Riparbelli MG,Giordano R,Callaini G

    更新日期:2006-12-01 00:00:00

  • Inhibition of p53 during physiological angiogenesis in the hamster ovary does not affect extent of new vessel formation but delays vessel maturation.

    abstract::Transcription factor p53 regulates the cell cycle and apoptosis and may impair angiogenesis by the deregulation of pro-angiogenic factors and the activation of anti-angiogenic factors. Our aim has been to elucidate further the role of p53 in physiological angiogenesis. By treating hamsters with the wildtype p53 inhibi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-005-1078-8

    authors: Bordel R,Laschke MW,Menger MD,Vollmar B

    更新日期:2005-06-01 00:00:00

  • Morphological characterization of single fan-shaped body neurons in Drosophila melanogaster.

    abstract::The fan-shaped body is the largest substructure of the central complex in Drosophila melanogaster. Two groups of large-field neurons that innervate the fan-shaped body, viz., F1 and F5 neurons, have recently been found to be involved in visual pattern memory for "contour orientation" and "elevation" in a rut-dependent...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-009-0781-2

    authors: Li W,Pan Y,Wang Z,Gong H,Gong Z,Liu L

    更新日期:2009-06-01 00:00:00

  • Differentiation of human skeletal muscle cells in culture: maturation as indicated by titin and desmin striation.

    abstract::This report describes a phenotyping study of differentiating human skeletal muscle cells in tissue culture. Satellite cells (adult myoblasts), isolated from biopsy material, showed a proliferative behaviour in high-nutrition medium, but fused to form myotubes when grown in low-nutrition medium. The expression and stru...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00381893

    authors: van der Ven PF,Schaart G,Jap PH,Sengers RC,Stadhouders AM,Ramaekers FC

    更新日期:1992-10-01 00:00:00

  • Occurrence of nuage in fetal human germ cells.

    abstract::Germ cells of human fetuses aged 7-13 weeks were studied. Dense fibrous material called nuage was found in the cytoplasm of human fetal germ cells. The nuage increased in frequency in the germ cells during the interval studied. In oogonia the nuage occurred much more frequently than in spermatogonia and the frequency ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00209243

    authors: Kellokumpu-Lehtinen PL,Söderström KO

    更新日期:1978-11-09 00:00:00

  • Neuroendocrine cells within colorectal tumours induced by dimethylhydrazine. An immunocytochemical study.

    abstract::Colorectal adenocarcinomas were induced in male Wistar rats, by weekly subcutaneous administration of 1,2-dimethylhydrazine, classified according to the degree of differentiation and submitted to immunocytochemistry for the peptides cholecystokinin (CCK), gastrin, gastric inhibitory polypeptide (GIP), glucagon, neurot...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00219019

    authors: Johnston CF,O'Neill AB,O'Hare MM,Buchanan KD

    更新日期:1986-01-01 00:00:00

  • Dictyosome-like structures from guinea-pig testes lack galactosyltransferase, a Golgi apparatus marker.

    abstract::More than twenty different enzyme activities of fractions containing dictyosome-like structures (DLS) as a dominant cell component were monitored. Plasma membrane vesicles were a major contaminant of the DLS fractions, which, presumably as a consequence, were enriched somewhat in plasma membrane markers. The lysosomal...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00217555

    authors: Morré DJ,Matyas GR,Mollenhauer HH

    更新日期:1985-01-01 00:00:00

  • Appearance of tyrosine hydroxylase, aromatic amino-acid decarboxylase, dopamine beta-hydroxylase and phenylethanolamine N-methyltransferase during the ontogenesis of the adrenal medulla: an immunohistochemical study in the rat.

    abstract::The cellular localization of the enzymes tyrosine hydroxylase (TH), aromatic amino-acid decarboxylase (or dopa decarboxylase, DDC), dopamine beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) in the adrenal medulla of adult rats and rat fetuses (14th, 17th, 18th, 19th and 21st day) was examined. ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00236882

    authors: Verhofstad AA,Hökfelt T,Goldstein M,Steinbusch HW,Joosten HW

    更新日期:1979-08-03 00:00:00

  • Tissue- and age-dependent expression of the bovine DEFB103 gene and protein.

    abstract::Beta-defensin 103 (DEFB103) shares little homology with 8 other members of the bovine beta-defensin family and in other species DEFB103 protein has diverse functions, including antimicrobial activity, a chemoattractant for dendritic cells, enhancing epithelial wound repair and regulating hair colour. Expression of the...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-015-2258-9

    authors: Mirabzadeh-Ardakani A,Solie J,Gonzalez-Cano P,Schmutz SM,Griebel PJ

    更新日期:2016-02-01 00:00:00

  • Immunocytochemical localization of muscarinic acetylcholine receptors in the rat endocrine pancreas.

    abstract::Immunocytochemical application of the antimuscarinic acetylcholine receptor antibody M35 to pancreas tissue revealed the target areas for the parasympathetic nervous system. Immunoreactivity in the endocrine pancreas was much higher than that in the exocrine part. Moreover, the endocrine cells at the periphery of the ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00384730

    authors: Van der Zee EA,Buwalda B,Strubbe JH,Strosberg AD,Luiten PG

    更新日期:1992-07-01 00:00:00

  • Cytoprotective role of vitamin E in porcine adipose-tissue-derived mesenchymal stem cells against hydrogen-peroxide-induced oxidative stress.

    abstract::Survival of mesenchymal stem cells (MSCs) against oxidative stress and inflammation is vital for effective stem cell therapy. The reactive oxygen species (ROS) result in apoptosis and release of inflammatory mediators. Adipose-derived stem cells (ASCs) have shown promise for stem cell therapy owing to their anti-infla...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-018-2857-3

    authors: Bhatti FUR,Kim SJ,Yi AK,Hasty KA,Cho H

    更新日期:2018-10-01 00:00:00

  • The pigmentary system of planaria. II. Physiology and functional morphology.

    abstract::The pigmentary system of the planaria, Dugesia gonocephala s.l. (Platyhelminthes, Turbellaria, Tricladida), consists of granules contained in chromatophore cells distributed in the parenchyma tissue. The administration of MSH release-inhibiting Factor (M.I.F.) leads to an easily observable general decolouration of the...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00236132

    authors: Palladini G,Medolago-Albani L,Margotta V,Conforti A,Carolei A

    更新日期:1979-06-27 00:00:00

  • Spermiogenesis in the rainbow trout (Salmo gairdneri). An ultrastructural study.

    abstract::In an ultrastructural study on the spermiogenesis of the rainbow trout (Salmo gairdneri R.) four spermatogenetic stages were identified. In young round spermatids, the nuclear chromatin was first heterogeneous (euchromatin and heterochromatin). Subsequently, it became more homogeneous and started to condense in the fo...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00238295

    authors: Billard R

    更新日期:1983-01-01 00:00:00

  • Neuronal differentiation in vitro from precursor cells of regenerating spinal cord of the adult teleost Apteronotus albifrons.

    abstract::This study documents neuronal differentiation in vitro from undifferentiated precursor cells of caudalmost regenerating spinal cord of the teleost Apteronotus albifrons. At 11 days in vitro, cells from the caudalmost tip of the regenerating cord are flat and polygonal in shape, lack neuronal processes and do not stain...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00414166

    authors: Anderson MJ,Rossetto DL,Lorenz LA

    更新日期:1994-11-01 00:00:00

  • Localization of myosin and actin in ocular nonmuscle cells. Immunofluorescence-microscopic, biochemical, and electron-microscopic studies.

    abstract::Myosin and actin were localized by indirect immunofluorescence microscopy using specific antibodies prepared in rabbits against highly purified gizzard myosin and actin. A strong fluorescence staining with both antibodies was observed in rat corneal epithelial cells, anterior lens epithelial cells, rod inner segments,...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00221771

    authors: Drenckhahn D,Gröschel-Stewart U

    更新日期:1977-07-19 00:00:00

  • Fine structure of rabbit ear chondrocytes in vitro and after autotransplantation.

    abstract::Chondrocytes were isolated enzymatically from rabbit ear cartilage, grown in vitro or as autotransplants for 1, 2 or 5 weeks and then examined by transmission electron microscopy. A confluent monolayer formed rapidly in vitro and the cells later grew in multiple overlapping layers, producing thick sheets of cartilagin...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00227600

    authors: Thyberg J,Hinek A

    更新日期:1977-05-31 00:00:00