Abstract:
:Ossification of calvariae from day-21 rat fetuses was reinvestigated by electron microscopy using different fixation techniques (glutaraldehyde/OsO4, tannic acid, ruthenium red, K-pyroantimonate). An osteoid layer with scattered mineral deposits was found at the mineralization front. Directly beyond this layer, a sheet of one to two layers of necrotic and degenerating osteoblasts was present. Above this sheet, normal and healthy cells were seen, formed by six to eight layers of flattened cells, embedded in a collagenous matrix. The osteoblasts on the less mineralizing opposite side of the calcified calvariae and the osteocytes embedded in the calcified calvariae appeared healthy. Closer inspection of the necrotic zone revealed apatite crystals in vesicles which most probably originated from mitochondria of the degenerated cells. Large K-pyroantimonate deposits were found throughout the osteoid and the necrotic zone, whereas only small granules were scattered in the cytoplasm and at the plasma membrane of the healthy cells directly adjacent to the necrotic zone. A concept of intramembranous mineralization is outlined, according to which osteoblasts store enormous amounts of calcium, which are liberated by physiological cell death in the vicinity of the mineralizing front.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Zimmermann Bdoi
10.1007/BF00318693subject
Has Abstractpub_date
1992-01-01 00:00:00pages
75-84issue
1eissn
0302-766Xissn
1432-0878journal_volume
267pub_type
杂志文章abstract::We have analyzed the distribution of neural crest-derived precursors and the expression of catecholaminergic and neuronal markers in developing adrenal tissue of chick embryos. Undifferentiated neural crest cells are found in presumptive adrenal regions from embryonic day 3 (E3) onward. An increasing proportion of cel...
journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222069
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
pub_type: 杂志文章,评审
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journal_title:Cell and tissue research
pub_type: 杂志文章,评审
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217185
更新日期:1985-01-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0253-x
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-1078-8
更新日期:2005-06-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-009-0781-2
更新日期:2009-06-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00381893
更新日期:1992-10-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00209243
更新日期:1978-11-09 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
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更新日期:1986-01-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00384730
更新日期:1992-07-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
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更新日期:1979-06-27 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221771
更新日期:1977-07-19 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00227600
更新日期:1977-05-31 00:00:00