Abstract：
:Enzymatic activities of calcium-magnesium dependent adenosine triphosphatase (Ca-ATPase) and nonspecific alkaline phosphatase (ALPase) were localized at the initial calcification sites of dentin and enamel of rat incisor teeth using electron-microscopic cytochemistry. Ca-ATPase was localized in the Golgi cisternae, cytoplasmic vesicles and along the outer surface of the presecretory and secretory ameloblasts, whereas it was totally absent from the odontoblasts in the pulp. Inversely, ALPase reaction was localized along the outer surface of the odontoblasts, but almost completely absent from the ameloblasts. Diffuse extracellular reactions of both enzymes were distributed throughout the unmineralized fibrous matrix of mantle dentin in which a large number of matrix vesicles were scattered. Both Ca-ATPase and ALPase reactions, which appeared in the matrix vesicles in the process of formation of mantle dentin, became most conspicuous at the site of initial dentin calcification. At this stage, an intense Ca-ATPase reaction also appeared along some of the collagen fibrils adjacent to the reactive matrix vesicles. No ALPase reaction was localized along these Ca-ATPase reactive collagen fibrils. Our observations suggest strongly that Ca-ATPase in the matrix vesicles originates from the inner enamel epithelium and/or preameloblasts whereas ALPase originates from the odontoblasts in the pulp. The importance of the coexistence of both enzymes for the control of initial calcification of dental hard tissues is suggested.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Takano Y,Ozawa H,Crenshaw MAdoi
10.1007/BF00221856subject
Has Abstractpub_date
1986-01-01 00:00:00pages
91-9issue
1eissn
0302-766Xissn
1432-0878journal_volume
243pub_type
杂志文章abstract：:The ultrastructure of the mid-gut cells of female Nasonia vitripennis is described. The mid-gut consists of a uniform, single-cell epithelium. The cells of different gut regions were analysed using morphometric techniques in order to determine any differences in the components. The structure of the cells is described ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221084
更新日期：1977-02-09 00:00:00
abstract：:PC12 pheochromocytoma cells treated with nerve growth factor (NGF) in combination with high concentrations of the activators of adenylate cyclase, forskolin or cholera toxin, become more neuron-like in size than cells treated with NGF or with activators of adenylate cyclase alone. Cells treated simultaneously with NGF...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215430
更新日期：1987-07-01 00:00:00
abstract：:Although the function and effects of many growth factors and extracellular matrix (ECM) molecules have been described for several periodontal tissues in vivo and in vitro, the molecular interactions involved in the communication between cells of the periodontal ligament and the alveolar bone are poorly understood. To ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-1026-z
更新日期：2005-03-01 00:00:00
abstract：:Induction of HIF-1α by oxygen limitation promotes increased phosphorylation and catalytic depression of mitochondrial pyruvate dehydrogenase (PDH) and an enhanced glycolytic poise in cells. Cobalt chloride and desferrioxamine are widely used as mimics for hypoxia because they increase the levels of HIF-1α. We evaluate...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1517-2
更新日期：2013-01-01 00:00:00
abstract：:Following castration, the nucleus of the dark cells of the subcortical portions of the collecting tubules in the rat kidney showed two types of morphological alterations: 1) Decomposition of the nuclear membrane in a circumscribed area. This phenomenon is independent of the sex of the animal and becomes more frequent ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220204
更新日期：1980-01-01 00:00:00
abstract：:We have recently examined the electrophysiology and ultrastructure of approximately 100 tactile spines from the metathoracic legs of adult cockroaches. In only one animal the single sensory neuron that innervates the spine was replaced with a pair of apparently identical neurons which we believe were both functional. ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216522
更新日期：1984-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00304513
更新日期：1995-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327979
更新日期：1993-10-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318366
更新日期：1995-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00300691
更新日期：1995-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00305236
更新日期：1990-12-01 00:00:00
abstract：:The size of various tubes within tubular organs such as the lung, vascular system and kidney must be finely tuned for the optimal delivery of gases, nutrients, waste and cells within the entire organism. Aberrant tube sizes lead to devastating human illnesses, such as polycystic kidney disease, fibrocystic breast dise...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-013-1673-z
更新日期：2013-11-01 00:00:00
abstract：:Calretinin and calbindin-D28k are two calcium-binding proteins that are present in largely different sets of nerve cells in the central nervous system. Their appearance during development of the chick retina was studied by immunohistochemistry and Western blots. The patterns are mature one day before hatching. Each ce...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00313956
更新日期：1991-05-01 00:00:00
abstract：:The rejection of skin allografts by the larval lamprey, Lampetra reissneri, was studied by light- and electron-microscopy, with particular attention to the cell types involved in the reaction. In all allografts, melanophores were destroyed within 20-60 days (the mean survival time, 36 +/- 12 days). Neither the epiderm...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222182
更新日期：1983-01-01 00:00:00
abstract：:Since the advent of transmission electron microscopy of tissues capable of growth and regeneration, cell and developmental biologists have postulated that the undifferentiated cells observed within these tissues represent tissue-specific stem or progenitor cells. However, no studies have addressed the issue of whether...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0641-9
更新日期：2002-12-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318878
更新日期：1995-12-01 00:00:00
abstract：:Although several studies have shown a dysregulation of microRNA (miRNA) expression profiles in cutaneous melanoma, there has been little research on the miRNA machinery itself. In this study, we investigated the mRNA expression profiles of different miRNA machinery components in primary cutaneous malignant melanoma (P...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1446-0
更新日期：2012-10-01 00:00:00
abstract：:alpha-Smooth muscle (alpha SM) actin of endothermic vertebrates is selectively recognized by the monoclonal antibody anti-alpha SM-1. Immunoreactivity to this antibody has been shown to be localized in the NH2-terminal sequence Ac-EEED (Chaponnier et al. 1994). Among terrestrial ectothermic vertebrates, two amphibian ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00417867
更新日期：1995-09-01 00:00:00
abstract：:Enzyme-histochemical methods were used to study the metabolic activity of specialized ependyma of the ventrolateral walls and floor of the third ventricle in young male and female rats during the "critical period" of sexual differentiation of the hypothalamus (one week after birth). Histochemical tests were conducted ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220328
更新日期：1976-10-13 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-03130-7
更新日期：2020-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050884
更新日期：1997-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0878-6
更新日期：2004-05-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00306123
更新日期：1994-05-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00226962
更新日期：1984-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00297553
更新日期：1993-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03241-6
更新日期：2020-11-01 00:00:00
abstract：:The three segments (S1, S2, S3) of the proximal tubule of the rat kidney were investigated, with special reference to lysosomes, after castration, estradiol application, and at the end of pregnancy. Especially in S1 and S2 castration induces an increase of cellular autophagy. The nuclei become smaller; endoplasmic ret...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00212331
更新日期：1978-09-26 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217095
更新日期：1982-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051013
更新日期：1998-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233895
更新日期：1978-12-29 00:00:00
