Abstract:
:A culture technique for the in vitro growth and differentiation of antennal cells of Mamestra brassicae is described. The morphology of the growing cells is described. At least five morphological cell types can be distinguished in the cultures. Neuronal cells (type A) were characterized morphologically and immunocytochemically with anti-HRP staining and could be separated into two subtypes, A1 and A2, on the basis of the size of the cell body. Non-neuronal cells could be divided into four types. Type-B cells consisted of large and flat cells with a veil-like cytoplasm. They usually adhered to each other and formed groups of 20 to 50 cells. Type C were large and flat cells, highly variable in size and shape. They closely resembled insect glial cells in culture. Type-D cells grew thick processes, up to 100 microm long. These elongated processes resembled trichoid hairs, and D cells are most probably trichogen cells. Type E were spindle-shaped cells present in low number in the cultures. A high proportion of similar cells in the hemolymph of pupae suggests that E cells are hemocytes. Pheromone-binding proteins were detected using specific antisera in the medium of 3- to 4-week-old cultures.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Lucas P,Nagnan-Le Meillour Pdoi
10.1007/s004410050884subject
Has Abstractpub_date
1997-08-01 00:00:00pages
375-82issue
2eissn
0302-766Xissn
1432-0878journal_volume
289pub_type
杂志文章abstract::The circadian timing system is a neural network consisting of the hypothalamic suprachiasmatic nucleus, aided by the retina, other hypothalamic nuclei, autonomic regions of the spinal cord, sympathetic ganglia and the pineal gland. Extensive studies conducted over the last two decades have unravelled the principal ite...
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