Primary culture of antennal cells of Mamestra brassicae: morphology of cell types and evidence for biosynthesis of pheromone-binding proteins in vitro.

Abstract:

:A culture technique for the in vitro growth and differentiation of antennal cells of Mamestra brassicae is described. The morphology of the growing cells is described. At least five morphological cell types can be distinguished in the cultures. Neuronal cells (type A) were characterized morphologically and immunocytochemically with anti-HRP staining and could be separated into two subtypes, A1 and A2, on the basis of the size of the cell body. Non-neuronal cells could be divided into four types. Type-B cells consisted of large and flat cells with a veil-like cytoplasm. They usually adhered to each other and formed groups of 20 to 50 cells. Type C were large and flat cells, highly variable in size and shape. They closely resembled insect glial cells in culture. Type-D cells grew thick processes, up to 100 microm long. These elongated processes resembled trichoid hairs, and D cells are most probably trichogen cells. Type E were spindle-shaped cells present in low number in the cultures. A high proportion of similar cells in the hemolymph of pupae suggests that E cells are hemocytes. Pheromone-binding proteins were detected using specific antisera in the medium of 3- to 4-week-old cultures.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Lucas P,Nagnan-Le Meillour P

doi

10.1007/s004410050884

subject

Has Abstract

pub_date

1997-08-01 00:00:00

pages

375-82

issue

2

eissn

0302-766X

issn

1432-0878

journal_volume

289

pub_type

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