Abstract：
:Digital dermatitis (DD), a common ulcerative disease of the bovine foot causing lameness and reducing productivity and animal welfare, is associated with infection by spirochete Treponema bacteria. Topical tetracycline, the most common treatment, has inconsistent cure rates; therefore, new therapeutic options are needed. We compared effects of topical oxytetracycline and vitamin D3 on innate immunity in DD-affected skin. Cows with active DD lesions were treated topically with oxytetracycline or vitamin D3 and skin biopsies were collected from lesions. Tissue samples were examined histologically, transcriptional expression of pro-inflammatory cytokines, Toll-like receptors (TLRs), and host defense peptides assessed, and the presence of specific treponeme species determined. Effects of treatments at a mechanistic level were studied in a human keratinocyte model of treponeme infection. Oxytetracycline promoted hyperplastic scab formation in ulcerated DD lesions and decreased transcriptional expression of Cxcl-8 (neutrophil chemoattractant). Oxytetracycline also reduced numbers of Treponema phagedenis and T. pedis and enhanced Tlr2 mRNA expression. Vitamin D3 did not modify expression of cytokines or Tlrs, or bacterial loads, but enhanced transcription of tracheal antimicrobial peptide (Tap), a key bovine β-defensin. Combing oxytetracycline and vitamin D3 provides complementary clinical benefits in controlling DD through a combination of antimicrobial, immunomodulatory, and pro-healing activities.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Watts KM,Lahiri P,Arrazuria R,De Buck J,Knight CG,Orsel K,Barkema HW,Cobo ERdoi
10.1007/s00441-019-03082-ysubject
Has Abstractpub_date
2020-02-01 00:00:00pages
337-348issue
2eissn
0302-766Xissn
1432-0878pii
10.1007/s00441-019-03082-yjournal_volume
379pub_type
杂志文章abstract：:We report here on cholinergic neurons in the rat hippocampal formation that were identified by immunocytochemistry employing a monoclonal antibody against choline acetyltransferase (ChAT), the acetylcholine-synthesizing enzyme. In general, ChAT-immunoreactive cells were rare, but were observed in all layers of the hip...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215891
更新日期：1986-01-01 00:00:00
abstract：:In adrenalectomized rats the influence of salt loading or salt deprivation on the vasopressin and oxytocin content of the median eminence (ME) and the neural lobe (NL) was studied by means of various methods: morphometric and microphotometric analysis of aldehyde fuchsin-stained sections of ME and NL; immunohistochemi...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/BF00215904
更新日期：1986-01-01 00:00:00
abstract：:Four groups of male rats were exercised for periods of 2, 4, 6, and 8 weeks with controls in each group. As a result of chronic exercise there was an increase in the width of the zona fasciculata of the adrenal cortex. Also, there was an increase in the number and size of the mitochondria, and an increase in the quant...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215882
更新日期：1976-05-06 00:00:00
abstract：:Frozen sections of growing terminal follicles of the locust ovary were incubated with an ecdysteroid-specific rabbit antibody and the bound antibody visualised by the use of FITC-labelled goat-anti-rabbit antiserum. A bright fluorescence was seen in the cytoplasm of the follicle cells in terminal follicles with a leng...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220391
更新日期：1978-11-20 00:00:00
abstract：:The glandular lobe of the corpus cardiacum (CC) of Locusta migratoria contains secretory cells and axon terminals. It is generally accepted that the glandular cells produce an adipokinetic hormone which stimulates the release of diglycerides from the fat body. The axon terminals are supposed to be involved in the regu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00223069
更新日期：1977-10-26 00:00:00
abstract：:It is clear that the well-described phenomenon of epithelial-mesenchymal transition (EMT) plays a pivotal role in embryonic development, wound healing, tissue regeneration, organ fibrosis and cancer progression. EMTs have been classified into three subtypes based on the functional consequences and biomarker context in...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-011-1227-1
更新日期：2012-01-01 00:00:00
abstract：:The antral hormone gastrin is the key regulator of gastric acid secretion, mucosal growth and differentiation. Gastrin is synthesized in the endocrine G-cells in the antroduodenal mucosa. We have now examined the way in which the loss of gastrin alone or gastrin plus cholecystokinin (CCK) affects the antral G-cell. Im...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-1110-z
更新日期：2005-07-01 00:00:00
abstract：:Dysfunction of the retinal pigmented epithelium (RPE) is one of the first effects of dry age-related macular degeneration (AMD) with consequent blindness. Hence, patients affected by this retinal disorder could benefit from a cell-based transplantation strategy for RPE. Actually, an effective protocol to approach this...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-015-2185-9
更新日期：2015-10-01 00:00:00
abstract：:Changes in intestinal function, notably impaired transit, following ischemia/reperfusion (I/R) injury are likely to derive, at least in part, from damage to the enteric nervous system. Currently, there is a lack of quantitative data and methods on which to base quantitation of changes that occur in enteric neurons. In...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-010-1126-x
更新日期：2011-04-01 00:00:00
abstract：:Ornithine decarboxylase (ODC) activity was measured in homogenates of neonatal rabbit ovaries from the day of birth to day 22, an interval that corresponds to the progressing stages of the meiotic prophase from oogonia to diplotene and follicle formation. The ODC-activity was high on days 1 and 3 when most germ cells ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218114
更新日期：1983-01-01 00:00:00
abstract：:Whether an axon will regenerate after it is cut depends on the balance between the intrinisic ability of the axon to regrow and the permissiveness of the environment surrounding it. The permissiveness of the environment is determined by the glial cells at the site of injury, and in the CNS both oligodendrocytes and as...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410050943
更新日期：1997-11-01 00:00:00
abstract：:In the central nervous system of the male domestic flow, metenkephalin (ENK) immunoreactive perikarya and fiber tracts as well as extensive but sharply delimited fiber networks were visualized by means of the PAP technique. The most striking results were: (1) The demonstration of an association of ENK-containing struc...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236920
更新日期：1980-01-01 00:00:00
abstract：:The isolated perfused heart model was used to examine the structure of rat cardiac capillaries and their permeability to macromolecules of various sizes. Haemoglobin (diameter 6.4 nm) and catalase (10.4 nm) did not cross the endothelium but remained on the luminal side. Cytochrome C (3 nm) and horseradish peroxidase (...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213826
更新日期：1988-04-01 00:00:00
abstract：:Connective tissue microfibrils were observed in tissues prepared with methods believed to minimize the loss of tissue components. The eyes of C57BL/6J mice were fixed with glutaraldehyde followed by either freeze substitution, or embedding in glycol methacrylate, a water-miscible embedding medium, after limited or no ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318486
更新日期：1995-02-01 00:00:00
abstract：:Germ cells of human fetuses aged 7-13 weeks were studied. Dense fibrous material called nuage was found in the cytoplasm of human fetal germ cells. The nuage increased in frequency in the germ cells during the interval studied. In oogonia the nuage occurred much more frequently than in spermatogonia and the frequency ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209243
更新日期：1978-11-09 00:00:00
abstract：:Modulation of the actin cytoskeleton in chondrocytes has been used to prevent or reverse dedifferentiation and to enhance protein synthesis. We have hypothesized that an actin-modifying agent, staurosporine, could be used with fibrochondrocytes to increase the gene expression and synthesis of critical fibrocartilage p...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0705-6
更新日期：2008-12-01 00:00:00
abstract：:We have tested the suggestion that the reported increase, in hypoxic rats, in the number of lung endocrine cells immunoreactive for the regulatory peptide CGRP is caused by an accumulation of peptide within the cells which renders them more detectable, rather than by a real increase in proliferation. The incorporation...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00338049
更新日期：1992-04-01 00:00:00
abstract：:Membrane-coating granules of the epithelium of the hamster cheek pouch displayed a lamellated internal structure consisting of alternating thick and thin electron dense bands separated by translucent bands of equal width. The repeat distance of the contents was 8.5 nm +/- sd. 0.7 nm and the thickness of the major elec...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00224374
更新日期：1978-02-24 00:00:00
abstract：:Black tiger shrimps (Penaeus monodon) are able to survive and can be reared under various salinities, possibly by the cellular adaptation of their excretory system, particularly the antennal gland, which is known to regulate body fluid in crustaceans. We have investigated the morphological and biochemical alterations ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-010-0971-y
更新日期：2010-06-01 00:00:00
abstract：:Dental caries is a chronic, infectious, and destructive disease that allows bacteria to break into the dental pulp tissue. As caries-related bacteria invade the human dentinal tubules, odontoblasts are the first line of dental pulp that trigger the initial inflammatory and immune responses. DNA methylation is a key ep...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-02993-0
更新日期：2019-06-01 00:00:00
abstract：:Stereological studies showed that treatment of normal adult human adrenocortical cells in primary culture with ACTH or cyclic-AMP for 2 days results in similar increases in the volume of cells, of the mitochondrial and "membrane space" compartments and of the surface area of the smooth endoplasmic reticulum and mitoch...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218169
更新日期：1978-07-05 00:00:00
abstract：:Identified dorsal unpaired median (DUM) neurones of the locust Locusta migratoria were stained intracellularly with large amounts of cobalt to reveal their extensive peripheral branching patterns. Two neurones of the suboesophageal ganglion were studied as well as several neurones of thoracic ganglia. The peripheral b...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050970
更新日期：1997-12-01 00:00:00
abstract：:Immunocytochemistry using antibodies against Met-enkephalin and Leu-enkephalin has demonstrated a group of large enkephalin-immunoreactive neurons in the nucleus of the rostral mesencephalic tegmentum (mRMT) of two teleost fish. Salmo gairdneri and Salmo salar. Injections of cobalt-lysine in the medial optic tectum re...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00305731
更新日期：1991-04-01 00:00:00
abstract：:The passage of cells across the lymphatic endothelium of rat lacteals in both normal and non-pathological experimental conditions (fasting, lymphatic, stasis) was studied by means of serial thin sections and three-dimensional models. Two different pathways of transendothelial migration were observed: (1) macrophages e...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327761
更新日期：1990-10-01 00:00:00
abstract：:In developing bioassays for estrogenic effects, vitellogenin (Vtg) induction and zona radiata protein (Zr-protein) induction in males and juveniles of oviparous vertebrates have been used as sensitive biomarkers for estrogenicity. Nonylphenol (NP) produces similar and parallel expression patterns of Vtg and Zr-protein...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0543-y
更新日期：2008-03-01 00:00:00
abstract：:The immunohistochemical distribution of the basement membrane (BM) proteins, laminin and type IV collagen, and interstitial type III collagen was investigated in 12 fetal spleens at the 15th-38th gestational weeks (g.w.) and in spleens of 8 infants from term to 4 years. The results were compared with the distribution ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318766
更新日期：1991-02-01 00:00:00
abstract：:Drosophila melanogaster photoreceptors are highly polarized cells and their plasma membrane is organized into distinct domains. Zonula adherens junctions separate a smooth peripheral surface, the equivalent of the basolateral surface in other epithelial cells, from the central surface (approximately equal to apical su...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000195
更新日期：2000-05-01 00:00:00
abstract：:Male rats kept on a standard diet were treated either with progesterone or testosterone by a single intramuscular injection of preparations which are slowly absorbed and metabolized. The rats were anaesthetized 24 h after application of the hormones, perfused with glutaraldehyde, and the parathyroid glands prepared fo...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318797
更新日期：1992-05-01 00:00:00
abstract：:Interstitial cells of Cajal (ICC) are important regulatory cells in the smooth muscle coats of the digestive tract. Expression of the Kit receptor tyrosine kinase was used in this study as a marker to study their distribution and development in the striated musculature of the mouse esophagus. Sections and whole-mounts...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100418
更新日期：2001-10-01 00:00:00
abstract：:Peptidyl-glycine alpha-amidating monooxygenase (PAM; EC 1.14.17.3) is an enzyme that catalyzes conversion of glycine-extended peptides to alpha-amidated bioactive peptides. Two peptides that are processed at their carboxyl-termini by this enzyme are neuropeptide Y and anglerfish peptide Y, both of which possess a C-te...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00304521
更新日期：1995-04-01 00:00:00
