Abstract：
:The effect of a physiological dose of vitamin C (100 microg/ml) on goat articular cartilage chondrocytes cultured in an alginate matrix and subjected to static pressurization of 2.4 MPa was investigated. Biochemical analyses of DNA, glycosaminoglycan (GAG), collagen and protease activity were carried out in various matrix fractions, i.e. cellular matrix (CM) and further removed matrix (FRM), and in culture medium. The treatment of chondrocytes with vitamin C after static pressure increased the GAG content in both CM and FRM (P < 0.03) as compared with control or vitamin C/ static load alone. The collagen content of chondrocytes treated with vitamin C alone and vitamin C after static load also increased significantly in FRM (P < 0.003) as compared with control and static load alone. The specific activity of protease in CM and FRM decreased after vitamin C supplementation both with and without static pressure relative to control (P < 0.003). Transmission electron-microscopic images showed a mixed population of spherical and elliptical chondrocytes when vitamin C was added after static load as compared with static load alone where only elliptical cells were seen. Abundant pericellular and collagen fibrils were seen in this group of chondrocytes as compared with all other groups and the control. The results thus show that, in vitro, vitamin C supplementation of chondrocytes after static loading has the potential to reduce the morphological and biochemical degeneration of chondrocytes caused by static loading, thereby improving the cellular health and functioning of articular cartilage.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Sharma G,Saxena RK,Mishra Pdoi
10.1007/s00441-008-0666-9subject
Has Abstractpub_date
2008-10-01 00:00:00pages
111-20issue
1eissn
0302-766Xissn
1432-0878journal_volume
334pub_type
杂志文章abstract：:Fragments of pituitary tissue obtained from a total of 37 patients with either breast cancer, diabetic retinopathy, galactorrhea, or acromegaly were dissociated into single cell suspensions prior to cell culture. Release of human growth hormone (hGH) and human prolactin (hPRL) into the culture medium was measured by r...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219803
更新日期：1978-08-16 00:00:00
abstract：:The wall of the seminiferous tubule in rodents consists of an inner layer of myoid cells covered by an outer layer of endothelial cells. Myoid cells are a type of smooth muscle cell containing α-actin filaments arranged in two independent layers that contract when stimulated by endothelin-1. The irregular surface reli...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-016-2386-x
更新日期：2016-08-01 00:00:00
abstract：:Previous neurohistological studies have been extended to include the structures contained solely or mainly within the junctional esophageal segment which may play an important role in the sphincter mechanism. The main findings were: 1) a progressive cranio-caudal thickening of the muscularis mucosae; 2) a conspicuous ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215998
更新日期：1976-05-26 00:00:00
abstract：:The existence of the surface-connected canalicular system (SCCS) has been demonstrated in semithick sections of the frog thrombocytes by the use of a high voltage electron microscope. The SCCS of the thrombocytes in Rana catesbeiana and Rana nigromaculata consists of numerous canaliculi and vesicles with a diameter of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00237001
更新日期：1979-10-01 00:00:00
abstract：:The ontogeny of gut innervation in the anuran amphibian Xenopus laevis was studied using immunohistochemistry on sections of whole larvae from NF stages 38-52. Immunoreactivity to acetylated tubulin confirmed the presence of nerve fibres as early as stages 38-39. Actin immunoreactivity was found at stage 41, indicatin...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100423
更新日期：2001-10-01 00:00:00
abstract：:Ultrastructural studies of pancreatic islets have suggested that crinophagy provides a possible mechanism for intracellular degradation of insulin in the insulin-producing B-cells. In the present study, a quantitative estimation of crinophagy in mouse pancreatic islets was attempted by morphometric analysis of lysosom...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213820
更新日期：1988-04-01 00:00:00
abstract：:This study aims to illustrate potential transport mechanisms behind the divergent approaches to nitrogen excretion seen in the ureotelic toadfish (Opsanus beta) and the ammoniotelic plainfin midshipman (Porichthys notatus). Specifically, we wish to confirm the expression of a urea transporter (UT), which is found in t...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1591-0
更新日期：2013-06-01 00:00:00
abstract：:The genes encoding transmembrane glycoproteins of the cadherin family, i.e., the Ca(2+)-dependent cell-cell adhesion molecules, are typically expressed in cell-type- or cell-lineage-specific patterns. One of them, vascular endothelial (VE)-cadherin, is widely considered to be specific for vascular endothelia in which ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-008-0718-1
更新日期：2009-01-01 00:00:00
abstract：:The relationship between nitric oxide synthase (NOS)- and galanin-immunoreactive nerve terminals and the origin of NOS-immunoreactive nerve terminals on the motor endplates in the striated muscles of the rat esophagus was investigated. Double immunohistochemical staining revealed a dual innervation of motor endplates ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051230
更新日期：1999-02-01 00:00:00
abstract：:It is possible to determine statistically the repartitions of shapes or sizes in a population of particles from the recurrence of shapes or sizes of their outlines in microscopical sections. This method was applied to a population of 1326 follicular profiles randomly sampled from 30 ovaries of pubescent homozygous "Nu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219416
更新日期：1976-07-30 00:00:00
abstract：:We have previously demonstrated that tooth size is determined by dental mesenchymal factors. Exogenous bone morphogenetic protein (BMP)4, Noggin, fibroblast growth factor (FGF)3 and FGF10 have no effect on tooth size, despite the expressions of Bmp2, Bmp4, Fgf3, Fgf10 and Lef1 in the dental mesenchyme. Among the wingl...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1224-4
更新日期：2011-09-01 00:00:00
abstract：:Type 1 diabetes is an autoimmune disorder characterized by a lack of insulin production by the beta cells of the pancreas. This lack of insulin causes a variety of systemic effects on whole-body metabolism. Poorly managed type 1 diabetes can lead to cardiovascular disease, diabetic neuropathy, and diabetic retinopathy...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-012-1387-7
更新日期：2012-09-01 00:00:00
abstract：:The etiologic basis of Parkinson's disease (PD), the second most common age-related neurodegenerative disorder, is unknown. Recent epidemiological and experimental studies indicate that exposure to environmental agents, including a number of agricultural chemicals, may contribute to the pathogenesis of this disorder. ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-004-0937-z
更新日期：2004-10-01 00:00:00
abstract：:Kruppel-like factor 4 (KLF4) is a zinc-finger-type transcription factor with a restricted expression pattern during skeletal development. We have previously shown that KLF4 represses osteoblast mineralization concomitant with a down-regulation in the expression of a number of osteoblastic genes, both in vivo and in vi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-1931-8
更新日期：2014-10-01 00:00:00
abstract：:Examination of pituitaries from young and adult turtles representing four families, reveals that in addition to the abundant juxtaneural pars tuberalis (juxPT) found in this class of reptiles, there is generally a substantial amount of pars tuberalis (PT) tissue closely associated with the pars distalis (PD). The PT f...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218290
更新日期：1982-01-01 00:00:00
abstract：:Our immunocytochemical investigation of the magnocellular neuroendocrine system in the goldfish hypothalamus reveals enkephalin (ENK)-containing neurons interspersed among the vasotocin (VT)- and isotocin (IT)-containing neurons of the preoptic nucleus. The perikarya of the ENK, VT, and IT neurons do not show distinct...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236894
更新日期：1979-08-03 00:00:00
abstract：:The antigen detected by the rat anti-mouse monoclonal antibody (m Ab), anti-BSP-3, has been initially described as a brain cell-surface protein. Evidence is presented that this m Ab recognizes mouse (Na+ + K+)-ATPase (ATP phosphohydrolase, E.C.3.6.1.3). The antigen, purified from mouse brain by means of affinity chrom...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218655
更新日期：1983-01-01 00:00:00
abstract：:The influence of adrenalectomy and administration of hypertonic saline on the amount of vasopressin, oxytocin, and neurophysin contained in the median eminence and the neural lobe of rats was studied by means of the following methods: (i) morphometric and microphotometric analyses of aldehyde fuchsin-stained histologi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00206271
更新日期：1983-01-01 00:00:00
abstract：:Luteinizing hormones-releasing hormone (LHRH), vasopressin, and corticotropin systems were examined by immunocytochemical methods in male rats 2 to 20 days after deafferentation of the basal hypothalamus. Axonal degeneration of the vasopressin system (whose perikarya lie rostral to the island) and the corticotropin sy...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210157
更新日期：1981-01-01 00:00:00
abstract：:Dental pulp stem cells (DPSCs) are an attractive alternative mesenchymal stem cell (MSC) source because of their isolation simplicity compared with the more invasive methods associated with harvesting other MSC sources. However, the isolation method to be favored for obtaining DPSC cultures remains under discussion. T...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1630-x
更新日期：2013-07-01 00:00:00
abstract：:In the central nervous system, serotonergic and dopaminergic signaling is terminated by the activity of specialized transporter proteins for serotonin (SERT) and dopamine (DAT). These transporter proteins are found both on the cell surface and in intracellular transport vesicles. Trafficking between these subcellular ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1256-9
更新日期：2011-11-01 00:00:00
abstract：:The present review describes several lines of recent evidence providing new insights into the basic principles and mechanisms of axon projection from the olfactory epithelium to the olfactory bulb. Olfactory sensory neurons are classified into approximately 1000 subtypes according to the expression of specific odorant...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410050953
更新日期：1997-11-01 00:00:00
abstract：:An investigation of the stereospecificity of beta-adrenergic insulin release, its relation to alpha-adrenergic blockade and the adrenergic innervation of the pancreatic islets was performed in the mouse. It was observed that in vivo beta-adrenergic stimulation of insulin release by isopropylnoradrenaline was stereospe...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221602
更新日期：1978-10-06 00:00:00
abstract：:Although the pharmacological properties and distribution of inositol 1,4,5-trisphosphate (IP3)-sensitive calcium stores vary considerably among tissues, studies of its localization have been devoted mostly to the central nervous system. In this report we have analyzed the localization of IP3 receptors in diverse non-n...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050641
更新日期：1996-08-01 00:00:00
abstract：:Whether an axon will regenerate after it is cut depends on the balance between the intrinisic ability of the axon to regrow and the permissiveness of the environment surrounding it. The permissiveness of the environment is determined by the glial cells at the site of injury, and in the CNS both oligodendrocytes and as...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410050943
更新日期：1997-11-01 00:00:00
abstract：:Their strategic location between blood and tissue and their constitutive properties allow endothelial cells (EC) to monitor the transport of plasma molecules, by employing bidirectional receptor-mediated and receptor-independent transcytosis and endocytosis, and to regulate vascular tone, cellular cholesterol and lipi...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-008-0678-5
更新日期：2009-01-01 00:00:00
abstract：:Adenohypophysial primordia of rat embryos at 13 to 15 days gestation were cultured in Parker 199 synthetic medium for 2 to 11 days. At the end of the culture period their fine structure and the presence of immunoreactive trophic hormones using the peroxidase-labeled antibody technique were investigated. The degree of ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00224303
更新日期：1976-07-26 00:00:00
abstract：:The protein tyrosine phosphatase interacting protein 51 (PTPIP51) is thought to regulate crucial cellular functions such as mitosis, apoptosis, migration, differentiation and communication between organelles as a scaffold protein. These diverse functions are modulated by the tyrosine/serine phosphorylation status of P...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-016-2508-5
更新日期：2017-06-01 00:00:00
abstract：:Osteoclasts are highly polarized cells from both morphological and functional points of view. Using quick-freeze, rotary-replication methods combined with cell-shearing, we clarified the variability of cytoplasmic surface of the polarized membranes of osteoclasts seeded on apatite. As to the organization of actin fila...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-2085-4
更新日期：2015-05-01 00:00:00
abstract：:From its inception, tissue engineering has had three tenets: cells, biomaterial scaffolds and signaling molecules. Among the triad, cells are the center piece, because cells are the building blocks of tissues. For decades, cell therapies have focused on the procurement, manipulation and delivery of healthy cells for t...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-012-1339-2
更新日期：2012-03-01 00:00:00
