Abstract:
:Although several studies have shown a dysregulation of microRNA (miRNA) expression profiles in cutaneous melanoma, there has been little research on the miRNA machinery itself. In this study, we investigated the mRNA expression profiles of different miRNA machinery components in primary cutaneous malignant melanoma (PCMM), cutaneous malignant melanoma metastases (CMMM) and benign melanocytic nevi (BMN). Patients with PCMM (n = 7), CMMM (n = 6) and BMN (n = 7) were included in the study. Punch biopsies were harvested from the centers of tumors (lesional) and from BMN (control). In contrast to previous reports exploring specific clusters of miRNAs in PCMM, the present study investigates mRNA expression levels of Dicer, Drosha, Exp5, DGCR8 and the RISC components PACT, argonaute-1, argonaute-2, TARBP1, TARBP2, MTDH and SND1, which were detected by TaqMan real-time reverse transcription polymerase chain reaction (RT-PCR). Argonaute-1, TARBP2 and SND1 expression levels were significantly higher in BMN compared to PCMM (p < 0.05). TARBP2 expression levels were significantly higher in CMMM compared to PCMM (p < 0.05). SND1 expression levels were significantly higher in CMMM compared to PCMM and BMN (p < 0.05). Dicer, Drosha, DGCR8, Exp5, argonaute-2, PACT, TARBP1 and MTDH expression levels showed no significant differences within groups (p > 0.05). The results of this study show that the miRNA machinery components argonaute-1, TARBP2 and SND1 are dysregulated in PCMM and CMMM compared to BMN and may play a role in the process of malignant transformation.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Sand M,Skrygan M,Georgas D,Sand D,Gambichler T,Altmeyer P,Bechara FGdoi
10.1007/s00441-012-1446-0subject
Has Abstractpub_date
2012-10-01 00:00:00pages
119-26issue
1eissn
0302-766Xissn
1432-0878journal_volume
350pub_type
杂志文章abstract::Caprine preantral follicles within ovarian fragments were cryopreserved in the absence or presence of 0.5 M sucrose with or without 1 M dimethyl sulfoxide and/or 1 M ethylene glycol (EG). After being thawed, they were washed in minimum essential medium with or without 0.3 M sucrose. Histological analysis of follicle i...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0193-5
更新日期:2006-09-01 00:00:00
abstract::The origin and renewal of the argentaffin cells in the pyloric glands of hamsters were studied by flash, cumulative and pulse labelling autoradiography with 3H-thymidine. The argentaffin cells were identified by the Diazo Method using Fast Red B Salt. By flash labelling autoradiography, it was shown that the argentaff...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222771
更新日期:1977-06-20 00:00:00
abstract::Transmission electron microscopy was used to study the effects of proteolytic enzymes (collagenase, trypsin, clostripain), the calcium chelator ethyleneglycol-bis-(beta-aminoethyl ether) N,N,N',N'-tetraacetic acid (EGTA), and the calcium ionophore A 23187 on substrate adhesion and fine structure of chondrocytes and fi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00228443
更新日期:1984-01-01 00:00:00
abstract::Marked changes in mice pubic symphysis occur by the end of pregnancy. Tissue remodeling involves a dynamic balance between cell proliferation and programmed cell death as well as changes in the extracellular matrix components. Therefore, it is important to consider both of these cellular behaviors when investigating t...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-007-0463-x
更新日期:2007-10-01 00:00:00
abstract::The young of the aquatic Australian frog, Rheobatrachus silus (Leptodactylidae) develop from eggs to juvenile frogs in the mother's stomach. During brooding the stomach expands greatly and becomes very thin walled. Transmission electron microscopy showed that the distension of the stomach was accompanied by a severe d...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222194
更新日期:1983-01-01 00:00:00
abstract::Dysfunction of the retinal pigmented epithelium (RPE) is one of the first effects of dry age-related macular degeneration (AMD) with consequent blindness. Hence, patients affected by this retinal disorder could benefit from a cell-based transplantation strategy for RPE. Actually, an effective protocol to approach this...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-015-2185-9
更新日期:2015-10-01 00:00:00
abstract::The effects of thyroidectomy, adrenalectomy, and castration on the pars distalis of male Japanese quail, and of injection of LH-RH on sexually inactive females, were investigated by light and electron microscopy. Correlation between light and electron microscopy was attained by use of alternate thin and thick sections...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219152
更新日期:1975-06-09 00:00:00
abstract::The antral hormone gastrin is the key regulator of gastric acid secretion, mucosal growth and differentiation. Gastrin is synthesized in the endocrine G-cells in the antroduodenal mucosa. We have now examined the way in which the loss of gastrin alone or gastrin plus cholecystokinin (CCK) affects the antral G-cell. Im...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-1110-z
更新日期:2005-07-01 00:00:00
abstract::Cells binding anti-bovine TASH beta serum were found exclusively in the rostral lobe of the adenohypophysis of the drake using the peroxidase-antiperoxidase complex unlabelled antibody method. The specificity of the binding of the anti-serum to TSH cells was established by relating the morphology and relative abundanc...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00234834
更新日期:1979-04-30 00:00:00
abstract::Gastrin stimulates gastric acid secretion by acting on the cholecystokinin B/gastrin receptor (CCK-BR). The localization of this receptor at the cellular level showed conflicting results in animal studies and has not been described in man by immunohistochemistry. The aim of the present study is to characterize the pre...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004419900114
更新日期:2000-02-01 00:00:00
abstract::In mouse hepatocytes, the gap junctional proteins connexin32 (Cx32) and connexin26 (Cx26) are expressed in the same gap junctional plaque. Expression of the major Cx32 protein is downregulated during liver regeneration and cholestasis. Here we have analyzed the acute-phase response (after experimental inflammation) an...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410000177
更新日期:2000-04-01 00:00:00
abstract::The postnatal maturation of regions of the epididymis and intragonadal segment of the deferens duct was studied in the rat by light- and transmission electron microscopy. Maturation of the genital duct starts in the distal cauda epididymidis and ductus deferens after one week of life, and one week later, in the more c...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215508
更新日期:1987-08-01 00:00:00
abstract::Using anti-carp-gonadotropic-gamma-globulin, the indirect immunofluorescence technique was applied to sections of the pituitary of 12 teleost species. From the investigated species, the Poeciliinae, and especially the black molly, showed a distinct localization of fluorescent cells in the mesoadenohypophysis. Strong f...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224328
更新日期:1976-03-16 00:00:00
abstract::Calbindin is a calcium-binding protein, commonly found in certain subpopulations of the enteric nervous system in mammals. Recently, calbindin-immunoreactive enteric neurons have also been demonstrated in shorthorn sculpin (Myoxocephalus scorpius). In the present study, calbindin immunoreactivity has been investigated...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-011-1135-4
更新日期:2011-04-01 00:00:00
abstract::Captopril [1-(D-3-mercapto-2-methyl-1-oxopropyl)-L-proline (S,S)] is now known to be an inhibitor of angiotensin-converting enzyme. In male mice, the juxtaglomerular cells were examined by electron microscopy 42 h after administration of captopril. Numerous dense granules appeared in the rough endoplasmic reticulum of...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00209980
更新日期:1981-01-01 00:00:00
abstract::In recent years, data have accumulated suggesting that the role of neurotrophins and Trk receptors may not be limited to the nervous system, and the presence of these substances has been detected in a variety of vertebrate and invertebrate non-nervous tissues. This study was designed to map the expression of immunorea...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410000181
更新日期:2000-04-01 00:00:00
abstract::Fluorescent (aminergic) fibers were demonstrated by the Falck-Hillarp technique in all parts of the neurohypophysis (NH) in the roach (Leuciscus rutilus). The fibers are very thin and few in number in the rostral (RNH) and proximal neurohypophysis (PNH) but slightly more numerous in the NH adjacent to the pars interme...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219959
更新日期:1975-01-01 00:00:00
abstract::The presence, location and degree of immunoexpression of various microfilament (MF) and intermediate filament (IF) systems (actin, cytokeratins, desmin, vimentin) were studied in the excurrent ducts of the testis in sexually mature and active galliform (Japanese quail, domestic fowl, turkey) and anseriform (duck) bird...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-008-0631-7
更新日期:2008-08-01 00:00:00
abstract::We compared the bone regeneration potentials of autologous cultured osteoblasts and of bone-marrow-derived autologous MSCs in combination with allogeneic cancellous bone granules in a rabbit radial defect model. Radial shaft defects over 15 mm were made in 26 New Zealand white rabbits. The animals underwent insertion ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-011-1272-9
更新日期:2012-02-01 00:00:00
abstract::Glycoproteins rich in mannosyl or glucosyl residues were analyzed in the subcommissural organ (SCO) and the pineal organ of the sheep (Ovis aries). By use of concanavalin A labelled with fluorescein isothiocyanate, fluorescent material was found both in ependymal and hypendymal cells of the SCO. In the pineal organ, e...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218562
更新日期:1986-01-01 00:00:00
abstract::An attempt has been made to reveal which of the nine different types of synapses in the fundus striati, discriminated in a previous study, degenerate following experimental lesions in the parafasciculo-center median complex of the cat. Two types of synaptic contacts were found to be degenerated two days after the lesi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00226045
更新日期:1976-09-06 00:00:00
abstract::For the study of the hypothalamo-hypophysial system of Xenopus laevis tadpoles, hypothalamic lesions were made by means of the electrocoagulation technique. Lesioning of the ventral region of the preoptic nucleus resulted in a decrease of the number of ACTH cells in the pars distalis of the pituitary gland and in a di...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219304
更新日期:1976-06-11 00:00:00
abstract::In model experiments with the use of horseradish peroxidase (HRP), two pathways of transport of substances to the adenohypophysis were studied, as well as the distribution of the tracer in the latter organ. The first pathway allows the tracer to penetrate from the intercellular milieu of the median eminence below the ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214248
更新日期:1984-01-01 00:00:00
abstract::Interstitial cells of Cajal (ICCs) are believed to be a major element in generating the spontaneous rhythm of the gastrointestinal tract. A prominent problem in the study of these cells has been the difficulty in observing them in intact tissues. We used the lipophilic dye DiI to stain ICCs in the submucosal-circular ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051058
更新日期:1998-05-01 00:00:00
abstract::Chronic tissue injury with fibrosis results in the disruption of tissue architecture, organ dysfunction and eventual organ failure. Therefore, the development of effective anti-fibrotic therapies is urgently required. During fibrogenesis, complex interplay occurs between cellular and extracellular matrix components of...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-016-2407-9
更新日期:2016-09-01 00:00:00
abstract::By using an antiserum raised against a crustacean beta-pigment-dispersing hormone (PDH), the distribution and chemical neuroanatomy of PDH-like immunoreactive neurons was investigated in the central nervous system of the gastropod snails, Helix pomatia and Lymnaea stagnalis. The number of immunoreactive cells in the H...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051240
更新日期:1999-02-01 00:00:00
abstract::We previously demonstrated that GLUT2, a facilitated-diffusion glucose transporter isoform known to play critical roles in the regulation of systemic blood glucose level, is present at the apical ends of Müller cells in the rat retina. As a means of elucidating the ontogeny and possible role(s) of GLUT2 in the develop...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004419900099
更新日期:1999-11-01 00:00:00
abstract::Among heterogeneous S100β-protein-positive (S100β-positive) cells, star-like cells with extended cytoplasmic processes, the so-called folliculo-stellate cells, envelop hormone-producing cells or interconnect homophilically in the anterior pituitary. S100β-positive cells are known, from immunohistochemistry, to emerge ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-015-2256-y
更新日期:2016-02-01 00:00:00
abstract::The ontogeny of protein gene product 9.5 (PGP 9.5), serotonin (5-HT), calcitonin gene-related peptide (CGRP), and calcitonin (CT) immunoreactivity was evaluated in small-granule endocrine cells of hamster laryngotracheal epithelium from fetal day 11 to adulthood. Two centrifugal (proximal-to-distal) patterns of differ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00305382
更新日期:1994-01-01 00:00:00
abstract::Live Merkel cells in the skin and hair follicles are known to incorporate a fluorescence dye, quinacrine, which has been utilized to identify and dissect the cells for experiments. Quinacrine fluorescence of the cells is, however, quickly lost and quinacrine-stained Merkel cells soon become difficult to identify in ti...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-002-0688-7
更新日期:2003-03-01 00:00:00