Abstract：
:Among heterogeneous S100β-protein-positive (S100β-positive) cells, star-like cells with extended cytoplasmic processes, the so-called folliculo-stellate cells, envelop hormone-producing cells or interconnect homophilically in the anterior pituitary. S100β-positive cells are known, from immunohistochemistry, to emerge from postnatal day (P) 10 and to proliferate and migrate in the parenchyma of the anterior pituitary with growth. Recent establishment of S100β-GFP transgenic rats expressing specifically green fluorescent protein (GFP) under the control of the S100β-promoter has allowed us to observe living S100β-positive cells. In the present study, we first confirmed that living S100β-positive cells in tissue cultures of S100β-GFP rat pituitary at P5 were present prior to P10 by means of confocal laser microscopy and that they proliferated and extended their cytoplasmic processes. Second, we examined the expression of the Snail-family zinc-finger transcription factors, Snail and Slug, to investigate the mechanism behind the morphological changes and the proliferation of S100β-positive cells. Interestingly, we detected Slug expression in S100β-positive cells and its increase together with development in the anterior pituitary. To analyze downstream of SLUG in S100β-positive cells, we utilized specific small interfering RNA for Slug mRNAs and observed that the expression of matrix metalloprotease (Mmp) 9, Mmp14 and chemokine Cxcl12 was down-regulated and that morphological changes and proliferation were decreased. Thus, our findings suggest that S100β-positive cells express Slug and that its expression is important for subsequent migration and proliferation.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Horiguchi K,Fujiwara K,Tsukada T,Yako H,Tateno K,Hasegawa R,Takigami S,Ohsako S,Yashiro T,Kato T,Kato Ydoi
10.1007/s00441-015-2256-ysubject
Has Abstractpub_date
2016-02-01 00:00:00pages
513-24issue
2eissn
0302-766Xissn
1432-0878pii
10.1007/s00441-015-2256-yjournal_volume
363pub_type
杂志文章abstract：:The organization of the system of perivascular space around the capillaries in the neurohypophysis was studied in the adult and developing laboratory mouse by the use of histological silver impregnation and electron microscopical techniques. In the median eminence short and long extensions, arising mainly from the sha...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00237730
更新日期：1979-06-08 00:00:00
abstract：:The ultrastructure of the calcium-sensitive (Ca-s) (PAS-positive) cells of the pars intermedia was investigated in eels kept in hypo- and hyperosmotic environments. Although the cells were moderately active in fresh water (FW), they were highly stimulated in deionized water (DW) and displayed an enlarged Golgi apparat...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210015
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219870
更新日期：1984-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217555
更新日期：1985-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
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doi：10.1007/BF00217405
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF01740777
更新日期：1990-03-01 00:00:00
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journal_title：Cell and tissue research
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doi：10.1007/BF00318767
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0046-7
更新日期：2006-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318486
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220328
更新日期：1976-10-13 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215454
更新日期：1988-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218954
更新日期：1987-12-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051370
更新日期：1999-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1998-12-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-1839-3
更新日期：2014-07-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216510
更新日期：1984-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327761
更新日期：1990-10-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2018-11-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0562-7
更新日期：2002-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2005-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0004-4
更新日期：2005-11-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2018-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234755
更新日期：1980-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215192
更新日期：1986-01-01 00:00:00
