Abstract：
:In mouse hepatocytes, the gap junctional proteins connexin32 (Cx32) and connexin26 (Cx26) are expressed in the same gap junctional plaque. Expression of the major Cx32 protein is downregulated during liver regeneration and cholestasis. Here we have analyzed the acute-phase response (after experimental inflammation) and circadian connexin expression in Cx32-deficient and wild-type mouse liver. Acute-phase response was triggered by intraperitoneal injection of lipopolysaccharide (LPS). Injection of recombinant mouse interleukin-1beta (mIL-1beta), mIL-6 or tumor necrosis factor alpha (mTNF-alpha) had no inflammatory effect. Northern blot analysis of positive and negative acute-phase transcripts following stimulation with cytokine or LPS revealed no difference between Cx32-deficient livers and wild-type controls, suggesting that loss of the Cx32 gene had no effect on experimental liver inflammation. Actin, beta-fibrinogen and Cx26 transcripts were increased after endotoxin stimulation. Under conditions of hepatic acute-phase response, Cx32 transcripts were not detected in LPS-treated livers of wild-type mice. Immunoblot analysis of proteins from inflamed wild-type livers indicated a strongly diminished amount of Cx32 protein, whereas the level of Cx26 protein was increased. Although intraperitoneal injection of mIL-1, mIL-6 as well as mTNF-alpha did not induce an acute-phase response, Cx32 protein expression was diminished, suggesting that post-transcriptional downregulation of Cx32 preceded the acute-phase response. Northern blot hybridization of RNA from wild-type and Cx32-deficient mouse liver revealed a similar circadian regulation of Cx26 and GAPDH transcripts with maximal expression around 2 p.m. and a minimum after midnight.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Temme A,Ott T,Haberberger T,Traub O,Willecke Kdoi
10.1007/s004410000177keywords:
subject
Has Abstractpub_date
2000-04-01 00:00:00pages
111-7issue
1eissn
0302-766Xissn
1432-0878journal_volume
300pub_type
杂志文章abstract：:Recently, an antibody against the choline transporter (CHT), an essential molecule involved in ACh uptake, was used to label cholinergic nerves in the central nervous system; however, the enteric nervous system (ENS) was not examined. The present study localised CHT immunoreactivity (CHT-IR) within the rat ileum ENS a...
journal_title：Cell and tissue research
pub_type： 杂志文章
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abstract：:During developmental critical periods, external stimuli are crucial for information processing, acquisition of new functions or functional recovery after CNS damage. These phenomena depend on the capability of neurons to modify their functional properties and/or their connections, generally defined as "plasticity". Al...
journal_title：Cell and tissue research
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更新日期：2012-07-01 00:00:00
abstract：:Early in postnatal life, the first wave of spermatogenesis is accompanied by an initial wave of germ cell apoptosis. This may reflect an adjustment in the number of germ cells that can be adequately maintained by Sertoli cells. Two major pathways (intrinsic and extrinsic) are involved in the process of caspase activat...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0186-4
更新日期：2006-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：2003-12-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2014-10-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2004-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1983-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2001-10-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2006-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234830
更新日期：1979-04-30 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210898
更新日期：1982-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215777
更新日期：1983-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0308-z
更新日期：2007-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF01258491
更新日期：1982-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218373
更新日期：1982-01-01 00:00:00
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2002-11-01 00:00:00
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：1999-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221867
更新日期：1986-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1980-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1998-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2016-11-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2004-12-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209243
更新日期：1978-11-09 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2012-06-01 00:00:00
abstract：:We report here on cholinergic neurons in the rat hippocampal formation that were identified by immunocytochemistry employing a monoclonal antibody against choline acetyltransferase (ChAT), the acetylcholine-synthesizing enzyme. In general, ChAT-immunoreactive cells were rare, but were observed in all layers of the hip...
journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1986-01-01 00:00:00
