Abstract：
:The choroid plexus from the lateral ventricles of 18-day chick embryos was cultivated as an organ in medium 199 until the degeneration of the stroma. Selected plexuses forming an empty epithelial sac were then incubated with enzyme-treated human immunoglobulin (5S-antibody) and with native human immunoglobulin (7S-antibody). Uptake of the 7S-antibody was observed after 30 min, whereas the 5S-antibody was taken up by the choroid plexus within 1 min, as demonstrated by means of the peroxidase-anti-peroxidase (PAP) technique (Sternberger 1974). The antibodies were located in conspicuous, large vacuoles of the choroid epithelium. Further experiments were performed using only 5S-antibody. In addition to the demonstration of the protein structure of this immunoglobulin, it was also shown that its binding capacity for tetanus toxoid as an antigen remains intact in the intracellular location. It was not possible to observe lysosomal degradation. Moreover, 5S-antibody was detectable in cultures first incubated with 5S-antibody for 30 min and subsequently in antibody-free medium for a further period of 7 to 11 days. The biological significance of the uptake of material from the cerebrospinal fluid and the possibility of the existence of a receptor for 5S-antibody are discussed.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Vollerthun R,Möller Wdoi
10.1007/BF00237886subject
Has Abstractpub_date
1980-01-01 00:00:00pages
393-410issue
3eissn
0302-766Xissn
1432-0878journal_volume
213pub_type
杂志文章abstract：:The ultrastructure of the rectal papillae of the parasitoid hymenopteran, Nasonia vitripennis (Walk), is described. These organs in this insect consist of four distinct cell types arranged as a closed, hollow cone. The majority of the cells are present in the raised cone, and are characterised by large numbers of mito...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220008
更新日期：1975-08-25 00:00:00
abstract：:Mast cells (MCs) and airway nerves play an important role in allergic asthma. However, little is known about the MCs and their interaction with airway nerves during allergic airway inflammation. This study aims to investigate the distribution and proliferation of MC populations in different lung compartments, along wi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-017-2597-9
更新日期：2017-08-01 00:00:00
abstract：:A synthetic 17-mer peptide corresponding to an unique sequence in the amino-terminal region of human creatine kinase B was used to raise a new and highly B-subunit-specific monoclonal antibody, CK-BYK/21E10. We show here that the monoclonal antibody is suitable for immunohistochemistry of unfixed frozen sections as we...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00307817
更新日期：1995-05-01 00:00:00
abstract：:A monospecific antibody against beta-1,3-glucan recognition protein (a 62 kDa protein) of the larval silkworm prophenoloxidase activating system was used to study the localization of the protein. Among tissues from 5th instar larvae, only hemocytes and plasma were shown to contain a 62 kDa polypeptide immunoreactive w...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00319149
更新日期：1992-06-01 00:00:00
abstract：:Modulation of the actin cytoskeleton in chondrocytes has been used to prevent or reverse dedifferentiation and to enhance protein synthesis. We have hypothesized that an actin-modifying agent, staurosporine, could be used with fibrochondrocytes to increase the gene expression and synthesis of critical fibrocartilage p...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0705-6
更新日期：2008-12-01 00:00:00
abstract：:In fresh-water turtles, the bridge connecting the proximal and caudal stumps of transected spinal cords consists of regenerating axons running through a glial cellular matrix. To understand the process leading to the generation of the scaffold bridging the lesion, we analyzed the mitotic activity triggered by spinal i...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1173-y
更新日期：2011-06-01 00:00:00
abstract：:The distribution of neuropeptide Y (NPY)-like immunoreactivity in the brain of the bichir, Polypterus senegalus, was examined immunohistochemically. NPY-like immunoreactivity was distributed widely in the brain, with the highest density in the diencephalon. NPY-positive perikarya were found in various areas, including...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050874
更新日期：1997-08-01 00:00:00
abstract：:An indirect immunofluorescence study of the frozen sections of the spleen of an anuran amphibian, Xenopus laevis, showed that lymphocytes bearing a small amount of immunoglobulin (Ig) were localized mostly in the white pulp of non-immunized toads. There were fewer fluorescent cells in the red pulp. In the toads hyperi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218363
更新日期：1982-01-01 00:00:00
abstract：:Drosophila melanogaster photoreceptors are highly polarized cells and their plasma membrane is organized into distinct domains. Zonula adherens junctions separate a smooth peripheral surface, the equivalent of the basolateral surface in other epithelial cells, from the central surface (approximately equal to apical su...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000195
更新日期：2000-05-01 00:00:00
abstract：:The distribution of somatostatin and cocaine and amphetamine-regulated transcript (CART) was investigated in rat intracardiac ganglia. Somatostatin immunoreactivity was only present in nerve terminals, always colocalised with choline acetyltransferase immunoreactivity, surrounding approximately 10% of intracardiac neu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0087-y
更新日期：2006-04-01 00:00:00
abstract：:Regenerated adrenocortical nodules were obtained by implanting fragments of the capsular tissue of excised adrenal glands into the musculus gracilis of rats (Belloni et al. 1990). Five months after the operation, operated rats showed a normal basal blood level of corticosterone, but a very low concentration of circula...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318136
更新日期：1991-07-01 00:00:00
abstract：:(1) The basiconic sensilla on the antennae of Calliphora resemble other insect epidermal sensilla; one or several bipolar sense cells are surrounded by three non-neural cells. (2) The apical cell membrane of the tormogen cell (one of the three accessory cells) forms microvilli coated internally with particles. (3) In ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209140
更新日期：1978-06-08 00:00:00
abstract：:Neurons displaying FMRFamide(Phe - Met - Arg - Phe - NH2)-like immunoreactivity have recently been implicated in neural plasticity in salmon. We now extend these findings by describing the extent of the FMRF-like immunoreactive (FMRF-IR) system in the brain, retina and olfactory system of sockeye salmon parr using the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00313518
更新日期：1990-09-01 00:00:00
abstract：:Neural lobes of rats subjected to dehydration by drinking 2% saline for four days were examined electron microscopically and compared to untreated controls. The ultrastructure of the blood vessels and the tissues surrounding them were examined and it was found that, although few exocytotic figures could be seen in eit...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221710
更新日期：1975-06-24 00:00:00
abstract：:Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuropeptide with highly potent neurotrophic and neuroprotective effects. PACAP and its receptors occur in the retina and PACAP has been applied in animal models of metabolic retinal disorders to reduce structural and functional damage. Furthermore, PACAP...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1349-0
更新日期：2012-04-01 00:00:00
abstract：:A combination of light, transmission and scanning electron microscopy was employed to demonstrate the occurrence, arrangement and structure of taste buds in the oral mucosa of the soft palate of monkeys (Macaca irus). Taste buds are found in aggregates confined to 0.15 to 0.3 mm wide, round islands of keratinizing epi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236359
更新日期：1979-01-30 00:00:00
abstract：:Expression of the non-muscle myosin heavy chain-B (NM-MHC-B, also denoted as the embryonic smooth muscle myosin heavy chain, SMemb) was examined in rat urinary bladder during growth in response to a partial urinary outflow obstruction. Following obstruction, the weight of the urinary bladder increased more than five-f...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000262
更新日期：2001-05-01 00:00:00
abstract：:Regeneration of rat mast cells was studied by TEM from 10 s to 48 h after secretion of histamine induced by compound 48/80. During the first 2 h, small intracellular cavities, formed during compound exocytosis and containing non-membrane-bound remnants of the granules, tended to coalesce, and after 2 h of incubation r...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00238658
更新日期：1981-01-01 00:00:00
abstract：:Agitation of villi evokes reflexes that affect the motility of the guinea-pig small intestine. NK1 receptor endocytosis was used to investigate the possible involvement of tachykinins acting on neuronal NK1 receptors in these reflexes. Segments of guinea-pig ileum were incubated at 37 degrees C in Krebs physiological ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051032
更新日期：1998-04-01 00:00:00
abstract：:It is possible to determine statistically the repartitions of shapes or sizes in a population of particles from the recurrence of shapes or sizes of their outlines in microscopical sections. This method was applied to a population of 1326 follicular profiles randomly sampled from 30 ovaries of pubescent homozygous "Nu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219416
更新日期：1976-07-30 00:00:00
abstract：:While brain-derived neurotrophic factor (BDNF) messenger RNA (mRNA) has been localized in the developing gustatory epithelium, little information is available about neurotrophin-3 (NT-3) mRNA expression pattern in the prenatal developing gustatory and lingual epithelium. In the present study, using in situ hybridizati...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051092
更新日期：1998-06-01 00:00:00
abstract：:Vasodilator-stimulated phosphoprotein (VASP) and mammalian-enabled protein (MENA) share similar cellular localisation and functions (signal transduction pathways, regulation of actin cytoskeleton dynamics). Functional substitution and compensation among Ena/VASP proteins have been proposed as the reason for the absenc...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0964-9
更新日期：2004-12-01 00:00:00
abstract：:The endoneurial collagen sheath around teased nerve fibres following crush injury was studied by scanning electron microscopy and compared with uninjured sciatic nerve fibres and with fibres from the dystonic mutant mouse. Following crush injury the endoneurial collagen became more abundant than seen in untreated nerv...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210469
更新日期：1981-01-01 00:00:00
abstract：:Intra-arterial injections of synthetic double-stranded RNA (polyinosinic:polycytidylic acid, PIPC) at a dose of 500 microg/kg evoked pronounced fever in guinea-pigs. PIPC-induced fever could be antagonized by treatment with the non-selective cyclooxygenase (COX) inhibitor diclofenac and was, in part, attenuated by the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0386-6
更新日期：2007-06-01 00:00:00
abstract：:Angiogenesis, the growth of new blood vessels, is required for a variety of normal proliferative processes. Furthermore, angiogenesis is well established as also playing an important role in neoplastic growth and metastasis. Numerous regulators of angiogenesis have been identified and characterized over the last few d...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-008-0675-8
更新日期：2009-01-01 00:00:00
abstract：:The distribution of P2X(2) purine receptor subunit immunoreactivity has been investigated in the mouse gastrointestinal tract. Immunoreactivity occurred in intraganglionic laminar endings (IGLEs) associated with myenteric ganglia throughout the gastrointestinal tract. In the esophagus, IGLEs supplied every myenteric g...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-003-0715-3
更新日期：2003-05-01 00:00:00
abstract：:Low-temperature-embedded tissue of the subcommissural organ (SCO) of the rabbit was analyzed for the basal route of secretory product by means of indirect immuno-metal cytochemistry (protein A-gold technique) at the electron-microscopic level. By use of (1) an antiserum against bovine Reissner's fibre (see Sterba et a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219221
更新日期：1986-01-01 00:00:00
abstract：:The spermatogonial stem cell line in prepubertal and adult bovine testis was studied by electron microscopy and protein gene product 9.5 immunohistochemistry. Three successive spermatogonia precursor cell configurations were observed. Small basal stem cells were found to possess a spherical shape and nuclei with two t...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00583394
更新日期：1995-08-01 00:00:00
abstract：:Peptidyl-glycine alpha-amidating monooxygenase (PAM; EC 1.14.17.3) is an enzyme that catalyzes conversion of glycine-extended peptides to alpha-amidated bioactive peptides. Two peptides that are processed at their carboxyl-termini by this enzyme are neuropeptide Y and anglerfish peptide Y, both of which possess a C-te...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00304521
更新日期：1995-04-01 00:00:00
abstract：:The distribution of MAP2 and actin in dendritic spines of the visual and cerebellar cortices, dentate fascia, and hippocampus was determined by using immunogold electron microscopy. By this approach, we have confirmed the presence of MAP2 in dendritic spines and identified substructures within the spine compartment sh...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00225592
更新日期：1989-06-01 00:00:00
