Abstract:
:Survival of mesenchymal stem cells (MSCs) against oxidative stress and inflammation is vital for effective stem cell therapy. The reactive oxygen species (ROS) result in apoptosis and release of inflammatory mediators. Adipose-derived stem cells (ASCs) have shown promise for stem cell therapy owing to their anti-inflammatory and anti-oxidant activity. Previously, we showed the benefits of vitamin E against hydrogen peroxide (H2O2)-induced oxidative stress in rat bone marrow-derived MSCs. In this study, we aim to evaluate the effect of vitamin E treatment on porcine adipose-derived mesenchymal stem cells (pASCs) against H2O2-induced oxidative stress. The oxidative stress was induced by treating pASCs with 500 μM H2O2 with or without vitamin E. Viability of pASCs is enhanced after vitamin E treatment. In addition, reduced cellular toxicity, total NO level, PGE2 production and caspase-3 activity were observed after vitamin E treatment. Gene expression analysis of vitamin E-treated pASCs showed down-regulated expression for the genes associated with oxidative stress and apoptosis, viz., NOS2, Casp3, p53, BAX, MDM2, NFκB, HIF1α and VEGF-A genes. On the other hand, expression of anti-apoptotic and survival genes was up-regulated, viz., BCL2, BCL2L1 and MCL1. Furthermore, phosphorylation of Akt was attenuated following vitamin E treatment. The findings of this study may help in developing effective stem cell therapy for the diseases characterized by the oxidative stress and inflammation.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Bhatti FUR,Kim SJ,Yi AK,Hasty KA,Cho Hdoi
10.1007/s00441-018-2857-3subject
Has Abstractpub_date
2018-10-01 00:00:00pages
111-120issue
1eissn
0302-766Xissn
1432-0878pii
10.1007/s00441-018-2857-3journal_volume
374pub_type
杂志文章abstract::The motility patterns of the reticulorumen evoke mainly mixing of the ingesta. So far unknown, intrinsic neural circuits of the enteric nervous system are involved in the control of these motility patterns. The aim of the study was to characterize neurochemically sheep ruminal myenteric neurones, in particular the neu...
journal_title:Cell and tissue research
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abstract::The livers of Xenopus laevis, grouped by chronological age (0.5, 2 and 3 yrs), were studied electron microscopically. Ultrastructurally most of the melanin granules in the mature female liver showed an internal structure similar to the melanin granules of the oocytes. The hepatic melanin granules of immature females a...
journal_title:Cell and tissue research
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abstract::Frozen sections of growing terminal follicles of the locust ovary were incubated with an ecdysteroid-specific rabbit antibody and the bound antibody visualised by the use of FITC-labelled goat-anti-rabbit antiserum. A bright fluorescence was seen in the cytoplasm of the follicle cells in terminal follicles with a leng...
journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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doi:10.1007/s004410100380
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224134
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051058
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00210342
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
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doi:10.1007/s004410051042
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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更新日期:2013-04-01 00:00:00