Abstract：
:Conventional two-dimensional cell monolayers do not provide the geometrical, biochemical and mechanical cues found in real tissues. Cells in real tissues interact through chemical and mechanical stimuli with adjacent cells and via the extracellular matrix. Such a highly interconnected communication network extends along all three dimensions. This architecture is lost in two-dimensional cultures. Therefore, at least in many cases, two-dimensional cell monolayers do not represent a suitable in vitro tool to characterize accurately the biology of real tissues. Many studies performed over the last few years have demonstrated that the differences between three-dimensional and two-dimensional cultured cells are striking at the morphological and molecular levels and that three-dimensional cell cultures can be employed in order to shrink the gap between real tissues and in vitro cell models. End-point and long-term imaging of cellular and sub-cellular processes with fluorescence microscopy provides direct insight into the physiological behavior of three-dimensional cell cultures and their response to chemical or mechanical stimulation. Fluorescence imaging of three-dimensional cell cultures sets new challenges and imposes specific requirements concerning the choice of a suitable microscopy technique. Deep penetration into the specimen, high imaging speed and ultra-low intensity of the excitation light are key requirements. Light-sheet-based fluorescence microscopy (LSFM) offers a favorable combination of these requirements and is therefore currently established as the technique of choice for the study of three-dimensional cell cultures. This review illustrates the benefits of cellular spheroids in the life sciences and suggests that LSFM is essential for investigations of cellular and sub-cellular dynamic processes in three-dimensions over time and space.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Pampaloni F,Ansari N,Stelzer EHdoi
10.1007/s00441-013-1589-7subject
Has Abstractpub_date
2013-04-01 00:00:00pages
161-77issue
1eissn
0302-766Xissn
1432-0878journal_volume
352pub_type
杂志文章,评审abstract：:In mouse hepatocytes, the gap junctional proteins connexin32 (Cx32) and connexin26 (Cx26) are expressed in the same gap junctional plaque. Expression of the major Cx32 protein is downregulated during liver regeneration and cholestasis. Here we have analyzed the acute-phase response (after experimental inflammation) an...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000177
更新日期：2000-04-01 00:00:00
abstract：:The skeletal body muscle of vertebrates is derived from segmentally arranged mesodermal structures, the somites. Only the dorsal epithelial half of the somite, the dermomyotome, gives rise to muscle cells during normal development. Head muscle takes its origin from the somites, the unsegmented paraxial head mesoderm a...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410051281
更新日期：1999-04-01 00:00:00
abstract：:Previous neurohistological studies have been extended to include the structures contained solely or mainly within the junctional esophageal segment which may play an important role in the sphincter mechanism. The main findings were: 1) a progressive cranio-caudal thickening of the muscularis mucosae; 2) a conspicuous ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215998
更新日期：1976-05-26 00:00:00
abstract：:The young of the aquatic Australian frog, Rheobatrachus silus (Leptodactylidae) develop from eggs to juvenile frogs in the mother's stomach. During brooding the stomach expands greatly and becomes very thin walled. Transmission electron microscopy showed that the distension of the stomach was accompanied by a severe d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222194
更新日期：1983-01-01 00:00:00
abstract：:Post-translational glycosylation of proteins with O-linked β-N-acetylglucosamine (O-GlcNAcylation) and changes of galectin expression profiles are essential in many cellular stress responses. We examine this regulation in the liver tissue of hibernating thirteen-lined ground squirrels (Ictidomys tridecemlineatus) repr...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03185-x
更新日期：2020-07-01 00:00:00
abstract：:Perinatal changes in the appearance of intramembranous particles (IMPs) of microvilli of enterocytes were analyzed quantitatively. In both the jejunum and the ileum, the IMP density on the P-face showed no significant changes from day 17 of gestation to day 5 of postnatal life. It increased between day 5 and day 12, r...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00212224
更新日期：1983-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219726
更新日期：1976-04-28 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00261829
更新日期：1989-08-01 00:00:00
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journal_title：Cell and tissue research
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doi：10.1007/BF00318179
更新日期：1991-11-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
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更新日期：2012-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1788-2
更新日期：2014-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1135-4
更新日期：2011-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-017-2666-0
更新日期：2017-11-01 00:00:00
abstract：:The hydrozoan medusa Aglantha digitale (Müller 1776) has eight syncytical giant motor axons, up to 40 micrometers in diameter, running from the margin, up the inside of the bell towards the apex. Giant motor axons injected with Lucifer Yellow CH are connected with lateral neurons running circumferentially across the s...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF01258491
更新日期：1982-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00235169
更新日期：1979-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0136-6
更新日期：2006-07-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218939
更新日期：1987-12-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051184
更新日期：1998-11-01 00:00:00
abstract：:The number, size and structure of tendon organs were examined in leg muscles of the rat 3-19 weeks after de-efferentation performed in newborn animals by removal of the lumbosacral spinal cord. After this operation, tendon organs differentiated and grew in disused muscles and were innervated by primary sensory neurons...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214645
更新日期：1985-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00237001
更新日期：1979-10-01 00:00:00
abstract：:The development of the heterophil granulocytes in the bone marrow of the guinea pig is described. During the maturation of these cells, three types of granule are formed, not only the azurophil and specific granules already described in other mammals but also a third type of granule referred to here as the nucleated g...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220388
更新日期：1978-11-20 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1999-12-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210157
更新日期：1981-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1985-01-01 00:00:00
abstract：:Wnts are secreted glycoproteins that play multiple roles in early development, including the differentiation of precursor cells. During this period, gradients of Wnts and other morphogens are formed and regulate the differentiation and migration of neural progenitor cells. Afterwards, Wnt signalling cascades participa...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-014-1996-4
更新日期：2015-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0856-z
更新日期：2004-04-01 00:00:00
abstract：:In this immunocytochemical study we investigated the distribution of nervous structures in the lower lip of adult rats. The region is characterized by a rich cutaneous and mucosal sensory innervation originating from terminal branches of the trigeminal system. Lower lip innervation was investigated by detection of the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051348
更新日期：1999-08-01 00:00:00
abstract：:Human endothelial progenitor cells (EPCs) were isolated from cord blood samples and enriched by magnetic activated cell sorting method based on the CD133 marker. Cells were incubated with different doses of bacterial lipopolysaccharide, ranging from 2, 5, 10, 50, 100, 200, 250, 500, to 1000 µg/ml, for 48 h. The cell s...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03405-4
更新日期：2021-01-18 00:00:00
abstract：:An improved technique for fluorescent labelling of heavy meromyosin has made it possible to detect on a light microscopic level the cellular sites of actin localization. Rabbit heavy meromyosin (HMM) was labelled with fluorescein isothiocyanate so that the actin binding site was protected during the reaction. The spec...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00224134
更新日期：1975-08-27 00:00:00
abstract：:Guanosine triphosphatase (GTPase) activity was studied histo- and cytochemically in the rod outer segments of the rat retina by means of a newly developed method. Differences in the distribution pattern of the enzyme activity exist within the outer segment: the activity is more intense at the tip of the rod outer segm...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217416
更新日期：1983-01-01 00:00:00
