Abstract:
:The present electron microscopic autoradiographic study includes a quantitative analysis of osteoclasts in vitro using tritiated leucin as a protein tracer. A significant increase in the grain density over the ruffled border and the underlying resportion zone was demonstrated two hours post pulse whereas the grain density of the remaining cytoplasm was relatively constant. This indicates a transport of newly synthesized protein from the osteoclast to the extracellular resorption zone. Earlier histochemical and biochemical experiments suggest that the exported protein may represent lysosomal enzymes to be used in the extracellular bone degradation.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Lucht U,Norgaard JOdoi
10.1007/BF00219726keywords:
subject
Has Abstractpub_date
1976-04-28 00:00:00pages
89-99issue
1eissn
0302-766Xissn
1432-0878journal_volume
168pub_type
杂志文章abstract::Olfactory marker protein (OMP) was first described as a protein expressed in olfactory receptor neurons (ORNs) in the nasal cavity. In particular, OMP, a small cytoplasmic protein, marks mature ORNs and is also expressed in the neurons of other nasal chemosensory systems: the vomeronasal organ, the septal organ of Mas...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-020-03349-9
更新日期:2021-01-15 00:00:00
abstract::Considerable evidence indicates that the state of ocular connective tissues and their response in glaucomatous disease affect the degree of glaucoma damage. Both experimental and clinical data suggest that improved diagnostic and prognostic information can be derived from the assessment of the mechanical responsivenes...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-013-1603-0
更新日期:2013-08-01 00:00:00
abstract::The thyroid cells of the cream hamster, characterized by abundance of microtubules and stratification of the organelles, undergo a particular evolution when the animals grow older. These changes are characterized by an increase of the number of lysosomes which in extreme cases become so prominent that they occupy the ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215124
更新日期:1976-02-06 00:00:00
abstract::The effect of hypothyroidism, induced by 4-propyl-2-thiouracil, on muscle satellite cells in vivo and in vitro, and on postnatal muscle fiber development in the soleus muscle of rats during the first 40 days of postnatal life was analyzed. The proliferative activity of satellite cells was determined by means of bromod...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050682
更新日期:1996-10-01 00:00:00
abstract::Maturing erythroblasts and erythrocytes were studied in chickens and adult hens to provide more information on the presence and frequency of various nucleolar types in these cells. Nucleoli were present at all stages of erythroblastic and erythrocytic development except in the case of a few reticulocytes and the matur...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00209211
更新日期:1975-09-17 00:00:00
abstract::Carotid bodies of ducks show no detectable changes in ultrastructure up to periods of four weeks following the removal of 2-3 cm of the cervical vagus (decentralization). This indicates that the majority, and possibily all the nerves terminating on the glomus cells are afferent (sensory) in nature. These nerve endings...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218494
更新日期:1975-11-19 00:00:00
abstract::An immunohistochemical staining technique with the D2-40 antibody was undertaken to examine the functional and morphological features of lymphatic networks in tissue sections and whole-mount preparations of normal nasal mucosa and ethmoid sinus mucosa. In normal nasal mucosa, most lymphatic vessels were found in the s...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0337-7
更新日期:2007-03-01 00:00:00
abstract::We present an experimental method allowing the production of three-dimensional organ-like structures, namely microtissues (MTs), in vitro without the need for exogenous extracellular matrix (ECM) or growth factors. Submandibular salivary glands (embryonic day ED14), kidneys (ED13) and lungs (ED13) were harvested from ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-016-2487-6
更新日期:2016-12-01 00:00:00
abstract::We used a combined biochemical and histocytochemical approach to study ecto-ATPase in the rat cardiac muscle. The reaction medium employed for histocytochemical detection was optimized in biochemical assays to achieve the highest enzyme activity and lowest inhibition by the capture agent used for visualization of the ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004419900105
更新日期:1999-12-01 00:00:00
abstract::Calcitonin gene-related peptide (CGRP) was found extensively in the small intestine of both non-mammalian and mammalian vertebrates using radioimmunoassay and immunocytochemistry. By radioimmunoassay, the levels of CGRP in rats, mice, chickens, bullfrogs and rainbow trout were found to range from 91.5 to 419.1 ng/g ti...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00223142
更新日期:1989-10-01 00:00:00
abstract::The fine structural organization of a cortical filament layer in normal locomoting Amoeba proteus was demonstrated using improved fixation and embedding techniques. Best results were obtained after application of PIPES-buffered glutaraldehyde in connection with substances known to prevent the depolymerization of F-act...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215699
更新日期:1982-01-01 00:00:00
abstract::In humans, pathogenesis in cyclosporine A (CsA)-induced gingival overgrowth (GO) includes the accumulation of extracellular matrix (ECM) constituents, viz., collagen type-1 and type-3 and proteoglycans, in subgingival connective tissue. However, whether this increase is associated with alterations of molecules pivotal...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0200-x
更新日期:2006-09-01 00:00:00
abstract::The two calcium antagonistic agents lanthanum and tetracaine cause severe disturbances in the secretory process of the exocrine pancreas, including inhibition of the rate of protein synthesis and exocytosis. The former effect resulted mainly from the inhibition of amino acid transport. Lanthanum in a concentration up ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00227029
更新日期:1977-05-10 00:00:00
abstract::The bursa of Fabricius of the chicken is known to be both a primary lymphoid organ and a secondary lymphoid tissue. Bursal follicles are equipped with antigen-trapping follicle-associated epithelium. However, bioactive antigens such as protein and bacteria have not been detected in the bursal parenchyma. By immunopero...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-013-1701-z
更新日期:2013-12-01 00:00:00
abstract::We have examined radioautographically the protein synthetic and secretory activity of differentiating odontoblasts and ameloblasts, exposed for 9 h in vitro to various concentrations of colchicine in the presence of 3H-proline. Colchicine impairs the cytodifferentiation of the dental epithelium into ameloblasts and of...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216651
更新日期:1988-06-01 00:00:00
abstract::The presence of 28 kDa calbindin in human odontoblasts was studied by use of specific antibodies raised against chick duodenal 28 kDa calbindin, in immunofluorescence, immuno-peroxidase, and electron-microscopic labelling experiments. The calbindin-like protein was detected mainly in the cytoplasm of odontoblast cell ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00225806
更新日期:1988-11-01 00:00:00
abstract::Fragments of pituitary tissue obtained from a total of 37 patients with either breast cancer, diabetic retinopathy, galactorrhea, or acromegaly were dissociated into single cell suspensions prior to cell culture. Release of human growth hormone (hGH) and human prolactin (hPRL) into the culture medium was measured by r...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219803
更新日期:1978-08-16 00:00:00
abstract::A quantitative evaluation of putative amino acid neurotransmitters in sea pansy polyps by high-performance liquid chromatography indicates that the taurine content exceeds that of other amino acids by a 100-fold. The cellular source of this taurine was investigated by immunohistochemistry with two polyclonal antisera ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050800
更新日期:1997-04-01 00:00:00
abstract::We compare the ultrastructure of the gerbil sublingual gland as seen after cryofixation followed by substitution with osmium tetroxide, with the more familiar appearance of material processed by glutaraldehyde-osmium chemical fixation. After primary cryofixation of fresh salivary glands, the nuclei of the mucous cells...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219075
更新日期:1987-11-01 00:00:00
abstract::Blue-green fluorescent subependymal cells with intraventricular processes were shown by the fluorescent histochemical method to be distributed from the preoptic recess to the infundibular recess of the frog hypothalamus. Electron microscopy revealed at least two types of CSF-contacting subependymal cells, type 1 conta...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221791
更新日期:1977-01-20 00:00:00
abstract::Thyroid hormone (TH) plays an important role in regulating ovarian development. However, the mechanism involved remains unclear. Evidence suggests that glucose is essential for ovarian development, and its uptake is mediated by several glucose transporter proteins (Glut). We have investigated the effects of TH on Glut...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-016-2453-3
更新日期:2016-11-01 00:00:00
abstract::Caprine preantral follicles within ovarian fragments were cryopreserved in the absence or presence of 0.5 M sucrose with or without 1 M dimethyl sulfoxide and/or 1 M ethylene glycol (EG). After being thawed, they were washed in minimum essential medium with or without 0.3 M sucrose. Histological analysis of follicle i...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0193-5
更新日期:2006-09-01 00:00:00
abstract::The cerebral ganglia of the freshwater snail Lymnaea stagnalis contain two clusters of neurosecretory Caudo-Dorsal Cells (CDC). These cells produce a neurohormone which stimulates ovulation. Ganglion transplantation and quantitative electron microscopy show that neuronal isolation of the cerebral ganglia complex (CCC)...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219720
更新日期:1976-04-28 00:00:00
abstract::To investigate the control of the timing in the epithelio-mesenchymal transformation of the neural crest into a migrating population, neural anlagen (neural tube plus crest) were isolated from 2-day quail embryos by proteases in the presence of CA++ and explanted onto substrates favourable for neural crest cell migrat...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218011
更新日期:1985-01-01 00:00:00
abstract::Our immunocytochemical investigation of the magnocellular neuroendocrine system in the goldfish hypothalamus reveals enkephalin (ENK)-containing neurons interspersed among the vasotocin (VT)- and isotocin (IT)-containing neurons of the preoptic nucleus. The perikarya of the ENK, VT, and IT neurons do not show distinct...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00236894
更新日期:1979-08-03 00:00:00
abstract::Caveolae or membrane vesicles are commonly observed in smooth and skeletal muscle as well as in working heart muscle. Using sections of fixed tissue and replicas of freeze-cleaved material, we show in this study that caveolae are also very numerous in sinus node cells of the rabbit, and to a lesser degree, in the atri...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00234869
更新日期:1980-01-01 00:00:00
abstract::Transcriptome profiling of the pineal gland has revealed night/day differences in the expression of a major fraction of the genes active in this tissue, with two-thirds of these being nocturnal increases. A set of over 600 transcripts exhibit two-fold to >100-fold daily differences in abundance. These changes appear t...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-010-1094-1
更新日期:2011-04-01 00:00:00
abstract::The cardiovascular system is the first functional organ in the vertebrate embryo, and many organs start to develop adjacent to cells of the cardiovascular system. Endothelial cells (EC) form the inner cell lining of blood vessels and represent the major cell type that interacts with developing organs. On the one hand,...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-003-0739-8
更新日期:2003-10-01 00:00:00
abstract::Whether environmental exposure to bisphenol A (BPA) may induce reproductive disorders is still controversial but certain studies have reported that BPA may cause meiotic abnormalities in C. elegans and female mice. However, little is known about the effect of BPA on meiosis in adult males. To determine whether BPA exp...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-013-1723-6
更新日期:2014-01-01 00:00:00
abstract::The effect of pilocarpine and food uptake on the rate of incorporation of [3H]-leucine in vivo was measured by means of quantitative radioautography in three exocrine cells of the rat: the acinar and the granular duct cells of the submandibular and the acinar cells of the parotid gland. The three cell types react diff...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219936
更新日期:1975-12-18 00:00:00