Export of protein from the osteoclast as studied by electron microscopic autoradiography.

Abstract:

:The present electron microscopic autoradiographic study includes a quantitative analysis of osteoclasts in vitro using tritiated leucin as a protein tracer. A significant increase in the grain density over the ruffled border and the underlying resportion zone was demonstrated two hours post pulse whereas the grain density of the remaining cytoplasm was relatively constant. This indicates a transport of newly synthesized protein from the osteoclast to the extracellular resorption zone. Earlier histochemical and biochemical experiments suggest that the exported protein may represent lysosomal enzymes to be used in the extracellular bone degradation.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Lucht U,Norgaard JO

doi

10.1007/BF00219726

keywords:

subject

Has Abstract

pub_date

1976-04-28 00:00:00

pages

89-99

issue

1

eissn

0302-766X

issn

1432-0878

journal_volume

168

pub_type

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