Abstract：
:Extracellular matrix (ECM) plays a dynamic role during tissue development and re-growth. Body part regeneration efficiency relies also on effective ECM remodelling and deposition. Among invertebrates, echinoderms are well known for their striking regenerative abilities since they can rapidly regenerate functioning complex structures. To gather insights on the involvement of ECM during arm regeneration, the brittle star Amphiura filiformis was chosen as experimental model. Eight ECM genes were identified and cloned, and their spatio-temporal and quantitative expression patterns were analysed by means of whole mount in situ hybridisation and quantitative PCR on early and advanced regenerative stages. Our results show that almost none of the selected ECM genes are expressed at early stages of regeneration, suggesting a delay in their activation that may be responsible for the high regeneration efficiency of these animals, as described for other echinoderms and in contrast to most vertebrates. Moreover, at advanced stages, these genes are spatially and temporally differentially expressed, suggesting that the molecular regulation of ECM deposition/remodelling varies throughout the regenerative process. Phylogenetic analyses of the identified collagen-like genes reveal complex evolutionary dynamics with many rounds of duplications and losses and pinpointed their homologues in selected vertebrates. The study of other ECM genes will allow a better understanding of ECM contribution to brittle star arm regeneration.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Ferrario C,Czarkwiani A,Dylus DV,Piovani L,Candia Carnevali MD,Sugni M,Oliveri Pdoi
10.1007/s00441-020-03201-0subject
Has Abstractpub_date
2020-09-01 00:00:00pages
411-426issue
3eissn
0302-766Xissn
1432-0878pii
10.1007/s00441-020-03201-0journal_volume
381pub_type
杂志文章abstract：:Previously, we showed that synchronous autophagy occurred in the corpora allata during the declining phase of juvenile hormone synthesis in mated adult females of the cockroach Diploptera punctata. At the onset of this process, conspicuous whorl-like autophagosomes were often observed. Each consisted of a ribosome-con...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050764
更新日期：1997-02-01 00:00:00
abstract：:Light microscopy of native preparations, histology, and electron microscopy have revealed that Phlebotomus duboscqi belongs to a class of sand fly species with prompt development of the peritrophic matrix (PM). Secretion of electron-lucent fibrils, presumably chitin, starts immediately after the ingestion of a blood m...
journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000262
更新日期：2001-05-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-014-2029-z
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abstract：:Brain injuries trigger physiological reactions which are mediated by a number of cytokines including interleukin-6 (IL-6), ciliary neurotrophic factor (CNTF), and leukemia inhibitory factor (LIF). Astrocytes and microglia, the protagonists in these traumatic responses, are known to secrete a variety of paracrine signa...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0624-x
更新日期：2002-11-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214011
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abstract：:The termination of intrafusal muscle fibres was studied in muscle spindles of the tenuissimus muscle of the Chinese hamster. Most nuclear chain fibres terminated within the spindle capsule. The nuclear bag- and nuclear chain fibres that extended beyond the limit of the spindle capsule terminated in the endomysial conn...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218663
更新日期：1983-01-01 00:00:00
abstract：:With the use of immunocytochemistry, it was shown that both the supraoptic and paraventricular hypothalamic nuclei in humans contain at least two different neurophysins. These two human neurophysins are immunologically related to bovine neurophysin I and neurophysin II, respectively. One human neurophysin is associate...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00240096
更新日期：1979-02-15 00:00:00
abstract：:By a regular immunoperoxidase method, alpha-1-proteinase inhibitor (Api) was demonstrated in pancreatic islets in individuals with and without genetic deficiency of Api. Subsequently a double immunoperoxidase method, with two different chromogens (diaminobenzidine-brown and 4-chloro-1-naphthol-blue), was applied on th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218078
更新日期：1986-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00227032
更新日期：1977-05-10 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03185-x
更新日期：2020-07-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-009-0900-0
更新日期：2010-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0186-4
更新日期：2006-09-01 00:00:00
abstract：:Large granular lymphocytes (LGL) comprise a natural defense system in the liver and exert an inhibitory effect on tumor cell metastasis. In order to demonstrate the maturation of LGL in the liver from the morphological aspect, we evaluated electron-microscopically the frequency of 0.2 micron vesicles (rod-cored and "e...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00354799
更新日期：1994-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00309876
更新日期：1990-11-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-008-0678-5
更新日期：2009-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004419900126
更新日期：2000-03-01 00:00:00
abstract：:The distribution and interconnections of brainstem catecholamine cell groups thought to be important in cardiovascular control were studied using histochemical and ultrastructural techniques in the rabbit. Lesions and microinjections of horseradish peroxidase (HRP) were made in the nucleus tractus solitarii in the dor...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209963
更新日期：1981-01-01 00:00:00
abstract：:It is known that human and animal fibroblasts are able to induce the retraction of a fibrin clot. In the present study the correlation between (i) fibrin-fibroblasts during growth, (ii) the number of actin stress-lines in mouse fibroblasts during growth in culture, and (iii) the sensitivity of actin stress-lines to a ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218485
更新日期：1982-01-01 00:00:00
abstract：:The ultrastructure of the calcium-sensitive (Ca-s) (PAS-positive) cells of the pars intermedia was investigated in eels kept in hypo- and hyperosmotic environments. Although the cells were moderately active in fresh water (FW), they were highly stimulated in deionized water (DW) and displayed an enlarged Golgi apparat...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210015
更新日期：1981-01-01 00:00:00
abstract：:The alimentary system of Habrotrocha rosa is composed of the alimentary channel, five digestive glands and another gland which is in close relation to the intestinal syncytium. After the present investigation, the alimentary channel can be divided into sections which clearly differ from each other, these are: oral cav...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209138
更新日期：1978-06-08 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221087
更新日期：1977-02-09 00:00:00
abstract：:The postnatal development of brush cells in the distal segment of the common bile duct of the rat was examined with respect to cell number and immunoreactivity for liver fatty acid-binding protein (L-FABP). The brush cells, distinguishable from the principal cells by scanning electron microscopy, first appeared in the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318592
更新日期：1991-12-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050562
更新日期：1996-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210458
更新日期：1981-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218202
更新日期：1987-05-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215508
更新日期：1987-08-01 00:00:00
abstract：:Caprine preantral follicles within ovarian fragments were cryopreserved in the absence or presence of 0.5 M sucrose with or without 1 M dimethyl sulfoxide and/or 1 M ethylene glycol (EG). After being thawed, they were washed in minimum essential medium with or without 0.3 M sucrose. Histological analysis of follicle i...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0193-5
更新日期：2006-09-01 00:00:00
abstract：:The olfactory sensilla on the maxillary palp tip of Locusta migratoria (L.) resemble the surrounding contact chemoreceptors in general morphology. The perforated peg has a thicker wall than is commonly found in olfactory sensilla. The form and position of the sensilla are considered in relation of the olfactory functi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219899
更新日期：1977-11-07 00:00:00
abstract：:Prolactin cells were identified by means of immunocytochemistry with protein-A gold as a marker on ultrathin sections of the pituitary gland of young (3-4 months), middle-aged (16-19 months), and aged (26-30 months) male Wistar rats. Point-counting volumetry revealed that the prolactin (PRL) cell-volume density in mid...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215844
更新日期：1988-02-01 00:00:00
