Bone morphogenetic protein-2 does not alter the differentiation pathway of committed progenitors of osteoblasts and chondroblasts.

Abstract:

:Bone morphogenetic proteins (BMPs) induce cartilage and bone formation at both bony and non-bony sites. We examined the possibility whether BMP-2 induces differentiation of osteoblast progenitors into chondroblast lineage cells using organ culture and cell culture prepared from the calvaria of newborn mouse. BMP-2 stimulated alkaline phosphatase activity (a marker of osteoblasts) and induced positive alcian blue staining (a marker of chondroblasts) in a dose- and time-dependent manner in cell cultures isolated from the whole calvaria. BMP-2 also increased the number of round-shaped cells in the cell cultures, which expressed type II collagen. Histologically, the calvaria consisted of not only bone, but also cartilaginous tissues stained with alcian blue, which were located along the endocranial surface of the parietal and occipital bones. When the calvariae were organ-cultured in the presence of BMP-2, the territory of the cartilaginous tissue was markedly increased, and covered most of the occipital bone. A histological examination of the cultured calvariae showed that the bony region of the occipital bone remained unchanged, while the cartilaginous region expanded independent of the bony region. BMP-2 increased the number of proliferating chondroblasts only in the cartilaginous tissue, but never induced new cartilage formation at the bony site. We obtained cells from the anterior portion that contained no cartilage and the posterior portion which contained cartilage, and we subsequently cultured them separately. BMP-2 stimulated ALP activity in all the cultures. However, the treatment with BMP-2 increased the intensity of alcian blue staining only in tissue culture of the posterior portion, but never induced alcian blue staining in tissue culture of the anterior portion. These results indicate that the chondrocytes induced by BMP-2 were derived from the cartilaginous tissue, which had already formed at the surface of the calvarial bone. BMP-2 did not induce differentiation of committed osteoblast progenitors into chondroblast lineage cells.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Komaki M,Katagiri T,Suda T

doi

10.1007/s004410050562

subject

Has Abstract

pub_date

1996-04-01 00:00:00

pages

9-17

issue

1

eissn

0302-766X

issn

1432-0878

journal_volume

284

pub_type

杂志文章
  • Effect of neonatal sympathectomy on the postnatal differentiation of the submandibular gland of the rat.

    abstract::Right superior cervical sympathectomy was performed in one-day old rats. This operation had a small but definite effect on the postnatal development of the submandibular gland. The gland on the sympathectomized side weighed less and contained smaller amounts of DNA, RNA and protein than the contralateral intact gland....

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00227032

    authors: Srinivasan R,Chang WW

    更新日期:1977-05-10 00:00:00

  • Annexins in rat enterocyte and hepatocyte: an immunogold electron-microscope study.

    abstract::In the present study, immunogold labeling of ultrathin sections of rat small intestine and liver has been used to obtain insights into the ultrastructural localization and possible functions of annexins. In enterocytes, annexins II, IV, and VI are found at the periphery of the core of each microvillus and of the rootl...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00414181

    authors: Weinman JS,Feinberg JM,Rainteau DP,Gaspera BD,Weinman SJ

    更新日期:1994-11-01 00:00:00

  • The ultrastructure of the protonephridial flame cell of the freshwater planarian Bdellocephala brunnea.

    abstract::The protonephridial flame cell of the freshwater planarian Bdellocephala brunnea was studied by electron microscopy. This basketlike cell with luminal cilia and fenestrae is known as a cyrtocyte. Careful examination revealed the actual continuity of the fenestrae over the surface of the cell and the microvillous natur...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00237973

    authors: Ishii S

    更新日期:1980-01-01 00:00:00

  • Immunohistochemistry of vacuoles occurring in rat hepatocytes after retinol administration.

    abstract::The vacuoles occurring in rat hepatocytes after intraportal injection of retinol (33 or 67 micrograms) were examined immunohistochemically using respective antibodies against rat albumin, human retinol-binding protein, human ceruloplasmin, human alpha 1-antitrypsin, human transferrin, and human prealbumin as represent...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00261829

    authors: Kudo S

    更新日期:1989-08-01 00:00:00

  • The pigmentary system of planaria. II. Physiology and functional morphology.

    abstract::The pigmentary system of the planaria, Dugesia gonocephala s.l. (Platyhelminthes, Turbellaria, Tricladida), consists of granules contained in chromatophore cells distributed in the parenchyma tissue. The administration of MSH release-inhibiting Factor (M.I.F.) leads to an easily observable general decolouration of the...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00236132

    authors: Palladini G,Medolago-Albani L,Margotta V,Conforti A,Carolei A

    更新日期:1979-06-27 00:00:00

  • A new type of gap junction in the phylum Brachiopoda.

    abstract::Gap junctions in the phylum Brachiopoda are shown to be of the P type as has been previously reported in invertebrates only in molluscs and possibly bryozoans. However, the mollusc gap junctions appear identical to vertebrae gap junctions in the fracture face on which particles remain, in particle spacing and in parti...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00206345

    authors: Flower NE,Green CR

    更新日期:1982-01-01 00:00:00

  • Role of notochord cells and sclerotome-derived cells in vertebral column development in fugu, Takifugu rubripes: histological and gene expression analyses.

    abstract::Despite the common structure of vertebrates, the development of the vertebral column differs widely between teleosts and tetrapods in several respects, including the ossification of the centrum and the function of the notochord. In contrast to tetrapods, vertebral development in teleosts is not fully understood, parti...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-016-2404-z

    authors: Kaneko T,Freeha K,Wu X,Mogi M,Uji S,Yokoi H,Suzuki T

    更新日期:2016-10-01 00:00:00

  • Appearance of retrogradely labeled neurons in the rat superior cervical ganglion after injection of wheat-germ agglutinin-horseradish peroxidase conjugate into the contralateral ganglion.

    abstract::Injection of wheat-germ agglutinin-horseradish peroxidase conjugate (WGA-HRP) into the superior cervical ganglion (SCG) of the rat results in accumulation of WGA-HRP in sympathetic postganglionic neurons in the contralateral SCG. The sympathetic pathways involved and the mechanism underlying the labeling were investig...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00327745

    authors: Caner H,Tamamaki N,Handa Y,Hayashi M,Nojyo Y

    更新日期:1990-10-01 00:00:00

  • Developmental immunohistology of melanotrophs in Xenopus laevis tadpoles.

    abstract::The adenohypophysial primordium of Xenopus laevis tadpoles at stages 33/34 to 46 (Nieuwkoop and Faber, 1956) were examined immunohistologically for alpha-MSH, beta-MSH and ACTH. beta-MSH was demonstrated from stage 37/38 onwards, and alpha-MSH from stage 39. No signs of ACTH production were detected. alpha-MSH and bet...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00231955

    authors: Erik N,Nyholm I,Doerr-Schott J

    更新日期:1977-05-16 00:00:00

  • Apoptosis in cardiac development.

    abstract::Cell degeneration, as a phenomenon accompanying developmental processes, was originally described over a century ago. Apoptosis, a term introduced approximately three decades ago, has occupied investigators particularly with respect to cell and tissue kinetics, emphasizing its role in the disposal of supernumerary, ma...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s004410000227

    authors: Poelmann RE,Molin D,Wisse LJ,Gittenberger-de Groot AC

    更新日期:2000-07-01 00:00:00

  • Uptake of ferritin by the cephalopod optic gland.

    abstract::Horse spleen ferritin, injected into the blood of the octopus, leaves the capillaries via pericyte junctions and windows. One hour after the administration, ferritin has entered the optic gland main cells; three hours later, it is accumulated in dense-bodies. The evidence for resorption supports earlier papers reporti...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00361712

    authors: Froesch D,Mangold K

    更新日期:1976-08-10 00:00:00

  • Ultrastructure of the nongranulated cells and morphology of the extracellular spaces in the pars distalis of adult and pouch-young tammar wallabies (Macropus eugenii).

    abstract::Differentiated nongranulated (folliculo-stellate) cells were observed in the centre and periphery of the cords of cells in the pars distalis of the adult tammar wallaby (Macropus eugenii). The nongranulated cells formed follicles containing a small lumen: the cell apices were joined by junctional complexes including z...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00210898

    authors: Leatherland JF,Renfree MB

    更新日期:1982-01-01 00:00:00

  • Electron-cytochemical localization of alkaline phosphatase to G cells of Necturus maculosus antrum.

    abstract::Electron-cytochemical localization of alkaline phosphatase activity was performed on G cells of Necturus maculosus antral mucosa. Alkaline phosphatase activity was localized to the nuclear membrane, the Golgi/endoplasmic reticulum, and the limiting membranes of G cell peptide-secretion vesicles. There was no specific ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00218867

    authors: Rutten MJ,Garrison RG,Moore CD,Fiskin AM,Cheung LY

    更新日期:1989-12-01 00:00:00

  • ChAT and NOS in human myenteric neurons: co-existence and co-absence.

    abstract::Most myenteric neurons contain one of the two generating enzymes for major excitatory and inhibitory neurotransmitters: choline acetyltransferase (ChAT) or neuronal nitric oxide synthase (NOS). Two minor groups of myenteric neurons contain either both enzymes or neither. Our study had two aims: (1) to compare the prop...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-009-0852-4

    authors: Beck M,Schlabrakowski A,Schrödl F,Neuhuber W,Brehmer A

    更新日期:2009-10-01 00:00:00

  • Lysosomes and pancreatic islet function. A quantitative estimation of crinophagy in the mouse pancreatic B-cell.

    abstract::Ultrastructural studies of pancreatic islets have suggested that crinophagy provides a possible mechanism for intracellular degradation of insulin in the insulin-producing B-cells. In the present study, a quantitative estimation of crinophagy in mouse pancreatic islets was attempted by morphometric analysis of lysosom...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00213820

    authors: Schnell AH,Swenne I,Borg LA

    更新日期:1988-04-01 00:00:00

  • Regulation of glucose transport by thyroid hormone in rat ovary.

    abstract::Thyroid hormone (TH) plays an important role in regulating ovarian development. However, the mechanism involved remains unclear. Evidence suggests that glucose is essential for ovarian development, and its uptake is mediated by several glucose transporter proteins (Glut). We have investigated the effects of TH on Glut...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-016-2453-3

    authors: Ding Y,Tian Y,Guo M,Liu J,Heng D,Zhu B,Yang Y,Zhang C

    更新日期:2016-11-01 00:00:00

  • Molecular interactions between neurotrophin receptors.

    abstract::Neurotrophins signal via a dual-receptor system comprising receptor tyrosine kinases, the Trks, and a tumor necrosis factor (TNF) receptor like molecule, p75. Interest in these receptors was spurred on by the finding that they are employed by their neurotrophin ligands to activate opposing cellular mechanisms. Signall...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s004410100378

    authors: Dechant G

    更新日期:2001-08-01 00:00:00

  • Control of the pars intermedia of the lizard, Anolis carolinensis. III. Changes in the ultrastructure of the disconnected neuro-intermediate lobe.

    abstract::Morphological changes in the disconnected neuro-intermediate lobe were studied in the lizard, Anolis carolinensis from the 2nd to the 14th post-operative day using a threefold aldehyde fixative (Rodríguez, 1969). Two phases of colour change capacity were exhibited: Phase I started immediately after the transection, la...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00237724

    authors: Larsson L,Rodriguez EM,Meurling P

    更新日期:1979-06-08 00:00:00

  • Immunolocalization of inhibin alpha-subunit in the human testis. A light- and electron-microscopy study.

    abstract::The localization of inhibin alpha-subunit in the human testis was studied at the light- and electron-microscope level with immunostaining techniques. Antibodies against specific fragments of porcine and human inhibin alpha-subunits were utilized. At light microscopy, inhibin alpha-subunit immunoreactivity was detected...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00319612

    authors: Vannelli GB,Barni T,Forti G,Negro-Vilar A,Vale W,Serio M,Balboni GC

    更新日期:1992-08-01 00:00:00

  • Calretinin-immunoreactive nerves in the uterus, pelvic autonomic ganglia, lumbosacral dorsal root ganglia and lumbosacral spinal cord.

    abstract::Nerves containing the calcium-binding protein calretinin have been reported in several organs but not in female reproductive organs and associated ganglia. This study was undertaken to determine if nerves associated with the uterus contain calretinin and the source(s) of calretinin-synthesizing nerves in the rat (are ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004419900071

    authors: Papka RE,Collins J,Copelin T,Wilson K

    更新日期:1999-10-01 00:00:00

  • The hypothalamo-choroidal tract. I. Immunohistochemical demonstration of neurophysin pathways to telencephalic choroid plexuses and cerebrospinal fluid.

    abstract::Neurosecretory pathways were examined in normal male rats by the use of the immunoperoxidase technique for the localization of neurophysin in Bouin-fixed, deparaffinized sections. Using this technique two projections of extrahypothalamic neuorosecretory fibers can be traced to the sites of origin of the choid plexuses...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00232829

    authors: Brownfield MS,Kozlowski GP

    更新日期:1977-03-01 00:00:00

  • Characterization of the structure, vascularity, and stem/progenitor cell populations in porcine Achilles tendon (PAT).

    abstract::This study aimed to characterize porcine Achilles tendon (PAT) in terms of its structural components, vascularity, and resident tendon cells. We found that PAT is composed of a paratenon sheath, a core of fascicles, and an endotenon/interfascicular matrix (IFM) that encases the fascicle bundles. We analyzed each of th...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-020-03379-3

    authors: Zhang J,Li F,Williamson KM,Tan S,Scott D,Onishi K,Hogan MV,Wang JH

    更新日期:2021-01-26 00:00:00

  • Gastric pentadecapeptide body protection compound BPC 157 and its role in accelerating musculoskeletal soft tissue healing.

    abstract::There is a current need for a therapy that can alleviate the social and economic burden that presents itself with debilitating and recurring musculoskeletal soft tissue injuries and disorders. Currently, several therapies are emerging and undergoing trials in animal models; these focus on the manipulation and administ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s00441-019-03016-8

    authors: Gwyer D,Wragg NM,Wilson SL

    更新日期:2019-08-01 00:00:00

  • Morphological evidence of sperm maturation in the ampulla ductus deferentis of the bull.

    abstract::The present lectin histochemical comparison of cauda epididymal and ampullary bovine sperm was conducted to investigate whether ampullary secretions are involved in altering the plasma-membrane glycoconjugates of sperm. A marked redistribution of glycoconjugates between sperm from these two regions was indeed revealed...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00318822

    authors: Bergerson W,Amselgruber W,Sinowatz F,Bergerson M

    更新日期:1994-03-01 00:00:00

  • Distribution of the Aplysia cardioexcitatory peptide, NdWFamide, in the central and peripheral nervous systems of Aplysia.

    abstract::NdWFamide is an Aplysia cardioexcitatory tri-peptide containing D-tryptophan. To investigate the roles of this peptide, we examined the immunohistochemical distribution of NdWFamide-positive neurons in Aplysia tissues. All the ganglia of the central nervous system (CNS) contained NdWFamide-positive neurons. In particu...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-003-0707-3

    authors: Morishita F,Nakanishi Y,Sasaki K,Kanemaru K,Furukawa Y,Matsushima O

    更新日期:2003-04-01 00:00:00

  • Dose-dependent mechanism of Notch action in promoting osteogenic differentiation of mesenchymal stem cells.

    abstract::Osteogenic differentiation is a tightly regulated process realized by progenitor cell osteoblasts. Notch signaling pathway plays a critical role in skeletal development and bone remodeling. Controversial data exist regarding the role of Notch activation in promoting or preventing osteogenic differentiation. This study...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-019-03130-7

    authors: Semenova D,Bogdanova M,Kostina A,Golovkin A,Kostareva A,Malashicheva A

    更新日期:2020-01-01 00:00:00

  • The fine structure of the centrohelidian heliozoan Heterophrys marina.

    abstract::The fine structure of Heterophrys marina (Centrohelidia, Heliozoa) is described with special reference to centroplast structure, morphogenesis and "behavior" of kinetocysts (= axopodial granules which perform saltatory movement), and formation of organic spicules in a new type of organelle located in the plasma membra...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00222116

    authors: Bardele CF

    更新日期:1975-08-01 00:00:00

  • Morphological and functional changes in the tight junctions of the bile canaliculi induced by bile duct ligation.

    abstract::Thin sections after bile duct ligation showed that the depth of tight junctions appeared to increase and that the distance between individual punctate contacts appeared to become irregular and wider than in controls. The freeze fracture replicas clearly demonstrated these changes in the tight junction morphology. Chan...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00236724

    authors: Koga A,Todo S

    更新日期:1978-12-28 00:00:00

  • Correction to: Immunolocalization and changes of 17beta-estradiol during ovarian development of Chinese mitten crab Eriocheir sinensis.

    abstract::The published online version contains mistake in Title and in Acknowledgement section. Please find below for the needed corrections. ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,已发布勘误

    doi:10.1007/s00441-018-2865-3

    authors: Pan J,Liu M,Chen T,Cheng Y,Wu X

    更新日期:2018-10-01 00:00:00

  • Nitric oxide: a co-modulator of efferent peptidergic neurosecretory cells including a unique octopaminergic neurone innervating locust heart.

    abstract::Our findings suggest that nitric oxide (NO) acts as peripheral neuromodulator in locusts, in which it is commonly co-localized with RF-like peptide in neurosecretory cells. We also present the first evidence for NO as a cardio-regulator in insects. Putative NO-producing neurones were detected in locust pre-genital fre...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-006-0188-2

    authors: Bullerjahn A,Mentel T,Pflüger HJ,Stevenson PA

    更新日期:2006-08-01 00:00:00